##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660685_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38515382 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.573470256636686 31.0 30.0 34.0 16.0 34.0 2 30.59695689893456 31.0 30.0 34.0 25.0 34.0 3 31.374847353195147 31.0 31.0 34.0 28.0 34.0 4 35.11981472752886 37.0 35.0 37.0 32.0 37.0 5 35.39125799141756 37.0 35.0 37.0 33.0 37.0 6 35.53841620991842 37.0 35.0 37.0 33.0 37.0 7 35.566542011708464 37.0 35.0 37.0 33.0 37.0 8 35.59946690909102 37.0 35.0 37.0 33.0 37.0 9 37.24958651584969 39.0 37.0 39.0 33.0 39.0 10-11 37.19472991336292 39.0 37.0 39.0 33.0 39.0 12-13 37.01878909055088 39.0 37.0 39.0 33.5 39.0 14-15 38.3393140563944 40.0 38.0 41.0 33.0 41.0 16-17 38.45248354280895 40.0 38.0 41.0 34.0 41.0 18-19 38.44004144889436 40.0 38.0 41.0 34.0 41.0 20-21 38.38139317948345 40.0 38.0 41.0 34.0 41.0 22-23 38.19565981456448 40.0 37.5 41.0 33.5 41.0 24-25 38.21512949034233 40.0 37.5 41.0 33.5 41.0 26-27 38.14017879921326 40.0 37.5 41.0 33.0 41.0 28-29 38.05487051900459 40.0 37.0 41.0 33.0 41.0 30-31 37.94290835542018 40.0 37.0 41.0 33.0 41.0 32-33 37.81337300510222 40.0 37.0 41.0 33.0 41.0 34-35 37.63482630134631 40.0 37.0 41.0 32.0 41.0 36-37 37.442051502955366 39.5 37.0 41.0 32.0 41.0 38-39 37.03228904752912 39.0 35.0 41.0 31.0 41.0 40-41 36.8883228134671 39.0 35.0 40.0 31.0 41.0 42-43 36.731633325095935 39.0 35.0 40.0 31.0 41.0 44-45 36.867418606934756 39.0 35.0 40.5 31.0 41.0 46-47 36.79507236874868 39.0 35.0 41.0 31.0 41.0 48-49 36.68708304645661 39.0 35.0 40.5 30.5 41.0 50-51 36.502192474684534 39.0 35.0 40.0 30.0 41.0 52-53 36.34738442942095 39.0 35.0 40.0 30.0 41.0 54-55 36.048630168071554 39.0 35.0 40.5 28.5 41.0 56-57 35.60138555811286 39.0 35.0 40.5 26.5 41.0 58-59 35.37802660246236 38.5 35.0 40.0 26.0 41.0 60-61 35.19790813706586 38.0 34.0 40.0 26.0 41.0 62-63 34.89512819839097 38.0 34.0 40.0 25.5 41.0 64-65 34.63351113848488 37.5 34.0 40.0 25.0 41.0 66-67 34.443316750694564 37.0 34.0 40.0 25.0 41.0 68-69 34.20478949163739 36.5 33.5 39.5 25.0 41.0 70-71 33.923474418610205 36.0 33.0 39.0 24.0 41.0 72-73 33.58933300986084 35.5 33.0 39.0 24.0 40.5 74-75 33.220886346135686 35.0 33.0 38.5 23.0 40.0 76-77 31.423870442204105 34.0 30.0 36.0 20.5 39.0 78-79 32.45108719160568 35.0 32.0 37.0 22.0 39.0 80-81 32.3884192684367 35.0 32.0 36.5 22.0 39.0 82-83 32.12091077014373 35.0 32.0 36.0 20.5 38.0 84-85 31.85495816969958 35.0 32.0 36.0 20.0 37.0 86-87 31.595558392748124 35.0 32.0 35.0 20.0 37.0 88-89 31.359688513539865 35.0 32.0 35.0 19.5 36.0 90-91 31.151105602431777 35.0 31.5 35.0 19.0 36.0 92-93 30.93655991780115 34.5 31.0 35.0 18.5 36.0 94-95 30.746269230823156 34.0 31.0 35.0 18.0 35.5 96-97 30.589127273877224 34.0 31.0 35.0 17.0 35.0 98-99 30.44079962649728 34.0 31.0 35.0 15.5 35.0 100 30.334397695964693 34.0 31.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.8198091238020844 1101 2 2.03186677751118 1101 3 1.3230811129178157 1101 4 1.0934403732925801 1101 5 0.6555678858743264 1101 6 0.4472553620192983 1101 7 0.37230100421406576 1101 8 0.3653632592041731 1101 9 0.3976384779935387 1101 10-11 0.3463563996012837 1101 12-13 0.5940234365861627 1101 14-15 0.6400979221123748 1101 16-17 0.44368860263485743 1101 18-19 0.41244948236853674 1101 20-21 0.3968694834958555 1101 22-23 0.40878467259022244 1101 24-25 0.4132683027304296 1101 26-27 0.4221728998402128 1101 28-29 0.3624887532849641 1101 30-31 0.3540593464354842 1101 32-33 0.36890514126905316 1101 34-35 0.3382673215677059 1101 36-37 0.3311083754166475 1101 38-39 0.3895962659305994 1101 40-41 0.3766829320263767 