FastQCFastQC Report
Thu 26 May 2016
SRR1660682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26346810
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18160246.892766145123451No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1440110.5465974818203797No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT544640.2067195231604889No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT426700.16195509057832808No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2235800.086.7854168
AAGCAGT2283300.086.189691
AGTGGTA2264900.086.039675
GCAGTGG2286000.085.997853
TGGTATC2253050.085.9395147
GTGGTAT2261050.085.924326
GTATCAA2248950.085.582199
AGCAGTG2363100.083.538022
CAGTGGT2483000.078.873924
ATCAACG2189300.045.57747310-11
CAACGCA2197350.045.4992912-13
ACGCAGA2234550.045.059114-15
AGAGTAC2263150.044.39528718-19
TCAACGC2197450.043.68387212-13
AACGCAG2202100.043.42162714-15
TATCAAC2219050.043.2607410-11
CGCAGAG2240750.042.6873416-17
GAGTACT2246050.042.03560620-21
AGTACTT2422800.041.3604320-21
CAGAGTA2326450.040.94223418-19
Produced by FastQC (version 0.11.5)