##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660686_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 45961989 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.66061723307927 31.0 30.0 34.0 16.0 34.0 2 30.676138123613406 31.0 31.0 34.0 25.0 34.0 3 31.45115832563295 31.0 31.0 34.0 28.0 34.0 4 35.185968714278225 37.0 35.0 37.0 32.0 37.0 5 35.46051327326152 37.0 35.0 37.0 33.0 37.0 6 35.60743950832937 37.0 35.0 37.0 33.0 37.0 7 35.60683228917704 37.0 35.0 37.0 33.0 37.0 8 35.658041343685106 37.0 35.0 37.0 33.0 37.0 9 37.32084418713907 39.0 37.0 39.0 34.0 39.0 10-11 37.24124621760821 39.0 37.0 39.0 34.0 39.0 12-13 37.099050478429035 39.0 37.0 39.0 33.5 39.0 14-15 38.4460732323834 40.0 38.0 41.0 33.5 41.0 16-17 38.566308955863505 40.0 38.0 41.0 34.0 41.0 18-19 38.54694993726229 40.0 38.0 41.0 34.0 41.0 20-21 38.46472196840742 40.0 38.0 41.0 34.0 41.0 22-23 38.24094950503556 40.0 37.5 41.0 33.5 41.0 24-25 38.29495928690118 40.0 38.0 41.0 34.0 41.0 26-27 38.25984061525274 40.0 38.0 41.0 34.0 41.0 28-29 38.193976853351586 40.0 38.0 41.0 33.5 41.0 30-31 38.08721637351247 40.0 37.5 41.0 33.0 41.0 32-33 37.95593170478327 40.0 37.0 41.0 33.0 41.0 34-35 37.77245253681254 40.0 37.0 41.0 33.0 41.0 36-37 37.55495261530131 39.5 37.0 41.0 32.5 41.0 38-39 37.061125411696175 39.0 35.0 40.5 31.5 41.0 40-41 36.91191559399225 39.0 35.0 40.0 31.0 41.0 42-43 36.753947571764144 39.0 35.0 40.0 31.0 41.0 44-45 36.87616371432489 39.0 35.0 40.0 31.0 41.0 46-47 36.798994828095886 39.0 35.0 40.5 31.0 41.0 48-49 36.67915761870097 39.0 35.0 40.0 31.0 41.0 50-51 36.474886182580136 39.0 35.0 40.0 30.0 41.0 52-53 36.26838113990237 39.0 35.0 40.0 30.0 41.0 54-55 35.8737697252397 39.0 35.0 40.0 27.5 41.0 56-57 35.32760092257975 39.0 34.5 40.0 24.5 41.0 58-59 35.08257371107243 38.0 34.0 40.0 24.5 41.0 60-61 34.89905969909179 38.0 34.0 40.0 24.5 41.0 62-63 34.59587423425039 37.5 34.0 40.0 23.5 41.0 64-65 34.33121854234811 37.0 34.0 40.0 23.0 41.0 66-67 34.129757123870334 36.5 33.0 40.0 23.0 41.0 68-69 33.87925711831139 36.0 33.0 39.0 23.0 41.0 70-71 33.596338346018925 36.0 33.0 39.0 23.0 41.0 72-73 33.26754677653311 35.0 33.0 38.5 22.5 40.0 74-75 32.90121518674921 35.0 32.5 37.5 21.5 40.0 76-77 31.12625756905342 33.5 30.0 36.0 19.5 38.5 78-79 32.146221021897034 35.0 32.0 36.0 20.0 39.0 80-81 32.09400569022372 35.0 32.0 36.0 20.0 39.0 82-83 31.84615964944424 35.0 32.0 36.0 19.5 37.5 84-85 31.60051448165135 35.0 32.0 35.5 19.0 37.0 86-87 31.36702993858686 35.0 32.0 35.0 19.0 37.0 88-89 31.158850969221547 35.0 32.0 35.0 18.0 36.0 90-91 30.972345692002143 35.0 31.0 35.0 18.0 36.0 92-93 30.779376442999453 34.5 31.0 35.0 17.0 36.0 94-95 30.618143570766705 34.0 31.0 35.0 15.5 35.0 96-97 30.492565944002116 34.0 31.0 35.0 15.0 35.0 98-99 30.369586616453873 34.0 31.0 35.0 14.0 35.0 100 30.285747142056884 34.0 31.0 35.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.7736062431195982 1101 2 2.0016850850436114 1101 3 1.301378949761098 1101 4 1.0818824681566 1101 5 0.6538959357966476 1101 6 0.43935151802194383 1101 7 0.38522497768411057 1101 8 0.36242745864038994 1101 9 0.3935941028277625 1101 10-11 0.3669020802401306 1101 12-13 0.610108943087333 1101 14-15 0.6454941055513785 1101 16-17 0.4553112326628721 1101 18-19 0.4485442936227173 1101 20-21 0.4289927548600687 1101 22-23 0.4186657562446072 1101 24-25 0.4255735392125999 1101 26-27 0.40054219115133094 1101 28-29 0.38922373522964193 1101 30-31 0.35560815548699765 1101 32-33 0.3495588681027897 1101 34-35 0.3172509181651222 1101 36-37 0.29906693324073075 1101 38-39 0.3807249682040563 1101 40-41 0.37198159557141963 1101 42-43 0.3637973119456177 1101 44-45 0.25899492011035363 1101 46-47 0.22033634806514613 