FastQCFastQC Report
Thu 26 May 2016
SRR1660685_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660685_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38515382
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23911026.208174178306216No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1961380.5092458903821855No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750220.19478451492445278No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT426430.11071680400313828No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC405060.10516837143144522Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2986100.087.7042548
GTGGTAT2994550.087.265546
AGTGGTA3012500.086.948925
TGGTATC2999200.086.779667
GCAGTGG3082550.086.609173
GTATCAA3024950.086.1278469
AAGCAGT3202000.083.718151
CAGTGGT3227650.081.644594
AGCAGTG3331700.080.647742
CAACGCA2971300.045.6774612-13
ATCAACG2972050.045.65631510-11
ACGCAGA3021350.045.081514-15
AGAGTAC3005250.044.49296618-19
TCAACGC2977150.044.08372512-13
AACGCAG2991500.043.65406814-15
TATCAAC2995450.043.39894510-11
CGCAGAG3024350.042.92864216-17
AGTACTT3136600.042.6703320-21
GAGTACT2989600.042.39779320-21
GCAGAGT3264500.041.09500516-17
Produced by FastQC (version 0.11.5)