##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660683_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51392450 Sequences flagged as poor quality 0 Sequence length 100 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.58009641494033 31.0 30.0 34.0 16.0 34.0 2 30.608694856929375 31.0 30.0 34.0 25.0 34.0 3 31.401773314951903 31.0 31.0 34.0 28.0 34.0 4 35.139180871898496 37.0 35.0 37.0 32.0 37.0 5 35.41259531312479 37.0 35.0 37.0 33.0 37.0 6 35.56235859547463 37.0 35.0 37.0 33.0 37.0 7 35.57466727116532 37.0 35.0 37.0 33.0 37.0 8 35.617929267820465 37.0 35.0 37.0 33.0 37.0 9 37.27587853857911 39.0 37.0 39.0 34.0 39.0 10-11 37.203457132321965 39.0 37.0 39.0 33.5 39.0 12-13 37.04696749230675 39.0 37.0 39.0 33.5 39.0 14-15 38.38296604851491 40.0 38.0 41.0 33.0 41.0 16-17 38.50339587818833 40.0 38.0 41.0 34.0 41.0 18-19 38.49357877470329 40.0 38.0 41.0 34.0 41.0 20-21 38.423042684285335 40.0 38.0 41.0 34.0 41.0 22-23 38.212039492182214 40.0 37.5 41.0 33.5 41.0 24-25 38.24849912779017 40.0 37.5 41.0 34.0 41.0 26-27 38.19183111527082 40.0 38.0 41.0 33.5 41.0 28-29 38.116266406836026 40.0 37.5 41.0 33.0 41.0 30-31 38.008807295624166 40.0 37.0 41.0 33.0 41.0 32-33 37.87856477945691 40.0 37.0 41.0 33.0 41.0 34-35 37.69226128546119 40.0 37.0 41.0 32.5 41.0 36-37 37.48452152018439 39.5 37.0 41.0 32.0 41.0 38-39 37.010770648217786 39.0 35.0 41.0 31.0 41.0 40-41 36.86628130202004 39.0 35.0 40.0 31.0 41.0 42-43 36.70727077615486 39.0 35.0 40.0 31.0 41.0 44-45 36.829337830751406 39.0 35.0 40.5 31.0 41.0 46-47 36.75287444945707 39.0 35.0 41.0 31.0 41.0 48-49 36.62985841305483 39.0 35.0 40.5 30.5 41.0 50-51 36.426108523722846 39.0 35.0 40.0 30.0 41.0 52-53 36.22069697008024 39.0 35.0 40.0 29.5 41.0 54-55 35.824067766763406 39.0 35.0 40.5 27.0 41.0 56-57 35.28011638869134 39.0 34.5 40.5 24.5 41.0 58-59 35.03292053599313 38.0 34.0 40.0 23.5 41.0 60-61 34.85320582108851 38.0 34.0 40.0 23.0 41.0 62-63 34.55355884959756 38.0 34.0 40.0 22.5 41.0 64-65 34.29517496247017 37.0 34.0 40.0 22.0 41.0 66-67 34.10433969386554 37.0 33.0 40.0 22.0 41.0 68-69 33.868264443512615 36.0 33.0 39.5 22.0 41.0 70-71 33.5974095903192 36.0 33.0 39.0 22.0 41.0 72-73 33.281690005049384 35.5 33.0 39.0 22.0 40.5 74-75 32.9206283218644 35.0 32.0 38.0 20.5 40.0 76-77 31.131811842011814 33.5 30.0 36.0 18.5 39.0 78-79 32.16445379622883 35.0 31.5 37.0 20.0 39.0 80-81 32.10971950159994 35.0 32.0 36.5 20.0 39.0 82-83 31.845460276752714 35.0 32.0 36.0 19.0 38.0 84-85 31.57882278428057 35.0 32.0 36.0 19.0 37.0 86-87 31.32908814427022 35.0 32.0 35.0 18.0 37.0 88-89 31.113514417000943 35.0 31.5 35.0 18.0 36.0 90-91 30.923506273781463 35.0 31.0 35.0 17.0 36.0 92-93 30.72884501517246 34.5 31.0 35.0 16.5 36.0 94-95 30.565480386710497 34.0 31.0 35.0 15.0 35.5 96-97 30.439772203893764 34.0 31.0 35.0 14.0 35.0 98-99 30.326839584413662 34.0 31.0 35.0 12.0 35.0 100 30.24566324430923 34.0 31.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.820553248157683 1101 2 2.040583864167967 1101 3 1.3187902145068762 1101 4 1.1044795122211113 1101 5 0.6675756597479037 1101 6 0.4565927226515285 1101 7 0.389933035300551 1101 8 0.38023987741445353 1101 9 0.3838069986285859 1101 10-11 0.37298877016767307 1101 12-13 0.6132777950196129 1101 14-15 0.657954491507823 1101 16-17 0.4668885998872767 1101 18-19 0.4522328128090294 1101 20-21 0.4270522194922606 1101 22-23 0.4002432532581608 1101 24-25 0.42631627392377425 1101 26-27 0.4044492900163732 1101 28-29 0.3803976183058424 1101 30-31 0.3506247800109108 1101 32-33 0.3547520771600077 1101 34-35 0.34729558683306294 1101 