##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660687_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 41844042 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.291851465974535 31.0 31.0 34.0 28.0 34.0 2 31.42800578395366 31.0 31.0 34.0 28.0 34.0 3 31.480581823333416 33.0 31.0 34.0 28.0 34.0 4 35.02413411687141 37.0 35.0 37.0 32.0 37.0 5 34.98522506501642 37.0 35.0 37.0 32.0 37.0 6 35.02810337968784 37.0 35.0 37.0 32.0 37.0 7 34.91170262662484 37.0 35.0 37.0 32.0 37.0 8 34.888069871452664 37.0 35.0 37.0 32.0 37.0 9 36.48722487182285 39.0 37.0 39.0 32.0 39.0 10-11 36.376703151669716 39.0 36.0 39.0 32.0 39.0 12-13 36.46592569857377 39.0 37.0 39.0 32.0 39.0 14-15 37.73919596486401 40.0 37.0 41.0 32.0 41.0 16-17 37.629914672201124 40.0 37.0 41.0 32.0 41.0 18-19 37.56169347836904 40.0 37.0 41.0 32.0 41.0 20-21 37.4357167503082 40.0 37.0 41.0 31.0 41.0 22-23 37.21659333245101 39.5 36.5 41.0 31.0 41.0 24-25 37.27691527744858 39.5 37.0 41.0 31.0 41.0 26-27 37.31573864207478 40.0 37.0 41.0 31.0 41.0 28-29 37.21399545961645 40.0 36.5 41.0 30.5 41.0 30-31 37.0293325032988 40.0 36.0 41.0 30.0 41.0 32-33 36.91202875429673 40.0 36.0 41.0 30.0 41.0 34-35 36.75732042090962 39.0 36.0 41.0 30.0 41.0 36-37 36.497012430108924 39.0 35.5 40.5 29.5 41.0 38-39 35.974017925897314 38.0 35.0 40.0 29.0 41.0 40-41 36.08128415271163 38.0 35.0 40.0 29.0 41.0 42-43 35.93808387344607 38.0 35.0 40.0 28.0 41.0 44-45 35.77508556654254 38.0 35.0 40.0 27.0 41.0 46-47 35.72640334315695 38.0 35.0 40.0 27.0 41.0 48-49 35.78625830410934 38.0 35.0 40.0 27.0 41.0 50-51 34.76966544006432 37.5 34.0 39.5 25.5 40.5 52-53 34.692688089262504 37.5 33.0 39.5 24.5 40.5 54-55 34.72925336658442 38.0 33.0 40.0 22.5 41.0 56-57 34.297838686329584 38.0 33.0 40.0 20.0 41.0 58-59 34.067315186711646 38.0 33.0 40.0 18.0 41.0 60-61 33.88601659227854 37.5 33.0 40.0 18.0 41.0 62-63 33.63761877975364 37.0 33.0 40.0 18.0 41.0 64-65 33.37453732361706 36.5 32.0 40.0 18.0 41.0 66-67 33.0940325148321 36.0 32.0 39.5 17.0 41.0 68-69 32.80426101044445 35.5 31.5 39.0 17.0 41.0 70-71 32.557838747987105 35.0 31.0 39.0 17.5 41.0 72-73 32.25275063054377 35.0 31.0 38.5 17.0 40.0 74-75 31.88285656055885 35.0 31.0 37.0 16.0 40.0 76-77 31.492926507912408 35.0 30.5 37.0 15.0 39.0 78-79 31.140035121368058 35.0 30.0 36.0 12.5 39.0 80-81 30.79478209824949 35.0 30.0 36.0 11.5 38.5 82-83 30.49311669030444 34.5 29.5 35.0 10.0 37.0 84-85 30.203253488274388 34.0 29.0 35.0 10.0 37.0 86-87 29.941014637161487 34.0 29.0 35.0 10.0 36.0 88-89 29.722882423739083 34.0 29.0 35.0 10.0 36.0 90-91 29.515201865536795 34.0 29.0 35.0 10.0 36.0 92-93 29.35986892709839 34.0 28.5 35.0 10.0 35.0 94-95 29.226684267260794 34.0 28.0 35.0 10.0 35.0 96-97 29.05014486889197 34.0 27.0 35.0 10.0 35.0 98-99 28.901691786849845 34.0 27.0 35.0 10.0 35.0 100 28.786645945915073 34.0 27.0 35.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5349799511484754 1101 2 0.4646344422265365 1101 3 0.5318678217773254 1101 4 0.4348311808714129 1101 5 0.5123677581579074 1101 6 0.5281048595197149 1101 7 0.536964977423807 1101 8 0.5935643488186386 1101 9 0.6501459090970627 1101 10-11 0.6345064182114228 1101 12-13 0.5759723150658829 1101 14-15 0.6127174374236475 1101 16-17 0.6080000275755424 1101 18-19 0.6834310381907045 1101 20-21 0.6826160160420969 1101 22-23 0.6307554973534266 1101 24-25 0.6419865180703539 1101 26-27 0.6149199215516035 1101 28-29 0.6312996573628524 1101 30-31 0.5254162867578458 1101 32-33 0.5504317049260621 1101 34-35 0.48951273877870705 1101 36-37 0.5623328550398057 1101 38-39 0.5454105872099575 1101 40-41 0.5508037103581174 