##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660688_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 36717861 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19222824553968 34.0 31.0 34.0 30.0 34.0 2 32.337857316906344 34.0 31.0 34.0 30.0 34.0 3 32.31580461617849 34.0 31.0 34.0 30.0 34.0 4 35.71367580480791 37.0 35.0 37.0 35.0 37.0 5 35.73842362440448 37.0 35.0 37.0 35.0 37.0 6 35.749205761196166 37.0 35.0 37.0 35.0 37.0 7 35.67488487414885 37.0 35.0 37.0 35.0 37.0 8 35.74343826836754 37.0 35.0 37.0 35.0 37.0 9 37.425018875691045 39.0 38.0 39.0 35.0 39.0 10-11 37.32417678143071 39.0 37.0 39.0 34.5 39.0 12-13 37.39666002058236 39.0 38.0 39.0 35.0 39.0 14-15 38.82094719515388 41.0 39.0 41.0 35.0 41.0 16-17 38.751498242231484 40.5 38.5 41.0 35.0 41.0 18-19 38.62815446956455 40.0 38.0 41.0 34.0 41.0 20-21 38.549716036018545 40.0 38.0 41.0 34.0 41.0 22-23 38.349122393050074 40.0 38.0 41.0 34.0 41.0 24-25 38.36883068978337 40.0 38.0 41.0 34.0 41.0 26-27 38.369215516121706 40.0 38.0 41.0 34.0 41.0 28-29 38.29252866881325 40.0 38.0 41.0 34.0 41.0 30-31 38.187741015196934 40.0 38.0 41.0 33.5 41.0 32-33 38.06216002070491 40.0 38.0 41.0 33.0 41.0 34-35 37.944163441329 40.0 38.0 41.0 33.0 41.0 36-37 37.74085787568072 40.0 37.5 41.0 33.0 41.0 38-39 37.353558966847224 40.0 35.5 41.0 32.5 41.0 40-41 37.39158656328047 40.0 35.0 41.0 32.5 41.0 42-43 37.26688612117138 40.0 35.0 41.0 32.0 41.0 44-45 37.13204802425719 40.0 35.0 41.0 31.5 41.0 46-47 37.22202315924667 40.0 35.0 41.0 32.0 41.0 48-49 37.1664572590435 40.0 35.0 41.0 31.5 41.0 50-51 35.77940617238025 38.5 34.0 40.0 29.0 40.5 52-53 35.88778981433586 38.5 35.0 39.5 29.5 40.5 54-55 36.202227643380425 39.0 35.0 41.0 27.5 41.0 56-57 35.76769832806982 39.5 35.0 41.0 24.5 41.0 58-59 35.554352621466705 39.0 35.0 41.0 24.5 41.0 60-61 35.39557074144379 39.0 35.0 41.0 24.5 41.0 62-63 35.184831559223994 39.0 35.0 41.0 24.0 41.0 64-65 34.92757829765738 38.0 35.0 41.0 23.0 41.0 66-67 34.65490041753794 37.0 34.0 40.0 23.0 41.0 68-69 34.408737072129554 37.0 34.0 40.0 23.0 41.0 70-71 34.098638248562466 36.0 34.0 39.5 23.0 41.0 72-73 33.78124155979565 36.0 34.0 39.0 23.0 41.0 74-75 33.39526854519113 35.0 33.0 39.0 22.0 40.5 76-77 32.99796043402419 35.0 33.0 37.5 20.5 40.0 78-79 32.60269485741558 35.0 33.0 37.0 20.0 39.0 80-81 32.198698325591465 35.0 33.0 36.5 19.0 39.0 82-83 31.892393881549907 35.0 32.0 36.0 18.5 38.5 84-85 31.584525906887656 35.0 32.0 36.0 18.0 37.0 86-87 31.3130451144744 35.0 32.0 35.0 17.0 37.0 88-89 31.129952667994466 35.0 32.0 35.0 17.0 36.5 90-91 30.921932394155533 35.0 31.5 35.0 15.5 36.0 92-93 30.7553007104635 35.0 31.0 35.0 15.0 36.0 94-95 30.59560717602804 35.0 31.0 35.0 15.0 36.0 96-97 30.4513962155911 35.0 31.0 35.0 13.0 35.0 98-99 30.321736538520042 35.0 31.0 35.0 11.0 35.0 100 30.213468807455858 34.0 31.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3445397325286237 1101 2 0.3403972251282781 1101 3 0.31831394742967944 1101 4 0.28587814768258113 1101 5 0.3168518807950207 1101 6 0.21288139956013197 1101 7 0.26247073781844676 1101 8 0.26655988035479083 1101 9 0.34533026615873297 1101 10-11 0.3406567047422442 1101 12-13 0.3602021602793144 1101 14-15 0.40965843508843136 1101 16-17 0.39682472461321083 1101 18-19 0.4616229696315557 1101 20-21 0.4145225279929363 1101 22-23 0.39544599103076905 1101 24-25 0.39985296533887293 1101 26-27 0.40506840321549475 1101 28-29 0.3963468236005241 1101 30-31 0.40309380403054007 1101 32-33 0.38010318063764004 1101 34-35 0.38975494776942554 1101 36-37 0.34809670621520183 1101 38-39 0.38333038325449564 1101 40-41 0.38088797850086564 1101 42-43 0.351986934734839 1101 44-45 0.34982984113435833 1101 46-47 0.3649813400326778 1101 48-49 