1101 48-49 0.17991549785886463 1101 50-51 0.24913303276918697 1101 52-53 0.3033778870548005 1101 54-55 0.31073075829534247 1101 56-57 0.19032572805043202 1101 58-59 0.17740133417697024 1101 60-61 0.208924034021301 1101 62-63 0.2653023772519063 1101 64-65 0.29246655247322195 1101 66-67 0.23867601447285125 1101 68-69 0.16368108950200622 1101 70-71 0.10415775512758785 1101 72-73 0.09970716391077161 1101 74-75 0.05840229279021969 1101 76-77 0.09584537476111521 1101 78-79 0.07076621586252685 1101 80-81 0.09137972760388635 1101 82-83 0.17254502545192096 1101 84-85 0.0871609394234909 1101 86-87 0.0989256235403353 1101 88-89 -0.009450883676120014 1101 90-91 0.0017639258044503947 1101 92-93 0.07380659796690026 1101 94-95 0.060155906299929285 1101 96-97 0.06992753673303298 1101 98-99 0.06931346877966149 1101 100 0.08564903774588473 1102 1 2.7377085379586994 1102 2 1.9802813265628814 1102 3 1.2782233947238772 1102 4 1.0490585806756059 1102 5 0.6323802931891933 1102 6 0.44283441635427323 1102 7 0.338047461201576 1102 8 0.30952129792679983 1102 9 0.3067917875081321 1102 10-11 0.27201503841824604 1102 12-13 0.522443450713233 1102 14-15 0.5881139432076878 1102 16-17 0.3218990070757215 1102 18-19 0.3616020755195706 1102 20-21 0.3566403662687847 1102 22-23 0.3454917179637036 1102 24-25 0.3368409952197524 1102 26-27 0.30182756196862925 1102 28-29 0.26416998750872267 1102 30-31 0.27152766325047395 1102 32-33 0.295991330423405 1102 34-35 0.26534896742722225 1102 36-37 0.34110329698469144 1102 38-39 0.3878006168257784 1102 40-41 0.3827659281044049 1102 42-43 0.3205605259853854 1102 44-45 0.31021644309588225 1102 46-47 0.3070892104592531 1102 48-49 0.21677057141041445 1102 50-51 0.26638585462134046 1102 52-53 0.24482912497541776 1102 54-55 0.3600224219805952 1102 56-57 0.3435547135370527 1102 58-59 0.34886767081638226 1102 60-61 0.2913089528146955 1102 62-63 0.29282660696620866 1102 64-65 0.2548016410017695 1102 66-67 0.22579234305408846 1102 68-69 0.2159776069851702 1102 70-71 0.23425602757703246 1102 72-73 0.1896162161291528 1102 74-75 0.16036308738330263 1102 76-77 0.15400063665031283 1102 78-79 0.2637437433366827 1102 80-81 0.29283441521540254 1102 82-83 0.2696112253506051 1102 84-85 0.22334769684503186 1102 86-87 0.2311722763696089 1102 88-89 0.18298190679016457 1102 90-91 0.11954920307613648 1102 92-93 0.2516516682807861 1102 94-95 0.1849911834115474 1102 96-97 0.20376978515642818 1102 98-99 0.14158586623472047 1102 100 0.035609518305172116 1103 1 2.7530436318454896 1103 2 1.9668443874840946 1103 3 1.2454808311767316 1103 4 1.0484118494193524 1103 5 0.6222144843030506 1103 6 0.4007184180915502 1103 7 0.2970233605641681 1103 8 0.28048648856851344 1103 9 0.29715452256289865 1103 10-11 0.2961470377718811 1103 12-13 0.5558610147988645 1103 14-15 0.587460909987648 1103 16-17 0.3426382609938017 1103 18-19 0.25980993997232105 1103 20-21 0.2608095503651242 1103 22-23 0.29990753998353625 1103 24-25 0.32838754831774253 1103 26-27 0.3259814056063135 1103 28-29 0.33065847833221085 1103 30-31 0.3052088891032767 1103 32-33 0.3106529676167824 1103 34-35 0.3221525811231274 1103 36-37 0.2619255998781327 1103 38-39 0.3069505623693871 1103 40-41 0.30218833284779834 1103 42-43 0.26797713242616084 1103 44-45 0.3067807844455359 1103 46-47 0.19182778606786144 1103 48-49 0.16868358252725812 1103 50-51 0.21902612773960328 1103 52-53 0.18457312823228023 1103 54-55 0.29343324761424583 1103 56-57 0.29894980347255995 1103 58-59 0.2689692299287998 1103 60-61 0.27791079796363505 1103 62-63 0.2918668082974989 1103 64-65 0.27197062220587753 1103 66-67 0.16460094725608343 1103 68-69 0.18730924682510874 1103 70-71 0.06147694611468779 1103 72-73 0.06381069064622835 1103 