##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660689_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26255465 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12339522457515 34.0 31.0 34.0 30.0 34.0 2 32.26496757913067 34.0 31.0 34.0 30.0 34.0 3 32.24992846251247 34.0 31.0 34.0 30.0 34.0 4 35.656405247440865 37.0 35.0 37.0 35.0 37.0 5 35.67628457542077 37.0 35.0 37.0 35.0 37.0 6 35.6969195556049 37.0 35.0 37.0 35.0 37.0 7 35.59506700795434 37.0 35.0 37.0 33.0 37.0 8 35.67258496469211 37.0 35.0 37.0 35.0 37.0 9 37.35674344369829 39.0 38.0 39.0 34.0 39.0 10-11 37.214910476733124 39.0 37.0 39.0 34.0 39.0 12-13 37.325219644748245 39.0 37.0 39.0 34.0 39.0 14-15 38.73826294830429 40.0 38.5 41.0 35.0 41.0 16-17 38.67279236913153 40.0 38.0 41.0 34.0 41.0 18-19 38.5198202736078 40.0 38.0 41.0 34.0 41.0 20-21 38.40997045757902 40.0 38.0 41.0 34.0 41.0 22-23 38.20497903960185 40.0 38.0 41.0 33.5 41.0 24-25 38.23250412818817 40.0 38.0 41.0 34.0 41.0 26-27 38.2619392572175 40.0 38.0 41.0 34.0 41.0 28-29 38.18909383246498 40.0 38.0 41.0 33.5 41.0 30-31 38.05072481100601 40.0 38.0 41.0 33.0 41.0 32-33 37.92538587680698 40.0 38.0 41.0 33.0 41.0 34-35 37.82536344338217 40.0 38.0 41.0 33.0 41.0 36-37 37.612561461013925 40.0 37.5 41.0 32.0 41.0 38-39 37.17344425246325 39.5 35.0 41.0 32.0 41.0 40-41 37.20395525274452 40.0 35.0 41.0 32.0 41.0 42-43 37.03793827304144 40.0 35.0 41.0 31.5 41.0 44-45 36.906892050854935 39.0 35.0 41.0 31.0 41.0 46-47 36.93261349589505 40.0 35.0 41.0 31.0 41.0 48-49 36.91105122305014 40.0 35.0 41.0 31.0 41.0 50-51 35.5438038899711 38.5 34.0 40.0 28.5 40.5 52-53 35.57949444049077 38.5 34.5 39.5 28.0 40.5 54-55 35.831105371776886 39.0 35.0 41.0 26.0 41.0 56-57 35.34623616454708 39.0 35.0 41.0 22.5 41.0 58-59 35.1195526340897 39.0 35.0 41.0 22.0 41.0 60-61 34.948822654635904 39.0 35.0 41.0 22.0 41.0 62-63 34.72902490967118 38.0 34.5 41.0 22.0 41.0 64-65 34.42509346149458 37.5 34.0 40.0 20.5 41.0 66-67 34.05766102409537 37.0 34.0 40.0 20.0 41.0 68-69 33.776660782812264 36.5 34.0 40.0 20.0 41.0 70-71 33.397178016081604 36.0 33.0 39.0 19.0 41.0 72-73 33.08758014759975 35.0 33.0 39.0 19.0 41.0 74-75 32.70579443174973 35.0 33.0 38.5 18.0 40.0 76-77 32.339885372435795 35.0 32.5 37.0 17.5 39.5 78-79 31.97138085347184 35.0 32.0 37.0 16.0 39.0 80-81 31.593340148422435 35.0 32.0 36.0 15.0 39.0 82-83 31.297342039076437 35.0 32.0 36.0 12.5 37.5 84-85 31.008354355940753 35.0 31.0 35.5 11.0 37.0 86-87 30.749110080510857 35.0 31.0 35.0 10.0 37.0 88-89 30.5673281353044 35.0 31.0 35.0 10.0 36.0 90-91 30.37114913028583 35.0 31.0 35.0 10.0 36.0 92-93 30.20898117782336 35.0 30.5 35.0 10.0 36.0 94-95 30.056584752926675 35.0 30.0 35.0 10.0 35.5 96-97 29.912986115462054 34.0 30.0 35.0 10.0 35.0 98-99 29.781318651183668 34.0 30.0 35.0 10.0 35.0 100 29.66406765220117 34.0 29.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35079156267268985 1101 2 0.33213908496843914 1101 3 0.33046630832170365 1101 4 0.28947915864497986 1101 5 0.289652378358916 1101 6 0.2299352263953054 1101 7 0.25600201761130137 1101 8 0.24159621338665005 1101 9 0.33948784327607484 1101 10-11 0.3544128023474258 1101 12-13 0.3309469641877385 1101 14-15 0.4063874663067182 1101 16-17 0.38996914392953386 1101 18-19 0.442287112586051 1101 20-21 0.3781566458986134 1101 22-23 0.3883164528538643 1101 24-25 0.36913142294858403 1101 26-27 0.36479453632001935 1101 28-29 0.36401596717973916 1101 30-31 0.3310622880539782 1101 32-33 0.33979466039020423 1101 34-35 0.33666516189962437 1101 36-37 0.3273674160442539 1101 38-39 0.31479485234570603 1101 40-41 0.27586094444347253 1101 42-43 0.28491718710216674 1101 44-45 