FastQCFastQC Report
Thu 26 May 2016
SRR1660686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45961989
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29017856.3134452253578495No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2503350.5446565856843141No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1133870.2466973307008102No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880820.19164096662570457No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT788100.1714677752522851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3872900.085.526718
AGTGGTA3939300.085.139965
TGGTATC3905900.084.773827
GTGGTAT3954750.084.5591666
AAGCAGT3859400.084.209461
GTATCAA3925900.083.564289
GCAGTGG4062600.083.194053
CAGTGGT4132300.081.458964
AGCAGTG4155400.081.382472
CAACGCA3835850.045.08796312-13
ATCAACG3835850.044.98796510-11
ACGCAGA3961450.044.30111714-15
AGAGTAC3991150.043.73038518-19
TCAACGC3835100.042.70358712-13
AGTACTT4067150.042.69445820-21
TATCAAC3900050.042.00996810-11
AACGCAG3912900.041.88874414-15
GCAGAGT4243150.041.2565116-17
GAGTACT3904950.041.24962220-21
CGCAGAG3974300.041.2250516-17
Produced by FastQC (version 0.11.5)