##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660689_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26255465 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.310203761388344 34.0 31.0 34.0 31.0 34.0 2 32.65338854215684 34.0 31.0 34.0 31.0 34.0 3 32.787858451564276 34.0 31.0 34.0 31.0 34.0 4 36.236063272922415 37.0 37.0 37.0 35.0 37.0 5 36.156521089990214 37.0 37.0 37.0 35.0 37.0 6 36.160636728391594 37.0 37.0 37.0 35.0 37.0 7 36.054472011826874 37.0 35.0 37.0 35.0 37.0 8 36.10450239597737 37.0 36.0 37.0 35.0 37.0 9 37.86450580860023 39.0 38.0 39.0 35.0 39.0 10-11 37.732076064925906 39.0 37.5 39.0 35.0 39.0 12-13 37.82636359706446 39.0 38.0 39.0 35.0 39.0 14-15 39.31539249066813 41.0 39.0 41.0 36.0 41.0 16-17 39.25393587582623 41.0 39.0 41.0 36.0 41.0 18-19 39.172900670393766 40.5 39.0 41.0 35.5 41.0 20-21 39.117111713694655 40.5 39.0 41.0 35.5 41.0 22-23 38.934229692751586 40.0 38.5 41.0 34.5 41.0 24-25 38.94128176743394 40.0 39.0 41.0 35.0 41.0 26-27 38.91881692059158 40.0 38.0 41.0 35.0 41.0 28-29 38.829287064616835 40.0 38.0 41.0 35.0 41.0 30-31 38.71133291678514 40.0 38.0 41.0 35.0 41.0 32-33 38.564912124009226 40.0 38.0 41.0 34.0 41.0 34-35 38.467066266013575 40.0 38.0 41.0 34.0 41.0 36-37 38.28178544923885 40.0 38.0 41.0 34.0 41.0 38-39 37.79367712588598 40.0 36.0 41.0 33.5 41.0 40-41 37.68019092406095 40.0 36.0 41.0 33.0 41.0 42-43 37.60837848425081 40.0 36.0 41.0 33.0 41.0 44-45 37.661182671874215 40.0 36.0 41.0 33.0 41.0 46-47 37.5369600386053 40.0 35.5 41.0 33.0 41.0 48-49 37.40402902786144 40.0 35.0 41.0 33.0 41.0 50-51 37.21766470332938 40.0 35.0 41.0 32.5 41.0 52-53 36.82396133528772 39.0 35.0 41.0 31.0 41.0 54-55 36.27076107774134 39.0 35.0 41.0 29.0 41.0 56-57 35.81880299968026 39.0 35.0 41.0 26.5 41.0 58-59 35.5757967531712 39.0 35.0 41.0 26.0 41.0 60-61 35.39912284928109 39.0 35.0 41.0 26.0 41.0 62-63 35.20411409586538 38.0 35.0 40.5 26.0 41.0 64-65 34.915969475307335 37.5 35.0 40.0 25.5 41.0 66-67 34.656674277145726 37.0 34.0 40.0 25.0 41.0 68-69 34.385025479457326 36.5 34.0 39.5 25.0 41.0 70-71 34.00257424501909 36.0 34.0 39.0 24.5 41.0 72-73 32.52843227114812 35.0 33.0 39.0 12.0 40.5 74-75 31.740773187601135 35.0 31.5 37.5 11.0 40.0 76-77 31.15881607886206 34.0 30.0 36.0 19.0 39.0 78-79 32.37094492518034 35.0 33.0 37.0 20.0 39.0 80-81 32.36838585795377 35.0 33.0 36.0 20.0 39.0 82-83 32.12589504699307 35.0 33.0 36.0 19.5 37.5 84-85 31.886841901295597 35.0 33.0 36.0 19.0 37.0 86-87 31.611121646483888 35.0 33.0 35.0 18.5 37.0 88-89 31.421838367745536 35.0 33.0 35.0 18.0 36.0 90-91 31.285834434850038 35.0 33.0 35.0 18.0 36.0 92-93 31.14532439246458 35.0 32.5 35.0 17.0 36.0 94-95 31.014666394215453 35.0 32.0 35.0 17.0 35.5 96-97 30.885979166623024 35.0 32.0 35.0 15.0 35.0 98-99 30.780539632415575 35.0 32.0 35.0 15.0 35.0 100 30.71166014389766 35.0 32.0 35.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5238004138024479 1101 2 0.380049339970455 1101 3 0.32986331000287095 1101 4 0.2529771508640266 1101 5 0.2279424412088389 1101 6 0.21991740427894513 1101 7 0.2590672651555792 1101 8 0.26518152752525026 1101 9 0.3318763960916016 1101 10-11 0.3426273665476458 1101 12-13 0.325614345102224 1101 14-15 0.3611574932466155 1101 16-17 0.4108021523271219 1101 18-19 0.41696618148263553 1101 20-21 0.39021425930138065 1101 22-23 0.37343357369550745 1101 24-25 0.41789029231738084 1101 26-27 0.39861427277967465 1101 28-29 0.38912005117478543 1101 30-31 0.3720770916789604 1101 32-33 0.46298185644337053 1101 34-35 0.42635792182842636 1101 36-37 0.4193579707801618 1101 38-39 0.40560575012113986 1101 40-41 0.41432354709275643 1101 42-43 0.40276617542611604 1101 44-45 0.41070348334565665 1101 46-47 0.332577294022812 1101 48-49 