1101 42-43 0.5294173598066436 1101 44-45 0.5036717974230456 1101 46-47 0.509076884526209 1101 48-49 0.535474309678861 1101 50-51 0.5001104928143079 1101 52-53 0.5487990278757806 1101 54-55 0.59384232070677 1101 56-57 0.5712374347563625 1101 58-59 0.5119307471229959 1101 60-61 0.4363001181172663 1101 62-63 0.44890739403656 1101 64-65 0.33904620018997633 1101 66-67 0.38692195973542454 1101 68-69 0.292354721586328 1101 70-71 0.27666722781759745 1101 72-73 0.26873293434986323 1101 74-75 0.22550747606995358 1101 76-77 0.2585597345498378 1101 78-79 0.1938120545381139 1101 80-81 0.1496006896471016 1101 82-83 0.0864310055895352 1101 84-85 0.07283891562062905 1101 86-87 0.08945659654315463 1101 88-89 0.05128985029023525 1101 90-91 0.07417457281457018 1101 92-93 0.025684478322279602 1101 94-95 -0.05244067068637648 1101 96-97 -0.07541176465743149 1101 98-99 -0.04081745782587021 1101 100 -0.0418126604012663 1102 1 0.48634931317890207 1102 2 0.4579692871814167 1102 3 0.495115492763194 1102 4 0.408908069106225 1102 5 0.3497461464375462 1102 6 0.41350780578031276 1102 7 0.5171118646362984 1102 8 0.5888927554874712 1102 9 0.5824326720894746 1102 10-11 0.5629470626005997 1102 12-13 0.49725620100648626 1102 14-15 0.5742977191709784 1102 16-17 0.6334745488769187 1102 18-19 0.5475984117081367 1102 20-21 0.5500623895607859 1102 22-23 0.5890278253182899 1102 24-25 0.5211837428194315 1102 26-27 0.5052677568095234 1102 28-29 0.49848732439957644 1102 30-31 0.4701893072597869 1102 32-33 0.43133072567199093 1102 34-35 0.4155987481283674 1102 36-37 0.48270879783439113 1102 38-39 0.45392462489784435 1102 40-41 0.46924629877405266 1102 42-43 0.47817225737961166 1102 44-45 0.43374108526783317 1102 46-47 0.4285732899632322 1102 48-49 0.49554953381237965 1102 50-51 0.4469548271171959 1102 52-53 0.4523621486460172 1102 54-55 0.5519376919287424 1102 56-57 0.5597680901000004 1102 58-59 0.5042696736357328 1102 60-61 0.44381733901422393 1102 62-63 0.47043479538124444 1102 64-65 0.2998337430389668 1102 66-67 0.2786575462233216 1102 68-69 0.38334575660449843 1102 70-71 0.4126871240870571 1102 72-73 0.32374902647158166 1102 74-75 0.2990913235432693 1102 76-77 0.25914408348144136 1102 78-79 0.2147472823333132 1102 80-81 0.254592292781048 1102 82-83 0.21637142530167708 1102 84-85 0.18594627351038895 1102 86-87 0.13635479583895815 1102 88-89 0.18122543688593495 1102 90-91 0.15932578377974593 1102 92-93 0.0855362920974514 1102 94-95 0.06081323484002965 1102 96-97 0.06261368628221575 1102 98-99 0.014104532261033143 1102 100 0.004281058577628727 1103 1 0.47437740122217065 1103 2 0.4622801506782821 1103 3 0.37605099858725666 1103 4 0.35473250057934536 1103 5 0.4476665734564875 1103 6 0.4210181722777975 1103 7 0.40745007150628254 1103 8 0.48056570329210757 1103 9 0.5207680360630818 1103 10-11 0.5154935883535998 1103 12-13 0.5344709228941369 1103 14-15 0.539834885162982 1103 16-17 0.5908627060594469 1103 18-19 0.5997453916788373 1103 20-21 0.612205712000538 1103 22-23 0.6075972143428316 1103 24-25 0.5447468781996037 1103 26-27 0.5090230545658443 1103 28-29 0.4354868320935381 1103 30-31 0.44052364395029997 1103 32-33 0.49711794618607996 1103 34-35 0.5222399317667481 1103 36-37 0.5000240822185802 1103 38-39 0.5070321756652021 1103 40-41 0.47702774002893733 1103 42-43 0.4774955184852132 1103 44-45 0.4344819192630709 1103 46-47 0.45784146774370527 1103 48-49 0.4293089024455128 1103 50-51 0.37489327904164327 1103 52-53 0.3829732247749149 1103 54-55 0.5139479883558309 1103 56-57 0.5854080900185252 1103 58-59 0.5182203966159662 1103 60-61 0.4301104432867291 1103 62-63 0.43649894290242486 