0.27582341619177697 1101 46-47 0.29716656093460614 1101 48-49 0.28637691510572694 1101 50-51 0.23335326186995786 1101 52-53 0.28707908585026587 1101 54-55 0.33930292393954886 1101 56-57 0.35894004719346384 1101 58-59 0.32149845470622296 1101 60-61 0.3000772746187934 1101 62-63 0.2858819316811818 1101 64-65 0.24693294615527606 1101 66-67 0.2741249307452449 1101 68-69 0.22875738979137594 1101 70-71 0.2473968315173991 1101 72-73 0.20786624613276672 1101 74-75 0.19047049533578075 1101 76-77 0.16655913936155287 1101 78-79 0.16470628617129535 1101 80-81 0.19923906768046606 1101 82-83 0.12081476099399069 1101 84-85 0.1625825929494411 1101 86-87 0.17101840033169324 1101 88-89 0.1543055541481202 1101 90-91 0.13825355167376685 1101 92-93 0.049724247620570594 1101 94-95 0.08066831544739017 1101 96-97 0.07269161549774594 1101 98-99 0.06159159570229278 1101 100 -0.014158310995064483 1102 1 0.2798344055912878 1102 2 0.2892245993932363 1102 3 0.2892905746584944 1102 4 0.2512321984755985 1102 5 0.2470520392538731 1102 6 0.1618554823694467 1102 7 0.21989716307408713 1102 8 0.19391517943113 1102 9 0.28242090307953305 1102 10-11 0.2897314076650588 1102 12-13 0.27824834191536496 1102 14-15 0.3301188562151012 1102 16-17 0.3570687812690281 1102 18-19 0.3718812995136531 1102 20-21 0.3035471776998264 1102 22-23 0.31262184827377837 1102 24-25 0.31328218500674865 1102 26-27 0.32349520017650946 1102 28-29 0.3075833140702713 1102 30-31 0.3280197144061461 1102 32-33 0.2792499411913383 1102 34-35 0.2896129918537085 1102 36-37 0.25798254447376934 1102 38-39 0.26314203656568935 1102 40-41 0.2349722351792778 1102 42-43 0.2089272667010249 1102 44-45 0.2327570393914371 1102 46-47 0.2402464763027794 1102 48-49 0.2553835609634021 1102 50-51 0.21423238824970525 1102 52-53 0.28886933321313535 1102 54-55 0.3840823590345437 1102 56-57 0.3856820146209614 1102 58-59 0.37225352230434083 1102 60-61 0.34183356963387723 1102 62-63 0.2811024468273473 1102 64-65 0.2618113098808479 1102 66-67 0.27683572898892095 1102 68-69 0.2683264684791311 1102 70-71 0.2226027144319076 1102 72-73 0.20844970974002308 1102 74-75 0.2267558112050807 1102 76-77 0.21041142547954905 1102 78-79 0.19776251210672058 1102 80-81 0.2313207098720298 1102 82-83 0.2540621161112675 1102 84-85 0.2473400938784387 1102 86-87 0.2511213102671199 1102 88-89 0.19905737968375803 1102 90-91 0.23594198339165828 1102 92-93 0.18733470876378178 1102 94-95 0.1756284075911978 1102 96-97 0.15219088517342172 1102 98-99 0.1207068747718445 1102 100 0.1333088421989892 1103 1 0.21757648464758717 1103 2 0.18131768098379553 1103 3 0.2366588826552487 1103 4 0.19122105138993817 1103 5 0.18300344568566373 1103 6 0.12703848668068218 1103 7 0.14554321827582584 1103 8 0.17234794702499556 1103 9 0.20038171403514582 1103 10-11 0.215270897718689 1103 12-13 0.2124772728116966 1103 14-15 0.25977096523531173 1103 16-17 0.2626494939815771 1103 18-19 0.28913589550939633 1103 20-21 0.2563900822252734 1103 22-23 0.277563448184587 1103 24-25 0.23807256853309866 1103 26-27 0.2448219754580805 1103 28-29 0.2536868055936452 1103 30-31 0.1991696635696556 1103 32-33 0.17409791580039524 1103 34-35 0.23704061634921914 1103 36-37 0.20787822317931415 1103 38-39 0.1994045200698764 1103 40-41 0.18640738907618726 1103 42-43 0.14836268796389618 1103 44-45 0.18371029015676044 1103 46-47 0.2829840992921291 1103 48-49 0.2397193501892403 1103 50-51 0.1801685068682204 1103 52-53 0.2467228403583377 1103 54-55 0.33472116065761526 1103 56-57 0.2902197649961735 1103 58-59 0.2854793385692389 1103 60-61 0.2638806664481663 1103 62-63 0.21787946174648454 1103 64-65 0.2251633625076579 1103 66-67 0.24980214396661182 