0.3088445182538422 1101 50-51 0.29089440178004367 1101 52-53 0.20783893457029023 1101 54-55 0.18741657317993798 1101 56-57 0.24355832058340354 1101 58-59 0.2828831230769495 1101 60-61 0.2089342735290174 1101 62-63 0.20380996333499013 1101 64-65 0.20900638750920564 1101 66-67 0.20254517958936447 1101 68-69 0.2760892967869353 1101 70-71 0.28829928495635215 1101 72-73 1.3135180956911796 1101 74-75 1.7260000040601007 1101 76-77 0.6078549024332496 1101 78-79 0.41691507997028054 1101 80-81 0.3389231131489865 1101 82-83 0.31019760065245094 1101 84-85 0.27212646362947623 1101 86-87 0.2914923843975892 1101 88-89 0.28438201257368334 1101 90-91 0.22319996742533021 1101 92-93 0.17255208805689293 1101 94-95 0.15001770587380747 1101 96-97 0.16816523999939648 1101 98-99 0.11830163625398171 1101 100 0.12917511086194722 1102 1 0.48034152860626733 1102 2 0.35380042512384335 1102 3 0.2943541722384282 1102 4 0.2091205330062138 1102 5 0.18684509935959426 1102 6 0.20427290800358833 1102 7 0.22410846756607583 1102 8 0.2273727151603282 1102 9 0.27318781452210317 1102 10-11 0.27741437370524125 1102 12-13 0.25931969940945976 1102 14-15 0.32027074114965615 1102 16-17 0.3164640807066732 1102 18-19 0.3282736571950906 1102 20-21 0.3066314864288344 1102 22-23 0.3070534217467298 1102 24-25 0.32478407763034767 1102 26-27 0.32222633629352515 1102 28-29 0.3222653784114158 1102 30-31 0.2941272233954919 1102 32-33 0.35079471949519103 1102 34-35 0.3023869076587218 1102 36-37 0.2649736722092868 1102 38-39 0.3236838567843847 1102 40-41 0.33594324012067034 1102 42-43 0.2999819449683656 1102 44-45 0.317353583301049 1102 46-47 0.23484563972473893 1102 48-49 0.16291997665853586 1102 50-51 0.21331195840831185 1102 52-53 0.14671092594071666 1102 54-55 0.14347624133247905 1102 56-57 0.18881694854700015 1102 58-59 0.2447693337610488 1102 60-61 0.2041398569010937 1102 62-63 0.15552754413916148 1102 64-65 0.12024840891185562 1102 66-67 0.15235921376306294 1102 68-69 0.1551705297625574 1102 70-71 0.19414139021397858 1102 72-73 1.2489324671910538 1102 74-75 1.6351758628665856 1102 76-77 0.4957592885617039 1102 78-79 0.31990443309797456 1102 80-81 0.25437354397632106 1102 82-83 0.22307389277369793 1102 84-85 0.2328654476002896 1102 86-87 0.2590118768815124 1102 88-89 0.23507982164151997 1102 90-91 0.14379278251988836 1102 92-93 0.10652460857545876 1102 94-95 0.12181144960928592 1102 96-97 0.1133964243351322 1102 98-99 0.10549085121082769 1102 100 0.12784508225480096 1103 1 0.451065928359931 1103 2 0.3321584517812113 1103 3 0.2648920176702205 1103 4 0.17064869545959027 1103 5 0.18741929560763282 1103 6 0.1768818642249883 1103 7 0.18903766000150313 1103 8 0.18002576779888813 1103 9 0.2057680200018197 1103 10-11 0.2332593783190191 1103 12-13 0.2243035842053942 1103 14-15 0.2642997014337922 1103 16-17 0.3151493672754242 1103 18-19 0.30824874954868875 1103 20-21 0.29196105124914595 1103 22-23 0.2974704665738557 1103 24-25 0.29525943962374157 1103 26-27 0.25421245853762997 1103 28-29 0.29471798476789957 1103 30-31 0.277511537393508 1103 32-33 0.30833359268742555 1103 34-35 0.30602274691117515 1103 36-37 0.3051568579446595 1103 38-39 0.3085551732770284 1103 40-41 0.29580463053624584 1103 42-43 0.255732179421571 1103 44-45 0.23155848609516028 1103 46-47 0.19935426780164534 1103 48-49 0.17322601101091806 1103 50-51 0.1893629716228915 1103 52-53 0.1204296666354665 1103 54-55 0.07584112684765643 1103 56-57 0.1238511742335433 1103 58-59 0.19099343411168235 1103 60-61 0.13550613561813662 1103 62-63 0.14197434391715547 1103 64-65 0.07066125476212193 1103 66-67 0.13975224633718142 1103 68-69 0.1697094890514279 1103 70-71 0.17766714730268518 1103 72-73 1.200546055349534 1103 74-75 1.558922748975366 1103 76-77 0.4132436052438244 