1103 64-65 0.3561338271516945 1103 66-67 0.33026118038413443 1103 68-69 0.2523812515158994 1103 70-71 0.33298039053053685 1103 72-73 0.30311305092036633 1103 74-75 0.2966936090697523 1103 76-77 0.2700946134648916 1103 78-79 0.23574703255961538 1103 80-81 0.1788334375304217 1103 82-83 0.1837110456367057 1103 84-85 0.12017215543007964 1103 86-87 0.09809218420996402 1103 88-89 0.11261131602118368 1103 90-91 0.1100007847493778 1103 92-93 0.08566648485709294 1103 94-95 0.1267870132172746 1103 96-97 0.14305420941028402 1103 98-99 0.12726744105396293 1103 100 0.10036171267504912 1104 1 0.4480729621388839 1104 2 0.49377760035622487 1104 3 0.41759337381951767 1104 4 0.3804872382604856 1104 5 0.44979032587374235 1104 6 0.42424004507535074 1104 7 0.4927317336499186 1104 8 0.56402485430133 1104 9 0.60163031380673 1104 10-11 0.5880578931952485 1104 12-13 0.5365896344546854 1104 14-15 0.5448310064021271 1104 16-17 0.60287747857938 1104 18-19 0.5575660104442051 1104 20-21 0.507660319690963 1104 22-23 0.5845955133531433 1104 24-25 0.5424269210775776 1104 26-27 0.4414506938371261 1104 28-29 0.4667895172714509 1104 30-31 0.4620658660091266 1104 32-33 0.4391908061342136 1104 34-35 0.4928152517067659 1104 36-37 0.5285959576125947 1104 38-39 0.48806368214566476 1104 40-41 0.4955932354532848 1104 42-43 0.45475301343173413 1104 44-45 0.43683629725028794 1104 46-47 0.43932682228015096 1104 48-49 0.4718301124491333 1104 50-51 0.387251025151798 1104 52-53 0.4202852414280258 1104 54-55 0.48228778181032794 1104 56-57 0.46692880375320556 1104 58-59 0.47093462781889883 1104 60-61 0.40735635830334616 1104 62-63 0.45044095177708954 1104 64-65 0.40245910381999295 1104 66-67 0.2918639337296085 1104 68-69 0.3909612474562678 1104 70-71 0.3674702560785619 1104 72-73 0.2819564029736341 1104 74-75 0.2610296535325922 1104 76-77 0.2627622071783726 1104 78-79 0.24532674732179416 1104 80-81 0.21821402453551997 1104 82-83 0.17750482200349538 1104 84-85 0.21136578095152458 1104 86-87 0.15761868017021285 1104 88-89 0.1676578714422341 1104 90-91 0.20003705036578978 1104 92-93 0.19978057305944574 1104 94-95 0.15846513810687313 1104 96-97 0.13553125834794955 1104 98-99 0.07531013656489094 1104 100 0.09441326579206333 1105 1 0.4095298255861657 1105 2 0.3798981852692833 1105 3 0.3603608968287446 1105 4 0.32266957577542854 1105 5 0.40666219702177386 1105 6 0.37078638847992806 1105 7 0.40007841251726717 1105 8 0.4641377519649623 1105 9 0.48539397624728053 1105 10-11 0.4565746543535738 1105 12-13 0.4692913524948068 1105 14-15 0.5051997023963182 1105 16-17 0.5236442388833424 1105 18-19 0.5105544116616016 1105 20-21 0.5514642844285831 1105 22-23 0.546828973481162 1105 24-25 0.4721144778501909 1105 26-27 0.43286155501967016 1105 28-29 0.42752458915730784 1105 30-31 0.4793189372675357 1105 32-33 0.41180718707398256 1105 34-35 0.4502914777771636 1105 36-37 0.4939338297523932 1105 38-39 0.4242540374788746 1105 40-41 0.3852784323136902 1105 42-43 0.40528102607797933 1105 44-45 0.4279085731194243 1105 46-47 0.35930497938126393 1105 48-49 0.4360532845772127 1105 50-51 0.29651259940595764 1105 52-53 0.39389702223542855 1105 54-55 0.4861964113313064 1105 56-57 0.4752668414811012 1105 58-59 0.4018921938880524 1105 60-61 0.32293912719166684 1105 62-63 0.3551931052639432 1105 64-65 0.32595559800962803 1105 66-67 0.3505180576274043 1105 68-69 0.4009203560018122 1105 70-71 0.36889707861622867 1105 72-73 0.32057312618884026 1105 74-75 0.27826890742638355 1105 76-77 0.3070537595748384 1105 78-79 0.2688001719552098 1105 80-81 0.24299283832907292 1105 82-83 0.2515163227450401 1105 84-85 0.16523565293583076 