##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660684_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34637183 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.606818314295364 31.0 30.0 34.0 16.0 34.0 2 30.629459214393965 31.0 31.0 34.0 25.0 34.0 3 31.40587454816981 31.0 31.0 34.0 28.0 34.0 4 35.14461346351405 37.0 35.0 37.0 32.0 37.0 5 35.41406716013828 37.0 35.0 37.0 33.0 37.0 6 35.56030321518929 37.0 35.0 37.0 33.0 37.0 7 35.588793984776416 37.0 35.0 37.0 33.0 37.0 8 35.61994657590948 37.0 35.0 37.0 33.0 37.0 9 37.274648922806456 39.0 37.0 39.0 34.0 39.0 10-11 37.21932913828472 39.0 37.0 39.0 33.5 39.0 12-13 37.042558758314726 39.0 37.0 39.0 33.5 39.0 14-15 38.372517519683974 40.0 38.0 41.0 33.0 41.0 16-17 38.490041612217716 40.0 38.0 41.0 34.0 41.0 18-19 38.48101719184265 40.0 38.0 41.0 34.0 41.0 20-21 38.42282607104625 40.0 38.0 41.0 34.0 41.0 22-23 38.23554546857925 40.0 37.5 41.0 33.5 41.0 24-25 38.25325197490801 40.0 38.0 41.0 34.0 41.0 26-27 38.17618314976711 40.0 38.0 41.0 33.0 41.0 28-29 38.089610823720854 40.0 37.5 41.0 33.0 41.0 30-31 37.97764705634404 40.0 37.0 41.0 33.0 41.0 32-33 37.846739759408266 40.0 37.0 41.0 33.0 41.0 34-35 37.66387113524792 40.0 37.0 41.0 32.0 41.0 36-37 37.470929780865845 39.5 37.0 41.0 32.0 41.0 38-39 37.04965740141166 39.0 35.0 41.0 31.0 41.0 40-41 36.904960544857246 39.0 35.0 40.0 31.0 41.0 42-43 36.74343541447929 39.0 35.0 40.0 31.0 41.0 44-45 36.87622555795025 39.0 35.0 40.5 31.0 41.0 46-47 36.805589949390516 39.0 35.0 41.0 31.0 41.0 48-49 36.688869761723986 39.0 35.0 40.5 30.5 41.0 50-51 36.49605471668987 39.0 35.0 40.0 30.0 41.0 52-53 36.32957222589378 39.0 35.0 40.0 30.0 41.0 54-55 36.01382495510677 39.0 35.0 40.5 28.0 41.0 56-57 35.5403498460022 39.0 35.0 40.5 26.0 41.0 58-59 35.301523481283105 38.5 34.5 40.0 25.5 41.0 60-61 35.11316702631389 38.0 34.0 40.0 25.5 41.0 62-63 34.803518634872816 38.0 34.0 40.0 24.5 41.0 64-65 34.5303707579222 37.0 34.0 40.0 24.0 41.0 66-67 34.32775231750226 37.0 34.0 40.0 24.0 41.0 68-69 34.07973913467501 36.0 33.0 39.5 24.0 41.0 70-71 33.791640590402515 36.0 33.0 39.0 24.0 41.0 72-73 33.46951318183122 35.5 33.0 39.0 23.5 40.0 74-75 33.1101718202661 35.0 32.5 38.0 22.5 40.0 76-77 31.321872956585413 34.0 30.0 36.0 20.0 39.0 78-79 32.35365988048162 35.0 32.0 37.0 21.5 39.0 80-81 32.298335722047604 35.0 32.0 36.0 21.0 39.0 82-83 32.037323762732086 35.0 32.0 36.0 20.0 38.0 84-85 31.77562664377181 35.0 32.0 36.0 20.0 37.0 86-87 31.52945662469145 35.0 32.0 35.0 19.5 37.0 88-89 31.30967089615804 35.0 32.0 35.0 19.0 36.0 90-91 31.11905849849279 35.0 31.0 35.0 19.0 36.0 92-93 30.925434987019585 34.5 31.0 35.0 18.0 36.0 94-95 30.767345095587018 34.0 31.0 35.0 18.0 35.5 96-97 30.639705717407793 34.0 31.0 35.0 17.5 35.0 98-99 30.5197377194329 34.0 31.0 35.0 17.0 35.0 100 30.436389616326476 34.0 31.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.8148394892489996 1101 2 2.0601314378019673 1101 3 1.339398165870353 1101 4 1.1170265732325504 1101 5 0.663750925484166 1101 6 0.4661796285162012 1101 7 0.4118981886849795 1101 8 0.39483310155547 1101 9 0.39280648917092265 1101 10-11 0.3677431163247107 1101 12-13 0.6246591763058973 1101 14-15 0.6960345449480698 1101 16-17 0.49124621848713446 1101 18-19 0.45828859043336934 1101 20-21 0.43430056959439867 1101 22-23 0.4336516406187556 1101 24-25 0.4249832526857418 1101 26-27 0.4374668031457958 1101 28-29 0.4303541586521291 1101 30-31 0.4017216129145993 1101 32-33 0.38990283483800425 1101 34-35 0.39198209332088396 1101 36-37 0.37693237349797215 1101 38-39 0.43528358445365 1101 40-41 0.41583330542317753 1101 42-43 0.38997526540212135 1101 44-45 