##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660685_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38515382 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.226367013573952 31.0 31.0 34.0 28.0 34.0 2 31.363116585472266 31.0 31.0 34.0 28.0 34.0 3 31.41540216321884 33.0 31.0 34.0 28.0 34.0 4 34.95406295074524 37.0 35.0 37.0 32.0 37.0 5 34.92101856863318 37.0 35.0 37.0 32.0 37.0 6 34.96754359076589 37.0 35.0 37.0 32.0 37.0 7 34.87442842965961 37.0 35.0 37.0 32.0 37.0 8 34.83368377340773 37.0 35.0 37.0 32.0 37.0 9 36.42656315858428 39.0 37.0 39.0 32.0 39.0 10-11 36.336972420525385 39.0 36.0 39.0 32.0 39.0 12-13 36.395088149456754 39.0 37.0 39.0 32.0 39.0 14-15 37.649610745130346 40.0 37.0 41.0 32.0 41.0 16-17 37.5383301533917 40.0 37.0 41.0 31.5 41.0 18-19 37.47251055435462 40.0 37.0 41.0 31.0 41.0 20-21 37.37184174883687 40.0 37.0 41.0 31.0 41.0 22-23 37.18883577475617 39.5 36.5 41.0 31.0 41.0 24-25 37.21977493303844 40.0 37.0 41.0 31.0 41.0 26-27 37.23352469930066 40.0 36.5 41.0 31.0 41.0 28-29 37.122109161477354 40.0 36.0 41.0 30.5 41.0 30-31 36.939887523379625 40.0 36.0 41.0 30.0 41.0 32-33 36.832721936913416 39.5 36.0 41.0 30.0 41.0 34-35 36.690334565550984 39.0 36.0 41.0 30.0 41.0 36-37 36.45946951791884 39.0 35.0 40.5 29.5 41.0 38-39 36.015350023011585 38.0 35.0 40.0 28.5 41.0 40-41 36.13819842160724 38.0 35.0 40.0 29.0 41.0 42-43 36.02018750586454 38.0 35.0 40.0 28.0 41.0 44-45 35.87920568982024 38.0 35.0 40.0 27.5 41.0 46-47 35.8441987022224 38.0 35.0 40.0 27.5 41.0 48-49 35.917920105271186 38.5 35.0 40.0 27.5 41.0 50-51 34.936634952757316 38.0 34.0 39.5 25.5 40.5 52-53 34.909933776588275 38.0 33.5 39.5 25.0 40.5 54-55 35.047598606188046 38.5 34.0 40.0 23.5 41.0 56-57 34.699365411980075 39.0 34.0 41.0 21.0 41.0 58-59 34.50719920160729 38.0 33.0 40.5 20.0 41.0 60-61 34.35706269251075 38.0 33.0 40.0 20.0 41.0 62-63 34.13117874567621 38.0 33.0 40.0 20.0 41.0 64-65 33.8864273110416 37.0 33.0 40.0 19.5 41.0 66-67 33.62336320590043 37.0 32.5 40.0 19.0 41.0 68-69 33.34929936564046 36.0 32.0 39.5 19.0 41.0 70-71 33.11520216779883 36.0 32.0 39.0 19.0 41.0 72-73 32.814059354260074 35.0 32.0 39.0 19.0 41.0 74-75 32.4492359312443 35.0 31.0 38.5 18.5 40.0 76-77 32.058587449554565 35.0 31.0 37.0 18.0 39.5 78-79 31.6951428392947 35.0 31.0 37.0 17.0 39.0 80-81 31.331750649649535 35.0 30.5 36.0 15.5 39.0 82-83 31.009430725625414 35.0 30.0 36.0 15.0 37.5 84-85 30.70305929978833 35.0 30.0 35.5 13.0 37.0 86-87 30.426033837597664 34.0 30.0 35.0 11.5 37.0 88-89 30.193637297950204 34.0 29.0 35.0 11.0 36.0 90-91 29.98031673423361 34.0 29.0 35.0 10.0 36.0 92-93 29.8284602889308 34.0 29.0 35.0 10.0 36.0 94-95 29.692918974554114 34.0 29.0 35.0 10.0 35.0 96-97 29.51917428730163 34.0 29.0 35.0 10.0 35.0 98-99 29.38523075014549 34.0 29.0 35.0 10.0 35.0 100 29.282244039537243 34.0 28.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5555493286704767 1101 2 0.47382051050476903 1101 3 0.5307378789969128 1101 4 0.4610390107207252 1101 5 0.5484637829267882 1101 6 0.5395677277672632 1101 7 0.5494271829616224 1101 8 0.6430506935310589 1101 9 0.6538437471088585 1101 10-11 0.6398856888600122 1101 12-13 0.5998231851967333 1101 14-15 0.638557579787026 1101 16-17 0.645935843107388 1101 18-19 0.7251121701777663 1101 20-21 0.7002174024749053 1101 22-23 0.675552155389525 1101 24-25 0.6366854823920463 1101 26-27 0.6074075683662485 1101 28-29 0.629062085085458 1101 30-31 0.5153431733285103 1101 32-33 0.6037587061135881 1101 34-35 0.5050731286697214 1101 36-37 0.5818023469588098 1101 38-39 