FastQCFastQC Report
Thu 26 May 2016
SRR1660688_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660688_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36717861
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24516646.677033828304976No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1928370.5251858216904302No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT774920.21104715222926518No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT542240.1476774477685397No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3163450.086.591778
GTGGTAT3178850.086.325546
AGTGGTA3192200.086.2295155
TGGTATC3180700.085.91737
AAGCAGT3300450.085.193461
GCAGTGG3296600.084.801973
GTATCAA3212750.084.406049
AGCAGTG3409650.082.6320652
CAGTGGT3366800.082.2657554
CAACGCA3126300.045.489112-13
ATCAACG3138700.045.32430310-11
ACGCAGA3192150.044.89637414-15
AGAGTAC3198300.044.4388618-19
TCAACGC3124850.043.3660612-13
AACGCAG3145650.042.83814214-15
AGTACTT3293750.042.8130820-21
TATCAAC3175200.042.6120910-11
CGCAGAG3206350.041.94267716-17
GCAGAGT3411200.041.77472716-17
GAGTACT3162300.041.5914420-21
Produced by FastQC (version 0.11.5)