0.27798003301090546 1101 46-47 0.24024633655989192 1101 48-49 0.2510278695331465 1101 50-51 0.31756616402964255 1101 52-53 0.2991821745551917 1101 54-55 0.2839226441751421 1101 56-57 0.17974486183975813 1101 58-59 0.17005245892960374 1101 60-61 0.22991462411953734 1101 62-63 0.2902868101783156 1101 64-65 0.3123169094871727 1101 66-67 0.21649770861298379 1101 68-69 0.16775264499341347 1101 70-71 0.09466003551094815 1101 72-73 0.1308031567967589 1101 74-75 0.06847437484483265 1101 76-77 0.07306340411804157 1101 78-79 0.0698101006213534 1101 80-81 0.05894383898121447 1101 82-83 0.18561204212510063 1101 84-85 0.11110634182742274 1101 86-87 0.1235527061706776 1101 88-89 0.013020091639592835 1101 90-91 0.014065828471707675 1101 92-93 0.10629134583676247 1101 94-95 0.08132008443061878 1101 96-97 0.10067837741771157 1101 98-99 0.07601489427913677 1101 100 0.0757654458643664 1102 1 2.763897035786055 1102 2 2.0097761034095747 1102 3 1.2996157468292218 1102 4 1.0682685799887963 1102 5 0.6367662295704193 1102 6 0.45148819009524743 1102 7 0.336950413320686 1102 8 0.31839083904253584 1102 9 0.3232007136796682 1102 10-11 0.29808011412193025 1102 12-13 0.5481452713480692 1102 14-15 0.6400468875233045 1102 16-17 0.35723687311404717 1102 18-19 0.3797337839540944 1102 20-21 0.3713931033485025 1102 22-23 0.35799248329352196 1102 24-25 0.3390750510197549 1102 26-27 0.3194693324730764 1102 28-29 0.2685328079251619 1102 30-31 0.2635346602029571 1102 32-33 0.27966826337316064 1102 34-35 0.29510792499392835 1102 36-37 0.34076467600376503 1102 38-39 0.37030859694151985 1102 40-41 0.34719074165126784 1102 42-43 0.3106827590861272 1102 44-45 0.2662270802321913 1102 46-47 0.2796580536525113 1102 48-49 0.2332571410080817 1102 50-51 0.2503758278706343 1102 52-53 0.20912904818572287 1102 54-55 0.29254433691068726 1102 56-57 0.29867387392085476 1102 58-59 0.2623508976340787 1102 60-61 0.19353725521278164 1102 62-63 0.22710312996060367 1102 64-65 0.2039110809006459 1102 66-67 0.17027142529092032 1102 68-69 0.1944213775892294 1102 70-71 0.16071090118593645 1102 72-73 0.11469190044035571 1102 74-75 0.11325283316470802 1102 76-77 0.12278184974327644 1102 78-79 0.1985039736048293 1102 80-81 0.22468144353362618 1102 82-83 0.19790868777867132 1102 84-85 0.2045306983728139 1102 86-87 0.1807656278034635 1102 88-89 0.13749616151241284 1102 90-91 0.038415209192574906 1102 92-93 0.17208871594662156 1102 94-95 0.13098981222394812 1102 96-97 0.1554024876914788 1102 98-99 0.09896534002261959 1102 100 -0.004693994212988883 1103 1 2.7829186521566704 1103 2 1.9916151660183452 1103 3 1.2586805739066484 1103 4 1.0675892204215742 1103 5 0.6329863746409785 1103 6 0.4237595825776239 1103 7 0.3000032201868663 1103 8 0.2934245723815465 1103 9 0.30872999311799987 1103 10-11 0.29405480475288925 1103 12-13 0.5810140723404515 1103 14-15 0.6130793415815177 1103 16-17 0.37536597974990826 1103 18-19 0.26703548479076744 1103 20-21 0.25564981500662753 1103 22-23 0.29913218226686666 1103 24-25 0.3457878269240666 1103 26-27 0.29022560716166623 1103 28-29 0.31089866759551654 1103 30-31 0.3007341448361629 1103 32-33 0.30380435661533056 1103 34-35 0.32753580327575804 1103 36-37 0.28828449275575707 1103 38-39 0.3042025948765499 1103 40-41 0.3056561381311482 1103 42-43 0.26408276591487123 1103 44-45 0.29096247530141994 1103 46-47 0.1797969320303494 1103 48-49 0.16894073628420614 1103 50-51 0.20188230858911993 1103 52-53 0.1887344517589682 1103 54-55 0.2479779708949721 1103 56-57 0.2494386436786371 1103 58-59 0.19935239221420886 1103 60-61 0.22577263427943706 1103 62-63 0.24200437216511972 1103 64-65 0.20204822742655182 1103 66-67 0.12303777669558968 1103 