1101 42-43 0.38048405168567 1101 44-45 0.2535254371216311 1101 46-47 0.20767762292512515 1101 48-49 0.19441300697216235 1101 50-51 0.24707000138719337 1101 52-53 0.24094407140227503 1101 54-55 0.26468362992503813 1101 56-57 0.2174544613137286 1101 58-59 0.2105353777010066 1101 60-61 0.24098891473075668 1101 62-63 0.27185019571022195 1101 64-65 0.31238678286891997 1101 66-67 0.22467036890252956 1101 68-69 0.18769065327251866 1101 70-71 0.1378487082369304 1101 72-73 0.13053837983837724 1101 74-75 0.09019860383764211 1101 76-77 0.13252469868414707 1101 78-79 0.06614997882663687 1101 80-81 0.08843559199352313 1101 82-83 0.15631376340497383 1101 84-85 0.04056038386949723 1101 86-87 0.05331872406251392 1101 88-89 -0.07128125293120391 1101 90-91 -0.12233615411407683 1101 92-93 -0.06352736141800364 1101 94-95 -0.15632576654462582 1101 96-97 -0.1708520588774256 1101 98-99 -0.22625025246540886 1101 100 -0.2669174367723599 1102 1 2.7771554603845665 1102 2 2.0240228289226856 1102 3 1.2987666468609866 1102 4 1.079984062294571 1102 5 0.6522999487237513 1102 6 0.4585947828969026 1102 7 0.33196327964889605 1102 8 0.30899841345143386 1102 9 0.312658499238303 1102 10-11 0.284200384592566 1102 12-13 0.5538802267779843 1102 14-15 0.6080815404065092 1102 16-17 0.30675332596371874 1102 18-19 0.3597320748379502 1102 20-21 0.3677718505546963 1102 22-23 0.35496722081332877 1102 24-25 0.3126357823883268 1102 26-27 0.3188689363495527 1102 28-29 0.277825073518585 1102 30-31 0.25660731264999725 1102 32-33 0.29835454940412376 1102 34-35 0.27840355904202596 1102 36-37 0.3078779707645225 1102 38-39 0.3429224396221997 1102 40-41 0.33202754322604733 1102 42-43 0.30466497341928545 1102 44-45 0.3008174906309904 1102 46-47 0.22836317483773172 1102 48-49 0.20722648955483436 1102 50-51 0.22856846780256745 1102 52-53 0.1744047000051765 1102 54-55 0.28552993328521126 1102 56-57 0.25311308552201695 1102 58-59 0.24844556254700478 1102 60-61 0.207073250507122 1102 62-63 0.2568667237885691 1102 64-65 0.19015686248570773 1102 66-67 0.13446772581862376 1102 68-69 0.13049685690837265 1102 70-71 0.14838115184037548 1102 72-73 0.11844961829118716 1102 74-75 0.12234653358060399 1102 76-77 0.13586704323471466 1102 78-79 0.2377458635404608 1102 80-81 0.26783974224438367 1102 82-83 0.21775646190111786 1102 84-85 0.12745713294177818 1102 86-87 0.08127685025643316 1102 88-89 0.038203621557794776 1102 90-91 -0.059868912356005666 1102 92-93 0.02353504624626268 1102 94-95 -0.1147018680327676 1102 96-97 -0.15240984218331732 1102 98-99 -0.2553643662622669 1102 100 -0.38726446817047133 1103 1 2.784898035709073 1103 2 1.9916350972582855 1103 3 1.2761525508867884 1103 4 1.0665358221554726 1103 5 0.614987483508358 1103 6 0.4009521466175201 1103 7 0.29421680013393114 1103 8 0.2661274648088465 1103 9 0.25974681868445515 1103 10-11 0.2617417150609711 1103 12-13 0.5351545496252399 1103 14-15 0.5754176446663024 1103 16-17 0.3381135482973363 1103 18-19 0.24829054912117243 1103 20-21 0.25807754822667306 1103 22-23 0.26379983574160093 1103 24-25 0.3168227311296903 1103 26-27 0.3028087623225417 1103 28-29 0.3063682598186048 1103 30-31 0.28279843193266174 1103 32-33 0.26532522764669864 1103 34-35 0.3093371224054522 1103 36-37 0.2606231447708609 1103 38-39 0.260585983723864 1103 40-41 0.2532099103622656 1103 42-43 0.21291849493314174 1103 44-45 0.21958270756735487 1103 46-47 0.1487884961746886 1103 48-49 0.07390199022797361 1103 50-51 0.14402572213467124 1103 52-53 0.1067571813355741 1103 54-55 0.19126218567986086 1103 56-57 0.20634472697160078 1103 58-59 0.1595875533998452 1103 60-61 0.1844417668229923 