##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660683_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51392450 Sequences flagged as poor quality 0 Sequence length 100 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.353877738850745 31.0 31.0 34.0 28.0 34.0 2 31.498093805607635 31.0 31.0 34.0 28.0 34.0 3 31.55162900387119 33.0 31.0 34.0 28.0 34.0 4 35.094789156773025 37.0 35.0 37.0 32.0 37.0 5 35.057656289980336 37.0 35.0 37.0 32.0 37.0 6 35.1011762817301 37.0 35.0 37.0 32.0 37.0 7 34.99791852694316 37.0 35.0 37.0 32.0 37.0 8 34.96636729714189 37.0 35.0 37.0 32.0 37.0 9 36.58013393017846 39.0 37.0 39.0 32.0 39.0 10-11 36.48098147685117 39.0 37.0 39.0 32.0 39.0 12-13 36.55572769930214 39.0 37.0 39.0 32.0 39.0 14-15 37.83853059155577 40.0 38.0 41.0 32.0 41.0 16-17 37.72774666317718 40.0 37.0 41.0 32.0 41.0 18-19 37.66418599424624 40.0 37.0 41.0 32.0 41.0 20-21 37.55322165026186 40.0 37.0 41.0 31.5 41.0 22-23 37.34594107694807 40.0 36.5 41.0 31.5 41.0 24-25 37.393019071867556 40.0 37.0 41.0 31.0 41.0 26-27 37.42256137817909 40.0 37.0 41.0 31.0 41.0 28-29 37.320610731731996 40.0 37.0 41.0 31.0 41.0 30-31 37.14706621303324 40.0 36.0 41.0 30.5 41.0 32-33 37.03876188039294 40.0 36.0 41.0 30.0 41.0 34-35 36.887645588019254 40.0 36.0 41.0 30.0 41.0 36-37 36.64319572232887 39.0 36.0 40.5 30.0 41.0 38-39 36.15156694222595 38.0 35.0 40.0 29.5 41.0 40-41 36.2642448355741 39.0 35.0 40.0 30.0 41.0 42-43 36.13807890069456 38.5 35.0 40.0 29.0 41.0 44-45 35.98830092747086 38.0 35.0 40.0 28.0 41.0 46-47 35.94714784953821 38.0 35.0 40.0 28.0 41.0 48-49 35.998005825758455 39.0 35.0 40.0 28.0 41.0 50-51 34.99781394932524 38.0 34.0 39.5 25.5 40.5 52-53 34.936139053499105 38.0 33.5 39.5 25.5 40.5 54-55 34.9800659435384 38.5 34.0 40.0 22.5 41.0 56-57 34.556471874759815 38.5 33.5 41.0 20.0 41.0 58-59 34.34098408423806 38.0 33.0 41.0 18.5 41.0 60-61 34.189380833176855 38.0 33.0 40.0 18.0 41.0 62-63 33.964423606969504 38.0 33.0 40.0 18.0 41.0 64-65 33.72827264510643 37.0 33.0 40.0 18.0 41.0 66-67 33.47794846324703 37.0 32.0 40.0 18.0 41.0 68-69 33.210954147934174 36.0 32.0 39.5 18.0 41.0 70-71 32.9778196019065 36.0 32.0 39.0 18.0 41.0 72-73 32.68121676433017 35.0 31.5 39.0 18.0 41.0 74-75 32.31547004472447 35.0 31.0 38.5 17.5 40.0 76-77 31.927191309229272 35.0 31.0 37.0 16.0 39.5 78-79 31.56709793948333 35.0 31.0 37.0 15.0 39.0 80-81 31.20013586626051 35.0 30.0 36.0 13.5 39.0 82-83 30.86506635702326 35.0 30.0 36.0 11.5 37.5 84-85 30.542399253197697 35.0 30.0 35.5 10.0 37.0 86-87 30.257250325680133 34.0 29.0 35.0 10.0 37.0 88-89 30.024936094698734 34.0 29.0 35.0 10.0 36.0 90-91 29.80647284766537 34.0 29.0 35.0 10.0 36.0 92-93 29.64580353729001 34.0 29.0 35.0 10.0 36.0 94-95 29.50750131585476 34.0 29.0 35.0 10.0 35.5 96-97 29.331781176028777 34.0 29.0 35.0 10.0 35.0 98-99 29.19551872891835 34.0 28.0 35.0 10.0 35.0 100 29.091238090419896 34.0 27.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5828212421763936 1101 2 0.4907696962122934 1101 3 0.530404167326548 1101 4 0.44809079084468095 1101 5 0.5142610716885017 1101 6 0.5443876788669115 1101 7 0.5527803148439148 1101 8 0.6196933566163736 1101 9 0.6581391181102418 1101 10-11 0.619987573652999 1101 12-13 0.5979028286949415 1101 14-15 0.6537590222107994 1101 16-17 0.652485937280467 1101 18-19 0.708221042250841 1101 20-21 0.7079091554375765 1101 22-23 0.6652859712154395 1101 24-25 0.6421330616256284 1101 26-27 0.6178226463390715 1101 28-29 0.6425808734851728 1101 30-31 0.5302610114100119 1101 32-33 0.5622151371806652 1101 34-35 0.5188270609460943 1101 36-37 0.5752236465193405 1101 38-39 0.5433409136373513 1101 40-41 0.5488568401716805 1101 42-43 0.4939977574508134 1101 44-45 0.48849629831317287 1101 46-47 0.5113627312197977 1101 48-49 0.545090576317051 1101 50-51 