##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660684_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34637183 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.29645268785282 31.0 31.0 34.0 28.0 34.0 2 31.43527266637128 31.0 31.0 34.0 28.0 34.0 3 31.48491989085833 33.0 31.0 34.0 28.0 34.0 4 35.02174535960387 37.0 35.0 37.0 32.0 37.0 5 34.98912579005054 37.0 35.0 37.0 32.0 37.0 6 35.0360412103952 37.0 35.0 37.0 32.0 37.0 7 34.94697917552937 37.0 35.0 37.0 32.0 37.0 8 34.9062269584683 37.0 35.0 37.0 32.0 37.0 9 36.513070130443346 39.0 37.0 39.0 32.0 39.0 10-11 36.4258496281294 39.0 37.0 39.0 32.0 39.0 12-13 36.47663106725509 39.0 37.0 39.0 32.0 39.0 14-15 37.74043600774347 40.0 37.0 41.0 32.0 41.0 16-17 37.6312674301487 40.0 37.0 41.0 32.0 41.0 18-19 37.57118483047539 40.0 37.0 41.0 32.0 41.0 20-21 37.47270228355464 40.0 37.0 41.0 31.5 41.0 22-23 37.29173374174222 40.0 36.5 41.0 31.0 41.0 24-25 37.31521981738527 40.0 37.0 41.0 31.0 41.0 26-27 37.32195572890555 40.0 37.0 41.0 31.0 41.0 28-29 37.20708593709829 40.0 36.5 41.0 30.5 41.0 30-31 37.02533779955489 40.0 36.0 41.0 30.0 41.0 32-33 36.91622160785997 40.0 36.0 41.0 30.0 41.0 34-35 36.76953275905838 39.5 36.0 41.0 30.0 41.0 36-37 36.53873051685525 39.0 35.5 40.5 29.5 41.0 38-39 36.0971351654088 38.0 35.0 40.0 29.0 41.0 40-41 36.21865668175151 39.0 35.0 40.0 29.0 41.0 42-43 36.097792825704104 39.0 35.0 40.0 28.5 41.0 44-45 35.95021563964944 38.0 35.0 40.0 28.0 41.0 46-47 35.91083462243451 38.0 35.0 40.0 27.5 41.0 48-49 35.97781583450363 39.0 35.0 40.0 28.0 41.0 50-51 34.989231500148264 38.0 34.0 40.0 25.5 40.5 52-53 34.95064142485259 38.0 33.5 39.5 25.5 40.5 54-55 35.07343442161564 38.5 34.0 40.0 23.5 41.0 56-57 34.70962520248832 39.0 34.0 41.0 21.0 41.0 58-59 34.50400698001336 38.0 33.5 41.0 20.0 41.0 60-61 34.34165243749759 38.0 33.0 40.0 20.0 41.0 62-63 34.10630461778604 38.0 33.0 40.0 20.0 41.0 64-65 33.849212333462575 37.0 33.0 40.0 19.0 41.0 66-67 33.57819030779726 37.0 32.5 40.0 19.0 41.0 68-69 33.298288489569146 36.0 32.0 39.5 19.0 41.0 70-71 33.05923266046202 36.0 32.0 39.0 19.0 41.0 72-73 32.76948575754558 35.0 32.0 39.0 19.0 40.5 74-75 32.41316666831711 35.0 31.0 38.0 18.5 40.0 76-77 32.02914217937411 35.0 31.0 37.0 18.0 39.5 78-79 31.674829041380185 35.0 31.0 37.0 17.0 39.0 80-81 31.315174374890706 35.0 30.5 36.0 15.5 39.0 82-83 30.989851411992714 35.0 30.0 36.0 14.5 37.5 84-85 30.680429742222397 35.0 30.0 35.0 12.5 37.0 86-87 30.405758098168665 34.0 30.0 35.0 11.0 37.0 88-89 30.180657936299266 34.0 29.0 35.0 10.5 36.0 90-91 29.968771767611702 34.0 29.0 35.0 10.0 36.0 92-93 29.816330170383658 34.0 29.0 35.0 10.0 36.0 94-95 29.687453076077233 34.0 29.0 35.0 10.0 35.5 96-97 29.516047422216754 34.0 29.0 35.0 10.0 35.0 98-99 29.380534410087563 34.0 29.0 35.0 10.0 35.0 100 29.273777085163076 34.0 28.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5840959269604724 1101 2 0.504060398014083 1101 3 0.5566266251029965 1101 4 0.46083395111777037 1101 5 0.5372018463164423 1101 6 0.5466259978579231 1101 7 0.5756888984019994 1101 8 0.6552244604991841 1101 9 0.6907912166026833 1101 10-11 0.7042207215698255 1101 12-13 0.6235193200294731 1101 14-15 0.6471934418075946 1101 16-17 0.6650443042261216 1101 18-19 0.7047386282028754 1101 20-21 0.7378673999147765 1101 22-23 0.6902491157001904 1101 24-25 0.6699383682449778 1101 26-27 0.6506191990865915 1101 28-29 0.654572154370392 1101 30-31 0.5457349185365103 1101 32-33 0.5890642097300045 1101 34-35 0.5296764472544595 1101 36-37 0.5695338107560133 1101 38-39 0.5809480782320762 1101 40-41 0.5481039969602506 1101 42-43 0.5178162983350347 1101 44-45 0.5390711423845218 1101 46-47 0.5332021697506022 1101 48-49 0.6007504541550617 1101 50-51 0.5882185664593891 1101 