0.6040537760279108 1101 40-41 0.5876244625982494 1101 42-43 0.5559424707375626 1101 44-45 0.5200825969615508 1101 46-47 0.537377752402243 1101 48-49 0.5758830554196734 1101 50-51 0.5297747371706549 1101 52-53 0.5284453596870122 1101 54-55 0.6019213194034378 1101 56-57 0.6013309573441035 1101 58-59 0.5258331735040755 1101 60-61 0.471587493717081 1101 62-63 0.4867254082592254 1101 64-65 0.40040906968646794 1101 66-67 0.4176783146042382 1101 68-69 0.3059012650971269 1101 70-71 0.3300572391235974 1101 72-73 0.2998443912155082 1101 74-75 0.28864780951554536 1101 76-77 0.2985012627065444 1101 78-79 0.17137292003479487 1101 80-81 0.20670496819968065 1101 82-83 0.16090314785986592 1101 84-85 0.13774447484927776 1101 86-87 0.18749808485315 1101 88-89 0.15356525761520246 1101 90-91 0.13205243527465882 1101 92-93 0.07749861572567696 1101 94-95 0.005706542464061215 1101 96-97 -0.05008930369713127 1101 98-99 0.015075186278323827 1101 100 0.05711740524410658 1102 1 0.48408888687335505 1102 2 0.47249439582716235 1102 3 0.5002648723258467 1102 4 0.43689077221993244 1102 5 0.37323090941819714 1102 6 0.4350154864741711 1102 7 0.5134054628120168 1102 8 0.6088241409347788 1102 9 0.616966595641891 1102 10-11 0.5675292692111498 1102 12-13 0.5133760207420792 1102 14-15 0.6110432332765114 1102 16-17 0.6627918739736955 1102 18-19 0.5701608377121161 1102 20-21 0.5473623853251723 1102 22-23 0.5711884124337558 1102 24-25 0.5377998113200775 1102 26-27 0.5225636149995481 1102 28-29 0.5122325313776201 1102 30-31 0.5095343054700194 1102 32-33 0.4911397661221102 1102 34-35 0.45639092064482156 1102 36-37 0.5236304317979403 1102 38-39 0.5035808146283713 1102 40-41 0.4915924785008201 1102 42-43 0.5003559070957948 1102 44-45 0.4711847304111245 1102 46-47 0.46978039780184844 1102 48-49 0.5082244832886502 1102 50-51 0.4776829397295117 1102 52-53 0.4692013545617755 1102 54-55 0.5176779996956853 1102 56-57 0.5474235874157074 1102 58-59 0.47596406689140025 1102 60-61 0.41852588135268576 1102 62-63 0.45350816310443065 1102 64-65 0.35251809009253776 1102 66-67 0.2623002434061945 1102 68-69 0.3680843795772759 1102 70-71 0.3995498791669405 1102 72-73 0.3271360251377402 1102 74-75 0.35346406440218203 1102 76-77 0.294385957291567 1102 78-79 0.2140832124311025 1102 80-81 0.26611451730543934 1102 82-83 0.23072672236674663 1102 84-85 0.13741228784616766 1102 86-87 0.15909693486573318 1102 88-89 0.17314732625537488 1102 90-91 0.1707042422538798 1102 92-93 0.06499659866623375 1102 94-95 0.0937970456634325 1102 96-97 0.1201334790497448 1102 98-99 0.08152692970658038 1102 100 0.10078371689084875 1103 1 0.4772332015483549 1103 2 0.4710473118697358 1103 3 0.3905710962221036 1103 4 0.37318367015430454 1103 5 0.46145309856746763 1103 6 0.43708706348471793 1103 7 0.41501591985478825 1103 8 0.534823194392466 1103 9 0.5736460848063487 1103 10-11 0.5553379400371838 1103 12-13 0.602972548263395 1103 14-15 0.5921420583586325 1103 16-17 0.6741524957915885 1103 18-19 0.6452379568642641 1103 20-21 0.6611609837513441 1103 22-23 0.6108121025762259 1103 24-25 0.5584334569749956 1103 26-27 0.5234156173792357 1103 28-29 0.4914861018185306 1103 30-31 0.4909796085585114 1103 32-33 0.5220528310158699 1103 34-35 0.5289299997394181 1103 36-37 0.5341372068265997 1103 38-39 0.5328374324458878 1103 40-41 0.48702946902517397 1103 42-43 0.48236052819531494 1103 44-45 0.4562970359104952 1103 46-47 0.46780284630907687 1103 48-49 0.4465834368154802 1103 50-51 0.4069970427595777 1103 52-53 0.43116907096214874 1103 54-55 0.4912816378533975 1103 56-57 0.5138602470932767 