1103 68-69 0.22356495528925535 1103 70-71 0.18922901689025906 1103 72-73 0.15663186316724165 1103 74-75 0.15819756623110948 1103 76-77 0.14932905936905172 1103 78-79 0.16316410298445305 1103 80-81 0.1505023175174145 1103 82-83 0.12771181887661243 1103 84-85 0.12487941487380994 1103 86-87 0.12187083460207404 1103 88-89 0.12279153417270194 1103 90-91 0.1340801083269163 1103 92-93 0.13660659808836684 1103 94-95 0.12310854832523432 1103 96-97 0.09101039521680221 1103 98-99 0.057132238442076044 1103 100 0.04379010420188578 1104 1 0.19597660058147426 1104 2 0.1978900578864895 1104 3 0.1723859807520114 1104 4 0.1990709004473601 1104 5 0.1651343288264968 1104 6 0.10378188894288343 1104 7 0.14114459511420563 1104 8 0.15847910119614284 1104 9 0.2257064136043354 1104 10-11 0.19538971834037966 1104 12-13 0.21120380693960072 1104 14-15 0.2285506918769471 1104 16-17 0.23105449924290866 1104 18-19 0.26151227908315633 1104 20-21 0.2493630693854385 1104 22-23 0.24252056279129874 1104 24-25 0.2195892191370632 1104 26-27 0.23845770907418284 1104 28-29 0.2154955578001463 1104 30-31 0.21409388226150128 1104 32-33 0.20156486392330208 1104 34-35 0.20931707791225307 1104 36-37 0.21721934160992618 1104 38-39 0.20851798083646855 1104 40-41 0.20154840752035597 1104 42-43 0.20338605579805602 1104 44-45 0.18990147068276286 1104 46-47 0.18513480256879689 1104 48-49 0.21334106562783717 1104 50-51 0.20954625868042598 1104 52-53 0.24384119832989626 1104 54-55 0.28983146620436884 1104 56-57 0.37133292402099727 1104 58-59 0.29072537890284167 1104 60-61 0.2679725899061651 1104 62-63 0.246507888779405 1104 64-65 0.2627971785098424 1104 66-67 0.27382847180690106 1104 68-69 0.30406134154862485 1104 70-71 0.27796729869053394 1104 72-73 0.19639451869226576 1104 74-75 0.20746710938765034 1104 76-77 0.20860741627478774 1104 78-79 0.19818243374225375 1104 80-81 0.3024447075289203 1104 82-83 0.26990512012592305 1104 84-85 0.22474655520099063 1104 86-87 0.23993387463547933 1104 88-89 0.24011335020581015 1104 90-91 0.21560723839480644 1104 92-93 0.1885134348826476 1104 94-95 0.19664684824743972 1104 96-97 0.2105444263570071 1104 98-99 0.16247116002419304 1104 100 0.21585590648657416 1105 1 0.14057280794215643 1105 2 0.12218914036809991 1105 3 0.1393043688786264 1105 4 0.13337027089809794 1105 5 0.09504402637174536 1105 6 0.05779090328311298 1105 7 0.060586438521930575 1105 8 0.07336724393073268 1105 9 0.1090404537904135 1105 10-11 0.10576237367012453 1105 12-13 0.10605032416507498 1105 14-15 0.10960891410683615 1105 16-17 0.11246814732799493 1105 18-19 0.14206851036622936 1105 20-21 0.123070132202443 1105 22-23 0.1117307978667057 1105 24-25 0.1105154478980026 1105 26-27 0.10518008893977537 1105 28-29 0.09477259804911853 1105 30-31 0.08920828887332988 1105 32-33 0.07068794684887081 1105 34-35 0.11579675263116229 1105 36-37 0.08456314186435065 1105 38-39 0.11533967819260482 1105 40-41 0.0751432169097015 1105 42-43 0.07748593554845229 1105 44-45 0.030273454095798513 1105 46-47 0.07435523168710034 1105 48-49 0.07105291232161193 1105 50-51 0.00810768449297683 1105 52-53 0.07791005639224835 1105 54-55 0.1739065081660769 1105 56-57 0.15632784272583677 1105 58-59 0.12359020879357985 1105 60-61 0.09854688261211209 1105 62-63 0.1414367271548187 1105 64-65 0.09012513382742782 1105 66-67 0.11281581872851376 1105 68-69 0.09216012239721749 1105 70-71 0.09621587043533708 1105 72-73 0.03396590823824397 1105 74-75 0.03960737286324445 1105 76-77 0.0188741758841644 1105 78-79 0.05624581312826393 1105 80-81 0.08400518880374719 1105 82-83 0.06670960168253615 1105 84-85 0.07592009134720001 1105 86-87 0.0858335028203534 1105 88-89 