52-53 0.5894148643455566 1101 54-55 0.6559863971298086 1101 56-57 0.6051999049238219 1101 58-59 0.5481143976030083 1101 60-61 0.44929581644639427 1101 62-63 0.48600705484183493 1101 64-65 0.4159174224279383 1101 66-67 0.43338190378332087 1101 68-69 0.3344711577006052 1101 70-71 0.3406244873530113 1101 72-73 0.31982829095960597 1101 74-75 0.2723342382173968 1101 76-77 0.32062495714079375 1101 78-79 0.2389160054407249 1101 80-81 0.22935560302906666 1101 82-83 0.18348723290921853 1101 84-85 0.14432406777166307 1101 86-87 0.18147045421315866 1101 88-89 0.12301014772633678 1101 90-91 0.13983543544905075 1101 92-93 0.11715057139625529 1101 94-95 0.00976094811663586 1101 96-97 0.015834900148401942 1101 98-99 0.05860443969459794 1101 100 0.07172421144743168 1102 1 0.4815735934303973 1102 2 0.43877486807121 1102 3 0.4708863327599353 1102 4 0.4073697307031239 1102 5 0.3570229536893734 1102 6 0.40372575296502333 1102 7 0.4967417319355647 1102 8 0.5878157140323381 1102 9 0.547001261693346 1102 10-11 0.5435158537890885 1102 12-13 0.48106677706719125 1102 14-15 0.5737290639448815 1102 16-17 0.6419694089436447 1102 18-19 0.5426006891615742 1102 20-21 0.5033607820629769 1102 22-23 0.5353803571941356 1102 24-25 0.5048440423039082 1102 26-27 0.4576695636190564 1102 28-29 0.4576543644406996 1102 30-31 0.49401700822621564 1102 32-33 0.44494845524982196 1102 34-35 0.4437921327809562 1102 36-37 0.48715292000047583 1102 38-39 0.473554535487267 1102 40-41 0.44218102568537887 1102 42-43 0.4240270484811717 1102 44-45 0.3931539671477182 1102 46-47 0.42966089608036384 1102 48-49 0.49145723739248126 1102 50-51 0.43467773951277877 1102 52-53 0.43037948950914995 1102 54-55 0.531778852072371 1102 56-57 0.5300579504047818 1102 58-59 0.4533776000553118 1102 60-61 0.40965069380909824 1102 62-63 0.4338312224216452 1102 64-65 0.31253684426158657 1102 66-67 0.2436263349703367 1102 68-69 0.34972173703676646 1102 70-71 0.40827984592120004 1102 72-73 0.32702920360537746 1102 74-75 0.34951355431226716 1102 76-77 0.3081336344611856 1102 78-79 0.24719160365417991 1102 80-81 0.302125535057872 1102 82-83 0.2464455526039906 1102 84-85 0.21500660917578074 1102 86-87 0.20745760527178803 1102 88-89 0.22708543192918995 1102 90-91 0.1801984344827119 1102 92-93 0.1095856897564218 1102 94-95 0.08085310961267567 1102 96-97 0.12717167743074143 1102 98-99 0.0883629134398447 1102 100 0.08614156130950335 1103 1 0.4831903107149138 1103 2 0.4555665116646672 1103 3 0.38010846021596834 1103 4 0.39612155138100746 1103 5 0.4606199807991871 1103 6 0.4361693750664202 1103 7 0.41291730648318037 1103 8 0.5177407902076894 1103 9 0.563422163703116 1103 10-11 0.5467046086736858 1103 12-13 0.5782996091110633 1103 14-15 0.6024132314557704 1103 16-17 0.6431288639308974 1103 18-19 0.6487094628722829 1103 20-21 0.633591369349098 1103 22-23 0.6297785246117513 1103 24-25 0.5422025682435319 1103 26-27 0.5159135748970769 1103 28-29 0.5232596674034653 1103 30-31 0.5406404877450939 1103 32-33 0.50769995731698 1103 34-35 0.5057875541628221 1103 36-37 0.48470065272264407 1103 38-39 0.4725156092493563 1103 40-41 0.47596121188092155 1103 42-43 0.43410320424798243 1103 44-45 0.40773846741926434 1103 46-47 0.422305635417473 1103 48-49 0.3981089152148556 1103 50-51 0.3492197930449734 1103 52-53 0.35655203209907427 1103 54-55 0.4696219932754033 1103 56-57 0.4747109282514046 1103 58-59 0.40613420076464024 1103 60-61 0.3764317638802339 1103 62-63 0.3438477992720266 1103 64-65 0.30143150317216794 1103 66-67 0.26874818936273215 1103 68-69 0.2120993199052137 1103 70-71 0.2805082195321589 1103 72-73 0.24846944580639274 1103 74-75 0.28425577227653065 1103 76-77 0.2696651229911069 1103 78-79 0.23156177896403918 1103 80-81 0.18836665339814118 1103 82-83 0.17455377203445366 