1103 58-59 0.47007894450680965 1103 60-61 0.3873157307031505 1103 62-63 0.4352452877374944 1103 64-65 0.3625590765861091 1103 66-67 0.3304203541265096 1103 68-69 0.31294100613772713 1103 70-71 0.38095968508157085 1103 72-73 0.3297361360390525 1103 74-75 0.3366232930868378 1103 76-77 0.2942754790633515 1103 78-79 0.2423790385450566 1103 80-81 0.2082984343545604 1103 82-83 0.17771082381640468 1103 84-85 0.14709863592022643 1103 86-87 0.1582873847438684 1103 88-89 0.11349657023302129 1103 90-91 0.1668948482775896 1103 92-93 0.10661225241651806 1103 94-95 0.10996077629782874 1103 96-97 0.19126749273621257 1103 98-99 0.15688477835664472 1103 100 0.09291524798502948 1104 1 0.43993425956906407 1104 2 0.48637110994652133 1104 3 0.4096626012129718 1104 4 0.38946224623536096 1104 5 0.4600410358117273 1104 6 0.44617024510075254 1104 7 0.5144475251341092 1104 8 0.5438336510724469 1104 9 0.6124133948130748 1104 10-11 0.5603116686498808 1104 12-13 0.533228120894158 1104 14-15 0.5570831181331641 1104 16-17 0.6162201492535999 1104 18-19 0.5974060830445964 1104 20-21 0.5189478729755237 1104 22-23 0.5913692993204123 1104 24-25 0.5589873472594107 1104 26-27 0.4474169426295518 1104 28-29 0.5054130191487118 1104 30-31 0.5102409305666598 1104 32-33 0.45719541913901907 1104 34-35 0.5326219359819646 1104 36-37 0.5342958562522213 1104 38-39 0.48413881212099596 1104 40-41 0.47959230168734734 1104 42-43 0.42710805332885826 1104 44-45 0.4297062351817118 1104 46-47 0.45818108223291176 1104 48-49 0.4815845045981888 1104 50-51 0.4107069387194926 1104 52-53 0.44204904227220965 1104 54-55 0.5137546991223942 1104 56-57 0.45989442818783743 1104 58-59 0.4150093052139141 1104 60-61 0.3615661609165244 1104 62-63 0.4023016027641262 1104 64-65 0.38208646324928 1104 66-67 0.3141905981045099 1104 68-69 0.3714570475897858 1104 70-71 0.40489511592826233 1104 72-73 0.3749039051263665 1104 74-75 0.3873009505960141 1104 76-77 0.3672102508127608 1104 78-79 0.30345922948660586 1104 80-81 0.29972934753582336 1104 82-83 0.2481921911500926 1104 84-85 0.25028511181587376 1104 86-87 0.23952789166030541 1104 88-89 0.2363070402167402 1104 90-91 0.28064067561682293 1104 92-93 0.25009058012700436 1104 94-95 0.1948489501850439 1104 96-97 0.2250079769870048 1104 98-99 0.15627862339008658 1104 100 0.20183850903258715 1105 1 0.4586832117765489 1105 2 0.4280065352393123 1105 3 0.4085047972416902 1105 4 0.35154126511291395 1105 5 0.4389951465859454 1105 6 0.4028453438288153 1105 7 0.42469661643352197 1105 8 0.4926613803924411 1105 9 0.5250323165233652 1105 10-11 0.48289292663864813 1105 12-13 0.4852581543319019 1105 14-15 0.5486119088241352 1105 16-17 0.5302230663311178 1105 18-19 0.5304549513588555 1105 20-21 0.5803296704379477 1105 22-23 0.5689649620208925 1105 24-25 0.5226922504886673 1105 26-27 0.5115778396233495 1105 28-29 0.5041049841462453 1105 30-31 0.49572150937239456 1105 32-33 0.46645524090715185 1105 34-35 0.512268036712527 1105 36-37 0.530804207353853 1105 38-39 0.4260583688568218 1105 40-41 0.3965240281148823 1105 42-43 0.436765325420005 1105 44-45 0.4199450663394444 1105 46-47 0.3704007133156537 1105 48-49 0.44044195116051554 1105 50-51 0.313551343394856 1105 52-53 0.4108015774826086 1105 54-55 0.4629358332750684 1105 56-57 0.4798452440993799 1105 58-59 0.3838969629583673 1105 60-61 0.30636101483767675 1105 62-63 0.3265102399542954 1105 64-65 0.32949744384208657 1105 66-67 0.3803773821993488 1105 68-69 0.4069177803490831 1105 70-71 0.38524645208047303 1105 72-73 0.337114408656241 1105 74-75 0.3193655502315451 1105 76-77 