FastQCFastQC Report
Thu 26 May 2016
SRR1660684_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660684_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34637183
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17712915.113842543142149No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1507830.43532119803160674No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT489810.1414116153729938No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT352800.10185585819724428No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2282050.086.872868
AGTGGTA2310700.086.6985865
GTGGTAT2332700.085.6755756
TGGTATC2314800.085.6396947
GTATCAA2308050.085.199159
GCAGTGG2359250.085.193143
AAGCAGT2360350.081.715971
CAGTGGT2515450.079.7484
AGCAGTG2524050.079.5489652
CAACGCA2230100.045.78944412-13
ATCAACG2233400.045.62961210-11
ACGCAGA2300450.044.93877414-15
AGAGTAC2339700.044.01855518-19
TCAACGC2230400.043.994612-13
AACGCAG2267350.043.3340814-15
TATCAAC2277350.043.11150410-11
CGCAGAG2304250.042.61369716-17
GAGTACT2309750.042.04276720-21
AGTACTT2468700.041.71367320-21
CAGAGTA2398600.040.70171718-19
Produced by FastQC (version 0.11.5)