1105 86-87 0.27652642719551324 1105 88-89 0.22268198455059363 1105 90-91 0.2506360546147661 1105 92-93 0.24955209766386233 1105 94-95 0.20161877513770676 1105 96-97 0.24958386392075127 1105 98-99 0.2608826281012604 1105 100 0.22079788131716427 1106 1 0.35694816415576014 1106 2 0.3579222537672031 1106 3 0.3318323484209529 1106 4 0.2636536017673947 1106 5 0.33621870896616457 1106 6 0.3710479316994224 1106 7 0.40466957173615015 1106 8 0.46428096633861315 1106 9 0.5101229703977808 1106 10-11 0.39751268196047107 1106 12-13 0.34360148704877247 1106 14-15 0.4249076288022451 1106 16-17 0.5200742168433479 1106 18-19 0.43898178568824164 1106 20-21 0.5136512428772093 1106 22-23 0.5202789992223629 1106 24-25 0.41675081668349634 1106 26-27 0.38201738765535254 1106 28-29 0.4146304042358224 1106 30-31 0.3906287979335872 1106 32-33 0.34672357280388155 1106 34-35 0.40328762238370786 1106 36-37 0.41416659255251886 1106 38-39 0.4236456169714131 1106 40-41 0.41211677835237737 1106 42-43 0.37664174013620766 1106 44-45 0.3648650112849694 1106 46-47 0.3790339929008013 1106 48-49 0.43080642234805566 1106 50-51 0.3925805713933386 1106 52-53 0.3018748425122979 1106 54-55 0.34784440012217743 1106 56-57 0.3730531125791856 1106 58-59 0.3719826488587543 1106 60-61 0.36418550732808086 1106 62-63 0.37642806404640083 1106 64-65 0.3731943672690079 1106 66-67 0.32040245307261017 1106 68-69 0.37585382889515273 1106 70-71 0.42464129834557696 1106 72-73 0.33720675701088254 1106 74-75 0.3175923993793397 1106 76-77 0.3355280164647816 1106 78-79 0.2845269765960694 1106 80-81 0.21361689172895382 1106 82-83 0.23005343459393757 1106 84-85 0.2214538347045334 1106 86-87 0.25763092255648345 1106 88-89 0.3105247777924589 1106 90-91 0.3396506436144584 1106 92-93 0.3375410964462624 1106 94-95 0.2637612510118643 1106 96-97 0.3098934777664972 1106 98-99 0.30245158229467606 1106 100 0.28488159691917403 1107 1 0.35229230185767335 1107 2 0.18451611797099332 1107 3 0.3546875704079895 1107 4 0.280089710386342 1107 5 0.3399382506462132 1107 6 0.34795572628174654 1107 7 0.3624610356347233 1107 8 0.41164399611157876 1107 9 0.4585276385716952 1107 10-11 0.40266875982438677 1107 12-13 0.4535889907266153 1107 14-15 0.388951987738821 1107 16-17 0.4624385171594838 1107 18-19 0.42361594023625315 1107 20-21 0.39397268523325124 1107 22-23 0.4489843447867017 1107 24-25 0.44117926815432895 1107 26-27 0.36093896485561316 1107 28-29 0.4052558935336634 1107 30-31 0.3822390303522525 1107 32-33 0.3430715216650029 1107 34-35 0.34872909438274036 1107 36-37 0.38157343776695285 1107 38-39 0.3817080575980185 1107 40-41 0.3897277546637312 1107 42-43 0.39389553333301563 1107 44-45 0.4519637770605911 1107 46-47 0.3708351401049583 1107 48-49 0.42972143350417014 1107 50-51 0.2900866856855444 1107 52-53 0.27610394229964896 1107 54-55 0.3963799292065886 1107 56-57 0.4006355766070797 1107 58-59 0.36707877893148577 1107 60-61 0.34491116918739095 1107 62-63 0.371693080440501 1107 64-65 0.36442208432131196 1107 66-67 0.35872321139141405 1107 68-69 0.3872131850817908 1107 70-71 0.43178731721923924 1107 72-73 0.35226643645876266 1107 74-75 0.35396035106990453 1107 76-77 0.3382992439294874 1107 78-79 0.29855694084409734 1107 80-81 0.2559189061811651 1107 82-83 0.23924650523548863 1107 84-85 0.24939646544543592 1107 86-87 0.27158411859739573 1107 88-89 0.33263054528152836 1107 90-91 0.311669696643051 1107 92-93 0.27820398107101596 1107 94-95 0.29670242853267936 1107 96-97 0.2819590755608665 1107 98-99 0.34492225926381437 1107 100 0.28409828032823015 1108 1 0.2953433416280298 1108 2 0.17582179724205105 1108 3 