FastQCFastQC Report
Thu 26 May 2016
SRR1660686_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660686_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45961989
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32787437.133596851084927No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2507090.5454703015572281No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT877370.19089034636860472No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT835970.18188290328340664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA4248300.085.668985
GGTATCA4244800.085.426868
AAGCAGT4398650.085.136031
GTGGTAT4266750.085.007186
TGGTATC4252450.084.852637
GCAGTGG4427500.083.798033
GTATCAA4309950.083.604069
CAGTGGT4452450.082.2419054
AGCAGTG4557600.082.153372
CAACGCA4247050.044.90521212-13
ATCAACG4249600.044.8370610-11
ACGCAGA4307100.044.4914514-15
AGAGTAC4284350.044.0234618-19
AGTACTT4368450.042.99790220-21
TCAACGC4248050.042.55296312-13
AACGCAG4277550.042.03491614-15
TATCAAC4284600.041.97533410-11
GCAGAGT4536800.041.6645316-17
CGCAGAG4320150.041.38801216-17
GAGTACT4211150.041.37361520-21
Produced by FastQC (version 0.11.5)