68-69 0.14953652879422918 1103 70-71 0.045882248686503146 1103 72-73 0.021905869175661508 1103 74-75 0.06395271646231748 1103 76-77 -0.03852019051604927 1103 78-79 0.17405442207657984 1103 80-81 0.20111424109830267 1103 82-83 0.1819785418350648 1103 84-85 0.15903156677394392 1103 86-87 0.15282211011943758 1103 88-89 0.1584135057237681 1103 90-91 0.14388831075440223 1103 92-93 0.10964743252880638 1103 94-95 0.1073884958024891 1103 96-97 0.12256462258121914 1103 98-99 0.06027842365346814 1103 100 0.09432895106884231 1104 1 2.7143335304624934 1104 2 1.957875491041019 1104 3 1.245652050089877 1104 4 1.038314342537511 1104 5 0.5984512492685212 1104 6 0.38046977533012694 1104 7 0.30667095659478605 1104 8 0.3077522336918008 1104 9 0.3162869607855683 1104 10-11 0.2546509018573744 1104 12-13 0.493846323160561 1104 14-15 0.515412215566549 1104 16-17 0.32679394231848136 1104 18-19 0.2714459562889999 1104 20-21 0.29190897152726336 1104 22-23 0.3179845899635154 1104 24-25 0.2721672471966414 1104 26-27 0.26633357674140967 1104 28-29 0.21245648294580377 1104 30-31 0.22298129063334216 1104 32-33 0.24809355668609356 1104 34-35 0.26506690408660916 1104 36-37 0.21804686056648137 1104 38-39 0.2676537328143027 1104 40-41 0.24553142839465636 1104 42-43 0.2620230931197014 1104 44-45 0.20386539471096654 1104 46-47 0.2528687456918277 1104 48-49 0.23077887691950139 1104 50-51 0.19734177736269487 1104 52-53 0.2499807793549138 1104 54-55 0.280757848676771 1104 56-57 0.23514858831239138 1104 58-59 0.12574032961033765 1104 60-61 0.12292953701312115 1104 62-63 0.18829161932320204 1104 64-65 0.1894055627575213 1104 66-67 0.1654001234019873 1104 68-69 0.14549237908888557 1104 70-71 0.1685547142872892 1104 72-73 0.11383561001705544 1104 74-75 0.09298790763376275 1104 76-77 0.07380105310861396 1104 78-79 0.1824427813781 1104 80-81 0.1717592458127868 1104 82-83 0.19669794282022934 1104 84-85 0.1613809899972729 1104 86-87 0.11983505245797232 1104 88-89 0.1779983372572964 1104 90-91 0.21566077537018202 1104 92-93 0.24592504811247196 1104 94-95 0.2501993345403868 1104 96-97 0.23096653699359493 1104 98-99 0.2139653302006046 1104 100 0.21649703987981894 1105 1 2.66409965976575 1105 2 1.9256451791188347 1105 3 1.2046612958487835 1105 4 1.0300091686766564 1105 5 0.5742043349259589 1105 6 0.3697721372090257 1105 7 0.2503526657796158 1105 8 0.24483660619759462 1105 9 0.26523405763479957 1105 10-11 0.22428676024398442 1105 12-13 0.4341828248694881 1105 14-15 0.5027797763613719 1105 16-17 0.2915647049884029 1105 18-19 0.22251051826819435 1105 20-21 0.24000989254556515 1105 22-23 0.22179820138777018 1105 24-25 0.21245875443523232 1105 26-27 0.2481396451630573 1105 28-29 0.2025076921225306 1105 30-31 0.19313254287516202 1105 32-33 0.21822716184697555 1105 34-35 0.2592113228424182 1105 36-37 0.27159556030691334 1105 38-39 0.2853211615712681 1105 40-41 0.2389878295786616 1105 42-43 0.24467956334542862 1105 44-45 0.24137931941389468 1105 46-47 0.1801890412600855 1105 48-49 0.17769303781643941 1105 50-51 0.18997008696634765 1105 52-53 0.1960062083856613 1105 54-55 0.2104581005962629 1105 56-57 0.23144433827258126 1105 58-59 0.2076534356169546 1105 60-61 0.17859442923414548 1105 62-63 0.2345564171874699 1105 64-65 0.2210474297784586 1105 66-67 0.1835924471675625 1105 68-69 0.16856944691753029 1105 70-71 0.1490901588792397 1105 72-73 0.14422437885323092 1105 74-75 0.16503815579504533 1105 76-77 0.1523091905502021 1105 78-79 0.24640602761367347 1105 80-81 0.2555985843786246 1105 82-83 0.22713572322994224 1105 84-85 0.20297771478453797 1105 86-87 0.20585901026717934 1105 88-89 0.221243395581606 1105 90-91 0.23921114247561093 1105 92-93 