FastQCFastQC Report
Thu 26 May 2016
SRR1660679_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660679_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28347502
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6463842.280215025648468No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC622880.21973011942992365Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA476030.16792661307511328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA921200.078.17538
GTATCAA928800.077.313029
GTGGTAT933750.077.230446
TGGTATC940000.076.493837
AGTGGTA949450.076.116135
AAGCAGT1029400.071.103891
CAGTGGT1059600.068.621684
GCAGTGG1071600.068.406043
AGCAGTG1134000.064.794122
CAACGCA892950.042.8946712-13
ATCAACG914300.041.97802710-11
ACGCAGA930100.041.31500214-15
TCAACGC885900.040.5228312-13
AGAGTAC935350.040.44442718-19
AACGCAG913950.039.2737614-15
GAGTACT876650.039.15021520-21
AGTACTT952800.038.39092620-21
TATCAAC948700.037.84815610-11
CGCAGAG952400.037.66864416-17
GTACTTT936100.036.60558322-23
Produced by FastQC (version 0.11.5)