1103 84-85 0.10091166064054136 1103 86-87 0.09472065377837069 1103 88-89 0.09063333214425029 1103 90-91 0.06855301114785561 1103 92-93 0.027966564496775703 1103 94-95 0.062241222122054296 1103 96-97 0.10187220251709661 1103 98-99 0.09269198704233617 1103 100 0.05737550311681616 1104 1 0.4342152280550202 1104 2 0.4314467023456281 1104 3 0.3820382902996151 1104 4 0.353039384588115 1104 5 0.4148436278005434 1104 6 0.3900667734173666 1104 7 0.4495632351047547 1104 8 0.4998949616304884 1104 9 0.5601973171007586 1104 10-11 0.5344201487496605 1104 12-13 0.483007997889878 1104 14-15 0.48359940378009725 1104 16-17 0.5496364106566247 1104 18-19 0.517050504125038 1104 20-21 0.441498973578625 1104 22-23 0.5361400671293168 1104 24-25 0.49375949335710345 1104 26-27 0.38313134490440603 1104 28-29 0.4201129368873566 1104 30-31 0.4270544097021727 1104 32-33 0.39722184288505247 1104 34-35 0.41709514579819995 1104 36-37 0.44143032233073853 1104 38-39 0.4236275772050675 1104 40-41 0.3946282399589194 1104 42-43 0.3721636102722172 1104 44-45 0.34798230155458754 1104 46-47 0.39179639248947495 1104 48-49 0.43553329081953507 1104 50-51 0.3736246459504571 1104 52-53 0.4460073961257365 1104 54-55 0.492812123487262 1104 56-57 0.4351856884487333 1104 58-59 0.42611288007923775 1104 60-61 0.39300295013482867 1104 62-63 0.4347919616500491 1104 64-65 0.3559123447448229 1104 66-67 0.3075991185669764 1104 68-69 0.40362662144741535 1104 70-71 0.411390428788188 1104 72-73 0.3989077420357603 1104 74-75 0.3327481161915742 1104 76-77 0.3207080587255149 1104 78-79 0.3202391198926584 1104 80-81 0.2936629890409499 1104 82-83 0.2717004994967205 1104 84-85 0.24673957359878784 1104 86-87 0.23196933425029087 1104 88-89 0.22525597745036663 1104 90-91 0.2724435200002482 1104 92-93 0.28756167112939224 1104 94-95 0.23741910268291377 1104 96-97 0.20170874462634814 1104 98-99 0.1539909501574641 1104 100 0.19804582020262984 1105 1 0.4512822820371447 1105 2 0.38458522621865043 1105 3 0.4005355107410722 1105 4 0.31945921912601705 1105 5 0.4061053354098121 1105 6 0.36225202053348227 1105 7 0.3941690820387862 1105 8 0.46973225807009555 1105 9 0.46713266459831004 1105 10-11 0.4682424488718411 1105 12-13 0.46702068490242965 1105 14-15 0.5065754708506987 1105 16-17 0.511627847941277 1105 18-19 0.4830463424061193 1105 20-21 0.5548867535079367 1105 22-23 0.5234091821797406 1105 24-25 0.48687089483735946 1105 26-27 0.4700770263149323 1105 28-29 0.4627485078786435 1105 30-31 0.46144832758059806 1105 32-33 0.43037725918707537 1105 34-35 0.48866253038256957 1105 36-37 0.505177451328457 1105 38-39 0.4264051781989977 1105 40-41 0.4188710400494742 1105 42-43 0.4196714805642898 1105 44-45 0.44621936545492247 1105 46-47 0.3230157506018472 1105 48-49 0.43783222506505126 1105 50-51 0.28325137973244807 1105 52-53 0.41032546963845107 1105 54-55 0.5076193650487753 1105 56-57 0.4980882397134039 1105 58-59 0.4489370307016216 1105 60-61 0.4028691936161053 1105 62-63 0.44510602713326364 1105 64-65 0.37544893599385887 1105 66-67 0.433228770349416 1105 68-69 0.4893381681204616 1105 70-71 0.4541113304348201 1105 72-73 0.3983822738491867 1105 74-75 0.3644808367775809 1105 76-77 0.3313123708443868 1105 78-79 0.31151916433940485 1105 80-81 0.33624062534070376 1105 82-83 0.3173909001773616 1105 84-85 0.22444740013310138 1105 86-87 0.3295794531768479 1105 88-89 0.3116364762416879 1105 90-91 0.35495515009691303 1105 92-93 0.32376752113720997 1105 94-95 0.2682687988951926 1105 96-97 0.3225958344833906 1105 98-99 0.3297585381889263 1105 100 0.2740590499661373 1106 1 0.36155034551352117 1106 2 0.37218203404499306 1106 3 0.3292840463834068 1106 4 0.2514579132849022 1106 5 0.32718978771558227 1106 6 0.335930118189097 1106 7 0.3522017037503886 1106 8 