1103 78-79 0.29052682991569156 1103 80-81 0.1943343056619753 1103 82-83 0.16882004649018967 1103 84-85 0.16527358462836617 1103 86-87 0.17483669590779627 1103 88-89 0.10251676098410556 1103 90-91 0.04897403896685759 1103 92-93 0.07192413653986662 1103 94-95 0.08631060299236992 1103 96-97 0.11594416099120508 1103 98-99 0.07671298008589744 1103 100 0.05280205474024058 1104 1 0.4004333225012431 1104 2 0.2821462425296133 1104 3 0.21724566243176469 1104 4 0.14439466247234378 1104 5 0.1492423970653789 1104 6 0.14437303155341397 1104 7 0.1583257754145322 1104 8 0.15947290442581163 1104 9 0.18216931092075583 1104 10-11 0.19852415281578573 1104 12-13 0.19692763416760783 1104 14-15 0.20597530700060673 1104 16-17 0.246041896692752 1104 18-19 0.2613352661405699 1104 20-21 0.22907768251869243 1104 22-23 0.23856696990337412 1104 24-25 0.25318754206100635 1104 26-27 0.23215265333422508 1104 28-29 0.24806764235978562 1104 30-31 0.22110900331446715 1104 32-33 0.23412692313802097 1104 34-35 0.25312962711532094 1104 36-37 0.2349783964947747 1104 38-39 0.24981612078646975 1104 40-41 0.2685286778357181 1104 42-43 0.2260180900904345 1104 44-45 0.22211985973316217 1104 46-47 0.20406062164211392 1104 48-49 0.17186488955526613 1104 50-51 0.16690818129358576 1104 52-53 0.08329328075509324 1104 54-55 0.10276057539135053 1104 56-57 0.16405252061097286 1104 58-59 0.22279643627162926 1104 60-61 0.199513865427555 1104 62-63 0.16332465665023932 1104 64-65 0.15125795146302323 1104 66-67 0.1515158343865579 1104 68-69 0.17905558538623012 1104 70-71 0.1733631859999747 1104 72-73 1.221385027055561 1104 74-75 1.6066324075452236 1104 76-77 0.44623639910811264 1104 78-79 0.2637188283426539 1104 80-81 0.18962219161041816 1104 82-83 0.20059682684783553 1104 84-85 0.17677488023569765 1104 86-87 0.24465900714480782 1104 88-89 0.23960127320041025 1104 90-91 0.13549451516052358 1104 92-93 0.10102905110260352 1104 94-95 0.09441856523043057 1104 96-97 0.1171852337303747 1104 98-99 0.14509342163625405 1104 100 0.12544398159519687 1105 1 0.33856315335025045 1105 2 0.23160167775253626 1105 3 0.1696241556386937 1105 4 0.1330843555613228 1105 5 0.12285904632224032 1105 6 0.12874361151666847 1105 7 0.09998012415697843 1105 8 0.12426737126139642 1105 9 0.13426222053522707 1105 10-11 0.14697486161564655 1105 12-13 0.1374149136181444 1105 14-15 0.13841626531478113 1105 16-17 0.173592687810725 1105 18-19 0.19429044864471479 1105 20-21 0.16223561760620697 1105 22-23 0.14362237333472194 1105 24-25 0.18784606776716117 1105 26-27 0.15371679982106912 1105 28-29 0.19676383821267507 1105 30-31 0.16454230298195682 1105 32-33 0.19920667474132614 1105 34-35 0.17605770255235598 1105 36-37 0.13655546142819475 1105 38-39 0.1399327371059229 1105 40-41 0.16698676465207996 1105 42-43 0.1470135503129626 1105 44-45 0.10188408222176548 1105 46-47 0.09355492616335681 1105 48-49 0.09528117370334144 1105 50-51 0.05777884553638302 1105 52-53 0.04627889562099341 1105 54-55 0.022842644886956975 1105 56-57 0.0414442637826653 1105 58-59 0.08465118235204017 1105 60-61 0.04353053564063458 1105 62-63 0.025310057515426365 1105 64-65 0.011717870622021564 1105 66-67 -0.01997721418275944 1105 68-69 -0.008397897457683712 1105 70-71 0.018736089160100278 1105 72-73 1.0614964580905308 1105 74-75 1.4607017191458773 1105 76-77 0.3123225161121823 1105 78-79 0.12733121256015778 1105 80-81 0.08408415432204919 1105 82-83 0.05774952687713153 1105 84-85 0.0776722632422846 1105 86-87 0.09288884192023872 1105 88-89 0.09659689440337615 1105 90-91 0.032862432782774675 1105 92-93 -0.016782422947766662 1105 94-95 -0.004652982431821329 1105 96-97 0.008257899274017433 1105 98-99 -0.01879822121896524 1105 100 0.0055510134573388825 1106 1 0.31011002237268315 1106 2 