0.2576180583461003 1105 94-95 0.2505517369832866 1105 96-97 0.2577044536321651 1105 98-99 0.22391420845780985 1105 100 0.2466016193084961 1106 1 2.598897278915363 1106 2 1.8662704412778233 1106 3 1.146451262504936 1106 4 0.9838471422845387 1106 5 0.5657833956746927 1106 6 0.3774713767483817 1106 7 0.27473511905124326 1106 8 0.25034963579513203 1106 9 0.28470231022235737 1106 10-11 0.1928928318245937 1106 12-13 0.4826472079183972 1106 14-15 0.463190830945706 1106 16-17 0.3090247800234067 1106 18-19 0.25461655669893446 1106 20-21 0.24624564040326646 1106 22-23 0.22124559878361794 1106 24-25 0.2350055224229095 1106 26-27 0.20492212702787782 1106 28-29 0.22371526316759827 1106 30-31 0.266898153516145 1106 32-33 0.2672344325622049 1106 34-35 0.23486884885655002 1106 36-37 0.31016795149383114 1106 38-39 0.25911668075598726 1106 40-41 0.3159581926224533 1106 42-43 0.3268697404132368 1106 44-45 0.28129337355949247 1106 46-47 0.19331361353723509 1106 48-49 0.21426222076458146 1106 50-51 0.2677942638277955 1106 52-53 0.26031471541613627 1106 54-55 0.13331694141646722 1106 56-57 0.1700003335111191 1106 58-59 0.17141715615234432 1106 60-61 0.18765018574183756 1106 62-63 0.19266834232760033 1106 64-65 0.14579706281785576 1106 66-67 0.058369331078161224 1106 68-69 0.14183847185061182 1106 70-71 0.08128641158375416 1106 72-73 0.10040697887036742 1106 74-75 0.12263718832043935 1106 76-77 0.033408300829481874 1106 78-79 0.16041359869928584 1106 80-81 0.19547810633606844 1106 82-83 0.16048931133997968 1106 84-85 0.12581313406234784 1106 86-87 0.1320626270900327 1106 88-89 0.17897665314855615 1106 90-91 0.19151780588306977 1106 92-93 0.1951751357324305 1106 94-95 0.2158753109593583 1106 96-97 0.217593831256508 1106 98-99 0.21840984339760539 1106 100 0.2809950554187033 1107 1 0.6482522941316446 1107 2 0.9602118050902568 1107 3 0.7461774468981552 1107 4 0.7865506608455703 1107 5 0.43618269798015774 1107 6 0.2968252108002929 1107 7 0.19738518971659147 1107 8 0.22091519690373929 1107 9 0.2608801846293929 1107 10-11 0.1702058109411979 1107 12-13 0.4536654217651872 1107 14-15 0.3993188875638296 1107 16-17 0.25396947553957006 1107 18-19 0.22689718720570795 1107 20-21 0.24429513193896923 1107 22-23 0.21851180703149709 1107 24-25 0.23529560729318888 1107 26-27 0.16966527650507857 1107 28-29 0.18981382452631834 1107 30-31 0.1521391056410195 1107 32-33 0.2285998497496493 1107 34-35 0.1334846794325415 1107 36-37 0.18648746147187722 1107 38-39 0.25068625052909965 1107 40-41 0.17241791857546218 1107 42-43 0.252876011827901 1107 44-45 0.216573671249229 1107 46-47 0.22064024379803016 1107 48-49 0.25718852730076947 1107 50-51 0.1846152765744904 1107 52-53 0.20906269293750768 1107 54-55 0.17380475741902757 1107 56-57 0.19594135898420717 1107 58-59 0.1274209275605287 1107 60-61 0.13777794811944233 1107 62-63 0.24896574018246298 1107 64-65 0.17876564488353353 1107 66-67 0.1720492291979312 1107 68-69 0.16330531858544362 1107 70-71 0.12518193832757163 1107 72-73 0.0773383284478868 1107 74-75 -0.022462999505343362 1107 76-77 -0.04204623706671029 1107 78-79 0.18443598591757393 1107 80-81 0.18348325829723677 1107 82-83 0.132146255885786 1107 84-85 0.1343913391230771 1107 86-87 0.12928709948927164 1107 88-89 0.21894199459407915 1107 90-91 0.23802210850420025 1107 92-93 0.20554271566832583 1107 94-95 0.20846960709452134 1107 96-97 0.27382253996046657 1107 98-99 0.2644109130744674 1107 100 0.27097707171109064 1108 1 0.8123253157575583 1108 2 1.0441151927195627 1108 3 0.7446871037559681 1108 4 0.7828081050716094 1108 5 0.38507781117032636 1108 6 0.2257572623144668 1108 7 0.15431716666376616 1108 8 0.15619932250553603 1108 