0.43183099969209593 1106 9 0.4554257026389408 1106 10-11 0.3831880077030263 1106 12-13 0.3584938039644143 1106 14-15 0.4235903023936629 1106 16-17 0.5090205663875977 1106 18-19 0.43029551848328396 1106 20-21 0.4792809327529781 1106 22-23 0.4536359096814593 1106 24-25 0.40643086635579806 1106 26-27 0.3848335163123764 1106 28-29 0.39360896278292756 1106 30-31 0.3827248710647808 1106 32-33 0.34767062458563913 1106 34-35 0.39436667676908144 1106 36-37 0.41506074426715855 1106 38-39 0.3932802798705879 1106 40-41 0.3633368078896595 1106 42-43 0.329767783650027 1106 44-45 0.35294022931298485 1106 46-47 0.35825509248343224 1106 48-49 0.3901404629873966 1106 50-51 0.3701456824502216 1106 52-53 0.2660639467837882 1106 54-55 0.2838335939210026 1106 56-57 0.3414724450436637 1106 58-59 0.35095054386887625 1106 60-61 0.3761842284079364 1106 62-63 0.3781733684364852 1106 64-65 0.35053978903204097 1106 66-67 0.2941522980398119 1106 68-69 0.33006751403863177 1106 70-71 0.387919364081057 1106 72-73 0.3229821556823893 1106 74-75 0.30985326205075836 1106 76-77 0.283091656100126 1106 78-79 0.24184373002458415 1106 80-81 0.20360148838277325 1106 82-83 0.18177739383517277 1106 84-85 0.19077865169352037 1106 86-87 0.2290108100665229 1106 88-89 0.2303083562104007 1106 90-91 0.2750694847805235 1106 92-93 0.2692076472929905 1106 94-95 0.1997914603758204 1106 96-97 0.25071345368469267 1106 98-99 0.2630623814013262 1106 100 0.250123934110384 1107 1 0.3866312955599618 1107 2 0.2267830652653906 1107 3 0.34478670487479945 1107 4 0.3030458423325655 1107 5 0.3747064741621742 1107 6 0.3781248087444169 1107 7 0.3799111276812184 1107 8 0.45325019698109514 1107 9 0.4992196248721612 1107 10-11 0.41885473936791584 1107 12-13 0.48538121365597675 1107 14-15 0.42528433136347843 1107 16-17 0.4945865544346262 1107 18-19 0.4792268789969114 1107 20-21 0.45401858007499385 1107 22-23 0.46432225608496225 1107 24-25 0.4512445029315444 1107 26-27 0.393381221793085 1107 28-29 0.3996837550395256 1107 30-31 0.4040566800556036 1107 32-33 0.33038105075764435 1107 34-35 0.3365040536749362 1107 36-37 0.4223433402891672 1107 38-39 0.38818369394336116 1107 40-41 0.41537222504235416 1107 42-43 0.4184052970420069 1107 44-45 0.4760139431084056 1107 46-47 0.34723639265988737 1107 48-49 0.3909592057980831 1107 50-51 0.31372792994574183 1107 52-53 0.3044564623251489 1107 54-55 0.3766590783626711 1107 56-57 0.3937875997181379 1107 58-59 0.34880835052037895 1107 60-61 0.30474070353051275 1107 62-63 0.3517265662176001 1107 64-65 0.3302821289879887 1107 66-67 0.3210850784604915 1107 68-69 0.33170551464056786 1107 70-71 0.36481623382839956 1107 72-73 0.33890172458375645 1107 74-75 0.3154171505393393 1107 76-77 0.31316567012849106 1107 78-79 0.2941560761743638 1107 80-81 0.26516244159779134 1107 82-83 0.23671250098438534 1107 84-85 0.2265644878058808 1107 86-87 0.2595819445379597 1107 88-89 0.30628744377497696 1107 90-91 0.330357206074396 1107 92-93 0.2846420795373703 1107 94-95 0.30595699336735294 1107 96-97 0.3123013891334523 1107 98-99 0.36496221346232716 1107 100 0.30199781697276507 1108 1 0.23030591483441398 1108 2 0.13240295511502254 1108 3 0.2378512444484251 1108 4 0.1966948079876687 1108 5 0.23756851855393535 1108 6 0.26638877118521975 1108 7 0.28189342446680854 1108 8 0.3975450490810033 1108 9 0.3804217623319346 1108 10-11 0.37497458323777266 1108 12-13 0.28358927683633084 1108 14-15 0.327913087049275 1108 16-17 0.3882838199479366 1108 18-19 0.34051071765188823 1108 20-21 0.35306614902362554 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-5.285468915291389 2316 56-57 -3.0864525415113278 2316 58-59 -4.881715289302633 2316 60-61 -2.335476852040088 2316 62-63 -2.8912526268418155 2316 64-65 -1.9734311929527344 2316 66-67 -1.586193547663271 