0.2887263685419228 1105 78-79 0.2212723754183834 1105 80-81 0.28417119761856213 1105 82-83 0.27711265253332584 1105 84-85 0.19284724210424642 1105 86-87 0.2950852937830142 1105 88-89 0.232862632871921 1105 90-91 0.2682912610346655 1105 92-93 0.2931047235706181 1105 94-95 0.2558271188345742 1105 96-97 0.26694631834416427 1105 98-99 0.3093620590172925 1105 100 0.28289683612301886 1106 1 0.4228522857861172 1106 2 0.42466248993798317 1106 3 0.3855519107851002 1106 4 0.3464120410889109 1106 5 0.41190627664783364 1106 6 0.44856219755855875 1106 7 0.4416748293269137 1106 8 0.4973089107558408 1106 9 0.5718200281226586 1106 10-11 0.48051981567724766 1106 12-13 0.43494612708064295 1106 14-15 0.48621556688178913 1106 16-17 0.5934828082972743 1106 18-19 0.5186459585731384 1106 20-21 0.5799129765280213 1106 22-23 0.5819362658645346 1106 24-25 0.498708897373497 1106 26-27 0.4581147426420884 1106 28-29 0.4983531699492545 1106 30-31 0.46673653767663126 1106 32-33 0.41415431143351356 1106 34-35 0.44711731741414695 1106 36-37 0.4959278565014884 1106 38-39 0.4637421969910136 1106 40-41 0.47163830820321095 1106 42-43 0.4234023680217618 1106 44-45 0.4443214552268202 1106 46-47 0.45111858157913076 1106 48-49 0.48787963639008325 1106 50-51 0.4720581631624299 1106 52-53 0.38411268177532065 1106 54-55 0.3936008993413296 1106 56-57 0.43349699150762433 1106 58-59 0.42845912369612194 1106 60-61 0.4301328697339457 1106 62-63 0.45487311298398225 1106 64-65 0.42561597358709236 1106 66-67 0.3876721548064239 1106 68-69 0.4458994583953171 1106 70-71 0.47804931609822887 1106 72-73 0.41854084877257947 1106 74-75 0.4015816541439108 1106 76-77 0.4032347993922798 1106 78-79 0.3376242661221518 1106 80-81 0.2824848700203262 1106 82-83 0.3213092122033352 1106 84-85 0.31915631827580526 1106 86-87 0.3272653611832297 1106 88-89 0.36581340960497855 1106 90-91 0.37325603261170315 1106 92-93 0.37672073467028966 1106 94-95 0.3197067916534486 1106 96-97 0.34147932395769587 1106 98-99 0.35243845667637075 1106 100 0.35747364903701495 1107 1 0.405506983129996 1107 2 0.2154307087868581 1107 3 0.3488016171863677 1107 4 0.3103318745766188 1107 5 0.375378807089767 1107 6 0.37841070286256695 1107 7 0.4140179459468314 1107 8 0.4613076688069029 1107 9 0.5084233186778278 1107 10-11 0.411961563258167 1107 12-13 0.4584799457724884 1107 14-15 0.42348688926063716 1107 16-17 0.5004584093991795 1107 18-19 0.4688860014129759 1107 20-21 0.4272742911537506 1107 22-23 0.47611986896041714 1107 24-25 0.4514550616879234 1107 26-27 0.3734731229131185 1107 28-29 0.4387149729810531 1107 30-31 0.4204305566069877 1107 32-33 0.3615929966626581 1107 34-35 0.37853891692044783 1107 36-37 0.4079072962820902 1107 38-39 0.4012755615980055 1107 40-41 0.41091287099779095 1107 42-43 0.42499406765819714 1107 44-45 0.4649110451609104 1107 46-47 0.3498577372555758 1107 48-49 0.44770914806020556 1107 50-51 0.34699927210034787 1107 52-53 0.3564830646745065 1107 54-55 0.4668877369392348 1107 56-57 0.45826028014720066 1107 58-59 0.41626478640569786 1107 60-61 0.40607922676959163 1107 62-63 0.4227333564323885 1107 64-65 0.42278456017360355 1107 66-67 0.40122409059890884 1107 68-69 0.4059794986519947 1107 70-71 0.44899783174213326 1107 72-73 0.3735360760925559 1107 74-75 0.3584993577573954 1107 76-77 0.3754298193337746 1107 78-79 0.35183516103866097 1107 80-81 0.32319111985479765 1107 82-83 0.2980431117278073 1107 84-85 0.2802441401596063 1107 86-87 0.30797245968764386 1107 88-89 0.34134047231519915 1107 90-91 0.3664852442433286 1107 92-93 0.31695248745018034 1107 94-95 