##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660679_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28347502
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09728003546838	34.0	31.0	34.0	30.0	34.0
2	32.28074131540762	34.0	31.0	34.0	30.0	34.0
3	32.31312565036595	34.0	31.0	34.0	30.0	34.0
4	35.66626279804125	37.0	35.0	37.0	35.0	37.0
5	35.66616917427151	37.0	35.0	37.0	35.0	37.0
6	35.67640647842621	37.0	36.0	37.0	35.0	37.0
7	35.63843272680605	37.0	35.0	37.0	35.0	37.0
8	35.64921992068296	37.0	36.0	37.0	35.0	37.0
9	37.34009134208721	39.0	38.0	39.0	35.0	39.0
10-11	37.29800542919091	39.0	38.0	39.0	34.5	39.0
12-13	37.32567155299962	39.0	38.0	39.0	35.0	39.0
14-15	38.76372032710325	41.0	39.0	41.0	35.0	41.0
16-17	38.73708020198746	41.0	39.0	41.0	35.0	41.0
18-19	38.7195358342333	41.0	39.0	41.0	35.0	41.0
20-21	38.68146147410096	40.5	39.0	41.0	34.5	41.0
22-23	38.59945327810542	40.0	38.5	41.0	34.0	41.0
24-25	38.594438762187934	40.0	38.5	41.0	34.0	41.0
26-27	38.55278514487802	40.0	38.0	41.0	34.0	41.0
28-29	38.461136011208325	40.0	38.0	41.0	34.0	41.0
30-31	38.369537569835956	40.0	38.0	41.0	34.0	41.0
32-33	38.27498518211587	40.0	38.0	41.0	33.0	41.0
34-35	38.176620783023495	40.0	38.0	41.0	33.0	41.0
36-37	38.04489381462959	40.0	38.0	41.0	33.0	41.0
38-39	37.81306585673757	40.0	37.0	41.0	33.0	41.0
40-41	37.68397848600557	40.0	37.0	41.0	32.5	41.0
42-43	37.51065159109963	40.0	37.0	41.0	32.0	41.0
44-45	37.36863329262663	40.0	36.5	41.0	31.5	41.0
46-47	37.18442810234214	40.0	36.0	41.0	31.0	41.0
48-49	37.11118889770252	39.5	36.0	41.0	31.0	41.0
50-51	36.398648071353875	38.5	35.0	40.0	30.0	40.5
52-53	36.50236200706503	39.0	35.0	40.0	30.5	40.5
54-55	36.81515094345879	39.0	35.0	41.0	30.0	41.0
56-57	36.576263880323566	39.0	35.0	41.0	30.0	41.0
58-59	36.31393274088136	39.0	35.0	41.0	29.0	41.0
60-61	36.10761038133096	39.0	35.0	41.0	29.0	41.0
62-63	35.92035176503383	38.0	35.0	41.0	29.0	41.0
64-65	35.62359990308846	37.5	35.0	40.0	29.0	41.0
66-67	35.28129265146538	37.0	35.0	40.0	28.0	41.0
68-69	34.93677130704498	36.0	34.0	39.0	28.0	41.0
70-71	34.59299161527531	36.0	34.0	39.0	28.0	41.0
72-73	34.235112797593246	35.0	34.0	38.5	27.0	40.5
74-75	33.88579882629517	35.0	34.0	37.0	27.0	40.0
76-77	33.544945794518334	35.0	33.5	37.0	26.0	39.0
78-79	33.22205065899634	35.0	33.0	36.5	26.0	39.0
80-81	32.92170544692086	35.0	33.0	36.0	26.0	38.0
82-83	32.66048444056905	35.0	33.0	35.5	25.5	37.0
84-85	32.431742909833815	35.0	33.0	35.0	25.0	37.0
86-87	32.22125568595074	35.0	33.0	35.0	25.0	36.0
88-89	32.05274600562688	35.0	33.0	35.0	24.5	36.0
90-91	31.900153618474036	35.0	32.5	35.0	24.0	36.0
92-93	31.76583721556841	35.0	32.0	35.0	24.0	35.5
94-95	31.651945822245644	35.0	32.0	35.0	24.0	35.0
96-97	31.618585845765175	35.0	32.0	35.0	24.0	35.0
98-99	31.439979067644124	35.0	32.0	35.0	23.0	35.0
100	31.376350868588	35.0	32.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.16594313943197392
1101	2	0.217876302186923
1101	3	0.22581879176073016
1101	4	0.18953349573048683
1101	5	0.18359595461433997
1101	6	0.16543286781517708
1101	7	0.1715833578629642
1101	8	0.1521162706802528
1101	9	0.22441558963984676
1101	10-11	0.21561689233047332
1101	12-13	0.2337113570786613
1101	14-15	0.2694739168635465
1101	16-17	0.29433485312208774
1101	18-19	0.29471056177815314