74-75 0.0872079389346041 1103 76-77 -0.02992075310331188 1103 78-79 0.19480169724817387 1103 80-81 0.19301098405583872 1103 82-83 0.20994142528396864 1103 84-85 0.20501280407122024 1103 86-87 0.20658616462658586 1103 88-89 0.19166587188552242 1103 90-91 0.19709483470517242 1103 92-93 0.18125402826337478 1103 94-95 0.158693964635237 1103 96-97 0.17279795915609597 1103 98-99 0.09880894682744668 1103 100 0.13796131863433914 1104 1 2.684500087102567 1104 2 1.9324652445976511 1104 3 1.246586736015825 1104 4 0.9985586295687767 1104 5 0.5661245797217447 1104 6 0.3881797941158922 1104 7 0.32394718687177004 1104 8 0.3015255237065233 1104 9 0.31737312000573326 1104 10-11 0.24502058586126196 1104 12-13 0.5083455427346664 1104 14-15 0.5035577615882332 1104 16-17 0.3038582368868603 1104 18-19 0.2725343280219903 1104 20-21 0.29943586643174314 1104 22-23 0.3089142136988201 1104 24-25 0.28056896508112317 1104 26-27 0.25578257550711925 1104 28-29 0.23486239687143495 1104 30-31 0.23550657790404017 1104 32-33 0.2476699062000023 1104 34-35 0.27496927105435276 1104 36-37 0.21469383506406103 1104 38-39 0.26129981231218835 1104 40-41 0.29208816758314526 1104 42-43 0.26946887341546244 1104 44-45 0.20934241211139692 1104 46-47 0.23543552783660004 1104 48-49 0.2541623803526605 1104 50-51 0.20900607604861676 1104 52-53 0.2490101011923187 1104 54-55 0.23562563866018849 1104 56-57 0.19339370091564234 1104 58-59 0.09425935605560909 1104 60-61 0.09928636018653236 1104 62-63 0.1688683148032908 1104 64-65 0.14073924338384103 1104 66-67 0.10498009437787204 1104 68-69 0.10697717261817274 1104 70-71 0.11218657419459532 1104 72-73 0.059448150276473655 1104 74-75 0.024374348852326477 1104 76-77 0.026912065024795595 1104 78-79 0.12359621900528595 1104 80-81 0.13036672088648515 1104 82-83 0.15859449677089899 1104 84-85 0.08474585425465975 1104 86-87 0.05511523912263527 1104 88-89 0.0847217791422672 1104 90-91 0.11770965722291393 1104 92-93 0.17756570689693518 1104 94-95 0.15617907913205542 1104 96-97 0.11409854402148412 1104 98-99 0.12574688853911908 1104 100 0.13541869424132358 1105 1 2.6515711359291423 1105 2 1.928755372276818 1105 3 1.1921553741018407 1105 4 0.9940288844021694 1105 5 0.5661648496955962 1105 6 0.35803820060927904 1105 7 0.26043979643041126 1105 8 0.2364336520201178 1105 9 0.29001726208293377 1105 10-11 0.24647603706359433 1105 12-13 0.4306982117754856 1105 14-15 0.5043762435174202 1105 16-17 0.29534169182431214 1105 18-19 0.2518085365393148 1105 20-21 0.2818660803654538 1105 22-23 0.24824460850731356 1105 24-25 0.23382205490829477 1105 26-27 0.2512463929434503 1105 28-29 0.2274117601681951 1105 30-31 0.1782575030379192 1105 32-33 0.20630997288375852 1105 34-35 0.28564558438188925 1105 36-37 0.292115424178661 1105 38-39 0.2872645675358285 1105 40-41 0.22115219528217978 1105 42-43 0.23343572357106268 1105 44-45 0.225028456330584 1105 46-47 0.19134594030167307 1105 48-49 0.21913011841455443 1105 50-51 0.2672324010751268 1105 52-53 0.26759255681614746 1105 54-55 0.25014105613212934 1105 56-57 0.29127142980289733 1105 58-59 0.22604759028197918 1105 60-61 0.23191620865632245 1105 62-63 0.31311861119193907 1105 64-65 0.26567734073893234 1105 66-67 0.18615745801983508 1105 68-69 0.17627056254136164 1105 70-71 0.144151238158436 1105 72-73 0.11163880433411322 1105 74-75 0.10864255248887389 1105 76-77 0.13102626621971325 1105 78-79 0.24195649940812558 1105 80-81 0.2470967079534674 1105 82-83 0.19848434291601436 1105 84-85 0.15852478562339556 1105 86-87 0.17985791073770585 1105 88-89 0.20696313998496763 1105 90-91 0.18694507922791104 1105 92-93 0.216168038366213 1105 94-95 0.22284894177242265 1105 96-97 0.24781530369110172 1105 98-99 0.22226133305902707 