0.29140191436461294 1108 4 0.263912582615049 1108 5 0.2827467181255088 1108 6 0.33431935266986557 1108 7 0.3212578177869432 1108 8 0.47531160557994667 1108 9 0.4402913381733242 1108 10-11 0.4504052978857018 1108 12-13 0.3565005092226414 1108 14-15 0.37701610854204404 1108 16-17 0.4505932858658852 1108 18-19 0.37341725949271165 1108 20-21 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60170.0 13 74797.0 14 97844.0 15 130815.0 16 171969.0 17 214355.0 18 242889.0 19 273978.0 20 311386.0 21 381006.0 22 494627.0 23 680706.0 24 883163.0 25 994866.0 26 954063.0 27 834477.0 28 778197.0 29 820215.0 30 942077.0 31 1121045.0 32 1372515.0 33 1736695.0 34 2285774.0 35 3307296.0 36 4954554.0 37 7549408.0 38 7954509.0 39 2050516.0 40 3135.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.13140001837313 37.570590063385396 26.455349033599028 18.84266088464244 2 20.128777714632314 36.088935847022064 25.61741368176884 18.16487275657678 3 28.491781299888153 25.526914510223786 25.936391706258544 20.044912483629513 4 19.357630509913587 26.48723750462345 24.95449916435523 29.20063282110773 5 20.159023053761423 34.91434855134865 25.394261413045466 19.532366981844458 6 29.391340930939936 26.061606443767626 25.35231661950216 19.194736005790272 7 20.188570300709372 25.718017122779997 34.44851371007037 19.644898866440254 8 29.170074560706688 26.22582617771178 25.129225143356194 19.47487411822534 9 28.483401335865594 25.86677000279371 25.961791921093642 19.688036740247057 10-11 19.46303379509054 31.023208919663464 30.03969383886786 19.474063446378135 12-13 19.419568961861422 26.583528708705558 29.777993265872244 24.218909063560773 14-15 19.4275246366349 35.46701929676407 25.372893936750103 19.732562129850926 16-17 24.14454591065479 26.288558842103992 25.338179631533613 24.228715615707603 18-19 19.75169120314352 31.024674083113464 25.36440027629272 23.859234437450297 20-21 24.116846934761284 31.08927929111876 25.374673984695022 19.419199789424937 22-23 23.89905293075396 26.77952225514192 29.857430080118757 19.463994733985366 24-25 19.449810641645946 30.819286478483466 25.547219443704194 24.18368343616639 26-27 19.40769938703364 26.570380011026657 34.56170815396909 19.46021244797061 28-29 19.486370261037493 26.58824418838337 34.536157646843165 19.389227903735975 30-31 19.498256604431674 26.516603434114288 34.541914157548995 19.443225803905047 32-33 19.522229114974756 26.582074033659538 34.43222563338383 19.46347121798188 34-35 19.54148928609963 26.699706125650398 34.33012043409912 19.428684154150858 36-37 19.487126046361833 26.681346107391064 34.36000276840716 19.471525077839942 38-39 19.50926348129251 26.64157940461151 34.33466574236767 19.514491371728308 40-41 19.600117429132716 26.71512649789001 34.25016410706957 19.434591965907696 42-43 19.656674833422148 26.77151588161795 34.204195414464486 19.36761387049542 44-45 19.78486154074991 26.838774741501158 34.02130733889099 19.355056378857935 46-47 19.708862736708294 26.96452033684702 33.96326414124606 19.36335278519863 48-49 19.850504090174415 26.98048467377574 33.78136954181225 19.387641694237587 50-51 19.921076854010554 27.053386442774872 33.63870747456695 19.386829228647624 52-53 20.053956008957666 27.267140047244737 33.330193703608614 19.348710240188986 54-55 20.503150720991886 27.534834222641138 32.336177199399415 19.625837856967564 56-57 21.08389107803252 28.235912561164927 30.902833830177816 19.777362530624735 58-59 21.401447409126984 28.845506563446648 30.01741999672068 19.735626030705685 60-61 21.641640392566327 29.228371259238628 29.43941597709822 19.69057237109682 62-63 21.85176844219855 