0.5278421206530055 1101 52-53 0.5379626038297545 1101 54-55 0.5741647308049167 1101 56-57 0.5581133492029124 1101 58-59 0.5328460539249136 1101 60-61 0.46880111972516403 1101 62-63 0.499841255661579 1101 64-65 0.39494080807063625 1101 66-67 0.4487072015237388 1101 68-69 0.33835738288492223 1101 70-71 0.3403887731039177 1101 72-73 0.30222707612938393 1101 74-75 0.2809561788912305 1101 76-77 0.3123977125239712 1101 78-79 0.2278908696543347 1101 80-81 0.23159160464133777 1101 82-83 0.19652588102859525 1101 84-85 0.16076481595324665 1101 86-87 0.17064914377832352 1101 88-89 0.11604106697256711 1101 90-91 0.12417709729341908 1101 92-93 0.060336378104580035 1101 94-95 -0.029479661445353855 1101 96-97 -0.053144081793096376 1101 98-99 -0.023397935238413936 1101 100 -0.0031148285874422754 1102 1 0.49172735758452646 1102 2 0.4925773015427062 1102 3 0.4975558641853759 1102 4 0.4189183526869371 1102 5 0.3697790171272004 1102 6 0.41756769277304073 1102 7 0.5107884940949745 1102 8 0.6247076759235668 1102 9 0.5995040939541454 1102 10-11 0.5602961445426757 1102 12-13 0.5142826335615851 1102 14-15 0.6304214388663993 1102 16-17 0.6688305921972386 1102 18-19 0.5671916021426995 1102 20-21 0.546085898081742 1102 22-23 0.568780161299955 1102 24-25 0.5301879433838224 1102 26-27 0.5276137644922372 1102 28-29 0.5370696408181246 1102 30-31 0.5143815436981569 1102 32-33 0.48031114178334633 1102 34-35 0.4439008148994077 1102 36-37 0.5023049041581444 1102 38-39 0.45007802082901094 1102 40-41 0.45010297885786343 1102 42-43 0.4638787308135832 1102 44-45 0.42763421337264873 1102 46-47 0.46950592604034824 1102 48-49 0.5070376530520875 1102 50-51 0.47199308635843096 1102 52-53 0.4797749509762568 1102 54-55 0.5600430228009827 1102 56-57 0.5531239374145116 1102 58-59 0.4905702076147449 1102 60-61 0.421197377018089 1102 62-63 0.4528580355141898 1102 64-65 0.3244162223505569 1102 66-67 0.27110284113238237 1102 68-69 0.3262430366525848 1102 70-71 0.3824160947541415 1102 72-73 0.3330815033964427 1102 74-75 0.33282621034731363 1102 76-77 0.2535626219998086 1102 78-79 0.2106523468602184 1102 80-81 0.23969719464640704 1102 82-83 0.22770533596037978 1102 84-85 0.18494081880846025 1102 86-87 0.17581223451511363 1102 88-89 0.18210884821721507 1102 90-91 0.14666165215234983 1102 92-93 0.10544617301493275 1102 94-95 0.08018012119385176 1102 96-97 0.09793527341008712 1102 98-99 0.09220036460121861 1102 100 0.06879145645183016 1103 1 0.4805898212848625 1103 2 0.4751034751144019 1103 3 0.3898625826204629 1103 4 0.38563289955467894 1103 5 0.4553119341637597 1103 6 0.44354228518980676 1103 7 0.4313375970684987 1103 8 0.5358114847735536 1103 9 0.5797539354178767 1103 10-11 0.5409713548509885 1103 12-13 0.5711957326489809 1103 14-15 0.572792676619251 1103 16-17 0.6348409087422056 1103 18-19 0.6350105696940886 1103 20-21 0.6432920509325442 1103 22-23 0.6110698885529189 1103 24-25 0.5505414224968348 1103 26-27 0.5157215084521098 1103 28-29 0.49114253565623045 1103 30-31 0.5145111522337871 1103 32-33 0.5003264555920168 1103 34-35 0.5097213174761777 1103 36-37 0.4813828239018889 1103 38-39 0.46502885356871104 1103 40-41 0.4724791278305034 1103 42-43 0.46099341338226196 1103 44-45 0.4209198885430041 1103 46-47 0.42541508610705847 1103 48-49 0.42439497446687113 1103 50-51 0.38042941438813216 1103 52-53 0.4000924861678996 1103 54-55 0.5233690403959272 1103 56-57 0.5304796296209986 1103 58-59 0.46431514086069825 1103 60-61 0.3647905279620858 1103 62-63 0.3895106593995976 1103 64-65 0.3488648501574474 1103 66-67 0.3093738269070272 1103 68-69 0.288219101836134 1103 70-71 0.32507321207037876 1103 72-73 0.2796253877803494 1103 74-75 0.2980199189579835 1103 76-77 0.2830857995365399 1103 78-79 0.22269516408954004 1103 80-81 0.16704087138169754 1103 82-83 0.1343312548240405 