FastQCFastQC Report
Thu 26 May 2016
SRR1660683_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660683_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51392450
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35880396.981646136737984No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2308260.44914379446786445No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880620.1713520176601816No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT871950.16966499943085026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4514850.086.982068
AGTGGTA4546950.086.500035
AAGCAGT4664550.086.288581
TGGTATC4536050.086.096457
GCAGTGG4673350.085.7253953
GTATCAA4555350.085.7172559
GTGGTAT4585200.085.5154656
AGCAGTG4809700.083.678662
CAGTGGT4880600.081.0502244
ATCAACG4478600.045.6751510-11
CAACGCA4487100.045.60183312-13
ACGCAGA4539700.045.2157114-15
AGAGTAC4597900.043.94183718-19
TCAACGC4495350.043.71251312-13
AACGCAG4506200.043.3912214-15
TATCAAC4513300.043.1760210-11
CGCAGAG4552750.042.74068516-17
AGTACTT4771850.042.3659220-21
GCAGAGT4800600.042.15374416-17
GAGTACT4531250.041.99041420-21
Produced by FastQC (version 0.11.5)