29.435802008615912 29.142001048232295 19.570428500953238 64-65 22.04474111565393 29.52232309740256 29.02269111020167 19.410244676741844 66-67 22.211466677864998 29.32298224947725 29.178727499407326 19.286823573250434 68-69 22.37113901780024 29.042419563805883 29.410311511658872 19.176129906735007 70-71 22.58672705265264 28.819564189986135 29.590981228224067 19.002727529137157 72-73 22.844812857382593 28.67183707672747 29.59376003245742 18.88959003343252 74-75 23.119835344995497 28.72346713796439 29.39307851780576 18.76361899923435 76-77 23.48149729663238 28.87854518982511 28.994865132710583 18.645092380831922 78-79 23.853372097128076 29.047043682265322 28.521470766502382 18.578113454104216 80-81 24.20564299711298 29.21856546648517 28.06493246174328 18.51085907465857 82-83 24.425259094308256 29.38732974795384 27.685255932068493 18.502155225669412 84-85 24.632176613424093 29.414633247495274 27.443924677879938 18.509265461200695 86-87 24.827643177624033 29.36534053821813 27.294848702241175 18.512167581916657 88-89 25.079834534116934 29.306751871365126 27.152183272679725 18.46123032183821 90-91 25.290590714621953 29.251176113254 27.0123178198245 18.44591535229954 92-93 25.449779845545038 29.209523804623373 26.93796750460629 18.402728845225294 94-95 25.58956177779756 29.20111663389136 26.83945198830859 18.369869600002488 96-97 25.72081646246322 29.155518786742114 26.71865035958782 18.405014391206844 98-99 25.818441509581746 29.180817165324807 26.617057613397943 18.383683711695507 100 25.88162358443194 29.219870484671763 26.509591267126158 18.388914663770137 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 163451.0 1 99240.0 2 28730.0 3 21970.5 4 21331.0 5 21805.0 6 22863.0 7 22919.0 8 23019.5 9 23428.0 10 24574.5 11 36520.0 12 336210.0 13 391364.0 14 145712.0 15 140290.5 16 154416.0 17 169163.0 18 183592.0 19 198628.5 20 216203.5 21 241601.0 22 275658.0 23 313308.5 24 352325.0 25 394423.5 26 442403.0 27 501414.5 28 574019.0 29 644097.5 30 702603.0 31 767060.0 32 835551.0 33 887837.5 34 930087.0 35 987128.0 36 1052140.0 37 1117378.5 38 1192612.0 39 1235950.0 40 1249573.5 41 1286602.0 42 1338010.5 43 1345066.5 44 1327690.0 45 1344690.0 46 1369177.5 47 1383566.0 48 1382206.0 49 1364708.0 50 1346145.0 51 1323407.5 52 1289717.5 53 1223654.0 54 1130107.5 55 1037906.5 56 951368.0 57 857642.0 58 750382.5 59 630867.5 60 507878.5 61 402856.0 62 311328.0 63 228446.5 64 164765.5 65 118345.0 66 85347.0 67 63749.5 68 48136.5 69 36137.0 70 27361.0 71 21490.5 72 17623.5 73 14239.0 74 11721.5 75 10155.0 76 7953.0 77 5269.0 78 3556.0 79 2647.0 80 2069.0 81 1660.5 82 1360.5 83 1085.5 84 862.0 85 715.5 86 618.0 87 540.0 88 446.5 89 383.5 90 321.5 91 248.0 92 208.0 93 186.5 94 150.5 95 117.5 96 90.0 97 67.5 98 45.0 99 35.5 100 26.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.982020474981839E-4 2 0.0014864720764786537 3 0.00105630330836586 4 7.288970793022338E-4 5 0.0016131328804229763 6 1.7923698671366405E-4 7 4.540670330079489E-5 8 4.301687681127937E-5 9 2.8677917874186244E-5 10-11 0.005458363702053449 12-13 4.421179005603713E-5 14-15 1.3144045692335364E-5 16-17 1.9118611916124165E-5 18-19 3.584739734273281E-5 20-21 0.0012689978659327413 22-23 0.0160369306578939 24-25 0.026742158417678675 26-27 0.036804522851783775 28-29 0.04622282904696444 30-31 0.10036554308018332 32-33 0.012930156221523725 34-35 0.006836098673259147 36-37 0.001161455673904543 38-39 0.0027733936410827614 40-41 0.0022297081147179807 42-43 0.001480497510254865 44-45 0.0010300152169811893 46-47 5.73558357483725E-4 48-49 6.894649422252277E-4 50-51 0.1842090207251011 52-53 0.2979755158452427 