0.33782402231629405 1107 96-97 0.3471162219442441 1107 98-99 0.4174032359303972 1107 100 0.3715414406122086 1108 1 0.2767075410889248 1108 2 0.1775367651423423 1108 3 0.25376479783158246 1108 4 0.2442283925306583 1108 5 0.27398684290911035 1108 6 0.29133460386285037 1108 7 0.3042305416064508 1108 8 0.42972115690186996 1108 9 0.3889449125061688 1108 10-11 0.3944456926225328 1108 12-13 0.3105557412208455 1108 14-15 0.3471127540487231 1108 16-17 0.4190163331195862 1108 18-19 0.35260550154713854 1108 20-21 0.38130340806085883 1108 22-23 0.3930023263253659 1108 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2316 50-51 -2.787896977853123 2316 52-53 -2.23977545273668 2316 54-55 -5.345477433640831 2316 56-57 -3.0954572240742237 2316 58-59 -4.8684207798556685 2316 60-61 -2.286778448303508 2316 62-63 -2.9616212508399506 2316 64-65 -1.988242507095876 2316 66-67 -1.5892200545264004 2316 68-69 -1.4965326422077467 2316 70-71 -1.525401908545291 2316 72-73 -1.3931348615245298 2316 74-75 -1.4326116528607606 2316 76-77 -1.4858387913381748 2316 78-79 -1.402201443562813 2316 80-81 -1.419742346957861 2316 82-83 -1.51857437620097 2316 84-85 -1.2874544568860742 2316 86-87 -1.2540841523551514 2316 88-89 -1.3622991157104174 2316 90-91 -2.1659085923917623 2316 92-93 -1.4177204808297255 2316 94-95 -1.3762031980947427 2316 96-97 -1.5605138970889953 2316 98-99 -1.576838848547279 2316 100 -1.5906154704478048 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 3.0 4 4.0 5 53.0 6 562.0 7 7068.0 8 21485.0 9 39189.0 10 51307.0 11 55009.0 12 61541.0 13 74461.0 14 94354.0 15 121034.0 16 151841.0 17 184584.0 18 211030.0 19 239463.0 20 269947.0 21 323763.0 22 411804.0 23 554971.0 24 738182.0 25 853213.0 26 812681.0 27 717930.0 28 687038.0 29 732049.0 30 836801.0 31 997028.0 32 1224835.0 33 1546538.0 34 2047715.0 35 2940726.0 36 4320380.0 37 6796498.0 38 8054780.0 39 2331503.0 40 4008.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.768156815924616 37.083618806021576 27.892010991646178 18.256213386407627 2 19.20787336791532 35.215331997121524 27.78628618383371 17.79050845112945 3 25.70298526559708 27.25611132816922 27.619252659584966 19.421650746648737 4 18.44257356635103 27.57484987263057 27.285586918325006 26.696989642693396 5 19.088173120210595 34.600220128177625 27.224695482906547 19.08691126870523 6 26.60826686279992 27.21142438103578 27.332698226616987 18.847610529547314 7 19.206408724953633 27.206737321061038 34.628543598492946 18.958310355492383 8 26.43962847975682 27.420660182657986 27.237156822402152 18.90255451518304 9 26.03231566614631 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18.83757769388686 34-35 18.933917846946606 27.853548934504012 34.39286141090398 18.819671807645395 36-37 18.886315344041947 27.842069663875364 34.41511143368699 18.856503558395698 38-39 18.91984163477393 27.797619640316512 34.359552810774794 18.922985914134767 40-41 19.003441750564058 27.822806999664994 34.28631238980222 18.88743885996873 42-43 19.02061545440657 27.909377235174382 34.28225503008031 18.787752280338733 44-45 19.145101828619822 27.94607476823692 34.110701687989014 18.798121715154252 46-47 19.038724906100025 28.00415067438203 34.10687062223013 18.85025379728781 48-49 19.178133461449246 28.02459641696059 33.94752455931465 18.849745562275512 50-51 19.187549928428467 28.1019132939902 33.84861940445405 18.861917373127284 52-53 19.291507094476923 28.329441814589064 33.57081608199401 18.80823500894001 54-55 19.73132244823373 28.559318467041557 32.67519775188792 19.034161332836796 56-57 20.225069963294086 