0.05743984504704969 1105 90-91 0.07356648158101464 1105 92-93 0.05573053091659119 1105 94-95 0.040546038976536636 1105 96-97 0.09312331026697862 1105 98-99 0.0528086095118212 1105 100 0.024564288344670615 1106 1 0.10938513879006706 1106 2 0.10728986922012496 1106 3 0.11586819861236108 1106 4 0.10205688029930826 1106 5 0.08107302712402742 1106 6 0.045327407205810744 1106 7 0.0844525185053584 1106 8 0.07539291051077868 1106 9 0.1150476463518828 1106 10-11 0.07868784526232275 1106 12-13 0.08875312306236793 1106 14-15 0.09876083006891889 1106 16-17 0.11452944932644726 1106 18-19 0.1329374244544752 1106 20-21 0.10538044019030224 1106 22-23 0.1388967457001158 1106 24-25 0.08122640627113498 1106 26-27 0.1383466081367004 1106 28-29 0.08744519529970773 1106 30-31 0.09738801197998725 1106 32-33 0.1215755146051336 1106 34-35 0.0702511803277659 1106 36-37 0.06298667007928316 1106 38-39 0.04995352557826749 1106 40-41 0.07027913110920991 1106 42-43 0.042758796758029405 1106 44-45 0.04916836044777284 1106 46-47 0.048936429864347986 1106 48-49 0.07197054862167107 1106 50-51 -0.01980419690688251 1106 52-53 0.018214127506901434 1106 54-55 0.0925268751935917 1106 56-57 0.11788681029610615 1106 58-59 0.10000109572298044 1106 60-61 0.0683012756411685 1106 62-63 0.050646678505081866 1106 64-65 0.011478354322619566 1106 66-67 0.005678416018646715 1106 68-69 0.028606405065083607 1106 70-71 0.05782646708674832 1106 72-73 0.0014426012780504038 1106 74-75 -0.0337954265495668 1106 76-77 -0.039253827748240155 1106 78-79 0.025882030365799835 1106 80-81 0.05357608378279366 1106 82-83 0.04729683902073489 1106 84-85 0.06769878898053605 1106 86-87 0.04002185656814916 1106 88-89 0.016979755393325746 1106 90-91 0.07518460304586583 1106 92-93 0.053274389366290364 1106 94-95 0.047645333680399915 1106 96-97 0.002715866913135301 1106 98-99 -0.041880624479521344 1106 100 -0.016348385096353724 1107 1 0.03271582730576483 1107 2 0.052396929301004036 1107 3 0.04479346416843555 1107 4 0.06248325412377653 1107 5 0.059032057148399986 1107 6 0.051155909023130164 1107 7 0.0015531514348694486 1107 8 0.011270981479157172 1107 9 0.04056514913975917 1107 10-11 0.03655992314018164 1107 12-13 0.04865623185703072 1107 14-15 0.04030638908467665 1107 16-17 0.027291113526665356 1107 18-19 0.08112732079049323 1107 20-21 0.05432072043838332 1107 22-23 0.022919187835036325 1107 24-25 0.006863424218799707 1107 26-27 0.02568935706867137 1107 28-29 0.07940948994009034 1107 30-31 0.049712250067294406 1107 32-33 0.016844073362868528 1107 34-35 0.030839142931291974 1107 36-37 0.00961386435129441 1107 38-39 0.03286062498857234 1107 40-41 0.007529425079816576 1107 42-43 -0.008629268904485343 1107 44-45 0.019911260044096935 1107 46-47 0.0378255286178657 1107 48-49 -0.027546167801972388 1107 50-51 -0.07031639955379632 1107 52-53 0.05078305238841807 1107 54-55 0.13822367778920608 1107 56-57 0.17093949147279375 1107 58-59 0.17442365141210558 1107 60-61 0.17552491334043907 1107 62-63 0.15026842195232604 1107 64-65 0.1486492611431771 1107 66-67 0.21239110165976882 1107 68-69 0.19730665392439306 1107 70-71 0.18229347382233385 1107 72-73 0.18553560951932013 1107 74-75 0.20865992550635326 1107 76-77 0.177505360428313 1107 78-79 0.18173014268600696 1107 80-81 0.18578148625294943 1107 82-83 0.14553069663601903 1107 84-85 0.17983399457646954 1107 86-87 0.20128301278258576 1107 88-89 0.1239786497681834 1107 90-91 0.1639895403538354 1107 92-93 0.18438094566739593 1107 94-95 0.16130633981222076 1107 96-97 0.15265683202025926 1107 98-99 0.14320076836066065 1107 100 0.1312935125925634 1108 1 0.018869457981217863 1108 2 0.012587032198986492 1108 3 0.033629727866497205 