36-37 0.31439615322768333 1101 38-39 0.38787871632771953 1101 40-41 0.4047025340078534 1101 42-43 0.3999707570267006 1101 44-45 0.2730511747193347 1101 46-47 0.2676125548157131 1101 48-49 0.23023502147239583 1101 50-51 0.29800424826671446 1101 52-53 0.2750442102232782 1101 54-55 0.23719818421101735 1101 56-57 0.2127068657727591 1101 58-59 0.22509119789935994 1101 60-61 0.2226629432569709 1101 62-63 0.2878642244567047 1101 64-65 0.3317948865573399 1101 66-67 0.2693769005690996 1101 68-69 0.18305476945696597 1101 70-71 0.0784600127148849 1101 72-73 0.13086298588184064 1101 74-75 0.07251278206414469 1101 76-77 0.08194924505142609 1101 78-79 0.058857422056505015 1101 80-81 0.04801974680463417 1101 82-83 0.1786100304812912 1101 84-85 0.07297337121022807 1101 86-87 0.07598705548679874 1101 88-89 -0.039365630449463396 1101 90-91 -0.020276389402837935 1101 92-93 0.06608775933506195 1101 94-95 -0.0028259578190130696 1101 96-97 0.04867663809953626 1101 98-99 0.01649507577936049 1101 100 -0.014356307488576192 1102 1 2.782241411972951 1102 2 2.025303438635877 1102 3 1.2986107420788997 1102 4 1.058023338238101 1102 5 0.6229362681934916 1102 6 0.4444642592495853 1102 7 0.33255371580550985 1102 8 0.33139625099820336 1102 9 0.32768495789693475 1102 10-11 0.28982731779900917 1102 12-13 0.5473054064155889 1102 14-15 0.6164827823092054 1102 16-17 0.32578759806917645 1102 18-19 0.3670091668936166 1102 20-21 0.36149966967288805 1102 22-23 0.34679781410174115 1102 24-25 0.3345674628489803 1102 26-27 0.3338912452422562 1102 28-29 0.3172179906501711 1102 30-31 0.2919720325227857 1102 32-33 0.30192934921801395 1102 34-35 0.2986871170330474 1102 36-37 0.35323836158162436 1102 38-39 0.3722077331690343 1102 40-41 0.34001631057630277 1102 42-43 0.32065235847620244 1102 44-45 0.3204098455966502 1102 46-47 0.25146146432719974 1102 48-49 0.19123055212118345 1102 50-51 0.2588127421183586 1102 52-53 0.18943302887621627 1102 54-55 0.2593227634787496 1102 56-57 0.23127736704994106 1102 58-59 0.2328568678450793 1102 60-61 0.18084132905192263 1102 62-63 0.2242014008051143 1102 64-65 0.1619227786531212 1102 66-67 0.12807946464445763 1102 68-69 0.11204540956063624 1102 70-71 0.10640723445330025 1102 72-73 0.05489107670208426 1102 74-75 0.03876855043964156 1102 76-77 0.04167582533501246 1102 78-79 0.187043250223617 1102 80-81 0.2057765782880736 1102 82-83 0.1635463669970072 1102 84-85 0.14369134804419303 1102 86-87 0.13595985987568326 1102 88-89 0.10168711330584657 1102 90-91 0.021940099384064382 1102 92-93 0.13331370717205715 1102 94-95 0.07252587411704425 1102 96-97 0.06720533309450971 1102 98-99 -0.007321721000487003 1102 100 -0.10468971387983217 1103 1 2.7754039805547244 1103 2 1.997779822601192 1103 3 1.27325425284522 1103 4 1.0563981738921342 1103 5 0.6243119735172442 1103 6 0.41213700504263073 1103 7 0.28616219450765357 1103 8 0.2783770872410898 1103 9 0.29610882342988987 1103 10-11 0.29126215451579185 1103 12-13 0.5398940543607509 1103 14-15 0.5891216058012532 1103 16-17 0.34612653609827504 1103 18-19 0.25790694821818505 1103 20-21 0.24133520661374774 1103 22-23 0.26303713368461956 1103 24-25 0.32120963094087784 1103 26-27 0.3025715424036193 1103 28-29 0.31304715775986125 1103 30-31 0.29788365515089055 1103 32-33 0.2930081785616494 1103 34-35 0.32695323405560117 1103 36-37 0.252278173734382 1103 38-39 0.2915155593530585 1103 40-41 0.2753849356785807 1103 42-43 0.21127201508218008 1103 44-45 0.23842746598656817 1103 46-47 0.14764919257714837 1103 48-49 0.1579641918421686 1103 50-51 0.19082324384446991 1103 52-53 0.17552594665041 