FastQCFastQC Report
Thu 26 May 2016
SRR1660688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36717861
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23045396.276343276096612No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1879230.5118026891599159No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT876390.2386822042819978No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT568600.15485651519842072No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2977300.085.54218
GTGGTAT3010250.084.8720256
AGTGGTA3022600.084.840835
TGGTATC3007700.084.5580147
AAGCAGT3084400.084.111661
GCAGTGG3111300.083.3940053
GTATCAA3034600.083.200299
AGCAGTG3217250.081.169382
CAGTGGT3196500.080.749674
CAACGCA2935800.045.0080112-13
ATCAACG2938200.044.86416610-11
ACGCAGA3006300.044.32313514-15
AGAGTAC3031900.043.8677818-19
TCAACGC2936650.042.81989312-13
AGTACTT3125350.042.33515520-21
AACGCAG2958400.042.2828114-15
TATCAAC2996300.042.05382510-11
CGCAGAG3016750.041.4623316-17
GAGTACT2999650.041.17828420-21
GCAGAGT3252550.041.05020516-17
Produced by FastQC (version 0.11.5)