1103 62-63 0.2054089963217791 1103 64-65 0.1683616016704761 1103 66-67 0.10121793249222577 1103 68-69 0.13754070087588133 1103 70-71 0.07352398787502068 1103 72-73 0.061603366028485596 1103 74-75 0.01752196255601035 1103 76-77 -0.09900865555700733 1103 78-79 0.15447423295635332 1103 80-81 0.15204662126441093 1103 82-83 0.1384556088309381 1103 84-85 0.10430316760221814 1103 86-87 0.08157018026686202 1103 88-89 0.03500573103108806 1103 90-91 0.002346924565468811 1103 92-93 -0.014400752622872659 1103 94-95 -0.03566398275077631 1103 96-97 -0.05671625446523976 1103 98-99 -0.21490019949528616 1103 100 -0.23576694856863156 1104 1 2.7204363316890685 1104 2 1.9688227522820974 1104 3 1.2628089689988364 1104 4 1.0396836267522573 1104 5 0.6029312440464949 1104 6 0.41416446578842425 1104 7 0.33558073475714423 1104 8 0.2969542305539221 1104 9 0.3333060094075577 1104 10-11 0.24931787402515937 1104 12-13 0.5229435490247667 1104 14-15 0.5361489200105112 1104 16-17 0.31752142592557675 1104 18-19 0.3017843121360073 1104 20-21 0.31661918817260215 1104 22-23 0.3311493296269461 1104 24-25 0.3067162530244403 1104 26-27 0.27695807342416146 1104 28-29 0.2589004042062939 1104 30-31 0.25680893613860434 1104 32-33 0.2722352667475434 1104 34-35 0.2857871673966912 1104 36-37 0.22460044258318845 1104 38-39 0.27775304615767027 1104 40-41 0.31258527049627816 1104 42-43 0.2910403456130126 1104 44-45 0.22099192450441052 1104 46-47 0.242131570589315 1104 48-49 0.2135805287937913 1104 50-51 0.2298475752367537 1104 52-53 0.265887379408035 1104 54-55 0.24466563360353177 1104 56-57 0.21474309456181828 1104 58-59 0.10840126941675265 1104 60-61 0.12472372124305053 1104 62-63 0.17342149129283513 1104 64-65 0.14993412503491044 1104 66-67 0.10709248617073541 1104 68-69 0.14078117045013272 1104 70-71 0.09588561918181426 1104 72-73 0.06085034285269586 1104 74-75 0.06472892864642432 1104 76-77 0.06515960050491643 1104 78-79 0.17727545732449101 1104 80-81 0.1599732987423792 1104 82-83 0.18109361190975903 1104 84-85 0.13123791161302023 1104 86-87 0.055011037024311094 1104 88-89 0.11113402186456156 1104 90-91 0.14538204179278935 1104 92-93 0.13978589544338504 1104 94-95 0.15448967238759792 1104 96-97 0.04083134852308845 1104 98-99 0.013510952603517978 1104 100 -0.037203332558050306 1105 1 2.658557365640771 1105 2 1.9359552152667199 1105 3 1.2237728615155525 1105 4 1.021471392556741 1105 5 0.5798650283302536 1105 6 0.34083371952386443 1105 7 0.2525727188094109 1105 8 0.2285687573994224 1105 9 0.2811510918683169 1105 10-11 0.2350144224003472 1105 12-13 0.43783230257979966 1105 14-15 0.49657471027171596 1105 16-17 0.30114762443632515 1105 18-19 0.2629031132634694 1105 20-21 0.24741159162267223 1105 22-23 0.2450697108798181 1105 24-25 0.22655724787702525 1105 26-27 0.27172093644052353 1105 28-29 0.23566764108196736 1105 30-31 0.19289546328528928 1105 32-33 0.2245679904312965 1105 34-35 0.2930578051239152 1105 36-37 0.30307743393933606 1105 38-39 0.2858821442795616 1105 40-41 0.23266808221207214 1105 42-43 0.23374111480193704 1105 44-45 0.2382507097432125 1105 46-47 0.20281943469987596 1105 48-49 0.22332316277249475 1105 50-51 0.1877253879725984 1105 52-53 0.21324001419584704 1105 54-55 0.16962265625041795 1105 56-57 0.18057229527511964 1105 58-59 0.13719972160992455 1105 60-61 0.1584187707491509 1105 62-63 0.2236846603248992 1105 64-65 0.18914296235554673 1105 66-67 0.1366692099055129 1105 68-69 0.15336369158538332 1105 70-71 0.12592304632132567 1105 72-73 0.10430773968685259 1105 74-75 0.10294190879405818 1105 76-77 0.09203849810770492 1105 78-79 0.19292490822504504 1105 80-81 