1103 54-55 0.22002025174864315 1103 56-57 0.17520393606604046 1103 58-59 0.13773097073546836 1103 60-61 0.16842439353980154 1103 62-63 0.17962889078194877 1103 64-65 0.13399324871725327 1103 66-67 0.06544365858070478 1103 68-69 0.09898492721019636 1103 70-71 0.032033526292657655 1103 72-73 -0.009841520101709023 1103 74-75 -0.009566495086623661 1103 76-77 -0.10507565677217556 1103 78-79 0.10438667519496647 1103 80-81 0.09840078147375664 1103 82-83 0.11612978375931604 1103 84-85 0.09647987600966701 1103 86-87 0.11633271890529784 1103 88-89 0.1101734378771333 1103 90-91 0.08918540427114507 1103 92-93 0.07852392139633935 1103 94-95 0.09693240096041578 1103 96-97 0.07744383379395714 1103 98-99 -0.009492320267362686 1103 100 0.017312381293400847 1104 1 2.7106272130891576 1104 2 1.9571234404834463 1104 3 1.2477088852224156 1104 4 1.0106778252825137 1104 5 0.5950239535870168 1104 6 0.3934834348698999 1104 7 0.31792857227819127 1104 8 0.3019576870621563 1104 9 0.33245859900470265 1104 10-11 0.25401261847176926 1104 12-13 0.5220892016716903 1104 14-15 0.532164849885902 1104 16-17 0.3371889988510688 1104 18-19 0.29145870150364317 1104 20-21 0.29587113746116955 1104 22-23 0.2885461162473817 1104 24-25 0.2933987763115695 1104 26-27 0.26003112352985625 1104 28-29 0.2547737623049855 1104 30-31 0.2504966097101189 1104 32-33 0.28830032813037576 1104 34-35 0.2841220995365177 1104 36-37 0.2289279544327698 1104 38-39 0.2825996719963868 1104 40-41 0.3001842215301451 1104 42-43 0.2990532497587779 1104 44-45 0.21842468603568221 1104 46-47 0.2629042460132922 1104 48-49 0.25934550902169917 1104 50-51 0.23684730629275919 1104 52-53 0.2646944510143143 1104 54-55 0.24488288856060336 1104 56-57 0.2290929878287642 1104 58-59 0.1397390604406752 1104 60-61 0.13071075136746657 1104 62-63 0.19776023654812747 1104 64-65 0.19809200112765524 1104 66-67 0.1707392273746393 1104 68-69 0.16250452877944355 1104 70-71 0.13095029792910395 1104 72-73 0.10572297576543832 1104 74-75 0.09577561054680217 1104 76-77 0.07750073132710966 1104 78-79 0.15935756721514593 1104 80-81 0.19885540307071636 1104 82-83 0.20915837288655936 1104 84-85 0.13650452439815552 1104 86-87 0.09252968658568506 1104 88-89 0.1624089777518698 1104 90-91 0.2005505842269102 1104 92-93 0.271392005114798 1104 94-95 0.3032715770687062 1104 96-97 0.22177467050164168 1104 98-99 0.21664578886436914 1104 100 0.21857175920728977 1105 1 2.6641284277113435 1105 2 1.9262905321282169 1105 3 1.1974425848784236 1105 4 1.0040604244504863 1105 5 0.5541817569354563 1105 6 0.3564393579356633 1105 7 0.2428555115023272 1105 8 0.22732293109950064 1105 9 0.270955828395131 1105 10-11 0.23421744867971483 1105 12-13 0.4315491947556822 1105 14-15 0.4968439311515027 1105 16-17 0.31090465793953825 1105 18-19 0.2474096943487325 1105 20-21 0.2433608185550682 1105 22-23 0.22279455047879537 1105 24-25 0.2284109051492038 1105 26-27 0.25483306395624084 1105 28-29 0.23449599600752435 1105 30-31 0.18434261669577978 1105 32-33 0.20491665148639981 1105 34-35 0.24013678741142286 1105 36-37 0.28204573584241643 1105 38-39 0.2613813761733681 1105 40-41 0.2423243777201094 1105 42-43 0.21257523158802627 1105 44-45 0.20129254185376766 1105 46-47 0.1783398517734085 1105 48-49 0.2349052724028695 1105 50-51 0.2372959447138996 1105 52-53 0.2239986031239951 1105 54-55 0.17581014054454158 1105 56-57 0.2192916457747387 1105 58-59 0.1897259553622277 1105 60-61 0.161621412428822 1105 62-63 0.23752287090811564 1105 64-65 0.21042507589445592 1105 66-67 0.14638732762850282 1105 68-69 0.12718266499560826 1105 70-71 0.12135579501423877 