0.384100482801891 1101 50-51 0.33375515133663924 1101 52-53 0.4006052208736932 1101 54-55 0.4544359100269588 1101 56-57 0.4485438398944481 1101 58-59 0.41599211628663113 1101 60-61 0.4113196043515899 1101 62-63 0.38676897119961495 1101 64-65 0.3723558854959208 1101 66-67 0.363689154730352 1101 68-69 0.365790343228376 1101 70-71 0.3265548407533245 1101 72-73 0.28213628732373763 1101 74-75 0.30998351085582243 1101 76-77 0.2673204364453454 1101 78-79 0.27704311708378526 1101 80-81 0.34066913322411096 1101 82-83 0.26786353967094456 1101 84-85 0.2571245367430457 1101 86-87 0.29554039210849936 1101 88-89 0.2581728067030902 1101 90-91 0.22728339854372948 1101 92-93 0.2326824266375489 1101 94-95 0.2148838234729773 1101 96-97 0.15303556459311807 1101 98-99 0.12076629063903255 1101 100 0.1306866774989146 1102 1 0.303103948686676 1102 2 0.2838543172513184 1102 3 0.28633207513240677 1102 4 0.28637039683630405 1102 5 0.252317628583306 1102 6 0.18663975849240444 1102 7 0.1734910999114163 1102 8 0.1887072740741118 1102 9 0.2789474604405484 1102 10-11 0.27713245890922167 1102 12-13 0.27495616258400446 1102 14-15 0.32477935050391693 1102 16-17 0.3362044532168724 1102 18-19 0.36834774118938185 1102 20-21 0.32841481473322887 1102 22-23 0.27009336767467573 1102 24-25 0.298321707595953 1102 26-27 0.31988567180438565 1102 28-29 0.3003795125623867 1102 30-31 0.29102553347798477 1102 32-33 0.29678564725693946 1102 34-35 0.3148525352827889 1102 36-37 0.2993456256120339 1102 38-39 0.2786527763233906 1102 40-41 0.25038850795982626 1102 42-43 0.24652184311258196 1102 44-45 0.23548965004112432 1102 46-47 0.27869001709080266 1102 48-49 0.26385705168932816 1102 50-51 0.23291885410407076 1102 52-53 0.27432417209718807 1102 54-55 0.32326876046848696 1102 56-57 0.2819973445065145 1102 58-59 0.3238481421373862 1102 60-61 0.2648870190430017 1102 62-63 0.2255107978890436 1102 64-65 0.23038294647204793 1102 66-67 0.23460831469910204 1102 68-69 0.23300749612749883 1102 70-71 0.26159084003822386 1102 72-73 0.1781903379117935 1102 74-75 0.15958182265524812 1102 76-77 0.15516877590195577 1102 78-79 0.1260114724678587 1102 80-81 0.21466898660700906 1102 82-83 0.1665741759326913 1102 84-85 0.14702160200722147 1102 86-87 0.15221305035271726 1102 88-89 0.1204150132430577 1102 90-91 0.14807899624733878 1102 92-93 0.10866644686896976 1102 94-95 0.09733604316624778 1102 96-97 0.06903774594451306 1102 98-99 0.02896097949180998 1102 100 -0.009830538725609017 1103 1 0.25232220196692623 1103 2 0.21484619150438533 1103 3 0.2361696654189629 1103 4 0.20022691879251653 1103 5 0.19498714971624054 1103 6 0.11962444083365398 1103 7 0.138471916828955 1103 8 0.16113852122790462 1103 9 0.2217664215303614 1103 10-11 0.22923091669012763 1103 12-13 0.23608664038714267 1103 14-15 0.27408615693174454 1103 16-17 0.2695698381935685 1103 18-19 0.3123433074466746 1103 20-21 0.2838331752022114 1103 22-23 0.2773492395984647 1103 24-25 0.26052243796928565 1103 26-27 0.28172999396781506 1103 28-29 0.2681193078312205 1103 30-31 0.2588006366835387 1103 32-33 0.23879959361055825 1103 34-35 0.24950462799701967 1103 36-37 0.2577685608506499 1103 38-39 0.2232221884354857 1103 40-41 0.20101388684418708 1103 42-43 0.20618171998739143 1103 44-45 0.2330271224481706 1103 46-47 0.2602458166159991 1103 48-49 0.27620052691116115 1103 50-51 0.2052825620274703 1103 52-53 0.26646567772802854 1103 54-55 0.33595597458175774 1103 56-57 0.3329612099276176 1103 58-59 0.32263676884550563 1103 60-61 0.3149942410826725 1103 62-63 0.2763783484463076 1103 64-65 0.2768373619416309 1103 66-67 0.33178518510968047 1103 68-69 0.30158242536264623 1103 70-71 0.3686978077975809 1103 72-73 0.26304211208277906 1103 74-75 0.2796230184320976 1103 76-77 0.25787123169251913 