FastQCFastQC Report
Thu 26 May 2016
SRR1660685_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660685_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38515382
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19427955.044205455368455No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2281370.592326982502731No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT782670.20320972020996703No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730860.18975795177106125No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT402650.10454264740253646No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2487800.087.209598
TGGTATC2515150.086.225517
GTGGTAT2545950.085.963796
AGTGGTA2561900.085.714565
GTATCAA2524850.085.309469
GCAGTGG2588050.085.227743
AAGCAGT2597850.081.4748841
CAGTGGT2771250.079.408854
AGCAGTG2807700.078.366242
CAACGCA2441950.045.79655512-13
ATCAACG2442550.045.7019910-11
ACGCAGA2530950.044.73126214-15
TCAACGC2441350.044.0758312-13
AGAGTAC2559450.043.99205818-19
AACGCAG2490150.043.26894414-15
TATCAAC2494850.043.1259110-11
CGCAGAG2533950.042.5275116-17
GAGTACT2533000.041.95960620-21
AGTACTT2685400.041.8404420-21
CAGAGTA2678100.039.98608818-19
Produced by FastQC (version 0.11.5)