FastQCFastQC Report
Thu 26 May 2016
SRR1660681_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660681_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30227314
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22153807.329066684522482No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC10002113.3089642037000044Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1291270.42718648438296564No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT513530.16988939209087514No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT427660.14148131057890226No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2747500.087.968435
GGTATCA2737750.087.88538
GTGGTAT2772100.086.9094546
GCAGTGG2820150.086.843973
TGGTATC2767550.086.744467
GTATCAA2763000.086.438819
AAGCAGT2847700.085.737291
AGCAGTG2935750.083.8179552
CAGTGGT2918400.083.257874
CAACGCA2709450.045.72740612-13
ATCAACG2707250.045.6962210-11
ACGCAGA2748950.045.31691414-15
AGAGTAC2770300.044.71520218-19
TCAACGC2713400.044.026712-13
AACGCAG2725900.043.66465414-15
TATCAAC2739150.043.49815410-11
CGCAGAG2751150.043.17165416-17
AGTACTT2870300.043.16589720-21
GAGTACT2743950.042.74760420-21
GCAGAGT2920200.042.51729216-17
Produced by FastQC (version 0.11.5)