1105 72-73 0.09327712410240707 1105 74-75 0.11263298340922034 1105 76-77 0.11484649422274629 1105 78-79 0.2114453626306414 1105 80-81 0.21421193150710138 1105 82-83 0.18230549579550015 1105 84-85 0.16304573981050652 1105 86-87 0.16695271258920386 1105 88-89 0.20715930924155046 1105 90-91 0.21190524901525265 1105 92-93 0.2146854362205346 1105 94-95 0.21030033520381508 1105 96-97 0.24321901965079462 1105 98-99 0.21273137193726654 1105 100 0.21045253634828143 1106 1 2.5999278556613454 1106 2 1.8808240680976702 1106 3 1.165745996811026 1106 4 0.9706097497984203 1106 5 0.559563880649165 1106 6 0.39272946294193645 1106 7 0.24955285924424686 1106 8 0.2633302850883723 1106 9 0.2567574003742692 1106 10-11 0.21330433985091446 1106 12-13 0.4575524338051764 1106 14-15 0.4511570399174403 1106 16-17 0.2955753500456453 1106 18-19 0.2365578885188313 1106 20-21 0.22759318001552487 1106 22-23 0.21624432725196385 1106 24-25 0.22825727350777214 1106 26-27 0.20986090590778872 1106 28-29 0.21598892130544556 1106 30-31 0.25365567871575223 1106 32-33 0.26228943781748626 1106 34-35 0.24981837919364835 1106 36-37 0.32047883121173726 1106 38-39 0.2771696052915331 1106 40-41 0.33188918208790597 1106 42-43 0.3553834769438069 1106 44-45 0.2506052318218721 1106 46-47 0.23253690230490776 1106 48-49 0.2468244098046597 1106 50-51 0.27408972321475034 1106 52-53 0.23364917484216363 1106 54-55 0.15583107416781417 1106 56-57 0.15815671104387263 1106 58-59 0.16089667112029105 1106 60-61 0.16070332528450848 1106 62-63 0.19710642598089834 1106 64-65 0.1401286074096859 1106 66-67 0.06920139109705303 1106 68-69 0.11728671853231276 1106 70-71 0.09608233978959646 1106 72-73 0.09504955833306639 1106 74-75 0.14010851160006865 1106 76-77 0.05556114852085514 1106 78-79 0.19534845348137253 1106 80-81 0.2076296091010832 1106 82-83 0.1844263833734452 1106 84-85 0.17491608443650009 1106 86-87 0.17354637788833926 1106 88-89 0.18194942635068045 1106 90-91 0.18744204723491364 1106 92-93 0.23700296589978365 1106 94-95 0.23413953249032815 1106 96-97 0.2457707803671063 1106 98-99 0.23195194878333325 1106 100 0.2617200452872943 1107 1 0.6880364742598388 1107 2 0.9949232938428025 1107 3 0.7543306511288037 1107 4 0.7704262511048157 1107 5 0.4276154644813204 1107 6 0.30688002294395744 1107 7 0.19936666087879473 1107 8 0.21033360585733618 1107 9 0.26039468611237737 1107 10-11 0.12601666776400577 1107 12-13 0.43267350748278943 1107 14-15 0.3974558180241914 1107 16-17 0.26669023814908854 1107 18-19 0.21685926605708516 1107 20-21 0.2491116717155748 1107 22-23 0.19927910461456833 1107 24-25 0.20904686731694966 1107 26-27 0.1888438877128067 1107 28-29 0.19910013399850612 1107 30-31 0.17517074952598222 1107 32-33 0.23340663561068453 1107 34-35 0.16726616302957353 1107 36-37 0.25714897676215287 1107 38-39 0.2624178765938012 1107 40-41 0.20391144569816788 1107 42-43 0.27921681760437167 1107 44-45 0.2555780044663081 1107 46-47 0.23251610845919402 1107 48-49 0.24738567702518566 1107 50-51 0.1973687965846196 1107 52-53 0.25426473284857565 1107 54-55 0.20566862322316837 1107 56-57 0.2025605291073873 1107 58-59 0.19831518784490498 1107 60-61 0.20167153328635123 1107 62-63 0.2635932497734146 1107 64-65 0.21765421962533082 1107 66-67 0.16032111760058143 1107 68-69 0.17990111513550744 1107 70-71 0.13784996330816313 1107 72-73 0.08962112130679145 1107 74-75 0.006049505986986503 1107 76-77 -0.04207161713550178 1107 78-79 0.16266381831354693 1107 80-81 0.19122816085425143 1107 82-83 0.1286297758606132 1107 84-85 0.14682271370674727 1107 86-87 0.1647728280557068 1107 88-89 