1103 84-85 0.10259923337895671 1103 86-87 0.11986728322031581 1103 88-89 0.0921554470952195 1103 90-91 0.08999915254536361 1103 92-93 0.04384767125396394 1103 94-95 0.0928098790166878 1103 96-97 0.11659223919265216 1103 98-99 0.1272816836868209 1103 100 0.09411712718802079 1104 1 0.4265283365698487 1104 2 0.47546624872327925 1104 3 0.39120823032545715 1104 4 0.352075370113738 1104 5 0.4348051416934311 1104 6 0.41936871209092885 1104 7 0.4835047759993998 1104 8 0.532488449919363 1104 9 0.5752870359516962 1104 10-11 0.5612581597153294 1104 12-13 0.5130082561241238 1104 14-15 0.5293681695132548 1104 16-17 0.5765969211305872 1104 18-19 0.5589502746053228 1104 20-21 0.4970454202279768 1104 22-23 0.5357046154737048 1104 24-25 0.5191829852457914 1104 26-27 0.43315072458047155 1104 28-29 0.4866707034506135 1104 30-31 0.4716271769164351 1104 32-33 0.4451160430034804 1104 34-35 0.46079245921781364 1104 36-37 0.49084090351676934 1104 38-39 0.4463209368005039 1104 40-41 0.4412058138409449 1104 42-43 0.4327659374084476 1104 44-45 0.4101634327102488 1104 46-47 0.4514873597867535 1104 48-49 0.46853874418894037 1104 50-51 0.39774506291548306 1104 52-53 0.4259910139315721 1104 54-55 0.4974777993781103 1104 56-57 0.45838437030024437 1104 58-59 0.4438802666141797 1104 60-61 0.39164144369656384 1104 62-63 0.40152879354273097 1104 64-65 0.3747690912121513 1104 66-67 0.30184176902199766 1104 68-69 0.4005700989030103 1104 70-71 0.3777529667283588 1104 72-73 0.33823059637792596 1104 74-75 0.3356903634809285 1104 76-77 0.29224304196058526 1104 78-79 0.2937468323927739 1104 80-81 0.2677148510183436 1104 82-83 0.24582187793672716 1104 84-85 0.23074847120519593 1104 86-87 0.18349489900754534 1104 88-89 0.20026542386249702 1104 90-91 0.23991804506878012 1104 92-93 0.23833711528798673 1104 94-95 0.1802690795677968 1104 96-97 0.18494640454385802 1104 98-99 0.12305958226897573 1104 100 0.1786095060996935 1105 1 0.41880056194904114 1105 2 0.3722731426156294 1105 3 0.3723991266263553 1105 4 0.325747779686111 1105 5 0.4090824409686107 1105 6 0.35819323650529356 1105 7 0.3910844015318631 1105 8 0.45532937031888565 1105 9 0.4902925724299365 1105 10-11 0.46226035520328423 1105 12-13 0.4779251956519275 1105 14-15 0.5163420180838401 1105 16-17 0.5148583936785442 1105 18-19 0.4752885880308142 1105 20-21 0.5305653761499656 1105 22-23 0.5052646921397255 1105 24-25 0.4478128693865955 1105 26-27 0.433774079609627 1105 28-29 0.4621069553282382 1105 30-31 0.4761192272015933 1105 32-33 0.407220717410091 1105 34-35 0.43083747363439784 1105 36-37 0.49381047775562337 1105 38-39 0.40708247988933977 1105 40-41 0.36649752191200236 1105 42-43 0.3779118593068276 1105 44-45 0.35718543873372255 1105 46-47 0.31172842260935596 1105 48-49 0.42582222823183713 1105 50-51 0.2764711026426525 1105 52-53 0.3747442094035094 1105 54-55 0.45919193241390843 1105 56-57 0.45581752489245275 1105 58-59 0.38701464912007566 1105 60-61 0.28902223508965363 1105 62-63 0.32081027436869647 1105 64-65 0.2882337508928927 1105 66-67 0.31015923003219115 1105 68-69 0.3923531284425863 1105 70-71 0.3778810273490336 1105 72-73 0.3448322127201635 1105 74-75 0.2917486182931128 1105 76-77 0.29859980744901193 1105 78-79 0.24407853596410334 1105 80-81 0.23539113905740905 1105 82-83 0.24998942330200435 1105 84-85 0.154766669825964 1105 86-87 0.24793063695880946 1105 88-89 0.22727118887272724 1105 90-91 0.27488488205596795 1105 92-93 0.2479850491265836 1105 94-95 0.19359440451011167 1105 96-97 0.2360576165603412 1105 98-99 0.24908986074281714 1105 100 0.21693938023831194 1106 1 0.3612356964879666 1106 2 0.38343659440463895 1106 3 0.350206592627746 1106 4 0.2681708811083041 1106 5 0.3297156509311776 1106 6 0.35242923679032856 1106 7 0.37955082149196784 1106 8 0.43964980713527524 1106 9 