9 0.1379584723645948 1108 10-11 0.06493416128564888 1108 12-13 0.36017083957089113 1108 14-15 0.3227501586072705 1108 16-17 0.11946665661883316 1108 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-0.9821171776036799 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 23.0 7 531.0 8 644.0 9 320.0 10 179.0 11 174.0 12 404.0 13 1692.0 14 7222.0 15 20089.0 16 37135.0 17 53687.0 18 71639.0 19 96106.0 20 128036.0 21 174341.0 22 248859.0 23 373046.0 24 558219.0 25 680338.0 26 651334.0 27 599948.0 28 608507.0 29 679850.0 30 791040.0 31 952271.0 32 1164559.0 33 1460860.0 34 1932352.0 35 2764875.0 36 4177760.0 37 6800523.0 38 7630590.0 39 1968102.0 40 1928.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.958007372581772 35.11054142525761 28.382695311954166 18.548755890206454 2 19.960065445298635 33.78956670566427 28.57001225648596 17.680355592551138 3 24.91594076804687 27.9040763794215 27.766140797304445 19.41384205522718 4 19.372165419581922 27.992601360947937 27.53372452567661 25.101508693793527 5 19.57072548307407 33.676035375047675 27.337560909615544 19.41567823226271 6 25.630228070221527 27.578821868972426 27.571849592964874 19.219100467841162 7 19.524771976982077 27.604718894143325 33.60321767506324 19.267291453811357 8 25.24569910896045 27.65351039084212 27.741618017839382 19.359172482358048 9 25.06236145127622 27.439803635301402 28.115886329439665 19.381948583982712 10-11 19.011462335144287 31.00235229868434 30.74311499292538 19.243070373246 12-13 19.043299648263257 27.99688932033843 30.54450240218217 22.415308629216142 14-15 18.99067686884352 34.22967883964467 27.389571490268132 19.39007280124368 16-17 22.184624530858088 27.889424524067696 27.42796655408435 22.497984390989863 18-19 19.248450429701514 31.03531398612872 27.442921671776833 22.273313912392933 20-21 22.240030316553167 31.060861675731537 27.380722618233705 19.318385389481588 22-23 22.195047665471723 28.093393112450165 30.436942765504334 19.27461645657378 24-25 19.133952094263712 30.99119902429548 27.337150133893186 22.537698747547626 26-27 19.194811469500213 27.934104311502956 33.530328367641495 19.34075585135534 28-29 19.16926290144381 28.032999915596474 33.48498973480217 19.31274744815755 30-31 19.272955477793417 27.999018740262336 33.45597107218116 19.272054709763086 32-33 19.27064824392729 28.112716072190715 33.28767259336748 19.32896309051451 34-35 19.289253401467434 28.14312006839586 33.27812484057956 19.289501689557145 36-37 19.39620205257454 28.13532786427811 33.214206536368735 19.254263546778617 38-39 19.37785882365882 28.18692854833509 33.1502770989911 19.284935529014994 40-41 19.438321687608763 28.224231623970486 33.132922936122824 19.204523752297927 42-43 19.401492046812095 28.234891778088805 33.136151314210124 19.227464860888972 44-45 19.456610422575547 28.29546596252806 33.02503499136187 19.22288862353452 46-47 19.543761425293425 28.357490953451293 32.94661691789056 19.152130703364723 48-49 19.5513544659602 28.43553730300355 32.821297569649026 19.191810661387226 50-51 19.579159406282074 28.474815927160453 32.63925384922625 19.306770817331227 52-53 19.696313590589057 28.621867199581896 32.34353108119689 19.338288128632154 54-55 19.960506019246367 28.88312689255833 31.62897901711654 19.527388071078768 56-57 20.35992331019602 29.459246520668202 30.476723787677972 19.704106381457805 58-59 20.552088687986643 29.85832465719711 29.760710812401953 19.8288758424143 60-61 20.608331866172392 30.18473229235436 29.30860106793911 19.898334773534142 62-63 20.63332747732404 30.361020446126258 29.113759774119664 19.89189230243004 64-65 20.72123073461767 30.586510624217873 28.753447557475344 19.93881108368912 