2316 68-69 -1.5023983625410473 2316 70-71 -1.488252814613709 2316 72-73 -1.3355673667587737 2316 74-75 -1.4452200261290216 2316 76-77 -1.4904935350614892 2316 78-79 -1.4549772351427137 2316 80-81 -1.4209972808064002 2316 82-83 -1.470435020599421 2316 84-85 -1.2554531304679486 2316 86-87 -1.2280688982955432 2316 88-89 -1.3906969281187074 2316 90-91 -2.2692830213879844 2316 92-93 -1.4686704345979145 2316 94-95 -1.3745502791195179 2316 96-97 -1.5747291746942942 2316 98-99 -1.6067734486305199 2316 100 -1.6254540695609627 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 0.0 4 2.0 5 47.0 6 461.0 7 5419.0 8 17728.0 9 34504.0 10 46169.0 11 50492.0 12 54912.0 13 65580.0 14 82728.0 15 107202.0 16 134056.0 17 163022.0 18 187239.0 19 212685.0 20 240200.0 21 288017.0 22 366460.0 23 496063.0 24 665972.0 25 769494.0 26 721003.0 27 641750.0 28 609710.0 29 648606.0 30 742812.0 31 888607.0 32 1089115.0 33 1376554.0 34 1825357.0 35 2628727.0 36 3925418.0 37 6240855.0 38 7142049.0 39 2165134.0 40 3031.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.81855089374606 36.59018004724902 27.840161136785074 18.75110792221985 2 19.369751105308914 34.67263636599067 28.0660479837324 17.891564544968013 3 25.764211845143215 27.030190898826216 27.51862475632512 19.686972499705444 4 18.673760438814554 27.337818812306104 27.252895065400523 26.735525683478816 5 19.36471237123385 34.185708504055796 27.091201088697265 19.35837803601309 6 26.60850788685391 26.970033200208377 27.309079747133797 19.11237916580391 7 19.420568202244212 27.035513925010164 34.375379469468264 19.168538403277356 8 26.38713022138034 27.140612527400833 27.30676348133106 19.16549376988777 9 26.050932150103794 26.950670549700366 27.760667229857262 19.23773007033858 10-11 18.869426178057846 31.04270802682272 30.999993171744077 19.08787262337536 12-13 18.899480110603708 27.48578225562564 30.781870923364853 22.8328667104058 14-15 18.90617772849355 34.74455383652613 27.156367026519856 19.192901408460465 16-17 22.681802079179107 27.321061173586607 27.10943741825168 22.887699328982603 18-19 19.172601402972383 31.0863039494416 27.109998145056576 22.631096502529434 20-21 22.68960483220961 31.165472619141365 27.06607585860593 19.078846690043093 22-23 22.65991567546206 27.574530183187118 30.64689425668754 19.118659884663284 24-25 19.041584476708156 30.995858638281774 27.086743430575407 22.87581345443466 26-27 19.01389538421282 27.563309462070208 34.30696403902263 19.11583111469434 28-29 19.0625150694563 27.534423855412015 34.313117519150005 19.089943555981677 30-31 19.088105929743662 27.551911568499698 34.271971941311875 19.088010560444765 32-33 19.14973307065212 27.5685239161711 34.18370074530532 19.09804226787146 34-35 19.15903633263295 27.67191281094325 34.08909228169496 19.079958574728835 36-37 19.14356858304166 27.661742498957175 34.09855545480204 19.096133463199124 38-39 19.181149408588368 27.648751387345786 34.019145011003204 19.15095419306264 40-41 19.24126985208514 27.712372459364442 33.94254722457973 19.103810463970692 42-43 19.278355109543597 27.75894763268297 33.96627296035321 18.996424297420226 44-45 19.40538606495335 27.826991773813464 33.7624243121346 19.00519784909859 46-47 19.32549776115098 27.880851900716326 33.75998073042943 19.033669607703256 48-49 19.46498701163196 27.895041346520273 33.58991719638391 19.05005444546386 50-51 19.50089434776637 27.95017510866621 33.51011551353839 19.03881503002903 52-53 19.60545775733025 28.179268653961792 33.23689554271356 18.978378045994397 54-55 20.045095379719584 28.414701503833335 32.32901527970452 19.211187836742564 56-57 20.583130546109217 29.08340328963488 30.997838964204576 19.335627200051324 58-59 20.88814368960188 29.540120292820166 30.245682058369777 19.326053959208178 