0.18830122914567582 1107 90-91 0.18591467801547878 1107 92-93 0.2044199300309728 1107 94-95 0.2173746509608172 1107 96-97 0.2681308775072324 1107 98-99 0.22306359070895354 1107 100 0.2280302183059888 1108 1 0.8871001243522301 1108 2 1.086254683617799 1108 3 0.7686984262045868 1108 4 0.7812915758521513 1108 5 0.39983355832575285 1108 6 0.24978262445108612 1108 7 0.17424445225221774 1108 8 0.17145549396995818 1108 9 0.1514656735253439 1108 10-11 0.09162551438191713 1108 12-13 0.38009157246878544 1108 14-15 0.3753479744160586 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-1.3490817070596108 2316 100 -1.3087666341112723 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 44.0 7 823.0 8 1115.0 9 502.0 10 264.0 11 293.0 12 569.0 13 2587.0 14 11167.0 15 31815.0 16 58724.0 17 87496.0 18 115885.0 19 151722.0 20 203493.0 21 277127.0 22 401741.0 23 606951.0 24 915866.0 25 1116312.0 26 1049561.0 27 929342.0 28 916058.0 29 1003563.0 30 1164809.0 31 1399319.0 32 1705922.0 33 2140239.0 34 2820792.0 35 4062887.0 36 6086177.0 37 9805093.0 38 1.1369743E7 39 2951159.0 40 3290.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.06513887691371 36.1635695441764 28.632921892832986 18.138369686076906 2 19.54963676750201 35.05275135084102 28.33135846393558 17.06625341772138 3 25.907091411287062 27.5631284361808 27.638353493557904 18.89142665897423 4 19.13840105187437 28.08386111352662 26.99252569185796 25.78521214274105 5 19.391486492665752 34.75846938606741 27.076488861690773 18.773555259576067 6 26.450858054052688 27.625639563788067 27.283900650776527 18.639601731382722 7 19.1731703781392 27.526126892179686 34.48297366636539 18.81772906331572 8 26.15140356219639 27.585752381916024 27.407944163004487 18.854899892883097 9 25.8222306973106 27.30393861355121 27.951115387571523 18.92271530156667 10-11 18.58844246577075 31.538570354205724 31.096437317154564 18.77654986286896 12-13 18.589656625086576 28.023075744705505 30.8481986350077 22.539068995200214 14-15 18.60339991574638 35.29392741540829 27.14592026649829 18.95675240234704 16-17 22.40810474124923 27.797794720240592 27.178895283550137 22.615205254960035 18-19 18.86428356694417 31.609213026427035 27.164129166832872 22.362374239795923 20-21 22.45012253745443 31.626114341698052 27.116690292056518 18.807072828791 22-23 22.275087103998583 28.093149410451772 30.812278625659097 18.819484859890544 24-25 18.72916312487908 31.464248880714045 27.152421459974512 22.654166534432363 26-27 18.76337593965102 27.918648699489566 34.49493011298797 18.82304524787144 28-29 18.7563390888555 28.000121652131682 34.441095140820416 18.8024441181924 30-31 18.806642387882324 28.025057590547338 34.365406247428176 18.80289377414216 32-33 18.862976061882776 28.07152775141458 34.248183725070625 18.817312461632017 34-35 18.856045186170782 28.090071869409535 34.240914124943586 18.812968819476097 36-37 18.889725047161594 28.10076771977207 34.18776493434347 18.821742298722867 38-39 18.94341826916037 28.149267710695312 34.119093712784824 18.788220307359484 40-41 18.92115833779816 28.185768002625288 34.11260723489751 18.78046642467904 42-43 18.948738192927973 28.229144552275788 34.07417647439345 18.747940780402793 44-45 18.975421219479546 28.294064379240346 33.971525163609684 18.75898923767042 46-47 19.027752698273588 28.365725134514786 33.8910829131003 18.71543925411133 48-49 19.083060256229054 28.421437636452 33.73938716383092 18.756114943488026 50-51 19.092561236416326 28.47690518011986 33.59811845340395 18.832415130059868 52-53 19.208130610726624 28.66232557617998 33.262180781884716 