0.20555393851427795 1105 82-83 0.16763725764008086 1105 84-85 0.1513343858247609 1105 86-87 0.17103342162190316 1105 88-89 0.19966323139042075 1105 90-91 0.19529442053975998 1105 92-93 0.19981786029360293 1105 94-95 0.19724074925377622 1105 96-97 0.19835441381910712 1105 98-99 0.1468742800824394 1105 100 0.152738233933853 1106 1 2.6317294317132216 1106 2 1.8954591729819832 1106 3 1.1751397464558373 1106 4 1.010368238635806 1106 5 0.5899521274021637 1106 6 0.4094775088441409 1106 7 0.27463205152618286 1106 8 0.2893180308580128 1106 9 0.2677295190680411 1106 10-11 0.20917377764694578 1106 12-13 0.46853775024818134 1106 14-15 0.48494484059893495 1106 16-17 0.33669622716850967 1106 18-19 0.27459019340886925 1106 20-21 0.2471205627521016 1106 22-23 0.22651804372724627 1106 24-25 0.2404161616627718 1106 26-27 0.20406674736687336 1106 28-29 0.2257862257138541 1106 30-31 0.2552672159056115 1106 32-33 0.26677009920850026 1106 34-35 0.23183903337435652 1106 36-37 0.32783100983045443 1106 38-39 0.29154992710076755 1106 40-41 0.3569023595051064 1106 42-43 0.3715733873407814 1106 44-45 0.29123649557009657 1106 46-47 0.2471406289335505 1106 48-49 0.2565310392580926 1106 50-51 0.29025153491001277 1106 52-53 0.24735363542323086 1106 54-55 0.16672018011693268 1106 56-57 0.19544656916138337 1106 58-59 0.23201256324707487 1106 60-61 0.23596116980806414 1106 62-63 0.27237382231362517 1106 64-65 0.20514732651761847 1106 66-67 0.12533260214032538 1106 68-69 0.15166123947400223 1106 70-71 0.13364394260804602 1106 72-73 0.1465802638172491 1106 74-75 0.2179025350286068 1106 76-77 0.11887035681111513 1106 78-79 0.2705140930347696 1106 80-81 0.2632571671949293 1106 82-83 0.24807775207331417 1106 84-85 0.23422135619640017 1106 86-87 0.2125406549337221 1106 88-89 0.2238352966621271 1106 90-91 0.2689695844087616 1106 92-93 0.31173205623175804 1106 94-95 0.33114527243493086 1106 96-97 0.34953936724351564 1106 98-99 0.3040495062997337 1106 100 0.29943504208710436 1107 1 0.6639947200168308 1107 2 0.998784753785035 1107 3 0.7808013501996776 1107 4 0.786004293902387 1107 5 0.4461346494887337 1107 6 0.31706784187235826 1107 7 0.20093248779738815 1107 8 0.2230003480694478 1107 9 0.2694594787642046 1107 10-11 0.19116818133041846 1107 12-13 0.4395788362206403 1107 14-15 0.3971538723018 1107 16-17 0.2717011166712666 1107 18-19 0.2304252234653248 1107 20-21 0.23551378427665526 1107 22-23 0.22303638572977036 1107 24-25 0.2302526308881454 1107 26-27 0.19617402052488586 1107 28-29 0.19713979288855654 1107 30-31 0.15939119834156656 1107 32-33 0.21592000663918753 1107 34-35 0.13633324546130154 1107 36-37 0.2086707167868127 1107 38-39 0.28153766971200156 1107 40-41 0.19060631431720054 1107 42-43 0.21482003893658685 1107 44-45 0.2214620875112132 1107 46-47 0.23840692764490257 1107 48-49 0.24408766929249026 1107 50-51 0.16030148177743797 1107 52-53 0.2348606298756053 1107 54-55 0.20971077167892105 1107 56-57 0.2076438730234571 1107 58-59 0.16545416207856078 1107 60-61 0.17322066276115322 1107 62-63 0.28443176841771134 1107 64-65 0.1990233163634727 1107 66-67 0.17123111105423305 1107 68-69 0.19163894476364618 1107 70-71 0.13899767188335943 1107 72-73 0.11075769968901028 1107 74-75 0.03329472249063059 1107 76-77 0.01021600587201732 1107 78-79 0.21267441372403795 1107 80-81 0.2427436536716172 1107 82-83 0.16198106073238705 1107 84-85 0.17815476898370264 1107 86-87 0.1582949747080029 1107 88-89 0.2317699905811459 1107 90-91 0.25016480512570993 1107 92-93 0.23436001321632105 1107 94-95 0.2503620838783327 1107 96-97 0.3022166046980388 1107 98-99 0.30713572212500395 1107 100 