0.20651518475241204 1106 3 0.13093936728402156 1106 4 0.08873417593044053 1106 5 0.10612328680629446 1106 6 0.09704460778077362 1106 7 0.10268756334010476 1106 8 0.11942793633797066 1106 9 0.10966543881952617 1106 10-11 0.13404583382358481 1106 12-13 0.11788455778602724 1106 14-15 0.1233772621799929 1106 16-17 0.11936029800454406 1106 18-19 0.1486815245740658 1106 20-21 0.10566909486777121 1106 22-23 0.110028559110944 1106 24-25 0.15025603650763486 1106 26-27 0.1252919172881235 1106 28-29 0.13602302950238254 1106 30-31 0.1474286488757457 1106 32-33 0.19393102310773003 1106 34-35 0.173098301011386 1106 36-37 0.15104479539828475 1106 38-39 0.1557749475246908 1106 40-41 0.1441409909835727 1106 42-43 0.1594264467644919 1106 44-45 0.1439064248609654 1106 46-47 0.11682509517348194 1106 48-49 0.08184240050971425 1106 50-51 0.04375109440396585 1106 52-53 -0.017959227162101854 1106 54-55 -0.037455211443557346 1106 56-57 -0.011240928033252828 1106 58-59 0.004783803518044749 1106 60-61 0.013919366852043424 1106 62-63 -0.016915610452485907 1106 64-65 -0.016317873047107412 1106 66-67 0.002263347099031421 1106 68-69 0.029484219656112032 1106 70-71 0.0370607917128325 1106 72-73 1.1386416972135578 1106 74-75 1.512527034686375 1106 76-77 0.30474429414248405 1106 78-79 0.19254189987627512 1106 80-81 0.08019255252968804 1106 82-83 0.03942194437565405 1106 84-85 0.020413898343129233 1106 86-87 0.08802053487908168 1106 88-89 0.09984486712432528 1106 90-91 0.015179360389666385 1106 92-93 -0.0013363381105868655 1106 94-95 -7.573054488290154E-4 1106 96-97 0.0714417318733318 1106 98-99 0.0703447012189713 1106 100 0.0711323015461538 1107 1 0.2884234494360882 1107 2 0.19952387391787596 1107 3 0.10841550928446964 1107 4 0.08861401173565042 1107 5 0.10260491441619735 1107 6 0.10378145203007705 1107 7 0.10879326591342675 1107 8 0.10747016180750535 1107 9 0.12171265904709827 1107 10-11 0.11031610094164535 1107 12-13 0.10849484876389681 1107 14-15 0.10191674933122385 1107 16-17 0.14146016940215134 1107 18-19 0.12664052992313657 1107 20-21 0.11454208454259884 1107 22-23 0.10362431314788978 1107 24-25 0.13906981953459763 1107 26-27 0.10231496256947992 1107 28-29 0.10842395855262055 1107 30-31 0.12604104927719817 1107 32-33 0.1270638285527781 1107 34-35 0.10175465222378932 1107 36-37 0.12880818922444348 1107 38-39 0.13898091783318023 1107 40-41 0.17633150826962662 1107 42-43 0.15786359365776548 1107 44-45 0.18156738974764863 1107 46-47 0.1605955536559165 1107 48-49 0.14780190690184725 1107 50-51 0.09643363121768544 1107 52-53 0.08359408184004735 1107 54-55 0.08061477320818966 1107 56-57 0.07400161765580293 1107 58-59 0.14283765217260935 1107 60-61 0.07163453304062983 1107 62-63 0.06846469889778461 1107 64-65 0.0648263605584205 1107 66-67 0.07753076914822543 1107 68-69 0.12581861128288097 1107 70-71 0.12478926633424692 1107 72-73 1.2095439236870078 1107 74-75 1.558490899483143 1107 76-77 0.4059085747044975 1107 78-79 0.2938395175013184 1107 80-81 0.21302160735024955 1107 82-83 0.21736253148718987 1107 84-85 0.13155417889179688 1107 86-87 0.18468614671431993 1107 88-89 0.19257320606900663 1107 90-91 0.14280135468744604 1107 92-93 0.11728826340117493 1107 94-95 0.1787612913817611 1107 96-97 0.16540238639941052 1107 98-99 0.1576298954018327 1107 100 0.19276272472990996 1108 1 0.2590203179317285 1108 2 0.13793006564441868 1108 3 0.09951993082593447 1108 4 0.06968826529769956 1108 5 0.043251051792275064 1108 6 0.0431188961571749 1108 7 0.05584221534946465 1108 8 0.03967256922206275 1108 9 0.03145843980715313 1108 10-11 0.07114636173909616 1108 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-0.7524866300283719 2316 100 -0.7706858157641605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 22.0 7 388.0 8 772.0 9 1122.0 10 4082.0 11 5516.0 12 4131.0 13 4494.0 14 8269.0 15 17570.0 16 30103.0 17 43297.0 18 56053.0 19 72505.0 20 104783.0 21 120113.0 22 152978.0 23 239006.0 24 377654.0 25 497160.0 26 504860.0 27 430555.0 28 387682.0 29 413877.0 30 457902.0 31 504713.0 32 608988.0 33 774722.0 34 1080230.0 35 1783207.0 36 2958427.0 37 5566461.0 38 7041272.0 39 1998854.0 40 3696.