1103 78-79 0.24662837281496763 1103 80-81 0.26756829644375557 1103 82-83 0.23325188549394582 1103 84-85 0.2537581451001891 1103 86-87 0.2357360702880733 1103 88-89 0.18313661158230232 1103 90-91 0.2043798678273454 1103 92-93 0.20600177468629965 1103 94-95 0.22463398901901854 1103 96-97 0.19209726974111874 1103 98-99 0.13028701724638836 1103 100 0.11478093447099269 1104 1 0.19795197220305738 1104 2 0.17183414086721172 1104 3 0.18387770821147598 1104 4 0.20288623802149885 1104 5 0.16372595180047256 1104 6 0.13857044505471094 1104 7 0.1441523674513121 1104 8 0.14991352504308253 1104 9 0.20232043523976984 1104 10-11 0.18719041561942618 1104 12-13 0.20283366440830264 1104 14-15 0.20502922442858562 1104 16-17 0.22678846195388758 1104 18-19 0.2709310803617342 1104 20-21 0.2190905872722695 1104 22-23 0.2314986627323492 1104 24-25 0.20589299729593336 1104 26-27 0.2475114622825032 1104 28-29 0.20927889223717955 1104 30-31 0.2024785532543092 1104 32-33 0.1802319690312757 1104 34-35 0.20494296953295077 1104 36-37 0.1789710651676728 1104 38-39 0.17676554938525868 1104 40-41 0.18737973102845018 1104 42-43 0.16541984061143467 1104 44-45 0.1556909746671593 1104 46-47 0.1982326478338834 1104 48-49 0.18511586656232737 1104 50-51 0.16409688491877716 1104 52-53 0.1690708877780125 1104 54-55 0.2026031200039924 1104 56-57 0.23914940872540313 1104 58-59 0.23006313235200082 1104 60-61 0.20516515580818862 1104 62-63 0.19231126981308222 1104 64-65 0.20778012279671998 1104 66-67 0.21131520598115117 1104 68-69 0.20405445695381985 1104 70-71 0.1922458817773176 1104 72-73 0.12982177964288155 1104 74-75 0.17606153907908606 1104 76-77 0.17162959140573975 1104 78-79 0.13734122663010595 1104 80-81 0.2527657091232953 1104 82-83 0.19383011486405977 1104 84-85 0.18389676485415052 1104 86-87 0.2101813409198492 1104 88-89 0.142928975520487 1104 90-91 0.155419623486015 1104 92-93 0.11343648117969707 1104 94-95 0.11020878409114232 1104 96-97 0.15891111835267324 1104 98-99 0.14987530862271825 1104 100 0.06949487341777427 1105 1 0.16514108386535042 1105 2 0.15289481331687682 1105 3 0.17677370962959316 1105 4 0.1755375760269544 1105 5 0.15699020122858087 1105 6 0.10642479681554562 1105 7 0.1087892381130402 1105 8 0.121263460424899 1105 9 0.1380673304254998 1105 10-11 0.13106937196442914 1105 12-13 0.13173258395214305 1105 14-15 0.1561024116921601 1105 16-17 0.16414664385577993 1105 18-19 0.21085101067870227 1105 20-21 0.2037551126270074 1105 22-23 0.19123282403196384 1105 24-25 0.18387261774832098 1105 26-27 0.2099888788535793 1105 28-29 0.1876971531456917 1105 30-31 0.18723216270727505 1105 32-33 0.19358211431275407 1105 34-35 0.18637833432443784 1105 36-37 0.15469960961539186 1105 38-39 0.15361261087900857 1105 40-41 0.17468018183839007 1105 42-43 0.17686904078292542 1105 44-45 0.15923727535496823 1105 46-47 0.19524614805518326 1105 48-49 0.15928920092034105 1105 50-51 0.12904538332532667 1105 52-53 0.17756143733783603 1105 54-55 0.2109946730360619 1105 56-57 0.19907519724647926 1105 58-59 0.20130521682606428 1105 60-61 0.1691678931953362 1105 62-63 0.15998885715117694 1105 64-65 0.133313424282683 1105 66-67 0.19162046278120215 1105 68-69 0.1629650732313337 1105 70-71 0.2300685462650307 1105 72-73 0.20313328011110343 1105 74-75 0.21287225223651518 1105 76-77 0.1863618400156426 1105 78-79 0.191446065415235 1105 80-81 0.19955222155582675 1105 82-83 0.2069545515361746 1105 84-85 0.18416657751183863 1105 86-87 0.1636937374242713 1105 88-89 0.1061524038768944 1105 90-91 0.16657934313625233 1105 92-93 0.2026056384048296 1105 94-95 0.15844030294414324 1105 96-97 0.1529117769521946 1105 98-99 0.11759398619632222 1105 100 0.11429879605825377 1106 1 0.11449397213370816 1106 2 0.12011534811256297 