0.2912207117117056 1108 1 0.8994840530474555 1108 2 1.0758154455128164 1108 3 0.7703706279068463 1108 4 0.7752075357495016 1108 5 0.38594459873951337 1108 6 0.24174122062683523 1108 7 0.14155220822201642 1108 8 0.15888452274964493 1108 9 0.14224066487377485 1108 10-11 0.06497403317670347 1108 12-13 0.34741122585761985 1108 14-15 0.33285084364825934 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-0.8523515826732257 2316 100 -0.7650482887009993 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 35.0 7 609.0 8 832.0 9 403.0 10 224.0 11 243.0 12 431.0 13 1799.0 14 7329.0 15 19601.0 16 37450.0 17 56927.0 18 78184.0 19 108739.0 20 145581.0 21 190218.0 22 270287.0 23 403081.0 24 599773.0 25 739646.0 26 716631.0 27 661676.0 28 671798.0 29 755640.0 30 887114.0 31 1076686.0 32 1320000.0 33 1654684.0 34 2184966.0 35 3118875.0 36 4599294.0 37 7434627.0 38 8623882.0 39 2145456.0 40 2661.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.68149463710855 35.24746955568122 28.491657770661437 18.579378036548793 2 19.644561610953883 33.973694188088615 28.98738833255827 17.394355868399227 3 24.493843122050382 28.336502759767583 27.914170185672056 19.255483932509975 4 18.98295384900905 28.383478797730184 27.83735412094197 24.7962132323188 5 19.332912237505525 33.96803645878418 27.580484597037103 19.118566706673192 6 25.37229151719175 27.932367385061895 27.793781196302298 18.901559901444053 7 19.263822957799043 28.05284392609685 33.71830506575269 18.965028050351414 8 24.92616067004087 28.014428105633225 27.97219043549925 19.087220788826656 9 24.71252394692593 27.771782193410417 28.42798495416714 19.087708905496513 10-11 18.782108924688842 31.331462582923365 30.925356004517884 18.961072487869913 12-13 18.805127428381265 28.372600138272517 30.691568872845203 22.130703560501015 14-15 18.773091748122866 34.54438930399289 27.562687811326914 19.119831136557337 16-17 21.93138847301245 28.266414978463345 27.595997074363503 22.206199474160705 18-19 19.01759406151028 31.382877106087122 27.630843697720564 21.968685134682033 20-21 21.98065437855452 31.37495559566305 27.59629386513679 19.048096160645635 22-23 21.944153503667284 28.45479686887156 30.586910093927543 19.014139533533612 24-25 18.895008980649862 31.29233535803043 27.545003306931843 22.267652354387867 26-27 18.976361771464454 28.269660692557373 33.68428028658173 19.06969724939644 28-29 18.97057943819632 28.388725842894537 33.57515710089394 19.065537618015206 30-31 19.0677346217983 28.349744803287663 33.58394809894777 18.998572475966263 32-33 19.033256364494868 28.45556063465305 33.435268102524006 19.075914898328072 34-35 19.072362038008116 28.393082384501373 33.38825684486599 19.146298732624523 36-37 19.079996402476286 28.40308061854352 33.41430704231364 19.10261593666655 38-39 19.217510084743076 28.413810189332523 33.295466621510656 19.073213104413746 40-41 19.228568710546917 28.50090254882155 33.279069279566535 18.991459461065 42-43 19.167189618881427 28.48477787144904 33.29734461438841 19.050687895281122 44-45 19.20827997035049 28.54223387080882 33.19488145323624 19.05460470560445 46-47 19.29699834844125 28.623884951186717 33.106288359925045 18.972828340446988 48-49 19.308921935329636 28.6937266900809 32.9953192082399 19.002032166349565 50-51 19.330807228148032 28.728698606490678 32.81703896203183 19.123455203329463 52-53 19.453831332039805 28.879880853609208 32.50846651154632 19.157821302804667 54-55 19.742650696346832 29.123638629421393 31.824211343324794 19.30949933090698 56-57 20.112003512144497 29.65860831638002 30.710690331417588 19.518697840057893 58-59 20.282874041054555 30.06784328300735 