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.84981894723598 36.89848421394501 26.466509447232795 19.785187391586216 2 20.511921260977342 34.232511931620735 27.685256436722227 17.570310370679696 3 26.164537554372014 27.34920139483342 26.341228388070824 20.145032662723743 4 18.862659418238906 27.10786597317783 26.96333390503237 27.06614070355089 5 19.947281475155844 34.031907554467026 25.957556662778053 20.063254307599077 6 28.071294871372494 26.50041429470017 26.280894282390356 19.147396551536985 7 20.483929726630244 26.704390876337552 33.57940527810115 19.232274118931052 8 26.808917686279788 27.288760644688637 26.444422142209252 19.45789952682232 9 26.48603633567335 26.930930379637154 27.099820932518238 19.483212352171254 10-11 19.54342457846395 31.055357427491764 30.091160068960882 19.310057925083406 12-13 19.565259121761205 27.039972187114174 29.693416865431704 23.701351825692914 14-15 19.163514781975785 34.96166415702947 25.900688066537032 19.974132994457726 16-17 23.14612451344054 27.256726172636057 25.91555944002008 23.68158987390332 18-19 19.896549087575064 30.79413329367172 26.28387689830844 23.025440720444777 20-21 23.15657795657085 30.82297701509701 26.3055266340244 19.714918394307738 22-23 23.26908175629496 27.6339712062607 29.764933315119986 19.332013722324355 24-25 19.38488374858911 31.02176579817649 26.016991426584973 23.576359026649428 26-27 19.25069622797864 26.986251527306997 34.01807458292754 19.744977661786823 28-29 19.278688389659138 27.503382246088808 33.55143346112909 19.66649590312296 30-31 19.70307272658803 27.09607067458682 33.90103359326771 19.299823005557435 32-33 19.332478953822626 27.566295141878005 33.385898072484984 19.715327831814385 34-35 19.33222469854048 27.200055212551455 33.35310791982005 20.11461216908802 36-37 19.318982213500515 27.629589564489155 33.31511912689531 19.736309095115022 38-39 19.388553827058793 27.65765128719842 33.28158643639046 19.672208449352315 40-41 19.39657899822559 28.14540824672054 33.24359627959145 19.214416475462418 42-43 19.42897629102821 27.369744143958513 33.57398696610359 19.627292598909687 44-45 19.46568579356514 27.428292467325132 33.4797244842653 19.626297254844435 46-47 19.891314081672274 27.5743102594728 33.34619907922728 19.188176579627637 48-49 19.518296953437062 28.031703561255895 33.213573177710515 19.236426307596524 50-51 19.964758119454086 27.718223504629485 32.64038816821057 19.67663020770586 52-53 20.04311970963444 27.862905228918265 32.36208915037158 19.731885911075718 54-55 19.95256647963034 28.147533563053894 31.94366253733676 19.956237419978997 56-57 20.345600638157848 28.70739766740458 31.162376143726238 19.784625550711333 58-59 20.56017561147481 29.236654457701718 30.021040957738798 20.18212897308467 60-61 20.97040588085926 29.557080164408077 29.19901760273143 20.273496352001235 62-63 20.56802462705014 29.74794882141666 29.769129914099075 19.914896637434133 64-65 20.969638318778227 30.286655357245557 28.806461833686352 19.937244490289867 66-67 20.476709157569665 30.60391228335834 28.908516841111858 20.01086171796013 68-69 20.415949277625415 30.40517503323797 29.106408871321655 20.07246681781496 70-71 20.329117682204647 30.280808989372527 29.22561660086239 20.164456727560438 72-73 20.76290969951572 30.011243113398322 29.008490328597354 20.217356858488607 74-75 20.252426637738505 