1106 3 0.15134941123711343 1106 4 0.11988923962007902 1106 5 0.10046532601519687 1106 6 0.046190262472229904 1106 7 0.08111464603192076 1106 8 0.1008095157056772 1106 9 0.13865619478825408 1106 10-11 0.10553473562256954 1106 12-13 0.09977646860617995 1106 14-15 0.11139062186073545 1106 16-17 0.0990771153004033 1106 18-19 0.16755572689061893 1106 20-21 0.14927023887208435 1106 22-23 0.15085576288434055 1106 24-25 0.11401965096597166 1106 26-27 0.16932397855411807 1106 28-29 0.11525461283343219 1106 30-31 0.14949017216991933 1106 32-33 0.1661401088782526 1106 34-35 0.0939490343778644 1106 36-37 0.09714761240086034 1106 38-39 0.0938132235441671 1106 40-41 0.09914545655417584 1106 42-43 0.09721124847519746 1106 44-45 0.08829122333447259 1106 46-47 0.09677233650969441 1106 48-49 0.11986495960157129 1106 50-51 -0.003997733883132071 1106 52-53 0.07656778966767064 1106 54-55 0.13464347563271417 1106 56-57 0.14423672581133928 1106 58-59 0.13006494909767952 1106 60-61 0.0986045851280295 1106 62-63 0.08119549289941119 1106 64-65 0.04828443292942097 1106 66-67 0.08461205920504256 1106 68-69 0.10497726782300276 1106 70-71 0.18013550998603023 1106 72-73 0.1366429160425966 1106 74-75 0.11193091585568737 1106 76-77 0.09221287231559927 1106 78-79 0.14753171450666258 1106 80-81 0.19532679551340948 1106 82-83 0.16042569735181544 1106 84-85 0.17570227348201328 1106 86-87 0.18474467132102035 1106 88-89 0.13146068424386925 1106 90-91 0.140934928852527 1106 92-93 0.13431304312336678 1106 94-95 0.14274881734720068 1106 96-97 0.11986348932149582 1106 98-99 0.078663476200596 1106 100 0.09291590241834413 1107 1 0.034941469264850866 1107 2 0.024046013724479565 1107 3 0.04198724076193372 1107 4 0.08066389525333761 1107 5 0.07064876961963762 1107 6 6.556495771690152E-4 1107 7 -0.010426740527712752 1107 8 -0.006450040038124882 1107 9 0.038268671320999204 1107 10-11 0.02386686587431086 1107 12-13 0.03278280170677306 1107 14-15 0.005060541826864551 1107 16-17 -0.005461264273428412 1107 18-19 0.06608412961053745 1107 20-21 0.033806361266890406 1107 22-23 0.03940242869352062 1107 24-25 0.013507670290231033 1107 26-27 -3.7362992149780894E-4 1107 28-29 0.05204470169240949 1107 30-31 0.01926870356070509 1107 32-33 -0.011937067148586777 1107 34-35 -0.010655252954542505 1107 36-37 -0.0019370426965537035 1107 38-39 0.03577693585447861 1107 40-41 -0.011581798499776141 1107 42-43 -0.02489469979914105 1107 44-45 0.009784582908245909 1107 46-47 0.004648746594519082 1107 48-49 -0.011943269114723876 1107 50-51 -0.059493027432274914 1107 52-53 0.03901048110130745 1107 54-55 0.05422765347000791 1107 56-57 0.09033627110857623 1107 58-59 0.0746194809516112 1107 60-61 0.09138366459900737 1107 62-63 0.11154709486908843 1107 64-65 0.09104491387142133 1107 66-67 0.13165945923144307 1107 68-69 0.12842833430553924 1107 70-71 0.13110154390696493 1107 72-73 0.08209120106492662 1107 74-75 0.09508247064325559 1107 76-77 0.09379633451812452 1107 78-79 0.11232324117390391 1107 80-81 0.14640625781896688 1107 82-83 0.052349108667502975 1107 84-85 0.11367810401547729 1107 86-87 0.15995603485019316 1107 88-89 0.10080833140848 1107 90-91 0.0989300955037109 1107 92-93 0.09663196096882487 1107 94-95 0.07232910464874465 1107 96-97 0.07641870520440364 1107 98-99 0.06688012558413803 1107 100 0.07843663202168472 1108 1 -0.025123234808795303 1108 2 -0.029333445118233215 1108 3 -0.0034745917486844746 1108 4 0.048466811737810644 1108 5 0.04588157306739049 1108 6 0.02451810506106966 1108 7 -0.019993130375659973 1108 8 0.007319528372242701 1108 9 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-0.8764253051771291 2316 52-53 -1.0917917128498473 2316 54-55 -1.0687433475617993 2316 56-57 -1.4278588095106741 2316 