18.86736303120868 54-55 19.577227435864806 28.97028453307869 32.383573264816775 19.06891476623973 56-57 20.067781584848554 29.63246257772215 31.00477979921512 19.294976038214173 58-59 20.290849496511537 30.133328003816377 30.21247152526122 19.36335097441086 60-61 20.31506326483604 30.508996789603458 29.720969215861125 19.454970729699372 62-63 20.358267620982488 30.689675622059653 29.474504712077266 19.477552044880593 64-65 20.385094167549646 30.874631500008253 29.216157743839727 19.524116588602375 66-67 20.396287476992303 30.780916520968688 29.272642020623806 19.5501539814152 68-69 20.344166358362976 30.589961720006904 29.45425829214985 19.611613629480274 70-71 20.235061190862886 30.491133795017433 29.58485453327466 19.688950480845016 72-73 20.13567789494445 30.38302816866805 29.59742900693213 19.883864929455367 74-75 20.072726535409238 30.43315461210603 29.430914563673433 20.063204288811306 76-77 20.08461693685307 30.447268457591708 29.295204907274453 20.17290969828077 78-79 20.067753648090527 30.66315965010186 28.922307146257907 20.3467795555497 80-81 20.097932642963997 30.779537515651832 28.618513276070594 20.504016565313574 82-83 20.144562471598494 30.911515661863987 28.227828047529147 20.71609381900838 84-85 20.076284758847972 30.989570963422935 27.99165658385131 20.942487693877784 86-87 20.073351251483583 30.987574028194707 27.744348278383974 21.194726441937735 88-89 20.118071254686992 30.98837295584475 27.553292463974127 21.340263325494128 90-91 20.152391336413924 30.845222522895238 27.454251366358413 21.54813477433243 92-93 20.13981851906595 30.666596499290037 27.495459162781387 21.698125818862625 94-95 20.167433675010905 30.576975937363805 27.411860397096493 21.8437299905288 96-97 20.139153309534283 30.549170622967083 27.311978909305008 21.99969715819363 98-99 20.091281534758306 30.478948100822183 27.221142809447723 22.208627554971788 100 20.0186330871558 30.39480118188567 27.211549556403714 22.375016174554823 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 213092.0 1 131821.0 2 39291.5 3 26637.0 4 25039.0 5 25072.0 6 26281.0 7 26648.0 8 26254.5 9 26824.0 10 29331.5 11 41429.5 12 285171.5 13 327955.0 14 132781.5 15 139172.5 16 160975.5 17 183830.5 18 208425.5 19 234185.5 20 266167.5 21 311406.0 22 371876.5 23 441966.5 24 518297.5 25 597716.5 26 680030.5 27 777869.5 28 905938.5 29 1048044.5 30 1178359.0 31 1309777.0 32 1420363.5 33 1461674.0 34 1475763.5 35 1512089.0 36 1582797.0 37 1666382.0 38 1724684.0 39 1736599.0 40 1703638.0 41 1681863.5 42 1723980.5 43 1725516.0 44 1702337.5 45 1713293.5 46 1696177.5 47 1683172.0 48 1609342.0 49 1485805.0 50 1427042.0 51 1374708.5 52 1291691.5 53 1206373.5 54 1091143.5 55 954432.0 56 817707.0 57 694075.5 58 596601.0 59 496430.5 60 392943.5 61 332974.5 62 272706.0 63 180441.5 64 109264.5 65 69684.5 66 45907.5 67 34387.0 68 25507.5 69 16052.5 70 10284.5 71 7982.5 72 6909.0 73 5669.5 74 4635.5 75 4124.5 76 2920.0 77 1395.5 78 579.0 79 379.5 80 292.0 81 199.0 82 125.5 83 81.0 84 62.0 85 49.0 86 26.0 87 15.0 88 13.0 89 9.0 90 5.0 91 4.5 92 4.0 93 4.0 94 2.0 95 1.0 96 1.5 97 0.5 98 0.