FastQCFastQC Report
Thu 26 May 2016
SRR1660680_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660680_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36311154
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20634285.6826285388781645No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1416100.38999035943611154No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580290.15981039875515937No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT397580.10949252673159326No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2600750.086.4240345
GGTATCA2594150.086.2429058
GCAGTGG2669150.085.1786353
TGGTATC2630200.084.932497
GTATCAA2618100.084.803549
GTGGTAT2648150.084.683516
AAGCAGT2765450.082.456831
AGCAGTG2830000.080.755112
CAGTGGT2814950.080.261764
CAACGCA2536750.045.3763312-13
ATCAACG2540000.045.311910-11
ACGCAGA2576950.044.9324114-15
AGAGTAC2617700.044.0440718-19
TCAACGC2544100.043.6001612-13
AACGCAG2551950.043.26373714-15
TATCAAC2581850.042.88915310-11
CGCAGAG2586100.042.63778316-17
GAGTACT2575500.042.23731620-21
AGTACTT2738900.042.03959320-21
GCAGAGT2828800.040.840916-17
Produced by FastQC (version 0.11.5)