29.228155921464545 31.349080580799548 19.197693534441818 58-59 20.513051043615814 29.67935229913021 30.591907802045288 19.215688855208693 60-61 20.667741329273976 30.04859000927921 30.099250445590492 19.184418215856326 62-63 20.862012092183758 30.18623111610698 29.838327490678353 19.11342930103091 64-65 21.081398035137553 30.267539924414965 29.647276546524694 19.003785493922788 66-67 21.266473234173095 30.101437805833207 29.75247950779083 18.879609452202867 68-69 21.442983765679113 29.849499921604927 29.936549791300582 18.770966521415378 70-71 21.57284466406029 29.641349889984447 30.15900466801144 18.62680077794382 72-73 21.783233291355142 29.501226181133866 30.14059424075535 18.57494628675564 74-75 21.951505467258 29.552172676752598 29.976935122137 18.519386733852407 76-77 22.276769709307953 29.63954024979053 29.596665784526856 18.487024256374664 78-79 22.513559027467203 29.806010507537746 29.266687689785304 18.413742775209748 80-81 22.834345496873336 29.91352796120631 28.906471262886583 18.345655279033775 82-83 23.068225291811245 30.027237115977783 28.572795117649424 18.331742474561548 84-85 23.218493050163588 30.091472890587802 28.360445150388593 18.32958890886002 86-87 23.40072082601222 30.073592982823495 28.163176693757645 18.362509497406645 88-89 23.685072844775203 30.029337116010062 27.969387388858095 18.316202650356637 90-91 23.921431679208364 29.925247504581726 27.84926527706298 18.304055539146937 92-93 24.10585034582436 29.802681780745598 27.846939530059934 18.24452834337011 94-95 24.2782767321688 29.788077871422413 27.752146785131295 18.181498611277497 96-97 24.41459257996045 29.821414879645896 27.590807774840677 18.173184765552982 98-99 24.511681015559347 29.83611822435813 27.45204011599196 18.20016064409056 100 24.568898704266505 29.84526272806267 27.332752134115797 18.25308643355503 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 178931.0 1 104102.5 2 23307.5 3 16662.5 4 15952.0 5 16053.5 6 16651.0 7 17186.0 8 17162.0 9 17368.5 10 19112.5 11 26880.5 12 220024.0 13 257364.0 14 101449.5 15 99460.0 16 111664.0 17 126689.5 18 142521.5 19 161045.5 20 184673.5 21 219046.0 22 264720.0 23 317118.5 24 371877.0 25 430678.0 26 485896.0 27 547133.0 28 650332.0 29 763928.5 30 843150.5 31 926929.0 32 1007200.0 33 1029329.0 34 1049126.5 35 1121432.5 36 1195352.5 37 1239791.5 38 1289251.5 39 1315448.5 40 1313031.5 41 1350393.5 42 1428704.5 43 1420661.0 44 1349631.5 45 1324755.0 46 1304624.0 47 1275202.0 48 1211031.0 49 1132512.5 50 1086913.0 51 1043921.0 52 989083.5 53 913639.0 54 811454.5 55 699096.5 56 587282.5 57 492170.0 58 416017.5 59 338667.5 60 270221.5 61 229421.0 62 185991.0 63 128025.0 64 82144.0 65 55130.0 66 39130.0 67 30825.0 68 24970.5 69 19798.0 70 15775.5 71 13056.0 72 11964.0 73 11530.5 74 12747.0 75 15089.5 76 11958.5 77 5344.5 78 2189.5 79 1598.5 80 1286.0 81 1014.5 82 797.5 83 656.5 84 574.5 85 488.0 86 402.5 87 327.0 88 275.5 89 247.0 90 213.0 91 181.5 92 167.0 93 127.5 94 101.0 95 92.5 96 78.0 97 45.5 98 27.5 99 22.5 100 14.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 8.152586932670173E-4 2 0.0013760735905462393 3 9.087278428135542E-4 4 6.568804120909407E-4 5 0.0014254045305846894 6 1.8174556856271088E-4 7 3.634911371254217E-5 8 2.8560017916997422E-5 9 1.8174556856271085E-5 10-11 0.004979828578618278 12-13 3.375274844736059E-5 14-15 7.789095795544751E-6 16-17 1.298182632590792E-5 18-19 2.726183528440663E-5 20-21 0.0012592371536130683 22-23 0.016024766416700736 24-25 0.026486820252749926 26-27 0.036416619209436896 28-29 0.046151690771235244 30-31 