1108 4 0.05835100247765013 1108 5 0.06781891399830187 1108 6 0.06612080835898837 1108 7 0.016708120786972813 1108 8 0.029510914823461576 1108 9 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5508.0 8 15499.0 9 23340.0 10 26888.0 11 27803.0 12 30031.0 13 35058.0 14 43677.0 15 57302.0 16 73506.0 17 91880.0 18 100330.0 19 111327.0 20 128127.0 21 158621.0 22 213596.0 23 318021.0 24 468127.0 25 589423.0 26 601617.0 27 514794.0 28 379491.0 29 362380.0 30 403607.0 31 482317.0 32 598145.0 33 770436.0 34 1069671.0 35 1775850.0 36 2900931.0 37 5177454.0 38 6684110.0 39 2012024.0 40 3828.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.20688795810936 38.38636997900966 26.075599278248262 18.33114278463272 2 20.371766836085555 36.09680814657993 25.651706018201565 17.87971899913294 3 28.01750260137749 26.43187440206433 25.866657010759987 19.683965985798192 4 18.90379677848936 26.372290228301797 25.9909548466527 28.732958146556143 5 19.55016808728814 35.11824582932071 25.395566332320556 19.936019751070592 6 29.589315830465047 25.929213996883664 25.482698956861395 18.998771215789894 7 20.306361157921643 25.884856213044195 34.64992137595901 19.158861253075155 8 28.582215117614417 26.925746118107334 25.346775350861122 19.145263413417126 9 28.138639257659865 26.672143683984455 25.920283459071243 19.268933599284434 10-11 19.342779333857408 31.448892630556358 30.155038045124844 19.05328999046139 12-13 19.332401992460998 26.536980992814346 29.913657966347092 24.216959048377564 14-15 19.331264504102442 35.65098450983908 25.75473144539221 19.26301954066627 16-17 24.119476103213294 26.323540265713035 25.352317201640577 24.20466642943309 18-19 19.714649034820916 31.082419651832943 25.71963129882154 23.483300014524602 20-21 23.742934920386624 31.53408832746668 25.729559646702633 18.993417105444067 22-23 24.25735121885517 26.7518937671304 29.961128531852673 19.029626482161753 24-25 19.394210230025443 31.272309216415334 25.535588807128374 23.79789174643085 26-27 19.005256737721844 27.295385157770223 34.67546437024331 19.023893734264618 28-29 19.42471306913243 26.9515272896794 34.66160952954847 18.962150111639698 30-31 19.447721874252817 26.528238697095286 35.01365016978959 19.010389258862308 32-33 19.426269056700267 26.606220041146067 34.94739842028996 19.02011248186371 34-35 19.454854131083223 27.085306426320333 34.473970179965505 18.985869262630935 36-37 19.043098584714286 27.069919492481098 34.87331092309064 19.013670999713973 38-39 19.0723811452035 26.687573310944874 34.83144137090955 19.408604172942074 40-41 19.550735007380414 26.746656052308094 34.36703715974026 19.33557178057122 42-43 19.575347070366202 26.801218365670028 34.688646137649144 18.934788426314626 44-45 20.039468752571068 26.87373731987287 34.18248794073821 18.90430598681785 46-47 19.248171376353742 26.983729482828313 34.48164874309912 19.28645039771882 48-49 19.728364909668596 26.992566280478826 33.95410060914699 19.324968200705587 50-51 19.810217665218975 27.06378994906606 33.804825067193214 19.321167318521756 52-53 19.557480497554334 27.63085422848888 33.88367910349352 18.92798617046327 54-55 19.98356860971028 27.566739596458707 32.90892970778529 19.540762086045728 56-57 20.531577320330577 28.64391463669372 31.485213369414065 19.339294673561643 58-59 20.811244830302737 29.25067668747326 30.64317469684626 19.294903785377745 60-61 20.975927517935634 29.995986154518878 29.75514209188247 19.27294423566302 62-63 21.144110350166564 30.188644274171672 29.48643291242636 19.180812463235405 64-65 21.28121973185044 30.236357138238507 29.423897747733037 19.05852538217802 66-67 21.448780130315367 29.92624195241922 29.66926138956793 