0.49494973111148965 1106 10-11 0.4044957606758999 1106 12-13 0.37864503182016307 1106 14-15 0.43142392341716373 1106 16-17 0.532152814572207 1106 18-19 0.46205686565154025 1106 20-21 0.5264924236050632 1106 22-23 0.5237546045250383 1106 24-25 0.4495373140015744 1106 26-27 0.38475323574567 1106 28-29 0.39388449349947763 1106 30-31 0.38632423973047736 1106 32-33 0.34415934858703423 1106 34-35 0.40396475669457743 1106 36-37 0.44774281316387743 1106 38-39 0.44356328845213255 1106 40-41 0.4163478701903145 1106 42-43 0.36537754696270497 1106 44-45 0.3684470831080944 1106 46-47 0.37381568374343743 1106 48-49 0.4425254668062166 1106 50-51 0.40585786975461247 1106 52-53 0.29178085001370846 1106 54-55 0.3574582459955522 1106 56-57 0.3789707108329594 1106 58-59 0.3706007033098615 1106 60-61 0.35573193331335773 1106 62-63 0.36133373218945763 1106 64-65 0.373882915045634 1106 66-67 0.33698106503876346 1106 68-69 0.3686252211839758 1106 70-71 0.4296758550250601 1106 72-73 0.37532381405760873 1106 74-75 0.3123996236555655 1106 76-77 0.303552675100466 1106 78-79 0.2694421249471155 1106 80-81 0.1983756246675732 1106 82-83 0.2369548941273365 1106 84-85 0.24196657500769092 1106 86-87 0.2578400230353921 1106 88-89 0.29928508764017536 1106 90-91 0.29028251015488493 1106 92-93 0.2799444934023647 1106 94-95 0.239120575991965 1106 96-97 0.262920966323815 1106 98-99 0.25479551227809694 1106 100 0.2575991569697784 1107 1 0.36734024366003126 1107 2 0.18634782606775602 1107 3 0.3180797521802319 1107 4 0.2742751245921795 1107 5 0.3459090045621238 1107 6 0.3356944018052985 1107 7 0.3601818133968706 1107 8 0.4305870250018913 1107 9 0.46151329657785567 1107 10-11 0.39885440404240313 1107 12-13 0.4372338119494472 1107 14-15 0.37134295626234604 1107 16-17 0.42905001473901194 1107 18-19 0.4467847903063884 1107 20-21 0.4300676930616234 1107 22-23 0.4375894131114393 1107 24-25 0.4096846365281692 1107 26-27 0.36254778111280217 1107 28-29 0.37525896139047177 1107 30-31 0.3824435004460227 1107 32-33 0.30622834144594435 1107 34-35 0.319268332636625 1107 36-37 0.35938888686157355 1107 38-39 0.3752166844869791 1107 40-41 0.3334320725503588 1107 42-43 0.3512118556551016 1107 44-45 0.3971287317166414 1107 46-47 0.2972396796070669 1107 48-49 0.35493405491839525 1107 50-51 0.2468703506820802 1107 52-53 0.2333879768128284 1107 54-55 0.35292826301317604 1107 56-57 0.33878615145442126 1107 58-59 0.30596924807966275 1107 60-61 0.3174754028008451 1107 62-63 0.35598426023285157 1107 64-65 0.34028271707013147 1107 66-67 0.33489021399831387 1107 68-69 0.3328510296432654 1107 70-71 0.3759704785777984 1107 72-73 0.3278354665734824 1107 74-75 0.3265971877855307 1107 76-77 0.2934254532471421 1107 78-79 0.26004206372224914 1107 80-81 0.2614534677039799 1107 82-83 0.25352982134887725 1107 84-85 0.2232325943523037 1107 86-87 0.24637998971890696 1107 88-89 0.3165197915613511 1107 90-91 0.3182972687902499 1107 92-93 0.29949517275152004 1107 94-95 0.2831120819096107 1107 96-97 0.26755654849397814 1107 98-99 0.3152519454059366 1107 100 0.24668785137269467 1108 1 0.2644172991907965 1108 2 0.14329209087045314 1108 3 0.25775746113192 1108 4 0.2470681377940025 1108 5 0.24513435928542293 1108 6 0.2858619241228908 1108 7 0.2928647688195909 1108 8 0.41436403361233687 1108 9 0.39577800321627166 1108 10-11 0.4012835080888877 1108 12-13 0.3234271172397243 1108 14-15 0.3433321309003645 1108 16-17 0.3859258287077907 1108 18-19 0.3199638290053741 1108 20-21 0.3657722264195655 1108 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2316 54-55 -5.591132125607345 2316 56-57 -3.3776521545313756 2316 58-59 -5.14482532737933 2316 60-61 -2.4857166074570998 2316 62-63 -3.0446541009850208 2316 64-65 -2.1944550443329334 2316 66-67 -1.8014529300476525 2316 68-69 -1.740262837729496 2316 