66-67 20.6799217180023 30.58089003335781 28.747689428397656 19.99149882024223 68-69 20.662290519124955 30.42664406892216 28.890867474767013 20.020197937185873 70-71 20.559374623656485 30.310365615261354 29.041853917490744 20.08840584359141 72-73 20.47595984463446 30.209576643866704 29.062925398798246 20.251538112700594 74-75 20.401246367979283 30.25566007398498 28.94426232972832 20.39883122830741 76-77 20.45770518255842 30.288514132957552 28.778733448935473 20.47504723554856 78-79 20.474891839976312 30.520796601575807 28.39510375968457 20.60920779876331 80-81 20.544756565418933 30.682447979772064 28.043675060271067 20.72912039453793 82-83 20.600834022006758 30.89222902841748 27.58852172252323 20.91841522705253 84-85 20.54718894700314 31.081575434714015 27.303860650734535 21.067374967548307 86-87 20.526486100406547 31.154706384229176 27.043168412757584 21.275639102606696 88-89 20.608302980777303 31.22503183255222 26.792939941682377 21.3737252449881 90-91 20.679335531152105 31.18108666850462 26.668094049196423 21.471483751146852 92-93 20.686392863774493 31.10307471795293 26.669016117596193 21.541516300676385 94-95 20.7478901879239 31.094223680032545 26.577897336061124 21.579988795982434 96-97 20.732516493672335 31.110810808899007 26.474323821617656 21.682348875811 98-99 20.687602990160407 31.073891805952996 26.40389396204107 21.834611241845522 100 20.62933351133087 31.05805399936825 26.32911862376337 21.983493865537508 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 126293.0 1 76648.0 2 21009.5 3 14513.5 4 14057.5 5 14614.5 6 15252.5 7 14976.5 8 14715.5 9 15064.5 10 16169.5 11 22912.0 12 158739.5 13 182657.0 14 74119.0 15 77703.0 16 90545.0 17 104219.5 18 118676.5 19 134173.0 20 152741.5 21 181466.0 22 219587.0 23 266200.0 24 324182.0 25 382962.5 26 440381.5 27 511056.5 28 604336.5 29 704337.5 30 787829.0 31 863942.5 32 924388.5 33 939588.5 34 946311.0 35 978905.0 36 1031640.0 37 1089681.5 38 1132591.0 39 1131052.5 40 1105293.0 41 1112797.5 42 1165540.5 43 1175382.5 44 1156171.0 45 1170268.0 46 1180668.0 47 1185151.5 48 1137522.5 49 1059559.5 50 1024588.0 51 993876.5 52 953664.0 53 897382.0 54 807250.5 55 708972.0 56 607235.5 57 510356.0 58 431464.0 59 353715.5 60 278023.0 61 233133.0 62 187036.0 63 119610.5 64 67642.5 65 41540.5 66 27808.0 67 21271.5 68 15965.5 69 11491.5 70 8521.0 71 6448.5 72 5435.0 73 4731.5 74 4175.0 75 4026.0 76 3018.5 77 1429.0 78 581.5 79 369.5 80 282.5 81 203.0 82 135.5 83 86.5 84 66.5 85 55.5 86 40.5 87 29.0 88 25.0 89 14.5 90 12.5 91 13.0 92 6.0 93 2.5 94 0.0 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20655259407209875 2 3.4644849726953835E-5 3 0.0 4 5.7741416211589724E-6 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.3834795110214361 14-15 0.0 16-17 2.5983637295215374E-5 18-19 0.0 20-21 0.0 22-23 1.0104747837028203E-5 24-25 1.6600657160832047E-4 26-27 4.4749597563982035E-5 28-29 1.0104747837028203E-5 30-31 2.150867753881717E-4 32-33 5.947365869793742E-4 34-35 0.0 36-37 0.0 38-39 3.608838513224358E-5 40-41 2.309656648463589E-5 42-43 1.905466734982461E-4 44-45 1.8188546106650763E-4 46-47 6.264943658957485E-4 48-49 0.002686419389244212 50-51 0.018266497018536408 52-53 0.04631438994331612 54-55 0.05922970121444345 56-57 0.05943468324199459 58-59 0.05970318082737849 60-61 0.0524523024866081 62-63 0.02580030829874358 64-65 0.010931893624259224 66-67 0.009141909721699943 68-69 0.007096420052404378 70-71 0.008470665758240212 72-73 0.0101191831910811 74-75 0.004345041569922127 76-77 0.0049715359358178755 78-79 0.019913570915972005 80-81 0.03748428386915876 82-83 0.046984190371370554 84-85 0.04761068473726631 86-87 0.04258285092064213 