58-59 -1.2726176954953061 2316 60-61 -1.1480963064744785 2316 62-63 -1.1466864515130766 2316 64-65 -0.994474548350162 2316 66-67 -0.7741099504112725 2316 68-69 -0.8319091260113893 2316 70-71 -0.7940283854276657 2316 72-73 -0.8647313537134451 2316 74-75 -0.8215227302595238 2316 76-77 -0.9560942285541714 2316 78-79 -0.8008568988229321 2316 80-81 -0.7594266559736305 2316 82-83 -0.8676386033489969 2316 84-85 -1.0445767167349196 2316 86-87 -1.0643405462610716 2316 88-89 -0.8561540637884129 2316 90-91 -0.877124462136063 2316 92-93 -0.9994729428965741 2316 94-95 -1.078916003095859 2316 96-97 -0.9034632861596066 2316 98-99 -1.024494342303278 2316 100 -1.1334752351253918 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 111.0 3 180.0 4 110.0 5 94.0 6 472.0 7 4966.0 8 13271.0 9 20996.0 10 26474.0 11 29444.0 12 34607.0 13 41703.0 14 52835.0 15 68683.0 16 88777.0 17 112251.0 18 124640.0 19 138903.0 20 158254.0 21 194648.0 22 259583.0 23 385614.0 24 581816.0 25 746434.0 26 734333.0 27 597764.0 28 499489.0 29 496731.0 30 554660.0 31 663310.0 32 820652.0 33 1056106.0 34 1459203.0 35 2358702.0 36 3912088.0 37 7249337.0 38 9909750.0 39 3314426.0 40 6444.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.480385190073125 37.45704860449789 27.170556896516363 18.892009308912623 2 19.60174426505892 35.40407116828905 27.29152122182437 17.702663344827663 3 26.7533729246843 26.553137295630503 26.77516942993797 19.918320349747226 4 18.789158614979062 27.303508552515428 26.227285371092083 27.680047461413427 5 19.689792199557374 34.76929613576758 26.34960049251284 19.191311172162205 6 27.720060272836612 26.745749694635506 26.5551162477892 18.979073784738688 7 19.58049598077461 26.79398679646664 34.48122482032798 19.144292402430768 8 27.32321246975823 26.931688257134006 26.513164723102356 19.23193455000541 9 26.949823600692092 26.63034029965317 27.160511917374535 19.259324182280203 10-11 18.980617306684913 31.25544786469579 30.68315157646561 19.080783252153687 12-13 18.984716182183995 27.28548651540961 30.415437836520955 23.314359465885445 14-15 18.9955341113864 35.25256022959115 26.42872394480598 19.32318171421647 16-17 23.224428424742047 27.044745836540496 26.38418527151086 23.346640467206598 18-19 19.37352816796355 31.222767002533374 26.38060140977575 23.023103419727324 20-21 23.235170091623687 31.29262429888824 26.385019414931044 19.087186194557034 22-23 23.054605808929686 27.39540737074574 30.427108658169562 19.12287816215501 24-25 19.106030254806154 31.046970242036352 26.484990661542817 23.362008841614674 26-27 19.08781646957454 27.239398042484208 34.529099494577004 19.143685993364244 28-29 19.14373029627409 27.266754816532917 34.51224836253869 19.0772665246543 30-31 19.157024667330084 27.215284274136163 34.49782364711828 19.12986741141547 32-33 19.18306627635517 27.280635743222742 34.40147227223439 19.13482570818769 34-35 19.198917258696625 27.40501266004889 34.28667584412231 19.109394237132182 36-37 19.15011163868932 27.399478705765564 34.30474043986007 19.145669215685043 38-39 19.199095818584514 27.372539206217905 34.24902398482655 19.17934099037103 40-41 19.272855524309886 27.4329854155872 34.17527118021078 19.118887879892135 42-43 19.29846188810523 27.489173904695825 34.14617558134502 19.066188625853922 44-45 19.373929934357164 27.55316870842596 34.00223972888482 19.070661628332058 46-47 19.303392539373327 27.641324577247367 33.97324232119836 19.08204056218094 48-49 19.40982811199028 27.666116691816367 33.79544865614305 19.1286065400503 50-51 19.469280906657353 27.751180157029086 33.656431160805475 19.12310777550809 52-53 19.554993719654057 27.946763931560426 33.415744929889975 19.082497418895542 54-55 19.95721087816848 28.200642286201493 32.527920731294394 