70-71 -1.7846116254790374 2316 72-73 -1.6347025100489745 2316 74-75 -1.6905458776500204 2316 76-77 -1.7179440605898613 2316 78-79 -1.6547543310115707 2316 80-81 -1.6376300799326664 2316 82-83 -1.7053158765388403 2316 84-85 -1.5114408605468732 2316 86-87 -1.460263853719919 2316 88-89 -1.5624505584856614 2316 90-91 -2.4007877134953226 2316 92-93 -1.6282237808943947 2316 94-95 -1.5791985617740707 2316 96-97 -1.7842020938904497 2316 98-99 -1.8379087895032633 2316 100 -1.8152070110872032 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13.0 3 9.0 4 10.0 5 40.0 6 519.0 7 6031.0 8 19024.0 9 33993.0 10 45302.0 11 52565.0 12 65439.0 13 87150.0 14 115569.0 15 152711.0 16 198131.0 17 246181.0 18 282491.0 19 323038.0 20 363927.0 21 438681.0 22 567689.0 23 781643.0 24 1062806.0 25 1230238.0 26 1136433.0 27 986074.0 28 925602.0 29 969000.0 30 1097971.0 31 1302108.0 32 1597557.0 33 2021114.0 34 2678934.0 35 3879895.0 36 5759303.0 37 9040158.0 38 1.0641195E7 39 3278889.0 40 5017.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 15.780452934717012 37.75477923216206 28.13490267617699 18.329865156943946 2 18.924962486643025 35.71352141625218 28.16457017033589 17.196945926768905 3 26.653175423482484 26.811037017656535 27.33829178453068 19.197495774330307 4 18.369212151571716 27.473445902196424 26.77925122608415 27.378090720147707 5 19.19537072696439 35.266184133552805 26.82465730564983 18.713787833832974 6 27.367316157574724 27.060682995762363 27.060889252124937 18.511111594537972 7 19.093566345123143 26.980563924007395 35.22804850933176 18.697821221537705 8 27.206587523868546 27.0567813880572 27.069172320175383 18.66745876789887 9 26.732073841621602 26.79000844968884 27.685963446947675 18.791954261741882 10-11 18.43112007243314 31.552620845184677 31.373126841527643 18.64313224085454 12-13 18.437169692284773 27.50727106268339 31.096239116512496 22.959320128519344 14-15 18.473325170394236 35.81946795011881 26.894769555294683 18.812437324192274 16-17 22.841302697693024 27.268499465777353 26.88636341967095 23.00383441685867 18-19 18.782117208907735 31.627561843987706 26.86934893615158 22.720972010952973 20-21 22.878357893192465 31.663216212474428 26.820918946481427 18.637506947851683 22-23 22.671980069649695 27.627813283920684 31.02311647905318 18.677090167376438 24-25 18.58491344662684 31.468033132463024 26.91180125543724 23.035252165472897 26-27 18.575857112256273 27.475144028518482 35.28845787654839 18.66054098267685 28-29 18.608855862568376 27.49808140511189 35.252720538545134 18.640342193774597 30-31 18.602040893541943 27.542620971096742 35.196370560566386 18.658967574794925 32-33 18.68573195149786 27.570777889332465 35.08759131914449 18.655898840025184 34-35 18.685620285526337 27.64903567313248 35.032320661122604 18.633023380218567 36-37 18.70268916179802 27.650105250675878 34.98205449245373 18.665151095072375 38-39 18.716970448905297 27.65648110044313 34.930828801728175 18.695719648923397 40-41 18.734372702368766 27.700110308676518 34.91688457506272 18.648632413891995 42-43 18.782366301595744 27.768176627964863 34.87121223759395 18.578244832845442 44-45 18.840343404344207 27.832088114283394 34.72756063966896 18.600007841703444 46-47 18.824109953789623 27.895037830204956 34.67552671240453 18.60532550360089 48-49 18.93105671280367 27.939860034078013 34.521196952503246 18.60788630061507 50-51 18.96736057284677 28.002815201922733 34.40893377268278 18.620890452547727 52-53 19.107497537486125 28.21941462137007 34.05526999437335 18.61781784677045 54-55 19.596398174532563 28.523438832055238 33.05553848337709 18.82462451003511 56-57 20.191722695920735 29.25991752253112 31.53849488863526 19.009864892912884 58-59 20.51690031474586 29.798420129138037 30.679199162985782 19.005480393130316 60-61 20.64440167129768 30.185901067193043 