18.95571652769748 68-69 21.60468373507715 29.520635656507938 30.011982713521157 18.862697894893753 70-71 21.810334275574476 29.221451825664076 30.237094269951857 18.731119628809594 72-73 22.012485753843773 29.058435945358614 30.23747290395819 18.691605396839424 74-75 22.26151903927734 29.10760339610121 30.039310743978337 18.59156682064311 76-77 22.58216677353203 29.239002246164986 29.633738388365327 18.545092591937657 78-79 22.92530871927278 29.371714354960016 29.193093928171436 18.50988299759577 80-81 23.258455423761852 29.543532875568584 28.739172853934118 18.458838846735446 82-83 23.44453566072698 29.711959850244128 28.372110040430364 18.471394448598524 84-85 23.606154872250688 29.746547407702966 28.152102936038226 18.495194784008117 86-87 23.78380220914062 29.696750608097705 28.021441886593802 18.498005296167875 88-89 23.991447544108564 29.634683641639924 27.91033121054662 18.463537603704893 90-91 24.24008291595555 29.556567556101164 27.74246646752613 18.460883060417153 92-93 24.421509747946125 29.527477172857026 27.63328916018157 18.417723919015284 94-95 24.559179985226532 29.548656949334966 27.502554929896565 18.389608135541934 96-97 24.6810289282779 29.523221866465434 27.380650386842543 18.415098818414126 98-99 24.771826609669876 29.543180823886267 27.29241665561138 18.39257591083248 100 24.83389550500586 29.594620996242632 27.165775519330104 18.405707979421408 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 196298.0 1 114443.0 2 25920.5 3 18403.5 4 17185.5 5 17499.0 6 18269.5 7 17811.5 8 17294.5 9 17506.5 10 18239.5 11 26498.0 12 263587.0 13 301985.0 14 101307.0 15 95568.0 16 105055.0 17 114684.5 18 121791.0 19 129069.5 20 139077.0 21 154736.5 22 177256.0 23 201614.5 24 226338.0 25 254476.0 26 286208.0 27 324902.5 28 371922.0 29 418791.5 30 460431.0 31 500679.5 32 543362.0 33 579873.0 34 605380.5 35 634635.5 36 670969.5 37 713341.0 38 762851.0 39 788435.5 40 794522.5 41 813584.5 42 838940.0 43 840812.0 44 828210.5 45 833895.5 46 846008.5 47 848233.5 48 838806.5 49 822004.0 50 803449.5 51 786583.0 52 765859.0 53 721709.0 54 661784.0 55 605913.5 56 554072.5 57 499201.5 58 438659.5 59 371692.5 60 301990.0 61 242267.5 62 189150.0 63 140460.0 64 102913.5 65 74763.0 66 54555.5 67 41618.0 68 31774.0 69 23844.0 70 18030.0 71 14028.5 72 11331.0 73 8996.0 74 7172.0 75 5969.0 76 4529.5 77 2959.0 78 1958.0 79 1451.5 80 1104.5 81 855.0 82 692.0 83 565.5 84 487.0 85 419.5 86 336.5 87 276.5 88 252.5 89 228.5 90 183.5 91 160.5 92 141.5 93 119.0 94 99.0 95 81.5 96 71.5 97 57.5 98 38.0 99 23.5 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0038392007149749582 2 1.1426192604092138E-4 3 0.07375607325941475 4 0.021351745246180175 5 0.01495307738788858 6 0.04742631676871843 7 0.03200857421493011 8 0.022220135884091177 9 0.005774035995934561 10-11 0.008253519791022554 12-13 0.014126582789525914 14-15 0.025295686060025982 16-17 0.042992954038330686 18-19 0.05248621572689723 20-21 0.038451042478204064 22-23 0.02385598579191037 24-25 0.017303064333463528 26-27 0.014920703175510319 28-29 0.011161485808764004 30-31 0.010199781264586249 32-33 0.008249711060154524 34-35 0.006625287344939425 36-37 0.010991997285136638 38-39 0.014635048360408014 40-41 0.01611854903350598 42-43 0.010494957906858629 44-45 0.006798584599434822 46-47 0.008308746388609 48-49 0.00961323671090952 50-51 0.009300920779731001 52-53 0.00796405624505222 54-55 0.005515042296908472 56-57 0.006661470288185717 58-59 0.014038981979561207 60-61 0.024082605278558197 62-63 0.028127477460406815 64-65 0.011949893098446362 66-67 0.00732418945922306 68-69 0.008491565470274475 70-71 0.1791208039926164 72-73 