19.31422610433563 56-57 20.477218417438927 28.865402454561846 31.20200376175139 19.45537536624784 58-59 20.67937287434155 29.415388191475504 30.486100666237803 19.419138267945144 60-61 20.794466130646974 29.774748219960596 30.044821097932072 19.38596455146036 62-63 20.888281897947635 29.954519403021994 29.799814080647025 19.357384618383342 64-65 21.010036581913713 30.005654972433167 29.74415948144431 19.24014896420881 66-67 21.14075941023435 29.814434889984447 29.89591336680742 19.148892332973784 68-69 21.25658873758826 29.54406868011289 30.15086309938051 19.048479482918342 70-71 21.39719618150359 29.391166016242583 30.29314975296231 18.918488049291515 72-73 21.56683192349608 29.308530419816442 30.25729626354885 18.86734139313863 74-75 21.775869241133993 29.365805261465606 30.059791214034075 18.798534283366326 76-77 22.052781907977355 29.52691389472128 29.683345373961316 18.736958823340046 78-79 22.35062751443928 29.668157678178193 29.28884647402128 18.69236833336125 80-81 22.633082078499307 29.807077137128363 28.91831373697609 18.641527047396245 82-83 22.804615089679668 29.948734768665027 28.60980056475822 18.63684957689709 84-85 23.008807882845698 29.963113780048765 28.386649230046324 18.64142910705921 86-87 23.225258762986936 29.936981591751525 28.21135120101626 18.626408444245275 88-89 23.4225025869822 29.944049501090227 28.064333507335338 18.569114404592238 90-91 23.661102353866827 29.90175260696688 27.86820963630838 18.568935402857914 92-93 23.8325505739169 29.87770808870825 27.76318458902618 18.526556748348668 94-95 24.012806613761793 29.84754179734817 27.663704427746932 18.475947161143104 96-97 24.148946721123227 29.82160074683374 27.536157992600923 18.493294539442108 98-99 24.294534869378847 29.831271073037435 27.413287552971006 18.460906504612712 100 24.39433324870365 29.87084889292417 27.29676094391596 18.438056914456226 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 199840.0 1 120018.5 2 32193.0 3 22800.5 4 21202.0 5 20989.0 6 21344.5 7 21537.0 8 21334.0 9 21153.5 10 22399.0 11 32550.5 12 281070.5 13 326634.0 14 123142.0 15 120355.0 16 134697.0 17 149047.0 18 162089.0 19 175335.5 20 193645.5 21 223460.0 22 264758.0 23 307463.0 24 345042.5 25 388492.5 26 440334.0 27 500758.5 28 580759.5 29 659188.0 30 717732.5 31 781849.0 32 854082.5 33 906642.0 34 945636.0 35 1001196.5 36 1061509.5 37 1103809.5 38 1144418.0 39 1165503.0 40 1163979.0 41 1174024.0 42 1201197.5 43 1200650.0 44 1177152.5 45 1168826.0 46 1159785.5 47 1151910.0 48 1129773.0 49 1093882.5 50 1064592.0 51 1033136.5 52 989258.0 53 927031.5 54 850875.0 55 773650.5 56 699186.5 57 626105.5 58 550446.0 59 466500.5 60 380699.5 61 306090.0 62 239437.5 63 177687.5 64 128706.5 65 91901.0 66 65251.0 67 48616.0 68 36672.5 69 27088.0 70 20401.0 71 16260.0 72 13550.5 73 10931.5 74 9109.0 75 8357.0 76 6477.0 77 3648.5 78 2070.0 79 1470.0 80 1064.0 81 800.0 82 645.0 83 528.0 84 440.5 85 355.5 86 270.5 87 233.5 88 201.0 89 161.5 90 142.0 91 116.5 92 97.0 93 83.0 94 70.0 95 56.0 96 41.0 97 28.5 98 20.0 99 14.5 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004493725819159237 2 1.2800309909120252E-4 3 0.09009511746885256 4 0.025339166679671236 5 0.017795154243870577 6 0.05646570752038089 7 0.03785078874828792 8 0.02595739441357981 9 0.0054850689695677 10-11 0.007593034899282395 12-13 0.014807507441678044 14-15 0.026758094650448185 16-17 0.04935608858043229 18-19 0.06249547052863456 20-21 0.045202796535451784 22-23 0.026758094650448185 24-25 0.019152804135295354 26-27 0.017520083754334163 28-29 0.012601496584999872 30-31 0.010665109277471257 32-33 0.007930745203267696 34-35 0.00589767470387232 36-37 0.009974709583436792 38-39 0.014480691018466463 40-41 0.016142007836458663 42-43 