30.171291637702524 18.998405623806754 62-63 20.81541811332266 30.352761202457916 29.91079176839032 18.921028915829098 64-65 21.047735258763225 30.41575668069083 29.720526046946848 18.815982013599093 66-67 21.266967346696177 30.197196192871278 29.84758630970233 18.688250150730216 68-69 21.436659393454295 29.9247308421414 30.04858337730663 18.590026387097673 70-71 21.544361446025484 29.776318658832736 30.229043657216888 18.450276237924896 72-73 21.711639527678006 29.65438390014617 30.221212975533252 18.412763596642577 74-75 21.888466096764557 29.68989676887346 30.063928648186984 18.357708486175007 76-77 22.227029546694872 29.764281412442685 29.704208586088942 18.3044804547735 78-79 22.473026183126382 30.00562949961467 29.291735912493383 18.229608404765564 80-81 22.799196907397594 30.135744249360023 28.91892490463307 18.14613393860931 82-83 23.103668897524365 30.292497170762445 28.482071583458833 18.121762348254357 84-85 23.302796694543115 30.412447162963215 28.180413470088695 18.104342672404975 86-87 23.572305121130206 30.409236919523288 27.88864238550115 18.129815573845352 88-89 23.890858520150186 30.421962687232124 27.647231860915838 18.039946931701856 90-91 24.167637965475013 30.311255534819896 27.507221226037053 18.013885273668038 92-93 24.377937317207845 30.168967779593014 27.480803934297725 17.97229096890142 94-95 24.596932544141918 30.105328115744644 27.37099671168294 17.9267426284305 96-97 24.75323706238224 30.129272052074175 27.230278413938198 17.887212471605388 98-99 24.902026240225215 30.12300674893811 27.044272774846895 17.93069423598978 100 24.986385063337952 30.13099955945085 26.909934490477923 17.972680886733276 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 205790.0 1 123664.5 2 33690.5 3 24870.0 4 23875.0 5 24206.5 6 25687.5 7 26270.0 8 26145.0 9 26877.0 10 29252.5 11 39225.0 12 310835.0 13 365453.0 14 150956.0 15 156997.0 16 180169.5 17 203709.0 18 228135.0 19 253374.0 20 283149.0 21 324578.0 22 379843.5 23 444279.5 24 515843.5 25 589934.0 26 666722.0 27 758925.5 28 881058.5 29 1017866.5 30 1144792.5 31 1273690.0 32 1385815.0 33 1434006.5 34 1456682.5 35 1500044.0 36 1574377.0 37 1662098.5 38 1723819.5 39 1731829.0 40 1694763.0 41 1668533.0 42 1702922.0 43 1703929.0 44 1682421.5 45 1695066.5 46 1689635.5 47 1689021.0 48 1620618.5 49 1501552.0 50 1438024.5 51 1379546.5 52 1295334.5 53 1208176.5 54 1093641.5 55 959591.5 56 826479.5 57 704640.5 58 605984.0 59 506892.5 60 405469.0 61 343726.0 62 282490.0 63 190474.0 64 118082.5 65 76489.0 66 51174.0 67 38502.0 68 28918.0 69 18962.0 70 12738.5 71 9982.0 72 8704.0 73 7396.0 74 6114.5 75 5431.0 76 4160.5 77 2509.0 78 1539.5 79 1188.5 80 985.5 81 819.0 82 684.5 83 558.5 84 478.5 85 402.0 86 334.5 87 299.5 88 261.5 89 224.0 90 202.5 91 170.0 92 133.0 93 108.0 94 89.5 95 69.5 96 51.5 97 43.0 98 34.0 99 22.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.938909314500476E-4 2 0.0014690873854038872 3 0.0011597034194711481 4 7.432998426811721E-4 5 0.0017162053959287795 6 1.8679786622354062E-4 7 5.642852208836123E-5 8 5.642852208836123E-5 9 4.864527766238037E-5 10-11 0.005275093909708527 12-13 6.0320144301351655E-5 14-15 2.529554438443779E-5 16-17 3.891622212990429E-5 18-19 4.864527766238037E-5 20-21 0.0012939643858193178 22-23 0.016160934144996007 24-25 0.026806466708631325 26-27 0.03702975826215718 28-29 0.04694853037751654 30-31 0.10088933296622364 32-33 0.012828732625122949 34-35 0.006800609817200775 36-37 0.0012034841693672903 38-39 0.0028087783322258424 40-41 0.0023485940055397244 42-43 0.0014525479909986779 44-45 0.001079925164104844 46-47 6.129304985459927E-4 48-49 7.180042982967343E-4 50-51 0.185321968499264 52-53 0.2999662401773023 54-55 0.16057514284685787 56-57 0.1854143945268225 58-59 0.18367775811427553 60-61 