FastQCFastQC Report
Thu 26 May 2016
SRR1660683_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660683_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51392450
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30872466.007197555282926No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2414350.46978690449667215No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1221280.23763801881404759No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT836710.16280796109156112No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT831500.16179419350507712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3981000.087.007228
TGGTATC4026000.085.960637
AGTGGTA4082550.085.739065
GTATCAA4020550.085.537939
GCAGTGG4144400.084.802843
AAGCAGT3971700.084.786781
GTGGTAT4119250.084.768836
AGCAGTG4256350.082.356122
CAGTGGT4411950.079.486714
ATCAACG3904950.045.87097510-11
CAACGCA3916750.045.7732912-13
ACGCAGA4032100.045.00504314-15
TCAACGC3915800.043.83090212-13
AGAGTAC4154950.043.5022718-19
AACGCAG3980800.043.1761214-15
TATCAAC3981300.043.13593710-11
CGCAGAG4045400.042.45937316-17
AGTACTT4330700.041.7211620-21
GAGTACT4079000.041.61485720-21
GCAGAGT4349450.041.59716416-17
Produced by FastQC (version 0.11.5)