30.00747065485389 19.641812021084203 60-61 20.37680971631986 30.369078142795146 29.559841152960676 19.694270987924323 62-63 20.400609021157713 30.49479683838166 29.407994754442086 19.69659938601854 64-65 20.478977306225534 30.707082245115124 29.08289445942762 19.731045989231717 66-67 20.430169775501135 30.70368207738455 29.08434960504078 19.781798542073535 68-69 20.396361904891485 30.562326196861594 29.20343875298012 19.837873145266798 70-71 20.319590579066364 30.409893511785075 29.33656987045828 19.933946038690284 72-73 20.301554569252865 30.283094111307623 29.311714234790724 20.103637084648785 74-75 20.280437814217088 30.266457363220578 29.15199518593073 20.301109636631605 76-77 20.310146400914235 30.273632181182027 28.95343888475022 20.462782533153515 78-79 20.324339317394024 30.419767444230867 28.62298981573422 20.632903422640894 80-81 20.40843916355871 30.456527765179004 28.31352633230633 20.82150673895595 82-83 20.43995043426644 30.512359171608882 27.982218563231687 21.06547183089299 84-85 20.374726568465313 30.521105157071162 27.808530297594658 21.29563797686887 86-87 20.359732631415582 30.45193238965786 27.60309265463679 21.58524232428977 88-89 20.422450525153863 30.371816603559683 27.40460550914716 21.801127362139297 90-91 20.49375492787642 30.196228589252883 27.2754307699632 22.034585712907496 92-93 20.516333097133067 29.99959612753423 27.302825041811708 22.181245733521003 94-95 20.577617295711644 29.908648938576427 27.21335913493607 22.30037463077586 96-97 20.587427671381505 29.849135011382945 27.108940371012103 22.454496946223447 98-99 20.5597357745921 29.76818197089684 27.030352127278018 22.641730127233046 100 20.46990991806858 29.672007407326245 27.026350147585195 22.831732527019984 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 202648.0 1 120026.0 2 28553.5 3 18728.5 4 17496.0 5 17374.5 6 17735.0 7 17932.0 8 17837.0 9 18067.0 10 19539.0 11 27938.0 12 187482.0 13 214683.0 14 84310.0 15 83918.5 16 95528.5 17 108690.0 18 123195.0 19 141178.5 20 165195.5 21 202033.5 22 250920.0 23 307960.5 24 367713.0 25 429326.5 26 487634.0 27 552418.0 28 659279.0 29 777853.5 30 860228.0 31 942577.5 32 1022956.0 33 1040393.5 34 1051211.0 35 1119034.5 36 1190576.0 37 1231545.5 38 1278561.5 39 1309382.0 40 1309810.5 41 1351924.0 42 1440208.0 43 1431717.0 44 1353614.0 45 1322643.0 46 1295839.5 47 1261226.5 48 1193431.5 49 1117063.5 50 1082970.0 51 1051630.5 52 1009559.0 53 944550.5 54 846497.0 55 734807.5 56 618407.0 57 515229.0 58 431264.5 59 350337.0 60 280047.0 61 235433.0 62 186552.5 63 124303.5 64 76916.5 65 50651.5 66 35347.5 67 27544.5 68 22178.5 69 17391.0 70 13794.0 71 11223.0 72 10058.5 73 9720.0 74 11199.5 75 13994.0 76 10910.5 77 4110.0 78 1037.0 79 616.0 80 430.5 81 275.5 82 167.0 83 106.5 84 81.0 85 56.5 86 39.5 87 34.5 88 26.5 89 20.0 90 17.0 91 15.0 92 8.5 93 3.0 94 2.0 95 1.5 96 1.0 97 2.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20421451356759227 2 3.8945478977723756E-5 3 2.596365265181584E-6 4 2.596365265181584E-6 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.38152938480527077 14-15 0.0 16-17 6.49091316295396E-6 18-19 0.0 20-21 0.0 22-23 6.49091316295396E-6 24-25 1.9083284699084643E-4 26-27 4.413820950808692E-5 28-29 1.5578191591089502E-5 30-31 2.427601522944781E-4 32-33 5.919712804614011E-4 34-35 1.298182632590792E-6 36-37 0.0 38-39 5.9716401099176424E-5 40-41 3.634911371254217E-5 42-43 1.8693829909307403E-4 44-45 1.9472739488861877E-4 46-47 6.542840468257592E-4 48-49 0.0027638308247857958 50-51 0.018061614967235688 52-53 0.045516879463898346 54-55 0.058684345906266755 56-57 0.05883493509164728 