0.1817192992355881 62-63 0.18201214380711564 64-65 0.17452077104710906 66-67 0.18260075166683043 68-69 0.16315917999628352 70-71 0.17147654957099728 72-73 0.1647226392203524 74-75 0.15796483724749452 76-77 0.1502847208101579 78-79 0.14647676847474678 80-81 0.14899951257431782 82-83 0.14456500906261524 84-85 0.13440884799226346 86-87 0.13438257954232577 88-89 0.13749295859605837 90-91 0.14759463695542827 92-93 0.1428332371778345 94-95 0.1427087052670188 96-97 0.14047296830565578 98-99 0.13751533542378308 100 0.13647919100957437 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5.139245E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.737585925471873 #Duplication Level Percentage of deduplicated Percentage of total 1 67.5924910371801 16.044825639117498 2 14.151705159190984 6.718546344164792 3 5.96758914204946 4.249684800819361 4 3.0664854783777633 2.911638501288155 5 1.8631958756971434 2.2113886097672872 6 1.197992648611049 1.7062472060693044 7 0.8250228412385876 1.3708835409064537 8 0.6115828529391621 1.1614000417750872 9 0.44244232833099106 0.9452261507240464 >10 3.4338098942680904 16.631099558143553 >50 0.45076200416555645 7.509165715384435 >100 0.3423930956687089 14.663557152901955 >500 0.02983733692980899 4.886889947788639 >1k 0.024231190054782033 10.365894403018379 >5k 3.2793954366002555E-4 0.5049800248065046 >10k+ 1.311757539550742E-4 8.118572363324386 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3588039 6.981646136737984 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 230826 0.44914379446786445 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 88062 0.1713520176601816 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 87195 0.16966499943085026 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.04278060298740379 0.0 1.9458111064952148E-6 5.837433319485644E-6 0.0 2 0.04280979015400122 0.0 1.9458111064952148E-6 9.729055532476075E-6 0.0 3 0.04281562758732071 0.0 1.9458111064952148E-6 9.729055532476075E-6 0.0 4 0.0428175733984272 0.0 1.9458111064952148E-6 1.5566488851961718E-5 0.0 5 0.0428175733984272 0.0 1.9458111064952148E-6 1.945811106495215E-5 0.0 6 0.0428175733984272 0.0 1.9458111064952148E-6 2.3349733277942577E-5 0.0 7 0.042819519209533696 0.0 1.9458111064952148E-6 3.1132977703923436E-5 0.0 8 0.042819519209533696 0.0 1.9458111064952148E-6 3.697041102340908E-5 1.9458111064952148E-6 9 0.042821465020640194 0.0 1.9458111064952148E-6 3.89162221299043E-5 1.9458111064952148E-6 10-11 0.04282730245395967 0.0 1.9458111064952148E-6 4.572656100263755E-5 1.9458111064952148E-6 12-13 0.04285551671500386 0.0 1.9458111064952148E-6 6.0320144301351655E-5 1.9458111064952148E-6 14-15 0.04288275807049479 0.0 1.9458111064952148E-6 6.810338872733252E-5 2.918716659742822E-6 16-17 0.04290708070932598 0.0 1.9458111064952148E-6 7.685953870656099E-5 3.8916222129904295E-6 18-19 0.04293626787592341 9.729055532476074E-7 1.9458111064952148E-6 8.075116091955142E-5 3.8916222129904295E-6 20-21 0.042968373759180584 1.9458111064952148E-6 1.9458111064952148E-6 8.464278313254184E-5 3.8916222129904295E-6 22-23 0.04305398944786637 1.9458111064952148E-6 1.9458111064952148E-6 8.756149979228466E-5 3.8916222129904295E-6 24-25 0.04323592278632367 2.918716659742822E-6 1.9458111064952148E-6 9.339893311177031E-5 7.783244425980859E-6 26-27 0.04401230141781526 3.8916222129904295E-6 1.9458111064952148E-6 9.826346087800836E-5 7.783244425980859E-6 28-29 0.04706236032724651 3.8916222129904295E-6 1.9458111064952148E-6 1.0118217753775116E-4 7.783244425980859E-6 30-31 0.055824347739794465 3.8916222129904295E-6 1.9458111064952148E-6 1.0312798864424639E-4 8.756149979228468E-6 32-33 0.0755743304707209 5.837433319485644E-6 1.9458111064952148E-6 1.0993832751697963E-4 9.729055532476075E-6 34-35 0.11289304168219261 7.783244425980859E-6 