0.09916427675571282 32-33 0.012439185985484968 34-35 0.00635979671706229 36-37 0.001072298854519994 38-39 0.002576892525692722 40-41 0.0021407031611422158 42-43 0.0012722189799389762 44-45 9.100260254461451E-4 46-47 5.062912267104089E-4 48-49 6.750549689472118E-4 50-51 0.18428091924416068 52-53 0.29634004408939785 54-55 0.1585639212925371 56-57 0.18371491161635112 58-59 0.18201559055028974 60-61 0.1796866509074219 62-63 0.1801617857509501 64-65 0.17300750126274225 66-67 0.1820065032718616 68-69 0.16282066214480231 70-71 0.17089016538898666 72-73 0.16428111760646694 74-75 0.1573228586957803 76-77 0.1499647595342557 78-79 0.14574436779570302 80-81 0.14843420221043116 82-83 0.14421900320240885 84-85 0.1337829130190115 86-87 0.13395297494388086 88-89 0.1367544530650118 90-91 0.14695167764401246 92-93 0.14232755110672407 94-95 0.1419549726911705 96-97 0.13977532405105056 98-99 0.13686999131931238 100 0.13599761259021134 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.8515382E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.80941715381418 #Duplication Level Percentage of deduplicated Percentage of total 1 68.86512292664618 17.773686849625044 2 14.034279977216602 7.244331727708101 3 5.656660708824564 4.379853477949301 4 2.796556900282216 2.8871001453504497 5 1.6287790578276773 2.101891907743548 6 1.0807547141518148 1.6736189555097234 7 0.7430318458771009 1.3424053208167464 8 0.6004724638824608 1.239827544777684 9 0.45984804043852784 1.0681568912737778 >10 3.4945026992364543 17.813740095546233 >50 0.3324801201336427 5.886150753210296 >100 0.2318711122525369 14.224153167764845 >500 0.06574585813494163 11.06292213293357 >1k 0.009662208037643696 3.7793842277703527 >5k 1.408321222871597E-4 0.2592673303580389 >10k+ 9.053493549298859E-5 7.263509471662484 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2391102 6.208174178306216 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 196138 0.5092458903821855 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 75022 0.19478451492445278 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 42643 0.11071680400313828 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 40506 0.10516837143144522 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.13464490628705175 0.0 0.0 0.0 5.7120035833994845E-5 2 0.13470462268815092 0.0 0.0 0.0 5.7120035833994845E-5 3 0.13471760451447684 2.596365265181584E-6 0.0 0.0 5.7120035833994845E-5 4 0.134720200879742 2.596365265181584E-6 0.0 0.0 5.7120035833994845E-5 5 0.134720200879742 2.596365265181584E-6 0.0 0.0 5.7120035833994845E-5 6 0.1348084772987582 2.596365265181584E-6 0.0 0.0 5.7120035833994845E-5 7 0.1348084772987582 2.596365265181584E-6 0.0 0.0 5.7120035833994845E-5 8 0.1348084772987582 2.596365265181584E-6 0.0 0.0 5.7120035833994845E-5 9 0.1348084772987582 2.596365265181584E-6 0.0 0.0 5.971640109917643E-5 10-11 0.13481496821192113 5.192730530363168E-6 0.0 0.0 5.971640109917643E-5 12-13 0.1348617027866944 7.789095795544751E-6 0.0 0.0 5.971640109917643E-5 14-15 0.134920121005161 7.789095795544751E-6 0.0 0.0 5.971640109917643E-5 16-17 0.13494219010991504 7.789095795544751E-6 0.0 0.0 6.1014583731767224E-5 18-19 0.13498243377152536 7.789095795544751E-6 0.0 0.0 6.231276636435801E-5 20-21 0.13500709924154458 1.298182632590792E-5 0.0 0.0 6.231276636435801E-5 22-23 0.1350590265468482 1.298182632590792E-5 0.0 0.0 6.361094899694881E-5 24-25 0.13529789215124494 1.5578191591089502E-5 0.0 0.0 6.49091316295396E-5 26-27 0.13627802003885098 1.5578191591089502E-5 0.0 0.0 6.620731426213038E-5 28-29 