54-55 0.15940023193744046 56-57 0.18389356362848502 58-59 0.18219678682092902 60-61 0.18020725626840733 62-63 0.18073899266232454 64-65 0.17301626836145514 66-67 0.18143562708401834 68-69 0.1623098456884256 70-71 0.1707196451050307 72-73 0.16417271543700296 74-75 0.15720159156708619 76-77 0.14991023094757433 78-79 0.14593953423524428 80-81 0.1485683433737114 82-83 0.14426546077933866 84-85 0.13395455439032397 86-87 0.13406090166910742 88-89 0.13693944767572885 90-91 0.14723123545282743 92-93 0.1424993790035867 94-95 0.14227115057383796 96-97 0.14011672199353972 98-99 0.13713182870813484 100 0.13626551660568545 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.1844042E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.466247033223354 #Duplication Level Percentage of deduplicated Percentage of total 1 69.03745654624629 23.794620318683478 2 14.449152795615404 9.960161393489408 3 5.728963152824992 5.923675778085009 4 2.9036064737113336 4.003056720408056 5 1.7510498247702713 3.017605791400732 6 1.122247551561941 2.3207796806738354 7 0.7992838975313588 1.9283821383395376 8 0.6068431971816582 1.6732486035595282 9 0.4605431333450519 1.4285874062992756 >10 2.77120556636225 17.299644755063674 >50 0.18408203449245472 4.323584622385254 >100 0.17430087885671305 12.78507163333638 >500 0.010303323668109954 2.1686969829843696 >1k 6.929778470736843E-4 0.528070361543871 >5k 1.584322532996821E-4 0.39641565524785916 >10k+ 1.1021373190459068E-4 8.448398158499671 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3041716 7.2691734703831905 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 193450 0.4623119343967775 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 67152 0.16048162842394623 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58105 0.13886086817329932 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.10739641261233798 0.0 0.0 0.0 4.540670330079489E-5 2 0.10748005653947101 0.0 0.0 0.0 4.779652979031041E-5 3 0.10755175133415648 0.0 0.0 0.0 5.257618276934145E-5 4 0.10760193769043631 1.4338958937093124E-5 0.0 0.0 5.7355835748372494E-5 5 0.10763300543480002 1.9118611916124165E-5 0.0 0.0 5.974566223788801E-5 6 0.10765690369969516 2.6288091384670725E-5 0.0 0.0 5.974566223788801E-5 7 0.10769514092352742 2.6288091384670725E-5 0.0 0.0 5.974566223788801E-5 8 0.10773576797384918 2.6288091384670725E-5 0.0 0.0 5.974566223788801E-5 9 0.10779312380959755 2.6288091384670725E-5 0.0 0.0 6.452531521691905E-5 10-11 0.10785406438508019 2.9872831118944006E-5 0.0 0.0 6.930496819595009E-5 12-13 0.10794368287843703 3.584739734273281E-5 0.0 2.3898264895155206E-6 7.52795344197389E-5 14-15 0.10806556402940232 3.943213707700609E-5 0.0 2.3898264895155206E-6 7.886427415401218E-5 16-17 0.10816832656845149 4.899144303506817E-5 0.0 2.3898264895155206E-6 8.244901388828546E-5 18-19 0.10832127546378048 5.8550748993130253E-5 0.0 4.779652979031041E-6 8.961849335683203E-5 20-21 0.10842403800282965 8.364392713304322E-5 0.0 5.9745662237888015E-6 1.0276253904916738E-4 22-23 0.10860446990278808 8.961849335683203E-5 0.0 8.364392713304323E-6 1.1829641123101827E-4 24-25 0.10931424837017417 9.081340660158979E-5 0.0 1.0754219202819842E-5 1.2188115096529154E-4 26-27 0.11199085403843156 9.678797282537858E-5 0.0 1.3144045692335364E-5 1.290506304338381E-4 28-29 0.1202022978564069 1.0037271255965186E-4 0.0 1.4338958937093124E-5 1.386099363919002E-4 30-31 0.14072493283512144 1.051523655386829E-4 0.0 1.4338958937093124E-5 1.505590688394778E-4 32-33 0.18248237108642612 1.1232184500722947E-4 0.0 1.6728785426608646E-5 1.5175398208423557E-4 34-35 0.25538880780207607 