1.9458111064952148E-6 1.186944774962081E-4 9.729055532476075E-6 36-37 0.1753088245452396 7.783244425980859E-6 1.9458111064952148E-6 1.2064028860270331E-4 9.729055532476075E-6 38-39 0.27016127855356187 7.783244425980859E-6 1.9458111064952148E-6 1.2842353302868417E-4 9.729055532476075E-6 40-41 0.4023635767510597 7.783244425980859E-6 1.9458111064952148E-6 1.342609663481698E-4 1.3620677745466504E-5 42-43 0.5763414664994566 7.783244425980859E-6 1.9458111064952148E-6 1.4009839966765548E-4 1.3620677745466504E-5 44-45 0.7986134150055115 8.756149979228468E-6 1.9458111064952148E-6 1.5080036075337913E-4 1.3620677745466504E-5 46-47 1.0733716722981683 9.729055532476075E-6 1.9458111064952148E-6 1.615023218391028E-4 1.7512299958456932E-5 48-49 1.403885201036339 1.0701961085723682E-5 1.9458111064952148E-6 1.6928556626508368E-4 1.7512299958456932E-5 50-51 1.7872975505156887 1.2647772192218896E-5 1.9458111064952148E-6 1.7998752735080736E-4 1.7512299958456932E-5 52-53 2.218048565499407 1.5566488851961718E-5 1.9458111064952148E-6 1.85824960670293E-4 2.1403922171447363E-5 54-55 2.7021829081898217 1.5566488851961718E-5 1.9458111064952148E-6 1.9944563841575952E-4 2.1403922171447363E-5 56-57 3.239868891247644 1.5566488851961718E-5 1.9458111064952148E-6 2.0625597728849278E-4 2.1403922171447363E-5 58-59 3.8286645217342237 1.5566488851961718E-5 1.9458111064952148E-6 2.0917469394823559E-4 2.1403922171447363E-5 60-61 4.466571451643189 1.7512299958456932E-5 1.9458111064952148E-6 2.1890374948071165E-4 2.1403922171447363E-5 62-63 5.141953730557699 1.7512299958456932E-5 1.9458111064952148E-6 2.3349733277942575E-4 2.1403922171447363E-5 64-65 5.851058861758878 1.7512299958456932E-5 1.9458111064952148E-6 2.383618605456638E-4 2.1403922171447363E-5 66-67 6.5965633084237085 1.848520551170454E-5 1.9458111064952148E-6 2.4419929386514946E-4 2.4322638831190186E-5 68-69 7.377906676953521 1.945811106495215E-5 1.9458111064952148E-6 2.519825382911303E-4 2.724135549093301E-5 70-71 8.189486004267163 1.945811106495215E-5 1.9458111064952148E-6 2.607386882703588E-4 2.9187166597428222E-5 72-73 9.023401297272265 1.945811106495215E-5 1.9458111064952148E-6 2.62684499376854E-4 3.405169436366626E-5 74-75 9.877266991552261 1.945811106495215E-5 1.9458111064952148E-6 2.67549027143092E-4 3.599750547016147E-5 76-77 10.751365229717594 2.237682772469497E-5 1.9458111064952148E-6 2.7046774380283485E-4 3.891622212990429E-5 78-79 11.643229696190783 2.62684499376854E-5 1.9458111064952148E-6 2.753322715690729E-4 4.1834938789647114E-5 80-81 12.55117823727026 3.210588325717105E-5 1.9458111064952148E-6 2.801967993353109E-4 4.2807844342894726E-5 82-83 13.47283307178389 3.405169436366626E-5 1.9458111064952148E-6 2.8214261044180614E-4 4.2807844342894726E-5 84-85 14.4062250388919 3.891622212990429E-5 1.9458111064952148E-6 2.870071382080442E-4 4.2807844342894726E-5 86-87 15.349761492203623 5.25368998753708E-5 1.9458111064952148E-6 2.9089876042103456E-4 4.2807844342894726E-5 88 16.06400161891484 6.810338872733252E-5 1.9458111064952148E-6 2.977090992937679E-4 4.475365544938994E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 451485 0.0 86.98206 8 AGTGGTA 454695 0.0 86.50003 5 AAGCAGT 466455 0.0 86.28858 1 TGGTATC 453605 0.0 86.09645 7 GCAGTGG 467335 0.0 85.725395 3 GTATCAA 455535 0.0 85.717255 9 GTGGTAT 458520 0.0 85.515465 6 AGCAGTG 480970 0.0 83.67866 2 CAGTGGT 488060 0.0 81.050224 4 ATCAACG 447860 0.0 45.67515 10-11 CAACGCA 448710 0.0 45.601833 12-13 ACGCAGA 453970 0.0 45.21571 14-15 AGAGTAC 459790 0.0 43.941837 18-19 TCAACGC 449535 0.0 43.712513 12-13 AACGCAG 450620 0.0 43.39122 14-15 TATCAAC 451330 0.0 43.17602 10-11 CGCAGAG 455275 0.0 42.740685 16-17 AGTACTT 477185 0.0 42.36592 20-21 GCAGAGT 480060 0.0 42.153744 16-17 GAGTACT 453125 0.0 41.990414 20-21 >>END_MODULE