1105 100 0.24961653528644945 1106 1 2.594901676312343 1106 2 1.8631163286909 1106 3 1.1640755124319462 1106 4 0.9823251845437753 1106 5 0.5609048268045953 1106 6 0.3996277185483663 1106 7 0.25603708890463395 1106 8 0.26754509821768835 1106 9 0.27241064955158834 1106 10-11 0.23382348691967536 1106 12-13 0.47796867534225385 1106 14-15 0.4758821736968315 1106 16-17 0.3264185976721947 1106 18-19 0.2575450801239043 1106 20-21 0.25520599061496796 1106 22-23 0.24170065580930356 1106 24-25 0.24589141609509824 1106 26-27 0.21037937681024488 1106 28-29 0.23285713500190752 1106 30-31 0.2652689786826059 1106 32-33 0.27858889938765685 1106 34-35 0.24412582717204856 1106 36-37 0.31146712635041496 1106 38-39 0.2713809168887096 1106 40-41 0.34081192287862194 1106 42-43 0.3572987353631518 1106 44-45 0.3000726395543154 1106 46-47 0.26970949745251005 1106 48-49 0.28675358434247045 1106 50-51 0.2998872371530297 1106 52-53 0.3005837751233642 1106 54-55 0.2015337017291543 1106 56-57 0.2388951714737928 1106 58-59 0.2837985457151291 1106 60-61 0.2541789914148538 1106 62-63 0.28011226458340843 1106 64-65 0.24654916608879063 1106 66-67 0.14087212203457256 1106 68-69 0.18733244969941154 1106 70-71 0.18717290468671166 1106 72-73 0.17882775386049587 1106 74-75 0.22035235080981153 1106 76-77 0.13601746113742763 1106 78-79 0.25001561160815555 1106 80-81 0.2679413076297621 1106 82-83 0.24846509044462906 1106 84-85 0.24072363627130855 1106 86-87 0.21455663427234484 1106 88-89 0.25059756826169277 1106 90-91 0.2388581382642414 1106 92-93 0.25226431783037384 1106 94-95 0.28237398548788306 1106 96-97 0.30777869630269805 1106 98-99 0.27194671695369266 1106 100 0.2997932209133083 1107 1 0.6489916078149385 1107 2 0.9688267711296064 1107 3 0.7525045840013043 1107 4 0.7584376710323468 1107 5 0.4291756910124036 1107 6 0.307407540687187 1107 7 0.20258615820502257 1107 8 0.21086706855401616 1107 9 0.2725333185609955 1107 10-11 0.15239498044774535 1107 12-13 0.4558200495861513 1107 14-15 0.4260139675472203 1107 16-17 0.27005155232031797 1107 18-19 0.22852360515292247 1107 20-21 0.250505316979563 1107 22-23 0.22667158810956067 1107 24-25 0.2408872267103419 1107 26-27 0.17924858457330828 1107 28-29 0.20714547357459878 1107 30-31 0.1633214667869538 1107 32-33 0.2500746139835286 1107 34-35 0.17393759213624804 1107 36-37 0.22434034966880745 1107 38-39 0.26657169801679714 1107 40-41 0.20446576765360192 1107 42-43 0.2580663923812807 1107 44-45 0.24938884442840958 1107 46-47 0.2407388950713738 1107 48-49 0.2397491563469174 1107 50-51 0.21197877744125293 1107 52-53 0.2613680910096363 1107 54-55 0.23597157045610828 1107 56-57 0.21905198078822963 1107 58-59 0.23493168670059106 1107 60-61 0.21790916784511438 1107 62-63 0.24690733813745425 1107 64-65 0.20784804994063677 1107 66-67 0.1847671257978334 1107 68-69 0.20028872103625872 1107 70-71 0.14518865531972835 1107 72-73 0.11936432107535921 1107 74-75 0.06538794514903401 1107 76-77 0.029987671492090584 1107 78-79 0.21785441759971036 1107 80-81 0.241366831258361 1107 82-83 0.17924320941519412 1107 84-85 0.17902227350072408 1107 86-87 0.17913952626516405 1107 88-89 0.24120544983322745 1107 90-91 0.2601244858154992 1107 92-93 0.23090860612428088 1107 94-95 0.22757906627077418 1107 96-97 0.27581190698793634 1107 98-99 0.25429866917987454 1107 100 0.24412644136791073 1108 1 0.8681482715280318 1108 2 1.0625122923829124 1108 3 0.7679937544670743 1108 4 0.7751787528829936 1108 5 0.3990240046966065 1108 6 0.24574723924340702 1108 7 0.15829353854917372 1108 8 0.1692833335979742 1108 9 0.16051872157871827 1108 10-11 0.09449229521989366 1108 12-13 0.37489342263614844 1108 14-15 0.35894169122958175 1108 16-17 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-1.3161037294009539 2316 100 -1.2407019579999243 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 42.0 7 815.0 8 1032.0 9 451.0 10 267.0 11 265.0 12 547.0 13 2419.0 14 10381.0 15 28221.0 16 51658.0 17 75082.0 18 95403.0 19 124766.0 20 168849.0 21 233199.0 22 340844.0 23 518803.0 24 766992.0 25 939255.0 26 924666.0 27 818575.0 28 789673.0 29 869164.0 30 1022125.0 31 1243574.0 32 1538001.0 33 1948056.0 34 2596634.0 35 3812093.0 36 5731919.0 37 9107915.0 38 9829226.0 39 2367810.0 40 3267.