58-59 0.05912572800134761 60-61 0.05188576345938877 62-63 0.02507829209638892 64-65 0.010970941428024782 66-67 0.009072998419177044 68-69 0.007010186215990277 70-71 0.008394048902332061 72-73 0.010068704498374182 74-75 0.0041619735200860795 76-77 0.004914919446988739 78-79 0.019474037671494467 80-81 0.03649061561949457 82-83 0.046262036295005464 84-85 0.046955265820808945 86-87 0.041787200760465 88-89 0.024604455435493278 90-91 0.007728081211812984 92-93 0.0019433794009884153 94-95 0.006566207755644225 96-97 0.004778610270566705 98-99 0.0010073897228904546 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.8515382E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.745289712299858 #Duplication Level Percentage of deduplicated Percentage of total 1 66.10158610622364 16.357028986410388 2 15.194917212733664 7.520052571670127 3 6.155055740904165 4.56925912512084 4 3.116714193518755 3.0849598267623435 5 1.7926291953241826 2.2179564392511932 6 1.1754534781085546 1.7452162115476022 7 0.8334771925510023 1.4437244218788217 8 0.6308478539391085 1.2488410328084691 9 0.4845239884232562 1.0790717819483249 >10 3.8248880052286687 18.685397854433184 >50 0.36015721843677967 6.110862216251669 >100 0.25411703776177186 15.144221250260617 >500 0.06595560673079184 10.634361845097066 >1k 0.009463967376285659 3.560973430810725 >5k 1.1726150691574487E-4 0.1894500315910935 >10k+ 9.594123255156815E-5 6.408622974157506 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1942795 5.044205455368455 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 228137 0.592326982502731 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 78267 0.20320972020996703 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 73086 0.18975795177106125 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 40265 0.10454264740253646 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.12894848089524336 2.596365265181584E-6 0.0 0.0 4.154184424290534E-5 2 0.12902377548793362 5.192730530363168E-6 0.0 0.0 4.413820950808693E-5 3 0.12903675731425954 7.789095795544751E-6 0.0 0.0 4.413820950808693E-5 4 0.1290393536795247 7.789095795544751E-6 0.0 0.0 4.413820950808693E-5 5 0.1290393536795247 7.789095795544751E-6 0.0 0.0 4.413820950808693E-5 6 0.12919773196070078 1.0385461060726336E-5 0.0 0.0 4.413820950808693E-5 7 0.12919773196070078 1.0385461060726336E-5 0.0 0.0 4.413820950808693E-5 8 0.12919773196070078 1.0385461060726336E-5 0.0 0.0 4.413820950808693E-5 9 0.12919773196070078 1.0385461060726336E-5 0.0 0.0 4.413820950808693E-5 10-11 0.12921331015229187 1.0385461060726336E-5 0.0 0.0 4.413820950808693E-5 12-13 0.12925744836179998 1.298182632590792E-5 0.0 0.0 4.673457477326851E-5 14-15 0.12928990292761472 1.5578191591089502E-5 0.0 0.0 4.673457477326851E-5 16-17 0.12931456839763394 1.5578191591089502E-5 0.0 0.0 4.673457477326851E-5 18-19 0.12935351387661168 1.5578191591089502E-5 0.0 0.0 4.673457477326851E-5 20-21 0.12937817934663093 1.6876374223680295E-5 0.0 0.0 4.673457477326851E-5 22-23 0.12943400119983234 2.8560017916997422E-5 0.0 1.298182632590792E-6 4.673457477326851E-5 24-25 0.1296624813431683 2.8560017916997422E-5 0.0 2.596365265181584E-6 4.80327574058593E-5 26-27 0.13066597651816098 2.8560017916997422E-5 0.0 2.596365265181584E-6 4.933094003845009E-5 28-29 0.1344268116047765 2.9858200549588212E-5 0.0 2.596365265181584E-6 4.933094003845009E-5 30-31 0.14449291973788553 3.1156383182179005E-5 0.0 5.192730530363168E-6 5.0629122671040886E-5 32-33 0.16736559954150265 3.1156383182179005E-5 0.0 5.192730530363168E-6 5.582185320140405E-5 34-35 0.21057820483255235 3.764729634513296E-5 