0.009701000828997091 44-45 0.003713451608741588 46-47 0.002720746723236411 48-49 0.001981324565720209 50-51 0.001077132461501502 52-53 0.0014611417587751096 54-55 3.200077477280063E-4 56-57 0.0025083160481488833 58-59 0.01073864297269386 60-61 0.022169047374518903 62-63 0.02726057490113599 64-65 0.008905747532515579 66-67 0.002185584830227447 68-69 0.0015401223943845748 70-71 0.002154264923003004 72-73 7.244430714523376E-4 74-75 7.053787800983287E-4 76-77 0.001380799388068929 78-79 0.0032028009474734925 80-81 0.007480010886255057 82-83 0.008746424526199933 84-85 0.007612099190636405 86-87 0.004587685540832566 88-89 0.002319034869705509 90-91 0.0026090844453057873 92-93 0.002614531385692647 94-95 0.003288590258566533 96-97 0.0034193168278511647 98-99 0.007752357905598041 100 0.013546540742120028 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.6717861E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.59295817118312 #Duplication Level Percentage of deduplicated Percentage of total 1 76.43865670702986 30.26432537662859 2 11.715906726138957 9.277348098910053 3 3.9865993884727646 4.735237884991991 4 2.0606069715769113 3.2634210253157194 5 1.1888344527130092 2.353473637936377 6 0.7989842010943717 1.8980488832019373 7 0.5904383678846867 1.6364041121624022 8 0.4255359248105055 1.3478580857086455 9 0.32657764466340256 1.163715752232144 >10 2.0857586722867856 15.24224362181495 >50 0.19745862798399277 5.411811956987634 >100 0.18186289371370412 14.279454268251099 >500 0.0020430739396522834 0.4827660854076295 >1k 5.308711338963386E-4 0.49108934720201414 >5k 1.369843729577338E-4 0.3455087785984083 >10k+ 6.849218450250455E-5 7.807293084650321 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2451664 6.677033828304976 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 192837 0.5251858216904302 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 77492 0.21104715222926518 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 54224 0.1476774477685397 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.09754108497768973 5.446940386859681E-6 0.0 2.7234701934298405E-6 5.446940386859681E-6 2 0.09754380844788317 8.170410580289522E-6 0.0 5.446940386859681E-6 1.3617350967149203E-5 3 0.09754380844788317 8.170410580289522E-6 0.0 8.170410580289522E-6 1.3617350967149203E-5 4 0.09754925538827003 1.0893880773719362E-5 0.0 8.170410580289522E-6 2.9958172127728246E-5 5 0.09754925538827003 1.9064291354008884E-5 0.0 8.170410580289522E-6 3.540511251458793E-5 6 0.09754925538827003 2.7234701934298406E-5 0.0 8.170410580289522E-6 3.812858270801777E-5 7 0.09755197885846346 2.9958172127728246E-5 0.0 8.170410580289522E-6 4.902246348173713E-5 8 0.09755470232865689 2.9958172127728246E-5 0.0 8.170410580289522E-6 5.174593367516697E-5 9 0.09756014926904374 3.268164232115809E-5 0.0 8.170410580289522E-6 5.174593367516697E-5 10-11 0.09756151100414046 3.540511251458793E-5 0.0 1.0893880773719362E-5 5.99163442554565E-5 12-13 0.09756559620943062 3.812858270801777E-5 0.0 1.7702556257293964E-5 7.081022502917586E-5 14-15 0.09756831967962404 4.085205290144761E-5 0.0 2.4511231740868563E-5 8.442757599632505E-5 16-17 0.09757240488491419 5.446940386859681E-5 0.0 3.268164232115809E-5 8.987451638318473E-5 18-19 0.09758057529549446 5.719287406202665E-5 0.0 3.676684761130285E-5 1.075770726404787E-4 20-21 0.09759691611665505 6.944848993246094E-5 0.0 3.812858270801777E-5 1.1710921831748314E-4 22-23 0.09766772634168422 7.35336952226057E-5 0.0 3.812858270801777E-5 1.2936483418791745E-4 24-25 0.09797003153315494 7.489543031932062E-5 0.0 4.221378799816253E-5 1.552378010255009E-4 26-27 0.09933993704045015 8.442757599632505E-5 0.0 4.357552309487745E-5 1.7157862218607995E-4 28-29 0.10380370468748165 