30.142999657038587 29.048716162867954 20.555857542354953 76-77 20.253897167916186 30.250334448361993 28.78851988536193 20.70724849835989 78-79 20.336832461872422 30.378044570381483 28.384803877883126 20.90031908986297 80-81 20.331347636028713 30.554501628552337 28.016463896637255 21.097686838781687 82-83 20.295959426676376 30.723761727549743 27.630165918311754 21.350112927462128 84-85 20.245008199646765 30.74396827710254 27.40954663049412 21.601476892756576 86-87 20.183039278346552 30.75974816370885 27.22456942634729 21.832643131597308 88-89 20.143732531031148 30.768796472349237 27.090720740323786 21.996750256295826 90-91 20.12780313648353 30.713958794496772 26.933780250416188 22.22445781860351 92-93 20.11394900776742 30.685629039852678 26.819897082212137 22.380524870167765 94-95 20.092951623152825 30.710408031329724 26.7071495391123 22.48949080640515 96-97 20.059338666632005 30.644316106621876 26.64865090126299 22.64769432548313 98-99 20.00935379451831 30.598633291610117 26.628137331763497 22.763875582108074 100 19.909826012466496 30.608882390859282 26.576254629307677 22.90503696736654 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 186865.0 1 113786.5 2 30924.0 3 19968.5 4 18375.0 5 18515.0 6 19575.0 7 19524.5 8 19070.0 9 19573.5 10 20721.0 11 28661.0 12 235839.5 13 272220.5 14 95788.5 15 86128.5 16 94401.5 17 103314.5 18 110410.0 19 118714.0 20 130662.0 21 148314.0 22 173287.0 23 201308.0 24 228918.5 25 258862.5 26 293455.0 27 334466.0 28 384202.5 29 433314.5 30 474931.0 31 515463.5 32 559307.5 33 596777.5 34 621728.0 35 648367.0 36 682541.0 37 723041.0 38 770231.5 39 797623.5 40 805548.5 41 823866.0 42 849055.5 43 850484.5 44 838097.5 45 842131.0 46 846503.0 47 839964.0 48 826626.5 49 813286.0 50 801909.0 51 788970.0 52 768775.5 53 724927.0 54 665164.5 55 607479.0 56 552453.0 57 493827.0 58 430604.0 59 363398.0 60 293145.5 61 233496.0 62 181412.0 63 133042.5 64 96259.0 65 69246.5 66 50269.5 67 38100.5 68 28581.5 69 20960.5 70 15489.0 71 11772.5 72 9358.0 73 7273.0 74 5599.0 75 4536.5 76 3275.0 77 1898.0 78 1080.5 79 641.0 80 386.0 81 266.5 82 192.5 83 121.5 84 80.0 85 55.0 86 36.0 87 25.0 88 15.5 89 9.5 90 7.0 91 5.0 92 3.5 93 2.5 94 1.5 95 1.5 96 1.5 97 0.5 98 0.0 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22455515451735475 2 7.998334822864497E-5 3 0.0 4 0.0014930225002680395 5 6.779540945094668E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.427857781227641E-5 14-15 3.808730868030713E-5 16-17 0.0017215463523498824 18-19 6.665279019053747E-5 20-21 1.352099458150903E-4 22-23 1.9043654340153562E-6 24-25 0.004663790947903607 26-27 0.026883926831994785 28-29 0.04779576366291742 30-31 0.07470063851468636 32-33 0.07934157707738179 34-35 0.08493279399165089 36-37 0.09182469249735246 38-39 0.08504896028312582 40-41 0.050425692327292625 42-43 0.03623816984387822 44-45 0.03862053100183143 46-47 0.07286673460172959 48-49 0.09992205432278575 50-51 0.11477039161180348 52-53 0.40614020738158707 54-55 0.396593623460868 56-57 0.40065944366249084 58-59 0.3973515609036061 60-61 0.41908989233289146 62-63 0.40808456448971675 64-65 0.3954110125263445 66-67 0.39605087931217364 68-69 0.4057498124676139 70-71 0.41172571119955403 72-73 0.9784001159377677 74-75 2.60545756854811 76-77 0.41250269229663233 78-79 0.42078477756916516 80-81 0.44353623140934656 82-83 0.43552075729757594 84-85 0.43455714838796416 86-87 0.41286452172909527 88-89 0.39441883813522255 90-91 0.3728404734023945 92-93 0.36246549051787885 94-95 0.3637833113982175 96-97 0.3796162056166211 98-99 0.373887874391103 100 0.37506858096019247 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.6255465E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.035505356566745 #Duplication Level