FastQCFastQC Report
Thu 26 May 2016
SRR1660684_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660684_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34637183
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21052656.078049130034622No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1440130.4157757286439835No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT527100.15217750242564473No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT366610.10584290298665455No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2632850.087.636535
GGTATCA2644700.087.166158
GTGGTAT2656050.086.622396
TGGTATC2654900.086.338017
GCAGTGG2727650.086.245423
GTATCAA2672650.085.74889
AAGCAGT2813150.083.8135451
AGCAGTG2894450.081.647472
CAGTGGT2858900.081.190024
CAACGCA2613300.045.64993712-13
ATCAACG2619600.045.50101510-11
ACGCAGA2651100.045.13493314-15
AGAGTAC2644600.044.4967118-19
TCAACGC2617000.044.06728412-13
AACGCAG2626850.043.6925514-15
TATCAAC2642700.043.28107510-11
CGCAGAG2649900.043.087116-17
GAGTACT2627900.042.52758820-21
AGTACTT2786300.042.34853720-21
CAGAGTA2705950.041.48146418-19
Produced by FastQC (version 0.11.5)