88-89 0.02516226564960551 90-91 0.007773438157485268 92-93 0.0020830215898330995 94-95 0.006598400337579417 96-97 0.0049181251258221545 98-99 0.0010941998372096253 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.4637183E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.925405575040916 #Duplication Level Percentage of deduplicated Percentage of total 1 67.354832505536 14.76782020122826 2 13.654603295145954 5.987654304247302 3 5.451353171343494 3.585693876434749 4 2.8890699024427806 2.5337611738280743 5 1.8036307749881135 1.9772668124619874 6 1.1915660863795348 1.5675341828001323 7 0.8683424863869509 1.332713283445134 8 0.6942089904529426 1.2176650935616378 9 0.5593102802692252 1.103679426347331 >10 4.497576132478658 19.705810670855257 >50 0.5768862479518496 8.91067295398264 >100 0.39009331877672054 15.657721961812776 >500 0.046733364220088044 7.785996786701683 >1k 0.021551365241041013 7.582943683419037 >5k 1.4793679202922426E-4 0.2001078850219278 >10k+ 9.414159488444285E-5 6.0829577038521245 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1771291 5.113842543142149 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 150783 0.43532119803160674 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 48981 0.1414116153729938 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 35280 0.10185585819724428 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.11653661326904097 0.0 2.8870708105794862E-6 2.8870708105794862E-6 0.0 2 0.11675314357983442 0.0 2.8870708105794862E-6 2.8870708105794862E-6 0.0 3 0.11680222378361427 0.0 2.8870708105794862E-6 2.8870708105794862E-6 0.0 4 0.11683975570415181 0.0 2.8870708105794862E-6 2.8870708105794862E-6 0.0 5 0.11684264277496238 0.0 2.8870708105794862E-6 2.8870708105794862E-6 0.0 6 0.11697544803224905 2.8870708105794862E-6 2.8870708105794862E-6 5.7741416211589724E-6 0.0 7 0.11697833510305962 2.8870708105794862E-6 2.8870708105794862E-6 8.661212431738459E-6 0.0 8 0.1169812221738702 2.8870708105794862E-6 2.8870708105794862E-6 8.661212431738459E-6 0.0 9 0.11698410924468078 2.8870708105794862E-6 2.8870708105794862E-6 8.661212431738459E-6 0.0 10-11 0.1169942139925178 2.8870708105794862E-6 2.8870708105794862E-6 1.0104747837028201E-5 0.0 12-13 0.11704040712548708 2.8870708105794862E-6 2.8870708105794862E-6 1.1548283242317945E-5 0.0 14-15 0.11709814854169867 2.8870708105794862E-6 2.8870708105794862E-6 1.1548283242317945E-5 2.8870708105794862E-6 16-17 0.11712990632061504 2.8870708105794862E-6 2.8870708105794862E-6 1.1548283242317945E-5 2.8870708105794862E-6 18-19 0.11720641369709539 2.8870708105794862E-6 2.8870708105794862E-6 1.1548283242317945E-5 2.8870708105794862E-6 20-21 0.11723817147601179 2.8870708105794862E-6 2.8870708105794862E-6 1.1548283242317945E-5 2.8870708105794862E-6 22-23 0.11734354956059792 2.8870708105794862E-6 2.8870708105794862E-6 1.1548283242317945E-5 2.8870708105794862E-6 24-25 0.11793251200595614 2.8870708105794862E-6 2.8870708105794862E-6 1.1548283242317945E-5 2.8870708105794862E-6 26-27 0.12037353037630111 2.8870708105794862E-6 2.8870708105794862E-6 1.1548283242317945E-5 2.8870708105794862E-6 28-29 0.1278755838775919 2.8870708105794862E-6 2.8870708105794862E-6 1.2991818647607689E-5 2.8870708105794862E-6 30-31 0.14632829696341068 2.8870708105794862E-6 2.8870708105794862E-6 1.4435354052897431E-5 2.8870708105794862E-6 32-33 0.18331889172396035 2.8870708105794862E-6 2.8870708105794862E-6 1.7322424863476917E-5 2.8870708105794862E-6 34-35 0.2460361744775838 2.8870708105794862E-6 2.8870708105794862E-6 1.7322424863476917E-5 2.8870708105794862E-6 36-37 0.3415217109312845 2.8870708105794862E-6 2.8870708105794862E-6 1.7322424863476917E-5 