FastQCFastQC Report
Thu 26 May 2016
SRR1660687_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660687_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41844042
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30417167.2691734703831905No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1934500.4623119343967775No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT671520.16048162842394623No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT581050.13886086817329932No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA3980100.086.579425
GGTATCA3980450.086.307838
AAGCAGT4109800.086.0820541
TGGTATC3982750.085.775997
GTGGTAT4008550.085.718036
GCAGTGG4129650.085.216513
GTATCAA4033700.084.504069
CAGTGGT4152150.083.561724
AGCAGTG4276150.082.973662
CAACGCA3979750.045.14049512-13
ATCAACG3984000.045.07877310-11
ACGCAGA4042800.044.7125214-15
AGAGTAC4012800.044.2864618-19
AGTACTT4070300.043.52460520-21
TCAACGC3988800.042.89261612-13
TATCAAC4006550.042.44066210-11
AACGCAG4009700.042.4212214-15
GAGTACT3947950.041.72746720-21
CGCAGAG4056450.041.69505716-17
GCAGAGT4305100.041.43694716-17
Produced by FastQC (version 0.11.5)