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20589016480047168 2 4.2807844342894726E-5 3 0.0 4 1.9458111064952148E-6 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.39657478870923646 14-15 0.0 16-17 2.62684499376854E-5 18-19 0.0 20-21 0.0 22-23 8.756149979228466E-6 24-25 1.6928556626508368E-4 26-27 6.323886096109448E-5 28-29 1.7512299958456932E-5 30-31 2.3057861611968294E-4 32-33 6.090388763330022E-4 34-35 9.729055532476074E-7 36-37 0.0 38-39 4.378074989614233E-5 40-41 2.9187166597428222E-5 42-43 2.0333726062874994E-4 44-45 1.7804171624431216E-4 46-47 6.421176651434209E-4 48-49 0.0028009950877998617 50-51 0.018293543117714764 52-53 0.046141991673874276 54-55 0.05927037920939749 56-57 0.05947274356447299 58-59 0.05973056353608361 60-61 0.0525145230476461 62-63 0.02547650481734185 64-65 0.011180630617921504 66-67 0.009252331811384747 68-69 0.00725009218280117 70-71 0.008613132862901069 72-73 0.010317663392190876 74-75 0.004410180872871405 76-77 0.005098025099017463 78-79 0.019700364547710802 80-81 0.0371241301008222 82-83 0.04696993429968799 84-85 0.047715179953475656 86-87 0.04245759834372558 88-89 0.025066911579424606 90-91 0.007841618759175715 92-93 0.0020353184173939947 94-95 0.00681909502271248 96-97 0.005027002993630387 98-99 0.0011023019918295393 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5.139245E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.003519909024064 #Duplication Level Percentage of deduplicated Percentage of total 1 65.24424641120744 15.00847321269482 2 15.161851666250461 6.9755191332452435 3 6.401088146160082 4.417426758288342 4 3.3153024842689773 3.050545068052734 5 2.030272564786354 2.3351707682404124 6 1.27897314265502 1.7652530490103095 7 0.8662755842188724 1.39491713537962 8 0.5929789972211669 1.091248333456819 9 0.4864182624227066 1.007039896537826 >10 3.7120188773589393 17.30999794969561 >50 0.48928634417693545 7.862453942900218 >100 0.36530450104795764 14.942489123192226 >500 0.030729947401342043 4.83542420270481 >1k 0.024832011517666015 10.143567247880464 >5k 2.9216365436005E-4 0.4274937498741567 >10k+ 1.28895651791214E-4 7.432980428846131 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3087246 6.007197555282926 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 241435 0.46978690449667215 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 122128 0.23763801881404759 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 83671 0.16280796109156112 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 83150 0.16179419350507712 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.04140102291289868 0.0 0.0 7.783244425980859E-6 0.0 2 0.0414360475128156 0.0 0.0 1.5566488851961718E-5 0.0 3 0.041445776568348074 0.0 0.0 1.7512299958456932E-5 0.0 4 0.04144772237945457 0.0 0.0 1.945811106495215E-5 0.0 5 0.04144966819056106 0.0 0.0 1.945811106495215E-5 1.9458111064952148E-6 6 0.04144966819056106 0.0 0.0 1.945811106495215E-5 1.9458111064952148E-6 7 0.04144966819056106 0.0 0.0 2.1403922171447363E-5 1.9458111064952148E-6 8 0.04144966819056106 0.0 0.0 2.1403922171447363E-5 1.9458111064952148E-6 9 0.04145161400166756 0.0 0.0 2.529554438443779E-5 1.9458111064952148E-6 10-11 0.04146037015164679 0.0 0.0 2.9187166597428222E-5 2.918716659742822E-6 12-13 0.04148274697937148 2.918716659742822E-6 0.0 3.697041102340908E-5 3.8916222129904295E-6 14-15 0.041495394751563705 3.8916222129904295E-6 0.0 4.572656100263755E-5 3.8916222129904295E-6 16-17 0.0415158257681819 3.8916222129904295E-6 0.0 5.740142764160884E-5 4.8645277662380365E-6 18-19 0.04154306712367284 3.8916222129904295E-6 0.0 6.907629428058013E-5 5.837433319485644E-6 20-21 0.04157420010137676 3.8916222129904295E-6 0.0 8.366987757929423E-5 6.810338872733252E-6 22-23 0.04166078869561579 3.8916222129904295E-6 0.0 8.561568868578945E-5 8.756149979228468E-6 24-25 0.04185342399515882 5.837433319485644E-6 0.0 8.950731089877987E-5 9.729055532476075E-6 26-27 0.04263855877662964 7.783244425980859E-6 0.0 9.437183866501792E-5 1.1674866638971289E-5 28-29 0.04574893783036224 7.783244425980859E-6 