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.821528652797095 35.80350455387941 27.63964708756327 18.73531970576023 2 20.324332984835003 35.39644011272713 26.649332912492575 17.629893989945295 3 27.247937420636863 26.607599597136666 26.56772751936388 19.576735462862587 4 19.806400445794488 27.462235857258214 25.589285893781877 27.142077803165428 5 20.143656098085746 34.54783255789909 26.00775392901295 19.30075741500221 6 27.967473296249214 26.966757248038153 26.020790788666698 19.044978667045935 7 19.938332085671924 26.63547262935031 33.95378298358672 19.47241230139105 8 27.60418397036734 27.00502582688491 25.952432128209246 19.438358074538506 9 27.075051081884205 26.53953030622761 26.78452840672322 19.600890205164966 10-11 19.30541561201801 31.18055661168188 30.11454856751304 19.39947920878707 12-13 19.27852218063119 27.386606733640807 29.846617389784353 23.488253695943648 14-15 19.256506719063008 35.09596049030863 25.97546529154776 19.672067499080597 16-17 23.360257038562857 27.124921198447193 25.992233797612048 23.5225879653779 18-19 19.58554274054589 31.198519280790915 26.017961276654063 23.197976702009132 20-21 23.366905422652618 31.229591695868514 26.02630404006232 19.377198841416547 22-23 23.12915855212565 27.526100890484813 29.96326931959891 19.381471237790628 24-25 19.303288309820022 30.98801485839035 26.16503208852722 23.54366474326241 26-27 19.346441374016912 27.21282033033965 34.046225654354615 19.39451264128883 28-29 19.352414827166406 27.33353988083848 33.97235780830615 19.341687483688965 30-31 19.408536447990578 27.279181437108026 33.94503968001214 19.36724243488926 32-33 19.381857624136646 27.39429358468807 33.80990793880184 19.41394085237344 34-35 19.441881849824664 27.42377214597667 33.754894003334954 19.379452000863715 36-37 19.437042857305414 27.445983680123152 33.74719814671206 19.36977531585937 38-39 19.467801497703523 27.421994363296072 33.76509321282965 19.34511092617076 40-41 19.458175389824675 27.533045712253042 33.71175301828023 19.297025879642053 42-43 19.48940405543065 27.540160137633997 33.63420644453116 19.336229362404193 44-45 19.502123394109127 27.608559700754824 33.58529123805678 19.304025667079266 46-47 19.56303187621086 27.727794258285236 33.45572906836246 19.253444797141448 48-49 19.580982404918913 27.792578331468665 33.31890761644127 19.307531647171153 50-51 19.637607051944062 27.862681608628666 33.13413831587523 19.36557302355205 52-53 19.736665605775624 28.016383817471397 32.85439742239501 19.39255315435797 54-55 20.030723461341623 28.323660066217364 32.026061605821376 19.619554866619634 56-57 20.47441936427143 28.95465867029337 30.73361291206863 19.83730905336657 58-59 20.63237924337159 29.49887715096172 29.974455376227493 19.894288229439198 60-61 20.700200637672054 29.785730050740312 29.559246692168994 19.954822619418643 62-63 20.723903168806494 29.969969249775747 29.333940932705588 19.97218664871217 64-65 20.71129008350688 30.167711151823625 29.133753967736574 19.98724479693292 66-67 20.649821932859698 30.079797870244263 29.213257364954032 20.05712283194201 68-69 20.586285046518757 29.91010552415413 29.392090656485763 20.111518772841354 70-71 20.53621173052998 