FastQCFastQC Report
Thu 26 May 2016
SRR1660681_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660681_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30227314
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17212405.694320044447218No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCAATCTCGTATG10402023.4412650756861827TruSeq Adapter, Index 16 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1365700.4518099094084244No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT501620.1659492470948626No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT425000.1406013117804645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2161550.086.367225
GGTATCA2153150.086.26778
GCAGTGG2212150.085.2030943
GTGGTAT2192150.084.951576
TGGTATC2186350.084.82237
GTATCAA2174950.084.748399
AAGCAGT2255800.083.660661
AGCAGTG2336500.081.112992
CAGTGGT2324850.080.7534264
CAACGCA2108900.045.42479712-13
ATCAACG2108600.045.3944910-11
ACGCAGA2146000.044.94384814-15
AGAGTAC2196200.043.9530218-19
TCAACGC2110450.043.49112712-13
AACGCAG2120600.043.14225414-15
TATCAAC2154000.042.72197710-11
CGCAGAG2148100.042.55166616-17
AGTACTT2269700.042.29791620-21
GAGTACT2148250.042.0097320-21
GCAGAGT2333700.041.38840516-17
Produced by FastQC (version 0.11.5)