0.14589534026535048 74-75 0.17990730691686474 76-77 0.1146370860314224 78-79 0.11434190938915004 80-81 0.11670332252732907 82-83 0.11521220439249505 84-85 0.11091214724248838 86-87 0.1081241562470899 88-89 0.10541424423448605 90-91 0.10826698365464103 92-93 0.11237469989581217 94-95 0.10937913306810602 96-97 0.10294428226656813 98-99 0.10450967065332875 100 0.10893351155654642 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.6255465E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.706424335280076 #Duplication Level Percentage of deduplicated Percentage of total 1 73.23947649776163 28.34838255416134 2 12.969871759589092 10.040347198016422 3 4.779598707601924 5.550035271863889 4 2.4341364866650785 3.768668789713854 5 1.4016432007378223 2.7126298247209153 6 0.9857345410894394 2.289255565761025 7 0.7099238994765897 1.923503098922832 8 0.5149192724495368 1.5944547086276375 9 0.39103069069389973 1.3621859857904164 >10 2.2280097720962218 15.508377561998998 >50 0.18022545326867945 4.949130147582674 >100 0.1635827265663939 11.109865840224483 >500 8.362804148561192E-4 0.20824266757619234 >1k 8.146747947878518E-4 0.7338636293931091 >5k 1.1762207639670392E-4 0.29206901133722485 >10k+ 7.841471759627149E-5 9.608988144308771 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2002748 7.627928128486775 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 204483 0.7788207140875242 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 184513 0.702760358652951 Illumina Single End PCR Primer 1 (100% over 50bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 62905 0.239588215253472 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 37456 0.14265982339295838 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.8190028247452483 3.808730868030713E-6 0.0 0.0 3.808730868030713E-5 2 0.8190485295156646 3.808730868030713E-6 0.0 0.0 3.808730868030713E-5 3 0.8190561469774007 3.808730868030713E-6 0.0 0.0 4.1896039548337844E-5 4 0.8190713819008728 1.5234923472122851E-5 0.0 0.0 4.1896039548337844E-5 5 0.8190751906317408 1.5234923472122851E-5 0.0 0.0 4.1896039548337844E-5 6 0.8196769701088897 1.5234923472122851E-5 0.0 0.0 4.1896039548337844E-5 7 0.8196769701088897 1.9043654340153564E-5 0.0 0.0 4.1896039548337844E-5 8 0.8196845875706258 1.9043654340153564E-5 0.0 0.0 4.1896039548337844E-5 9 0.8196845875706258 2.2852385208184277E-5 0.0 0.0 4.1896039548337844E-5 10-11 0.8197055355903999 3.0469846944245702E-5 0.0 0.0 4.5704770416368553E-5 12-13 0.8197360054373442 3.9991674114322486E-5 0.0 0.0 4.5704770416368553E-5 14-15 0.8197759971114585 4.38004049823532E-5 0.0 0.0 5.141786671841462E-5 16-17 0.8197969451312327 5.522659758644534E-5 0.0 0.0 5.332223215242998E-5 18-19 0.819836936805347 6.474842475652212E-5 0.0 0.0 5.332223215242998E-5 20-21 0.8198921634029335 9.140954083273711E-5 0.0 0.0 6.284405932250676E-5 22-23 0.8201473483710915 9.521827170076782E-5 0.0 0.0 7.046152105856819E-5 24-25 0.821324246209313 1.0093136800281389E-4 5.713096302046069E-6 1.9043654340153564E-6 7.236588649258354E-5 26-27 0.8250110976895667 1.0854882973887532E-4 7.617461736061426E-6 3.808730868030713E-6 7.998334822864497E-5 28-29 0.8354241678827627 1.1616629147493674E-4 7.617461736061426E-6 3.808730868030713E-6 8.188771366266032E-5 30-31 0.8571491687540098 1.3520994581509032E-4 7.617461736061426E-6 3.808730868030713E-6 8.76008099647064E-5 32-33 0.896965641248403 1.3711431124910567E-4 7.617461736061426E-6 7.617461736061426E-6 9.902700256879854E-5 34-35 0.9629614253642051 1.4282740755115173E-4 1.3330558038107495E-5 7.617461736061426E-6 1.0664446430485996E-4 36-37 1.0623312137111265 1.523492347212285E-4 1.5234923472122851E-5 