Percentage of deduplicated Percentage of total 1 71.74063413029963 27.286912737465062 2 13.695096178754055 10.417998081313932 3 4.998926384728787 5.704100738503038 4 2.524205373359847 3.8403770799801213 5 1.5786881527204935 3.0023100844574393 6 0.9848204403764432 2.247488588111678 7 0.7711054445072361 2.053056968552293 8 0.5505291716732575 1.6751724206499556 9 0.43547439680601513 1.4907139877126576 >10 2.353349891256248 16.11388826965871 >50 0.1941637610951678 5.230791038662439 >100 0.17114793488556532 11.59331874125238 >500 9.088399606826409E-4 0.22194165328117693 >1k 8.100802514049976E-4 0.7100042161008487 >5k 5.992256791210842E-5 0.16107761203082632 >10k+ 7.989675721373146E-5 8.250847782267561 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1667288 6.350251271497191 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 185179 0.7052969734110593 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACACAATCTCGTATG 124040 0.47243497687052965 TruSeq Adapter, Index 7 (97% over 37bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 72423 0.27583971565538834 No Hit AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACCACACAATCTCGTATG 58864 0.2241971338157599 TruSeq Adapter, Index 7 (97% over 35bp) AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 39140 0.1490737261747221 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.8179401888330677 7.617461736061426E-6 3.808730868030713E-6 3.808730868030713E-6 2.2852385208184277E-5 2 0.8179935110652201 7.617461736061426E-6 3.808730868030713E-6 3.808730868030713E-6 2.2852385208184277E-5 3 0.8180011285269562 7.617461736061426E-6 3.808730868030713E-6 7.617461736061426E-6 2.2852385208184277E-5 4 0.8180163634504283 1.1426192604092138E-5 3.808730868030713E-6 7.617461736061426E-6 2.666111607621499E-5 5 0.8180201721812963 1.9043654340153564E-5 3.808730868030713E-6 1.1426192604092138E-5 3.0469846944245702E-5 6 0.8187095524684099 2.2852385208184277E-5 3.808730868030713E-6 1.1426192604092138E-5 3.0469846944245702E-5 7 0.818713361199278 2.2852385208184277E-5 3.808730868030713E-6 1.1426192604092138E-5 3.0469846944245702E-5 8 0.8187285961227501 2.2852385208184277E-5 3.808730868030713E-6 1.1426192604092138E-5 3.427857781227642E-5 9 0.8187362135844861 2.666111607621499E-5 3.808730868030713E-6 1.1426192604092138E-5 3.808730868030713E-5 10-11 0.8187552572388263 3.427857781227642E-5 3.808730868030713E-6 1.1426192604092138E-5 3.808730868030713E-5 12-13 0.8187971532783747 4.5704770416368553E-5 3.808730868030713E-6 1.1426192604092138E-5 4.189603954833784E-5 14-15 0.8188904671846413 4.951350128439927E-5 3.808730868030713E-6 1.1426192604092138E-5 6.284405932250676E-5 16-17 0.8189228413970197 4.951350128439927E-5 3.808730868030713E-6 1.1426192604092138E-5 6.665279019053749E-5 18-19 0.8189628330711339 6.284405932250676E-5 3.808730868030713E-6 1.5234923472122851E-5 7.427025192659889E-5 20-21 0.8190199640341544 7.046152105856819E-5 3.808730868030713E-6 1.9043654340153564E-5 7.617461736061426E-5 22-23 0.8192808620986145 7.236588649258354E-5 3.808730868030713E-6 1.9043654340153564E-5 7.807898279462962E-5 24-25 0.8204444293787979 7.807898279462962E-5 3.808730868030713E-6 2.2852385208184277E-5 8.379207909667569E-5 26-27 0.8241408026862217 8.950517539872176E-5 3.808730868030713E-6 2.2852385208184277E-5 8.950517539872176E-5 28-29 0.8345710121683239 9.331390626675246E-5 3.808730868030713E-6 2.2852385208184277E-5 1.0093136800281389E-4 30-31 0.8563417178099874 9.902700256879854E-5 3.808730868030713E-6 2.4756750642199635E-5 1.0854882973887532E-4 32-33 0.8961162942648321 1.0093136800281389E-4 3.808730868030713E-6 2.666111607621499E-5 1.1045319517289067E-4 34-35 0.9622701407116576 1.0664446430485996E-4 3.808730868030713E-6 2.666111607621499E-5 