29.780693391501128 29.523978995030625 20.15911588293827 72-73 20.529253703143866 29.690147071298334 29.519278810234766 20.261320415323034 74-75 20.56705122895332 29.736307917017925 29.33354286315429 20.363097990874465 76-77 20.588174947076958 29.843381779660287 29.15664039195765 20.411802881305103 78-79 20.706515494553994 30.01626991277159 28.730913073641457 20.54630151903296 80-81 20.795502996425498 30.207195480395487 28.324740104708034 20.67256141847098 82-83 20.846923837528593 30.350793337604713 27.959445784199122 20.84283704066757 84-85 20.886411166905965 30.371501354681552 27.737699328456962 21.004388149955517 86-87 20.92941234957824 30.340766774161544 27.60639492006709 21.123425956193127 88-89 21.001714442323767 30.310384690938307 27.49066084913246 21.197240017605463 90-91 21.112028811845377 30.20358483085328 27.37602263139439 21.30836372590695 92-93 21.16992317998522 30.11062797516742 27.345380065028515 21.37406877981885 94-95 21.256757147528702 30.022203303397397 27.32504721008826 21.395992338985632 96-97 21.25963552578051 29.907212919551174 27.327378860876472 21.50577269379184 98-99 21.218515892044998 29.822776786350204 27.371887535049304 21.586819786555495 100 21.191254364557636 29.80105147320757 27.326395731046365 21.681298431188434 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 247003.0 1 152758.0 2 45148.5 3 29438.5 4 26831.5 5 26959.5 6 27980.5 7 28132.0 8 27913.0 9 28389.0 10 30219.5 11 47991.0 12 367510.5 13 415750.0 14 149911.5 15 147465.0 16 166144.0 17 185929.0 18 204082.0 19 221904.0 20 243441.0 21 274808.0 22 317640.0 23 366230.0 24 417082.0 25 472905.0 26 536165.5 27 611624.0 28 703760.0 29 794709.5 30 869097.5 31 939525.0 32 1011095.0 33 1071874.0 34 1120509.0 35 1173322.5 36 1231934.5 37 1290652.0 38 1352629.5 39 1390606.5 40 1400632.5 41 1418932.0 42 1451823.5 43 1460224.0 44 1453733.0 45 1464621.0 46 1472208.5 47 1467180.0 48 1447923.0 49 1426448.5 50 1412495.5 51 1388882.5 52 1343749.5 53 1261909.0 54 1154684.5 55 1052575.0 56 954144.0 57 849511.0 58 738142.5 59 621067.0 60 504106.0 61 400790.5 62 310258.5 63 228500.5 64 164898.5 65 120020.0 66 86870.0 67 63834.0 68 47151.0 69 35168.5 70 26763.5 71 20824.0 72 16849.0 73 13332.0 74 10583.0 75 9015.0 76 6844.5 77 4074.5 78 2410.0 79 1643.5 80 1121.0 81 786.5 82 533.0 83 358.0 84 255.5 85 167.5 86 108.0 87 80.5 88 56.0 89 42.0 90 29.0 91 20.5 92 14.5 93 6.5 94 4.0 95 4.0 96 3.5 97 2.0 98 1.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20441674097263282 2 6.0919904924044956E-5 3 0.0 4 6.5271326704333885E-6 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.3935567279301163 14-15 0.0 16-17 3.0459952462022478E-5 18-19 0.0 20-21 0.0 22-23 7.6149881155056195E-6 24-25 1.7188116032141256E-4 26-27 4.786563958317818E-5 28-29 8.70284356057785E-6 30-31 2.621731622624077E-4 32-33 6.091990492404495E-4 34-35 1.0878554450722312E-6 36-37 0.0 38-39 4.786563958317818E-5 40-41 2.7196386126805787E-5 42-43 1.9255041377778495E-4 44-45 1.707933048763403E-4 46-47 6.179018928010275E-4 48-49 0.0027109357691200006 50-51 0.018195470174278142 52-53 0.04579327496031558 54-55 0.05887582454275423 56-57 0.059103186330774334 58-59 0.05934142667324514 60-61 0.05212133008430075 62-63 0.02557548151364816 64-65 0.011002569971460548 66-67 0.009102086508919359 68-69 0.007113486755327321 70-71 0.008511381002245137 72-73 0.010124670627287257 74-75 0.004229581970440836 76-77 0.005069406374036598 78-79 0.019731522062720133 80-81 0.036977294433450214 82-83 0.04648297531249137 84-85 0.04715200641121079 86-87 0.04206737006094318 88-89 0.024973897452523215 90-91 0.0076813472976550255 92-93 0.0020016540189329054 94-95 0.006662026745622345 96-97 0.004895349502825041 98-99 