0.0 5.192730530363168E-6 5.841821846658564E-5 36-37 0.2798933683171051 4.154184424290534E-5 0.0 5.192730530363168E-6 5.971640109917643E-5 38-39 0.38197076690035164 4.413820950808693E-5 0.0 5.192730530363168E-6 5.971640109917643E-5 40-41 0.5209490068149915 4.673457477326851E-5 0.0 5.192730530363168E-6 5.971640109917643E-5 42-43 0.7026932771950698 4.673457477326851E-5 0.0 5.192730530363168E-6 5.971640109917643E-5 44-45 0.9305619765110988 4.80327574058593E-5 0.0 5.192730530363168E-6 5.971640109917643E-5 46-47 1.2077434413087218 5.192730530363168E-5 0.0 6.49091316295396E-6 5.971640109917643E-5 48-49 1.5361044582136043 6.1014583731767224E-5 5.192730530363168E-6 7.789095795544751E-6 5.971640109917643E-5 50-51 1.9133316657744692 6.750549689472118E-5 5.192730530363168E-6 1.298182632590792E-5 6.1014583731767224E-5 52-53 2.3339701005691698 7.659277532285673E-5 5.192730530363168E-6 1.298182632590792E-5 6.620731426213038E-5 54-55 2.8022310670578316 7.789095795544751E-5 5.192730530363168E-6 1.298182632590792E-5 7.010186215990277E-5 56-57 3.3201332392341323 8.17855058532199E-5 5.192730530363168E-6 1.298182632590792E-5 7.010186215990277E-5 58-59 3.881085743872409 8.308368848581068E-5 5.192730530363168E-6 1.4280008958498711E-5 7.010186215990277E-5 60-61 4.481194033074889 8.308368848581068E-5 5.192730530363168E-6 1.9472739488861878E-5 7.010186215990277E-5 62-63 5.114849957868781 8.308368848581068E-5 5.192730530363168E-6 2.077092212145267E-5 7.010186215990277E-5 64-65 5.780703408316189 8.438187111840147E-5 5.192730530363168E-6 2.077092212145267E-5 7.010186215990277E-5 66-67 6.477626263709393 8.568005375099227E-5 5.192730530363168E-6 2.077092212145267E-5 7.010186215990277E-5 68-69 7.203790682901705 8.568005375099227E-5 5.192730530363168E-6 2.077092212145267E-5 7.010186215990277E-5 70-71 7.955713641889881 8.568005375099227E-5 5.192730530363168E-6 2.077092212145267E-5 7.010186215990277E-5 72-73 8.728027155488164 8.568005375099227E-5 5.192730530363168E-6 2.206910475404346E-5 7.269822742508435E-5 74-75 9.519947121386464 8.827641901617384E-5 5.192730530363168E-6 2.3367287386634254E-5 7.529459269026593E-5 76-77 10.330812764624794 9.476733217912781E-5 5.192730530363168E-6 2.4665470019225047E-5 7.529459269026593E-5 78-79 11.160363150494003 9.866188007690019E-5 5.192730530363168E-6 2.596365265181584E-5 7.529459269026593E-5 80-81 12.006601674105166 1.0125824534208177E-4 5.192730530363168E-6 2.596365265181584E-5 7.529459269026593E-5 82-83 12.860888930038394 1.0125824534208177E-4 5.192730530363168E-6 2.596365265181584E-5 7.659277532285673E-5 84-85 13.728921343685492 1.0385461060726336E-4 5.192730530363168E-6 2.596365265181584E-5 8.308368848581068E-5 86-87 14.604401431095763 1.0645097587244493E-4 5.192730530363168E-6 2.596365265181584E-5 8.308368848581068E-5 88 15.268216734810004 1.0904734113762652E-4 5.192730530363168E-6 2.596365265181584E-5 8.308368848581068E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 248780 0.0 87.20959 8 TGGTATC 251515 0.0 86.22551 7 GTGGTAT 254595 0.0 85.96379 6 AGTGGTA 256190 0.0 85.71456 5 GTATCAA 252485 0.0 85.30946 9 GCAGTGG 258805 0.0 85.22774 3 AAGCAGT 259785 0.0 81.474884 1 CAGTGGT 277125 0.0 79.40885 4 AGCAGTG 280770 0.0 78.36624 2 CAACGCA 244195 0.0 45.796555 12-13 ATCAACG 244255 0.0 45.70199 10-11 ACGCAGA 253095 0.0 44.731262 14-15 TCAACGC 244135 0.0 44.07583 12-13 AGAGTAC 255945 0.0 43.992058 18-19 AACGCAG 249015 0.0 43.268944 14-15 TATCAAC 249485 0.0 43.12591 10-11 CGCAGAG 253395 0.0 42.52751 16-17 GAGTACT 253300 0.0 41.959606 20-21 AGTACTT 268540 0.0 41.84044 20-21 CAGAGTA 267810 0.0 39.986088 18-19 >>END_MODULE