0.0 9.923636643125595E-5 1.3620677745466504E-5 30-31 0.054658806887003836 7.783244425980859E-6 0.0 9.923636643125595E-5 1.3620677745466504E-5 32-33 0.07472984845050197 7.783244425980859E-6 0.0 1.0020927198450355E-4 1.3620677745466504E-5 34-35 0.11263814042724174 7.783244425980859E-6 0.0 1.0312798864424639E-4 1.3620677745466504E-5 36-37 0.17599375005472595 7.783244425980859E-6 0.0 1.0993832751697963E-4 1.3620677745466504E-5 38-39 0.271937804093792 7.783244425980859E-6 0.0 1.1480285528321767E-4 1.5566488851961718E-5 40-41 0.40549438682141054 9.729055532476075E-6 0.0 1.2064028860270331E-4 1.7512299958456932E-5 42-43 0.5814054399041104 9.729055532476075E-6 0.0 1.2161319415595091E-4 1.7512299958456932E-5 44-45 0.8063733096982144 1.0701961085723682E-5 0.0 1.2550481636894136E-4 1.7512299958456932E-5 46-47 1.0840687688561257 1.1674866638971289E-5 0.0 1.2842353302868417E-4 1.848520551170454E-5 48-49 1.4182793386966372 1.1674866638971289E-5 0.0 1.303693441351794E-4 1.945811106495215E-5 50-51 1.8053556504895174 1.1674866638971289E-5 0.0 1.3328806079492223E-4 1.945811106495215E-5 52-53 2.240472092690658 1.1674866638971289E-5 0.0 1.3620677745466504E-4 1.945811106495215E-5 54-55 2.7281234889560624 1.1674866638971289E-5 0.0 1.430171163273983E-4 2.3349733277942577E-5 56-57 3.268997683511878 1.1674866638971289E-5 0.0 1.4982745520013155E-4 2.3349733277942577E-5 58-59 3.86173066277245 1.1674866638971289E-5 0.0 1.6150232183910284E-4 2.3349733277942577E-5 60-61 4.503546727194364 1.1674866638971289E-5 0.0 1.712313773715789E-4 2.3349733277942577E-5 62-63 5.18302299267694 1.3620677745466504E-5 0.0 1.8971658288328345E-4 2.724135549093301E-5 64-65 5.897791407103573 1.7512299958456932E-5 0.0 2.0431016618199755E-4 3.1132977703923436E-5 66-67 6.650473950940265 1.7512299958456932E-5 0.0 2.111205050547308E-4 3.1132977703923436E-5 68-69 7.439532460507332 1.7512299958456932E-5 0.0 2.1598503282096884E-4 3.1132977703923436E-5 70-71 8.258007742382393 1.945811106495215E-5 0.0 2.2668699390669252E-4 3.1132977703923436E-5 72-73 9.099162425609208 1.945811106495215E-5 0.0 2.2765989945994013E-4 3.210588325717105E-5 74-75 9.96108475077565 1.945811106495215E-5 0.0 2.364160494391686E-4 3.3078788810418653E-5 76-77 10.84433121985817 1.945811106495215E-5 0.0 2.3933476609891142E-4 3.599750547016147E-5 78-79 11.74559395397573 1.945811106495215E-5 0.0 2.4322638831190182E-4 3.697041102340908E-5 80-81 12.663706439369985 2.0431016618199758E-5 0.0 2.4711801052489227E-4 3.697041102340908E-5 82-83 13.594248766112532 2.1403922171447363E-5 0.0 2.607386882703588E-4 3.697041102340908E-5 84-85 14.537550749185922 2.3349733277942577E-5 0.0 2.636574049301016E-4 3.697041102340908E-5 86-87 15.487708797693045 2.3349733277942577E-5 0.0 2.7435936601582524E-4 3.697041102340908E-5 88 16.20552435231245 2.3349733277942577E-5 0.0 2.860342326547966E-4 3.697041102340908E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 398100 0.0 87.00722 8 TGGTATC 402600 0.0 85.96063 7 AGTGGTA 408255 0.0 85.73906 5 GTATCAA 402055 0.0 85.53793 9 GCAGTGG 414440 0.0 84.80284 3 AAGCAGT 397170 0.0 84.78678 1 GTGGTAT 411925 0.0 84.76883 6 AGCAGTG 425635 0.0 82.35612 2 CAGTGGT 441195 0.0 79.48671 4 ATCAACG 390495 0.0 45.870975 10-11 CAACGCA 391675 0.0 45.77329 12-13 ACGCAGA 403210 0.0 45.005043 14-15 TCAACGC 391580 0.0 43.830902 12-13 AGAGTAC 415495 0.0 43.50227 18-19 AACGCAG 398080 0.0 43.17612 14-15 TATCAAC 398130 0.0 43.135937 10-11 CGCAGAG 404540 0.0 42.459373 16-17 AGTACTT 433070 0.0 41.72116 20-21 GAGTACT 407900 0.0 41.614857 20-21 GCAGAGT 434945 0.0 41.597164 16-17 >>END_MODULE