60-61 21.020163450539915 29.923320419270887 29.76996559632447 19.286550533864723 62-63 21.227010271609142 30.099834147972608 29.46793970241967 19.205215877998576 64-65 21.470345946663038 30.176031692249506 29.24891579995037 19.104706561137085 66-67 21.693512008673377 29.98664828822621 29.342458998773758 18.977380704326656 68-69 21.88787206232606 29.69563767841975 29.566210550737853 18.85027970851634 70-71 21.989879438168032 29.496240433851735 29.798086774684606 18.715793353295624 72-73 22.11711594474481 29.324548755462708 29.88105235639643 18.67728294339605 74-75 22.262884080118518 29.34606733697357 29.764793723784326 18.626254859123588 76-77 22.58456958604931 29.387928983557327 29.462587305462744 18.56491412493062 78-79 22.834085402975326 29.556902182410994 29.09437414821853 18.514638266395146 80-81 23.133581145961852 29.71476874403467 28.690818374411997 18.460831735591483 82-83 23.400083789239705 29.892388074287346 28.25349743456893 18.454030701904024 84-85 23.56013401669116 30.021000728018493 27.962919263811624 18.45594599147872 86-87 23.771856984600888 30.021600229636146 27.69174388492428 18.51479890083868 88-89 24.04469238565754 30.028707942801635 27.474716084837823 18.451883586702994 90-91 24.28494886348802 29.95454541906074 27.356071408528198 18.404434308923047 92-93 24.45141929114633 29.83932718350249 27.388740675926282 18.3205128494249 94-95 24.621373541209444 29.825207003955413 27.28837485492849 18.265044599906652 96-97 24.740232334486166 29.876445889038894 27.141295430538463 18.242026345936477 98-99 24.822833426646863 29.89512968132914 27.006190783660767 18.27584610836323 100 24.858482512057485 29.93076439651806 26.872043304891935 18.33870978653252 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 121373.0 1 71873.5 2 18311.0 3 13734.5 4 13447.5 5 14078.5 6 14789.5 7 14980.5 8 15007.5 9 15526.0 10 16781.5 11 22416.5 12 176134.0 13 207782.0 14 87273.0 15 90488.5 16 103948.0 17 118373.0 18 132974.0 19 147553.0 20 164949.0 21 192182.5 22 227898.0 23 271023.0 24 324037.0 25 378559.0 26 433476.0 27 499433.0 28 586551.0 29 681862.0 30 761837.0 31 835618.0 32 897243.5 33 917537.0 34 930322.0 35 968703.0 36 1022292.5 37 1083186.0 38 1130965.0 39 1128365.5 40 1100335.0 41 1104498.5 42 1152507.5 43 1157999.0 44 1139431.0 45 1158381.0 46 1177109.5 47 1191405.5 48 1147995.5 49 1068651.5 50 1028027.0 51 993650.0 52 952066.0 53 896525.5 54 808664.0 55 711465.0 56 611849.0 57 516522.0 58 438432.0 59 362758.0 60 288650.0 61 242180.0 62 194150.0 63 127033.5 64 75610.5 65 48244.5 66 32803.0 67 25062.0 68 19099.5 69 14122.0 70 10823.5 71 8487.5 72 7230.5 73 6400.0 74 5785.5 75 5366.0 76 4143.0 77 2469.5 78 1526.5 79 1251.5 80 1060.0 81 862.5 82 724.0 83 612.0 84 507.0 85 421.0 86 362.0 87 312.0 88 262.5 89 215.0 90 174.5 91 159.0 92 143.5 93 117.5 94 95.5 95 80.5 96 59.5 97 34.5 98 27.0 99 25.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.939444729093587E-4 2 0.0014031164139416301 3 0.0010220230669451382 4 6.582521448121228E-4 5 0.0016080984414927738 6 1.472406113395538E-4 7 2.5983637295215374E-5 8 1.7322424863476917E-5 9 1.1548283242317945E-5 10-11 0.004883480276095201 12-13 2.309656648463589E-5 14-15 5.7741416211589724E-6 16-17 1.0104747837028203E-5 18-19 2.309656648463589E-5 20-21 0.0012717546920602636 22-23 0.016244103915725477 24-25 0.02691183056081668 26-27 0.03728796305403936 28-29 0.046893247640837306 30-31 0.10062163542572154 32-33 0.012499573074403886 34-35 0.006599843872984705 36-37 0.001151941253421215 38-39 0.002601250800332117 40-41 0.002225931594956784 42-43 0.0014247694450209764 44-45 0.001027797208566297 46-47 5.456563831995229E-4 48-49 6.625827510279921E-4 50-51 0.18541490513244105 52-53 0.29937336416763455 54-55 0.1604965969663295 56-57 