FastQCFastQC Report
Thu 26 May 2016
SRR1660682_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660682_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26346810
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18331056.9575975231916125No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1439130.546225520281203No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT529410.20093893719960784No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT420540.1596170466177879No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2268750.087.041898
GCAGTGG2326950.086.162493
AGTGGTA2301100.086.114045
GTGGTAT2297450.086.056456
TGGTATC2290250.086.00057
AAGCAGT2339100.085.906481
GTATCAA2282600.085.7882849
AGCAGTG2409750.083.584282
CAGTGGT2522550.078.939834
ATCAACG2228950.045.6193910-11
CAACGCA2233550.045.52009212-13
ACGCAGA2270950.045.0299514-15
AGAGTAC2287350.044.53341718-19
TCAACGC2238600.043.72029512-13
AACGCAG2239950.043.4834214-15
TATCAAC2253100.043.33694510-11
CGCAGAG2278450.042.6904316-17
GAGTACT2283050.042.1070120-21
AGTACTT2452500.041.58146720-21
CAGAGTA2345950.041.1623918-19
Produced by FastQC (version 0.11.5)