1.180706569089521E-4 36-37 1.0613447524163064 1.1045319517289067E-4 3.808730868030713E-6 2.666111607621499E-5 1.2187938777698281E-4 38-39 1.2007595371097026 1.180706569089521E-4 3.808730868030713E-6 2.666111607621499E-5 1.2378375321099816E-4 40-41 1.3797279918675978 1.2187938777698281E-4 1.1426192604092138E-5 2.666111607621499E-5 1.2759248407902886E-4 42-43 1.595875372993775 1.2187938777698281E-4 1.1426192604092138E-5 2.666111607621499E-5 1.314012149470596E-4 44-45 1.8539302198608936 1.3330558038107497E-4 1.1426192604092138E-5 2.666111607621499E-5 1.3901867668312103E-4 46-47 2.1593371132448045 1.4473177298516708E-4 1.1426192604092138E-5 2.666111607621499E-5 1.4092304211713638E-4 48-49 2.5199458474645184 1.5425360015524386E-4 1.1426192604092138E-5 2.666111607621499E-5 1.4092304211713638E-4 50-51 2.927264857049761 1.65679792759336E-4 1.1426192604092138E-5 2.666111607621499E-5 1.4473177298516708E-4 52-53 3.368647251153236 1.7710598536342816E-4 1.1426192604092138E-5 2.666111607621499E-5 1.4473177298516708E-4 54-55 3.84361693841644 1.8662781253350492E-4 1.1426192604092138E-5 2.666111607621499E-5 1.5044486928721313E-4 56-57 4.361274881248533 1.92340908835551E-4 1.1426192604092138E-5 3.0469846944245702E-5 1.580623310232746E-4 58-59 4.920847907283303 1.9995837057161243E-4 1.1426192604092138E-5 3.0469846944245702E-5 1.5996669645728994E-4 60-61 5.513665440699679 2.1138456317570456E-4 1.1426192604092138E-5 3.0469846944245702E-5 1.5996669645728994E-4 62-63 6.105180388159189 2.228107557797967E-4 1.1426192604092138E-5 3.237421237826106E-5 1.6377542732532065E-4 64-65 6.695070911903484 2.266194866478274E-4 1.1426192604092138E-5 3.427857781227642E-5 1.7329725449539743E-4 66-67 7.311837745018037 2.3423694838388883E-4 1.1426192604092138E-5 3.808730868030713E-5 1.790103507974435E-4 68-69 7.950706643359773 2.3614131381790419E-4 1.1426192604092138E-5 3.808730868030713E-5 1.8662781253350492E-4 70-71 8.61584626286375 2.4375877555396562E-4 1.1426192604092138E-5 3.808730868030713E-5 1.92340908835551E-4 72-73 9.299429661596168 2.532806027240424E-4 1.1426192604092138E-5 3.808730868030713E-5 1.9995837057161243E-4 74-75 10.023777906809116 2.5708933359207313E-4 1.1426192604092138E-5 3.808730868030713E-5 2.0567146687365848E-4 76-77 10.788091165020312 2.7041989163018065E-4 1.1426192604092138E-5 3.808730868030713E-5 2.0567146687365848E-4 78-79 11.56855915520826 2.7803735336624205E-4 1.1426192604092138E-5 3.808730868030713E-5 2.1138456317570456E-4 80-81 12.366642525660849 2.7803735336624205E-4 1.1426192604092138E-5 3.808730868030713E-5 2.1709765947775062E-4 82-83 13.17238715825448 2.875591805363188E-4 1.1426192604092138E-5 3.808730868030713E-5 2.2090639034578135E-4 84-85 13.986848071439603 2.9517664227238027E-4 1.1426192604092138E-5 3.808730868030713E-5 2.228107557797967E-4 86-87 14.813279825742946 3.04698469442457E-4 1.1426192604092138E-5 4.1896039548337844E-5 2.3233258294987348E-4 88 15.439159047459261 3.04698469442457E-4 1.1426192604092138E-5 4.1896039548337844E-5 2.3614131381790419E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 216080 0.0 85.14765 8 AGTGGTA 218180 0.0 84.88048 5 AAGCAGT 222490 0.0 84.44171 1 TGGTATC 218315 0.0 84.2118 7 GTGGTAT 219945 0.0 83.986755 6 GCAGTGG 224250 0.0 83.56845 3 GTATCAA 219495 0.0 83.055214 9 CAGTGGT 227980 0.0 81.80595 4 AGCAGTG 231945 0.0 81.357864 2 CAACGCA 213610 0.0 44.68201 12-13 ATCAACG 214365 0.0 44.452763 10-11 ACGCAGA 217285 0.0 44.28941 14-15 AGAGTAC 219850 0.0 43.640335 18-19 AGTACTT 223275 0.0 42.72388 20-21 TCAACGC 213980 0.0 42.440346 12-13 AACGCAG 215025 0.0 42.024563 14-15 TATCAAC 217375 0.0 41.825832 10-11 GAGTACT 215220 0.0 41.35755 20-21 CGCAGAG 218350 0.0 41.329193 16-17 GCAGAGT 236230 0.0 40.825565 16-17 >>END_MODULE