0.1859562309094247 58-59 0.18442897045062817 60-61 0.18221891774512958 62-63 0.18272704220779157 64-65 0.17511094940948288 66-67 0.18286417807129407 68-69 0.16375465637606845 70-71 0.17223109627592983 72-73 0.1653223358262131 74-75 0.15825478648191454 76-77 0.1509678197560119 78-79 0.14679888950553513 80-81 0.14987650698961286 82-83 0.14551703006563785 84-85 0.134870956451626 86-87 0.13514378464322574 88-89 0.13800487181651 90-91 0.14790463762598707 92-93 0.14309766472637223 94-95 0.1429056745174687 96-97 0.14081687878601443 98-99 0.13771616473545206 100 0.1370348160241553 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.4637183E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.8622049008525 #Duplication Level Percentage of deduplicated Percentage of total 1 69.81914255457954 15.962195430846279 2 12.619299597468403 5.770100262051363 3 5.227441514219069 3.5853251701589706 4 2.595769897805412 2.3738009311636916 5 1.6604944762201397 1.8981282476039294 6 1.104255535667226 1.5147429791594853 7 0.757465355681527 1.2122129716821732 8 0.6484883404045636 1.1860698651314334 9 0.48440327664785165 0.9967074268830771 >10 4.118751088475701 18.800784853911573 >50 0.5361099164635119 8.648858941423734 >100 0.36389565627082054 15.417705690415362 >500 0.04132459923467376 7.208330156090444 >1k 0.022917785336347755 8.238162075564723 >5k 1.5183506845024576E-4 0.24973288125338172 >10k+ 8.857045658646926E-5 6.93714211666033 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2105265 6.078049130034622 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 144013 0.4157757286439835 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 52710 0.15217750242564473 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 36661 0.10584290298665455 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.12250996277612992 0.0 0.0 0.0 0.0 2 0.12272071894530222 0.0 0.0 0.0 0.0 3 0.12276113793665033 0.0 0.0 2.8870708105794862E-6 0.0 4 0.1227928957155667 0.0 0.0 5.7741416211589724E-6 0.0 5 0.12279578278637729 2.8870708105794862E-6 0.0 5.7741416211589724E-6 0.0 6 0.12291126561880046 2.8870708105794862E-6 0.0 5.7741416211589724E-6 0.0 7 0.12291703976042162 2.8870708105794862E-6 0.0 5.7741416211589724E-6 0.0 8 0.12292281390204278 2.8870708105794862E-6 0.0 5.7741416211589724E-6 0.0 9 0.12292281390204278 2.8870708105794862E-6 0.0 5.7741416211589724E-6 0.0 10-11 0.12293291864987982 2.8870708105794862E-6 2.8870708105794862E-6 5.7741416211589724E-6 0.0 12-13 0.12298921653068612 2.8870708105794862E-6 2.8870708105794862E-6 8.661212431738459E-6 0.0 14-15 0.12304262734068183 2.8870708105794862E-6 2.8870708105794862E-6 1.1548283242317945E-5 0.0 16-17 0.12307294158419292 2.8870708105794862E-6 2.8870708105794862E-6 1.1548283242317945E-5 2.8870708105794862E-6 18-19 0.12314078774824154 2.8870708105794862E-6 2.8870708105794862E-6 1.1548283242317945E-5 2.8870708105794862E-6 20-21 0.12317687613337377 2.8870708105794862E-6 2.8870708105794862E-6 1.2991818647607689E-5 2.8870708105794862E-6 22-23 0.12327936714714935 2.8870708105794862E-6 2.8870708105794862E-6 1.4435354052897431E-5 2.8870708105794862E-6 24-25 0.12384523302602293 2.8870708105794862E-6 2.8870708105794862E-6 1.4435354052897431E-5 2.8870708105794862E-6 26-27 0.1262530500820462 2.8870708105794862E-6 2.8870708105794862E-6 1.4435354052897431E-5 2.8870708105794862E-6 28-29 0.1337449988355 4.330606215869229E-6 2.8870708105794862E-6 1.4435354052897431E-5 2.8870708105794862E-6 30-31 0.1520822290888956 5.7741416211589724E-6 2.8870708105794862E-6 1.7322424863476917E-5 2.8870708105794862E-6 32-33 0.1889703328356697 5.7741416211589724E-6 2.8870708105794862E-6 1.876596026876666E-5 2.8870708105794862E-6 34-35 0.25136137658769764 8.661212431738459E-6 2.8870708105794862E-6 