FastQCFastQC Report
Thu 26 May 2016
SRR1660680_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660680_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36311154
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19912555.483865921749554No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1498350.41264180146959806No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT552610.1521873967431605No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT386410.10641633697458364No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2493600.086.286418
AGTGGTA2504950.086.2349555
GCAGTGG2563850.084.92833
GTATCAA2520250.084.741949
TGGTATC2536850.084.687537
GTGGTAT2551150.084.597786
AAGCAGT2658100.082.084821
AGCAGTG2727700.080.24192
CAGTGGT2708750.080.1254
CAACGCA2434600.045.33323312-13
ATCAACG2432150.045.3209410-11
ACGCAGA2478850.044.85571314-15
AGAGTAC2540700.043.80194518-19
TCAACGC2438300.043.63701612-13
AACGCAG2452550.043.23594714-15
TATCAAC2481000.042.9636410-11
CGCAGAG2485900.042.666316-17
GAGTACT2480600.042.18119420-21
AGTACTT2638550.041.93384620-21
GCAGAGT2736100.040.72678816-17
Produced by FastQC (version 0.11.5)