0.0010606590589454256 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.5961989E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.10748261443226 #Duplication Level Percentage of deduplicated Percentage of total 1 70.1332030373581 26.024666124032137 2 14.465138309450262 10.735297366665671 3 5.4442790611806435 6.06070471832635 4 2.695626504525371 4.00111654606712 5 1.5529262074322645 2.8812591121894484 6 1.0215669647706462 2.2744667030823047 7 0.7000062541058159 1.8182828932957789 8 0.5455522348240636 1.6195256055198881 9 0.4268661379153387 1.4255935012244945 >10 2.665311938078201 17.972703707289064 >50 0.18392882552641804 4.714094986824537 >100 0.16338461664088577 11.54130575985906 >500 0.0015179847508526986 0.3424111498757107 >1k 4.814016850291426E-4 0.4464669080177098 >5k 1.169565429276543E-4 0.3065185464985743 >10k+ 9.356521300989262E-5 7.835586371231994 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2901785 6.3134452253578495 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 250335 0.5446565856843141 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 113387 0.2466973307008102 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 88082 0.19164096662570457 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 78810 0.1714677752522851 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.06145948122480078 4.351421780288926E-6 2.175710890144463E-6 0.0 2.8284241571878013E-5 2 0.06154433394951642 8.702843560577851E-6 2.175710890144463E-6 2.175710890144463E-6 3.0459952462022478E-5 3 0.061594375299989734 1.7405687121155703E-5 2.175710890144463E-6 2.175710890144463E-6 3.263566335216694E-5 4 0.06162483525245176 1.9581398011300165E-5 4.351421780288926E-6 2.175710890144463E-6 3.263566335216694E-5 5 0.06164006522868277 2.393281979158909E-5 4.351421780288926E-6 2.175710890144463E-6 3.263566335216694E-5 6 0.061650943783133495 3.916279602260033E-5 4.351421780288926E-6 4.351421780288926E-6 3.6987085132455864E-5 7 0.061672700892034936 4.133850691274479E-5 4.351421780288926E-6 6.527132670433388E-6 3.916279602260033E-5 8 0.061698809422716674 4.568992869303371E-5 4.351421780288926E-6 6.527132670433388E-6 4.351421780288925E-5 9 0.06172274224250826 5.004135047332264E-5 4.351421780288926E-6 6.527132670433388E-6 4.351421780288925E-5 10-11 0.061764080749421 6.200776036911719E-5 4.351421780288926E-6 6.527132670433388E-6 4.568992869303371E-5 12-13 0.06180650711177882 7.506202570998395E-5 4.351421780288926E-6 6.527132670433388E-6 5.656848314375603E-5 14-15 0.061876129860263446 8.267701382548958E-5 4.351421780288926E-6 8.702843560577851E-6 6.091990492404495E-5 16-17 0.06194140118696778 8.811629105085073E-5 4.351421780288926E-6 8.702843560577851E-6 6.418347125926164E-5 18-19 0.06204692316513978 1.0660983361707867E-4 4.351421780288926E-6 8.702843560577851E-6 6.853489303955057E-5 20-21 0.06209587666016804 1.2183980984808991E-4 4.351421780288926E-6 1.0878554450722313E-5 6.962274848462281E-5 22-23 0.062219892180906275 1.3163050885374E-4 4.351421780288926E-6 1.1966409895794544E-5 6.962274848462281E-5 24-25 0.06275729277077195 1.4794834052982348E-4 4.351421780288926E-6 1.5229976231011239E-5 6.962274848462281E-5 26-27 0.06464580982341735 1.5882689498054577E-4 4.351421780288926E-6 2.284496434651686E-5 6.962274848462281E-5 28-29 0.070273286040776 1.642661722059069E-4 4.351421780288926E-6 2.8284241571878013E-5 6.962274848462281E-5 30-31 0.08451984094944194 1.7949614843691816E-4 4.351421780288926E-6 2.8284241571878013E-5 7.397417026491173E-5 32-33 0.11361671053878891 1.903747028876405E-4 4.351421780288926E-6 2.8284241571878013E-5 7.723773660012843E-5 34-35 0.16617644636745377 1.979896910031461E-4 4.351421780288926E-6 3.263566335216694E-5 