2.0209495674056405E-5 2.8870708105794862E-6 36-37 0.34658996373925677 8.661212431738459E-6 2.8870708105794862E-6 2.1653031079346146E-5 5.7741416211589724E-6 38-39 0.4813800822081865 8.661212431738459E-6 2.8870708105794862E-6 2.309656648463589E-5 8.661212431738459E-6 40-41 0.6589334935234196 8.661212431738459E-6 2.8870708105794862E-6 2.309656648463589E-5 8.661212431738459E-6 42-43 0.8824707251741575 8.661212431738459E-6 2.8870708105794862E-6 2.309656648463589E-5 8.661212431738459E-6 44-45 1.1544544485618244 8.661212431738459E-6 2.8870708105794862E-6 2.309656648463589E-5 8.661212431738459E-6 46-47 1.477767403890784 8.661212431738459E-6 2.8870708105794862E-6 2.309656648463589E-5 8.661212431738459E-6 48-49 1.8534272836217656 8.661212431738459E-6 2.8870708105794862E-6 2.5983637295215378E-5 8.661212431738459E-6 50-51 2.2775885094350774 8.661212431738459E-6 2.8870708105794862E-6 2.5983637295215378E-5 8.661212431738459E-6 52-53 2.7467274114064066 8.661212431738459E-6 2.8870708105794862E-6 2.5983637295215378E-5 8.661212431738459E-6 54-55 3.262478071614542 8.661212431738459E-6 2.8870708105794862E-6 2.5983637295215378E-5 8.661212431738459E-6 56-57 3.830164248634192 1.1548283242317945E-5 2.8870708105794862E-6 2.5983637295215378E-5 1.1548283242317945E-5 58-59 4.4394617772467235 1.1548283242317945E-5 2.8870708105794862E-6 2.8870708105794862E-5 1.1548283242317945E-5 60-61 5.091838732959317 1.1548283242317945E-5 2.8870708105794862E-6 3.175777891637435E-5 1.1548283242317945E-5 62-63 5.77380816448035 1.1548283242317945E-5 2.8870708105794862E-6 3.320131432166409E-5 1.1548283242317945E-5 64-65 6.484788327041492 1.1548283242317945E-5 2.8870708105794862E-6 3.4644849726953835E-5 1.1548283242317945E-5 66-67 7.2217434656854165 1.2991818647607689E-5 2.8870708105794862E-6 3.4644849726953835E-5 1.1548283242317945E-5 68-69 7.985448758924766 1.4435354052897431E-5 2.8870708105794862E-6 3.4644849726953835E-5 1.2991818647607689E-5 70-71 8.775251728756349 1.4435354052897431E-5 2.8870708105794862E-6 3.753192053753332E-5 1.4435354052897431E-5 72-73 9.58506354284065 1.4435354052897431E-5 2.8870708105794862E-6 4.041899134811281E-5 1.4435354052897431E-5 74-75 10.41184267207873 1.4435354052897431E-5 2.8870708105794862E-6 4.041899134811281E-5 1.4435354052897431E-5 76-77 11.253832333882348 1.5878889458187173E-5 2.8870708105794862E-6 4.041899134811281E-5 1.4435354052897431E-5 78-79 12.11266083618867 1.7322424863476917E-5 2.8870708105794862E-6 4.041899134811281E-5 1.4435354052897431E-5 80-81 12.985002273423909 2.1653031079346146E-5 2.8870708105794862E-6 4.041899134811281E-5 1.4435354052897431E-5 82-83 13.869026242694158 2.5983637295215378E-5 2.8870708105794862E-6 4.041899134811281E-5 1.4435354052897431E-5 84-85 14.760319856265447 2.5983637295215378E-5 2.8870708105794862E-6 4.041899134811281E-5 1.4435354052897431E-5 86-87 15.6595991076988 3.753192053753332E-5 2.8870708105794862E-6 4.041899134811281E-5 1.4435354052897431E-5 88 16.338877789224373 4.330606215869229E-5 2.8870708105794862E-6 4.041899134811281E-5 1.4435354052897431E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 263285 0.0 87.63653 5 GGTATCA 264470 0.0 87.16615 8 GTGGTAT 265605 0.0 86.62239 6 TGGTATC 265490 0.0 86.33801 7 GCAGTGG 272765 0.0 86.24542 3 GTATCAA 267265 0.0 85.7488 9 AAGCAGT 281315 0.0 83.813545 1 AGCAGTG 289445 0.0 81.64747 2 CAGTGGT 285890 0.0 81.19002 4 CAACGCA 261330 0.0 45.649937 12-13 ATCAACG 261960 0.0 45.501015 10-11 ACGCAGA 265110 0.0 45.134933 14-15 AGAGTAC 264460 0.0 44.49671 18-19 TCAACGC 261700 0.0 44.067284 12-13 AACGCAG 262685 0.0 43.69255 14-15 TATCAAC 264270 0.0 43.281075 10-11 CGCAGAG 264990 0.0 43.0871 16-17 GAGTACT 262790 0.0 42.527588 20-21 AGTACTT 278630 0.0 42.348537 20-21 CAGAGTA 270595 0.0 41.481464 18-19 >>END_MODULE