7.617461736061426E-6 1.1045319517289067E-4 38-39 1.2022144723012904 1.5425360015524386E-4 1.5234923472122851E-5 7.617461736061426E-6 1.1807065690895209E-4 40-41 1.3820608395242666 1.5996669645728994E-4 1.5234923472122851E-5 7.617461736061426E-6 1.2187938777698281E-4 42-43 1.5999583324843036 1.65679792759336E-4 1.5234923472122851E-5 9.521827170076782E-6 1.256881186450135E-4 44-45 1.8595328629677668 1.752016199294128E-4 1.5234923472122851E-5 1.1426192604092138E-5 1.3520994581509032E-4 46-47 2.167341161163971 1.92340908835551E-4 1.5234923472122851E-5 1.1426192604092138E-5 1.3711431124910567E-4 48-49 2.5307607387642914 2.0376710143964313E-4 1.5234923472122851E-5 1.1426192604092138E-5 1.4092304211713638E-4 50-51 2.941159107256337 2.1709765947775062E-4 1.5234923472122851E-5 1.5234923472122851E-5 1.4663613841918243E-4 52-53 3.3856684694024652 2.1709765947775062E-4 1.5234923472122851E-5 1.5234923472122851E-5 1.485405038531978E-4 54-55 3.8644925923041162 2.2852385208184275E-4 1.5234923472122851E-5 1.5234923472122851E-5 1.485405038531978E-4 56-57 4.386791473698905 2.3423694838388883E-4 1.5234923472122851E-5 1.713928890613821E-5 1.485405038531978E-4 58-59 4.950809288656666 2.532806027240424E-4 1.5234923472122851E-5 1.9043654340153564E-5 1.5044486928721316E-4 60-61 5.550886263107509 2.666111607621499E-4 1.5234923472122851E-5 1.9043654340153564E-5 1.523492347212285E-4 62-63 6.171458018359226 2.666111607621499E-4 1.5234923472122851E-5 1.9043654340153564E-5 1.580623310232746E-4 64-65 6.816441834109584 2.72324257064196E-4 1.5234923472122851E-5 1.9043654340153564E-5 1.7139288906138208E-4 66-67 7.487629718231995 2.7803735336624205E-4 1.5234923472122851E-5 2.4756750642199635E-5 1.790103507974435E-4 68-69 8.186095732831241 2.8184608423427275E-4 1.5234923472122851E-5 2.666111607621499E-5 1.8662781253350492E-4 70-71 8.908389167740888 2.8565481510230346E-4 1.5234923472122851E-5 2.666111607621499E-5 1.8662781253350492E-4 72-73 9.642752470771324 2.8946354597033416E-4 1.5234923472122851E-5 2.666111607621499E-5 1.92340908835551E-4 74-75 10.391707021757185 2.8946354597033416E-4 1.5234923472122851E-5 2.666111607621499E-5 1.9424527426956635E-4 76-77 11.157166707959657 2.9517664227238027E-4 1.5234923472122851E-5 2.666111607621499E-5 1.9805400513759708E-4 78-79 11.938642107462199 3.066028348764724E-4 1.5234923472122851E-5 2.666111607621499E-5 2.0567146687365848E-4 80-81 12.738195648029848 3.104115657445031E-4 1.5234923472122851E-5 2.666111607621499E-5 2.1328892860971992E-4 82-83 13.546290267569056 3.2564648921662594E-4 1.5234923472122851E-5 2.666111607621499E-5 2.1328892860971992E-4 84-85 14.36170907656749 3.4659450899079486E-4 1.5234923472122851E-5 2.666111607621499E-5 2.1328892860971992E-4 86-87 15.189313919978183 3.6563816333094843E-4 1.5234923472122851E-5 2.666111607621499E-5 2.19002024911766E-4 88 15.815469274682433 3.7325562506700983E-4 1.5234923472122851E-5 2.666111607621499E-5 2.2090639034578135E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 255285 0.0 87.317795 5 GGTATCA 254820 0.0 87.08764 8 AAGCAGT 263190 0.0 86.551674 1 TGGTATC 256110 0.0 86.461235 7 GTGGTAT 257500 0.0 86.40423 6 GCAGTGG 262895 0.0 86.061676 3 GTATCAA 258340 0.0 85.095604 9 CAGTGGT 264725 0.0 84.638466 4 AGCAGTG 270580 0.0 84.30716 2 CAACGCA 252750 0.0 45.316654 12-13 ATCAACG 254195 0.0 45.125725 10-11 ACGCAGA 256450 0.0 44.954178 14-15 AGAGTAC 257555 0.0 44.46193 18-19 AGTACTT 261425 0.0 43.59005 20-21 TCAACGC 253360 0.0 43.338856 12-13 AACGCAG 254095 0.0 42.973927 14-15 TATCAAC 256340 0.0 42.78232 10-11 CGCAGAG 257830 0.0 42.23604 16-17 GAGTACT 253630 0.0 42.14646 20-21 GCAGAGT 274180 0.0 41.849567 16-17 >>END_MODULE