FastQCFastQC Report
Thu 26 May 2016
SRR1660689_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660689_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26255465
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16672886.350251271497191No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1851790.7052969734110593No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACACAATCTCGTATG1240400.47243497687052965TruSeq Adapter, Index 7 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT724230.27583971565538834No Hit
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACCACACAATCTCGTATG588640.2241971338157599TruSeq Adapter, Index 7 (97% over 35bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT391400.1490737261747221No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2160800.085.147658
AGTGGTA2181800.084.880485
AAGCAGT2224900.084.441711
TGGTATC2183150.084.21187
GTGGTAT2199450.083.9867556
GCAGTGG2242500.083.568453
GTATCAA2194950.083.0552149
CAGTGGT2279800.081.805954
AGCAGTG2319450.081.3578642
CAACGCA2136100.044.6820112-13
ATCAACG2143650.044.45276310-11
ACGCAGA2172850.044.2894114-15
AGAGTAC2198500.043.64033518-19
AGTACTT2232750.042.7238820-21
TCAACGC2139800.042.44034612-13
AACGCAG2150250.042.02456314-15
TATCAAC2173750.041.82583210-11
GAGTACT2152200.041.3575520-21
CGCAGAG2183500.041.32919316-17
GCAGAGT2362300.040.82556516-17
Produced by FastQC (version 0.11.5)