8.050130293534512E-5 36-37 0.24886760231372929 2.0342896822850726E-4 4.351421780288926E-6 3.263566335216694E-5 8.376486927056182E-5 38-39 0.3662689619459245 2.153953781243018E-4 4.351421780288926E-6 3.4811374242311405E-5 8.920414649592297E-5 40-41 0.5213917091359993 2.2844964346516858E-4 4.351421780288926E-6 3.6987085132455864E-5 9.137985738606744E-5 42-43 0.7176506221260355 2.4476747514125204E-4 4.351421780288926E-6 3.80749405775281E-5 9.573127916635635E-5 44-45 0.9586726109699039 2.5238246325675766E-4 4.351421780288926E-6 3.916279602260033E-5 9.573127916635635E-5 46-47 1.247389446092074 2.6652458404269666E-4 4.351421780288926E-6 3.916279602260033E-5 9.573127916635635E-5 48-49 1.5857473008837804 2.839302711638524E-4 4.351421780288926E-6 3.916279602260033E-5 9.681913461142858E-5 50-51 1.9692130817054068 2.8719383749906905E-4 4.351421780288926E-6 4.025065146767256E-5 1.0008270094664527E-4 52-53 2.3929708525016182 3.0024810283993587E-4 4.351421780288926E-6 4.242636235781702E-5 1.0225841183678974E-4 54-55 2.8595118022416304 3.0568738006529696E-4 4.351421780288926E-6 4.351421780288925E-5 1.0334626728186196E-4 56-57 3.371745944241012 3.1656593451601935E-4 4.351421780288926E-6 4.568992869303371E-5 1.0660983361707867E-4 58-59 3.9265282884080586 3.2526877807659716E-4 4.351421780288926E-6 4.568992869303371E-5 1.1096125539736759E-4 60-61 4.521098945478622 3.3397162163717497E-4 4.351421780288926E-6 4.568992869303371E-5 1.1640053262272876E-4 62-63 5.143805895780533 3.4158660975268064E-4 4.351421780288926E-6 4.895349502825041E-5 1.1966409895794545E-4 64-65 5.7980378090251925 3.492015978681862E-4 4.351421780288926E-6 5.004135047332264E-5 1.2292766529316214E-4 66-67 6.483036449967385 3.5572873053861965E-4 4.351421780288926E-6 5.22170613634671E-5 1.2619123162837886E-4 68-69 7.2014311652178495 3.6334371865412527E-4 4.351421780288926E-6 5.22170613634671E-5 1.3489407518895666E-4 70-71 7.944261289475527 3.687829958794864E-4 4.351421780288926E-6 5.22170613634671E-5 1.4142120785939007E-4 72-73 8.7060668762616 3.818372612203532E-4 4.351421780288926E-6 5.439277225361157E-5 1.4142120785939007E-4 74-75 9.488565649323835 3.9815509289643666E-4 4.351421780288926E-6 5.983204947897272E-5 1.45772629639679E-4 76-77 10.285616664674803 4.112093582373035E-4 4.351421780288926E-6 6.0919904924044956E-5 1.4794834052982346E-4 78-79 11.100457380119037 4.2317576813309795E-4 4.351421780288926E-6 6.0919904924044956E-5 1.5121190686504015E-4 80-81 11.933927620060132 4.307907562486036E-4 4.351421780288926E-6 6.200776036911719E-5 1.5447547320025684E-4 82-83 12.776121372815263 4.3514217802889253E-4 4.351421780288926E-6 6.527132670433388E-5 1.5882689498054577E-4 84-85 13.629270917757715 4.4275716614439816E-4 4.351421780288926E-6 6.744703759447835E-5 1.631783167608347E-4 86-87 14.485957080752097 4.5689928693033716E-4 4.351421780288926E-6 6.853489303955057E-5 1.664418830960514E-4 88 15.132195432186366 4.65602130490915E-4 4.351421780288926E-6 6.962274848462281E-5 1.6752973854112363E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 387290 0.0 85.52671 8 AGTGGTA 393930 0.0 85.13996 5 TGGTATC 390590 0.0 84.77382 7 GTGGTAT 395475 0.0 84.559166 6 AAGCAGT 385940 0.0 84.20946 1 GTATCAA 392590 0.0 83.56428 9 GCAGTGG 406260 0.0 83.19405 3 CAGTGGT 413230 0.0 81.45896 4 AGCAGTG 415540 0.0 81.38247 2 CAACGCA 383585 0.0 45.087963 12-13 ATCAACG 383585 0.0 44.987965 10-11 ACGCAGA 396145 0.0 44.301117 14-15 AGAGTAC 399115 0.0 43.730385 18-19 TCAACGC 383510 0.0 42.703587 12-13 AGTACTT 406715 0.0 42.694458 20-21 TATCAAC 390005 0.0 42.009968 10-11 AACGCAG 391290 0.0 41.888744 14-15 GCAGAGT 424315 0.0 41.25651 16-17 GAGTACT 390495 0.0 41.249622 20-21 CGCAGAG 397430 0.0 41.22505 16-17 >>END_MODULE