0.13997134962857177 1.5578191591089502E-5 0.0 0.0 6.750549689472118E-5 30-31 0.14984402854942475 2.3367287386634254E-5 0.0 5.192730530363168E-6 6.750549689472118E-5 32-33 0.17238437359909867 2.4665470019225047E-5 0.0 7.789095795544751E-6 7.010186215990277E-5 34-35 0.2150673203760513 2.8560017916997422E-5 0.0 1.0385461060726336E-5 7.140004479249355E-5 36-37 0.2838398435201811 2.8560017916997422E-5 0.0 1.1683643693317127E-5 7.269822742508434E-5 38-39 0.3851915580118094 2.8560017916997422E-5 0.0 1.298182632590792E-5 7.269822742508434E-5 40-41 0.5235622484543967 2.8560017916997422E-5 0.0 1.298182632590792E-5 7.269822742508434E-5 42-43 0.7041329617346129 3.1156383182179005E-5 0.0 1.298182632590792E-5 7.269822742508434E-5 44-45 0.9305152419363256 3.1156383182179005E-5 0.0 1.298182632590792E-5 7.789095795544751E-5 46-47 1.2058909346920146 3.1156383182179005E-5 0.0 1.298182632590792E-5 7.789095795544751E-5 48-49 1.5327356742820308 3.1156383182179005E-5 0.0 1.298182632590792E-5 8.04873232206291E-5 50-51 1.9076521167568843 3.375274844736059E-5 0.0 1.4280008958498711E-5 8.308368848581068E-5 52-53 2.3261926884173185 4.413820950808693E-5 0.0 1.6876374223680295E-5 8.568005375099227E-5 54-55 2.793708498074873 4.673457477326851E-5 0.0 1.9472739488861878E-5 8.568005375099227E-5 56-57 3.3110083135096517 4.933094003845009E-5 0.0 2.596365265181584E-5 8.568005375099227E-5 58-59 3.8709053956676325 5.0629122671040886E-5 0.0 2.596365265181584E-5 8.568005375099227E-5 60-61 4.470066011548321 5.192730530363168E-5 0.0 2.596365265181584E-5 8.568005375099227E-5 62-63 5.102103102599372 5.192730530363168E-5 0.0 2.596365265181584E-5 8.697823638358307E-5 64-65 5.765823638981433 5.841821846658564E-5 0.0 2.596365265181584E-5 8.827641901617386E-5 66-67 6.459803514346554 5.971640109917643E-5 0.0 2.596365265181584E-5 8.827641901617386E-5 68-69 7.182409614942934 5.971640109917643E-5 0.0 2.596365265181584E-5 8.827641901617386E-5 70-71 7.931129957376509 5.971640109917643E-5 0.0 2.596365265181584E-5 9.087278428135543E-5 72-73 8.700136999809583 6.750549689472118E-5 0.0 2.596365265181584E-5 9.60655148117186E-5 74-75 9.487802821220882 7.010186215990277E-5 0.0 2.596365265181584E-5 9.60655148117186E-5 76-77 10.293967745146603 7.269822742508434E-5 0.0 2.596365265181584E-5 9.736369744430939E-5 78-79 11.118303331380694 7.659277532285673E-5 0.0 2.596365265181584E-5 1.0125824534208177E-4 80-81 11.95843001115762 7.91891405880383E-5 0.0 2.8560017916997422E-5 1.0125824534208177E-4 82-83 12.80810871874515 8.697823638358307E-5 0.0 2.8560017916997422E-5 1.0645097587244493E-4 84-85 13.67117688200522 9.346914954653701E-5 0.0 2.8560017916997422E-5 1.0774915850503573E-4 86-87 14.544572088107552 1.0255642797467256E-4 0.0 2.8560017916997422E-5 1.0904734113762652E-4 88 15.207833067837676 1.1424007166798969E-4 0.0 2.8560017916997422E-5 1.0904734113762652E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 298610 0.0 87.704254 8 GTGGTAT 299455 0.0 87.26554 6 AGTGGTA 301250 0.0 86.94892 5 TGGTATC 299920 0.0 86.77966 7 GCAGTGG 308255 0.0 86.60917 3 GTATCAA 302495 0.0 86.127846 9 AAGCAGT 320200 0.0 83.71815 1 CAGTGGT 322765 0.0 81.64459 4 AGCAGTG 333170 0.0 80.64774 2 CAACGCA 297130 0.0 45.67746 12-13 ATCAACG 297205 0.0 45.656315 10-11 ACGCAGA 302135 0.0 45.0815 14-15 AGAGTAC 300525 0.0 44.492966 18-19 TCAACGC 297715 0.0 44.083725 12-13 AACGCAG 299150 0.0 43.654068 14-15 TATCAAC 299545 0.0 43.398945 10-11 CGCAGAG 302435 0.0 42.928642 16-17 AGTACTT 313660 0.0 42.67033 20-21 GAGTACT 298960 0.0 42.397793 20-21 GCAGAGT 326450 0.0 41.095005 16-17 >>END_MODULE