FastQCFastQC Report
Thu 26 May 2016
SRR1660687_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660687_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41844042
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23131685.528070161099637No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1971290.47110410605170505No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT908890.21720893980557615No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT646540.15451184185313646No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT606650.14497882398645906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3147650.084.988038
AGTGGTA3202950.084.5736165
TGGTATC3174250.084.2042547
GTGGTAT3231400.083.561616
AAGCAGT3149900.083.371641
GCAGTGG3279950.083.208113
GTATCAA3201800.082.7256859
CAGTGGT3361900.080.866934
AGCAGTG3396500.080.417922
CAACGCA3121850.044.77626812-13
ATCAACG3119050.044.7185710-11
ACGCAGA3230900.043.88971714-15
AGAGTAC3240300.043.40737518-19
AGTACTT3285700.042.5460520-21
TCAACGC3120400.042.3318612-13
TATCAAC3175850.041.62405410-11
AACGCAG3188250.041.4750414-15
GAGTACT3155450.041.0653620-21
CGCAGAG3242200.040.74361816-17
GCAGAGT3536400.039.99595616-17
Produced by FastQC (version 0.11.5)