2.8870708105794862E-6 38-39 0.47614437929320064 2.8870708105794862E-6 2.8870708105794862E-6 1.7322424863476917E-5 2.8870708105794862E-6 40-41 0.6536328315151957 2.8870708105794862E-6 2.8870708105794862E-6 1.7322424863476917E-5 2.8870708105794862E-6 42-43 0.8770444755856733 2.8870708105794862E-6 2.8870708105794862E-6 1.7322424863476917E-5 2.8870708105794862E-6 44-45 1.1489747881633445 2.8870708105794862E-6 2.8870708105794862E-6 1.7322424863476917E-5 2.8870708105794862E-6 46-47 1.4721996878325814 2.8870708105794862E-6 2.8870708105794862E-6 1.7322424863476917E-5 2.8870708105794862E-6 48-49 1.8474828048227825 2.8870708105794862E-6 2.8870708105794862E-6 1.876596026876666E-5 2.8870708105794862E-6 50-51 2.2717970453890546 2.8870708105794862E-6 2.8870708105794862E-6 2.0209495674056405E-5 2.8870708105794862E-6 52-53 2.740461024212044 2.8870708105794862E-6 2.8870708105794862E-6 2.0209495674056405E-5 2.8870708105794862E-6 54-55 3.254179186569531 2.8870708105794862E-6 2.8870708105794862E-6 2.4540101889925634E-5 2.8870708105794862E-6 56-57 3.818776197821861 4.330606215869229E-6 2.8870708105794862E-6 2.5983637295215378E-5 2.8870708105794862E-6 58-59 4.425279041889752 8.661212431738459E-6 2.8870708105794862E-6 2.742717270050512E-5 2.8870708105794862E-6 60-61 5.075357889237124 1.4435354052897431E-5 2.8870708105794862E-6 2.8870708105794862E-5 2.8870708105794862E-6 62-63 5.755798616763956 1.4435354052897431E-5 2.8870708105794862E-6 2.8870708105794862E-5 2.8870708105794862E-6 64-65 6.465430517256556 1.4435354052897431E-5 2.8870708105794862E-6 2.8870708105794862E-5 2.8870708105794862E-6 66-67 7.201993014270243 1.7322424863476917E-5 2.8870708105794862E-6 3.0314243511084603E-5 2.8870708105794862E-6 68-69 7.96534031072908 1.7322424863476917E-5 2.8870708105794862E-6 3.175777891637435E-5 2.8870708105794862E-6 70-71 8.753864308191575 1.7322424863476917E-5 2.8870708105794862E-6 3.175777891637435E-5 2.8870708105794862E-6 72-73 9.562913935581886 1.7322424863476917E-5 2.8870708105794862E-6 3.320131432166409E-5 4.330606215869229E-6 74-75 10.388669598217614 1.7322424863476917E-5 2.8870708105794862E-6 3.4644849726953835E-5 5.7741416211589724E-6 76-77 11.230384988294228 1.7322424863476917E-5 2.8870708105794862E-6 3.4644849726953835E-5 5.7741416211589724E-6 78-79 12.08952529424809 1.7322424863476917E-5 2.8870708105794862E-6 3.753192053753332E-5 5.7741416211589724E-6 80-81 12.96252642716355 1.7322424863476917E-5 2.8870708105794862E-6 4.330606215869229E-5 5.7741416211589724E-6 82-83 13.845365253866055 1.876596026876666E-5 2.8870708105794862E-6 4.4749597563982035E-5 5.7741416211589724E-6 84-85 14.735925551451455 2.0209495674056405E-5 2.8870708105794862E-6 4.908020377985127E-5 5.7741416211589724E-6 86-87 15.631588746694558 2.309656648463589E-5 2.8870708105794862E-6 4.908020377985127E-5 5.7741416211589724E-6 88 16.30817090408305 2.309656648463589E-5 2.8870708105794862E-6 4.908020377985127E-5 5.7741416211589724E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 228205 0.0 86.87286 8 AGTGGTA 231070 0.0 86.698586 5 GTGGTAT 233270 0.0 85.675575 6 TGGTATC 231480 0.0 85.639694 7 GTATCAA 230805 0.0 85.19915 9 GCAGTGG 235925 0.0 85.19314 3 AAGCAGT 236035 0.0 81.71597 1 CAGTGGT 251545 0.0 79.748 4 AGCAGTG 252405 0.0 79.548965 2 CAACGCA 223010 0.0 45.789444 12-13 ATCAACG 223340 0.0 45.629612 10-11 ACGCAGA 230045 0.0 44.938774 14-15 AGAGTAC 233970 0.0 44.018555 18-19 TCAACGC 223040 0.0 43.9946 12-13 AACGCAG 226735 0.0 43.33408 14-15 TATCAAC 227735 0.0 43.111504 10-11 CGCAGAG 230425 0.0 42.613697 16-17 GAGTACT 230975 0.0 42.042767 20-21 AGTACTT 246870 0.0 41.713673 20-21 CAGAGTA 239860 0.0 40.701717 18-19 >>END_MODULE