1.230760642100493E-4 0.0 1.9118611916124165E-5 1.5533872181850884E-4 36-37 0.3684933209846219 1.386099363919002E-4 0.0 2.1508438405639684E-5 1.5772854830802436E-4 38-39 0.5289175935728198 1.4099976288141572E-4 0.0 2.5093178139912965E-5 1.6011837479753987E-4 40-41 0.7366317527355507 1.4338958937093124E-4 0.0 3.2262657608459525E-5 1.648980277765709E-4 42-43 0.9942323449536734 1.5175398208423557E-4 0.0 3.3457570853217285E-5 1.7326242048987521E-4 44-45 1.3050328646549012 1.565336350632666E-4 0.0 3.3457570853217285E-5 1.768471602241485E-4 46-47 1.6757845716721151 1.6609294102132868E-4 0.0 3.584739734273281E-5 1.8999120591648387E-4 48-49 2.108380208585012 1.7087259400035972E-4 0.0 4.301687681127937E-5 2.0313525160881924E-4 50-51 2.5969587259280544 1.7923698671366403E-4 0.0 4.540670330079489E-5 2.0671999134309253E-4 52-53 3.1274834778150735 1.8282172644793734E-4 0.0 4.779652979031041E-5 2.162792973011546E-4 54-55 3.7039789320544125 1.9118611916124165E-4 0.0 4.779652979031041E-5 2.1747421054591235E-4 56-57 4.33511657406328 1.9716068538503046E-4 1.1949132447577603E-6 5.018635627982593E-5 2.198640370354279E-4 58-59 5.019229499865238 2.1269455756688132E-4 2.3898264895155206E-6 5.2576182769341456E-5 2.2703351650397446E-4 60-61 5.747833825422505 2.162792973011546E-4 2.3898264895155206E-6 5.4966009258856975E-5 2.3061825623824772E-4 62-63 6.504836459154687 2.198640370354279E-4 2.3898264895155206E-6 5.6160922503614735E-5 2.3539790921727876E-4 64-65 7.286874915191032 2.234487767697012E-4 2.3898264895155206E-6 5.7355835748372494E-5 2.4137247544106757E-4 66-67 8.100913147921991 2.2822842974873223E-4 2.3898264895155206E-6 5.7355835748372494E-5 2.4495721517534084E-4 68-69 8.947511571659355 2.3300808272776327E-4 2.3898264895155206E-6 5.7355835748372494E-5 2.545165211334029E-4 70-71 9.8218881435976 2.377877357067943E-4 2.3898264895155206E-6 6.094057548264577E-5 2.557114343781607E-4 72-73 10.710536759331234 2.521266946438874E-4 2.3898264895155206E-6 6.213548872740353E-5 2.6049108735719175E-4 74-75 11.614787357301669 2.557114343781607E-4 2.3898264895155206E-6 6.452531521691905E-5 2.676605668257383E-4 76-77 12.532382746389558 2.6049108735719175E-4 2.3898264895155206E-6 6.572022846167681E-5 2.7841478602855815E-4 78-79 13.466369955369034 2.8080461251807365E-4 2.3898264895155206E-6 6.93049681959501E-5 2.8438935225234694E-4 80-81 14.411104214071862 2.8797409198662023E-4 2.3898264895155206E-6 7.169479468546562E-5 2.8797409198662023E-4 82-83 15.365402558385732 3.0350796416847114E-4 2.3898264895155206E-6 7.169479468546562E-5 2.915588317208935E-4 84-85 16.32387473466354 3.2382148932935304E-4 2.3898264895155206E-6 7.169479468546562E-5 2.9275374496565127E-4 86-87 17.284458322644834 3.4413501449023494E-4 2.3898264895155206E-6 7.408462117498114E-5 2.951435714551668E-4 88 18.010413525538475 3.728129323644212E-4 2.3898264895155206E-6 7.408462117498114E-5 3.011181376789556E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 398010 0.0 86.57942 5 GGTATCA 398045 0.0 86.30783 8 AAGCAGT 410980 0.0 86.082054 1 TGGTATC 398275 0.0 85.77599 7 GTGGTAT 400855 0.0 85.71803 6 GCAGTGG 412965 0.0 85.21651 3 GTATCAA 403370 0.0 84.50406 9 CAGTGGT 415215 0.0 83.56172 4 AGCAGTG 427615 0.0 82.97366 2 CAACGCA 397975 0.0 45.140495 12-13 ATCAACG 398400 0.0 45.078773 10-11 ACGCAGA 404280 0.0 44.71252 14-15 AGAGTAC 401280 0.0 44.28646 18-19 AGTACTT 407030 0.0 43.524605 20-21 TCAACGC 398880 0.0 42.892616 12-13 TATCAAC 400655 0.0 42.440662 10-11 AACGCAG 400970 0.0 42.42122 14-15 GAGTACT 394795 0.0 41.727467 20-21 CGCAGAG 405645 0.0 41.695057 16-17 GCAGAGT 430510 0.0 41.436947 16-17 >>END_MODULE