8.578931109303997E-5 0.0 4.766072838502221E-5 1.8928117844337392E-4 30-31 0.11527359940711143 9.668319186675935E-5 0.0 5.446940386859681E-5 2.151541452809574E-4 32-33 0.13872948644802593 1.0349186735033395E-4 0.0 6.263981444888634E-5 2.3013323134482154E-4 34-35 0.17996554864674713 1.0893880773719362E-4 0.0 6.263981444888634E-5 2.410271121185409E-4 36-37 0.24442055597955448 1.1847095341419806E-4 0.0 6.944848993246094E-5 2.5192099289226026E-4 38-39 0.33735625286015436 1.2527962889777268E-4 0.0 7.217196012589078E-5 2.669000789561244E-4 40-41 0.4612264859328271 1.3208830438134726E-4 0.0 7.625716541603554E-5 2.7779395972984375E-4 42-43 0.6191305642776959 1.402587149616368E-4 0.0 7.625716541603554E-5 2.941347808904228E-4 44-45 0.812012170316784 1.457056553484965E-4 0.0 8.170410580289522E-5 3.118373371477168E-4 46-47 1.0452256464503746 1.5115259573535617E-4 0.0 8.442757599632505E-5 3.2817815830829577E-4 48-49 1.319496797485017 1.5796127121893075E-4 0.0 8.578931109303997E-5 3.4588071456558977E-4 50-51 1.6315179688707901 1.5932300631564566E-4 0.0 8.851278128646981E-5 3.5813633043602403E-4 52-53 1.9809963875619006 1.6613168179922027E-4 0.0 9.395972167332951E-5 3.690302112097434E-4 54-55 2.366263928064873 1.6613168179922027E-4 0.0 9.668319186675935E-5 3.7447715159660307E-4 56-57 2.791367667087143 1.674934168959352E-4 0.0 9.804492696347427E-5 3.7856235688674784E-4 58-59 3.253759798262758 1.743020923795098E-4 0.0 1.007683971569041E-4 3.92179707853897E-4 60-61 3.749394606619378 1.8519597315322915E-4 0.0 1.0621533754376379E-4 4.057970588210463E-4 62-63 4.274547746667487 1.947281188302336E-4 0.0 1.1983268851091298E-4 4.126057343046208E-4 64-65 4.827146657589886 2.0289852941052315E-4 0.0 1.2255615870434284E-4 4.2758482036848495E-4 66-67 5.411550253431157 2.069837347006679E-4 0.0 1.2800309909120252E-4 4.43925641529064E-4 68-69 6.022297976453476 2.1106893999081264E-4 0.0 1.334500394780622E-4 4.684368732699326E-4 70-71 6.660668495912656 2.137924101842425E-4 0.0 1.3889697986492187E-4 4.7660728385022207E-4 72-73 7.322324685525663 2.1651588037767232E-4 2.7234701934298405E-6 1.4298218515506662E-4 4.902246348173712E-4 74-75 8.005934768367908 2.2468629095796186E-4 2.7234701934298405E-6 1.4434392025178155E-4 5.065654559779503E-4 76-77 8.709565080602054 2.3149496644153645E-4 2.7234701934298405E-6 1.4434392025178155E-4 5.13374131461525E-4 78-79 9.435203755469306 2.342184366349663E-4 2.7234701934298405E-6 1.4979086063864123E-4 5.201828069450996E-4 80-81 10.180044256935338 2.423888472152558E-4 2.7234701934298405E-6 1.4979086063864123E-4 5.324384228155338E-4 82-83 10.94686997153783 2.56006198182405E-4 2.7234701934298405E-6 1.4979086063864123E-4 5.433323035892532E-4 84-85 11.73583205187252 2.6690007895612434E-4 2.7234701934298405E-6 1.4979086063864123E-4 5.596731247498322E-4 86-87 12.539890327489392 2.914113106969929E-4 2.7234701934298405E-6 1.5387606592878598E-4 5.705670055235516E-4 88 13.153873533101507 3.07752131857572E-4 2.7234701934298405E-6 1.552378010255009E-4 5.746522108136964E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 316345 0.0 86.59177 8 GTGGTAT 317885 0.0 86.32554 6 AGTGGTA 319220 0.0 86.229515 5 TGGTATC 318070 0.0 85.9173 7 AAGCAGT 330045 0.0 85.19346 1 GCAGTGG 329660 0.0 84.80197 3 GTATCAA 321275 0.0 84.40604 9 AGCAGTG 340965 0.0 82.632065 2 CAGTGGT 336680 0.0 82.265755 4 CAACGCA 312630 0.0 45.4891 12-13 ATCAACG 313870 0.0 45.324303 10-11 ACGCAGA 319215 0.0 44.896374 14-15 AGAGTAC 319830 0.0 44.43886 18-19 TCAACGC 312485 0.0 43.36606 12-13 AACGCAG 314565 0.0 42.838142 14-15 AGTACTT 329375 0.0 42.81308 20-21 TATCAAC 317520 0.0 42.61209 10-11 CGCAGAG 320635 0.0 41.942677 16-17 GCAGAGT 341120 0.0 41.774727 16-17 GAGTACT 316230 0.0 41.59144 20-21 >>END_MODULE