1101	20-21	0.2702444390196561
1101	22-23	0.2748856237056998
1101	24-25	0.33359445476766325
1101	26-27	0.3459764786194981
1101	28-29	0.3564111143312019
1101	30-31	0.3865612351786325
1101	32-33	0.38935203975319155
1101	34-35	0.36273331093855177
1101	36-37	0.4524740173698092
1101	38-39	0.45239113327395586
1101	40-41	0.4282218792195991
1101	42-43	0.4307960483574007
1101	44-45	0.4571279173484868
1101	46-47	0.4517883338345001
1101	48-49	0.4707464689183851
1101	50-51	0.5829910285498627
1101	52-53	0.55857414297747
1101	54-55	0.4669069846547771
1101	56-57	0.4551120005279117
1101	58-59	0.4704953887205221
1101	60-61	0.44656704122751023
1101	62-63	0.3973594688044386
1101	64-65	0.3670103721452378
1101	66-67	0.34999420129013004
1101	68-69	0.35264500124976905
1101	70-71	0.3639361313313074
1101	72-73	0.37329379527952256
1101	74-75	0.3800814465548896
1101	76-77	0.36947012578685445
1101	78-79	0.3618305734852356
1101	80-81	0.40132855040167925
1101	82-83	0.3771212526665906
1101	84-85	0.3739007110193313
1101	86-87	0.36825319629197395
1101	88-89	0.3729588043719616
1101	90-91	0.3610841367061681
1101	92-93	0.3671152992597335
1101	94-95	0.3500401843472041
1101	96-97	0.38920366679269236
1101	98-99	0.2866396684275223
1101	100	0.301848940862385
1102	1	0.06446498495353836
1102	2	0.14767623519514217
1102	3	0.19771391075901334
1102	4	0.14359901580795054
1102	5	0.15288543204793825
1102	6	0.14608296976528834
1102	7	0.14384429682653632
1102	8	0.1561538727010685
1102	9	0.2008214649120461
1102	10-11	0.19953882056262273
1102	12-13	0.19254810134835054
1102	14-15	0.25097600950117993
1102	16-17	0.24829659957629246
1102	18-19	0.2649731168118237
1102	20-21	0.22674954488442012
1102	22-23	0.25331096098087613
1102	24-25	0.2852186205158205
1102	26-27	0.3109269178385219
1102	28-29	0.3069333796692604
1102	30-31	0.3362977063562482
1102	32-33	0.3618674586466142
1102	34-35	0.3349014095528986
1102	36-37	0.3667614008644122
1102	38-39	0.3859215889864629
1102	40-41	0.39127781424449637
1102	42-43	0.3825008994129959
1102	44-45	0.3816878357887532
1102	46-47	0.39839866827254156
1102	48-49	0.40349108183554705
1102	50-51	0.4891820842424224
1102	52-53	0.45734901306619946
1102	54-55	0.38255996627761135
1102	56-57	0.38429484133403946
1102	58-59	0.4094697350490435
1102	60-61	0.38665872881158236
1102	62-63	0.34746363976013583
1102	64-65	0.32826932701396316
1102	66-67	0.27612385402974127
1102	68-69	0.3234380665643215
1102	70-71	0.32824628613058593
1102	72-73	0.30054810514342023
1102	74-75	0.3016219398692286
1102	76-77	0.2829083460504549
1102	78-79	0.3074943223454838
1102	80-81	0.31303305273026893
1102	82-83	0.2641884896652513
1102	84-85	0.32526369258966525
1102	86-87	0.3266367008283737
1102	88-89	0.32744887179941884
1102	90-91	0.35326832619358584
1102	92-93	0.37588408052363675
1102	94-95	0.33468271459718224
1102	96-97	0.36649763901115406
1102	98-99	0.28363077570610074
1102	100	0.2989782994905177
1103	1	-0.0032685451901031115
1103	2	0.060703743169923996
1103	3	0.09116743278834605
1103	4	0.08683468420372975
1103	5	0.06321538857874032
1103	6	0.08519965074555813
1103	7	0.10699707551010817
1103	8	0.12984075442430765
1103	9	0.12378828464835578
1103	10-11	0.1081101047268831
1103	12-13	0.09730633188632254
1103	14-15	0.1391819336271567
1103	16-17	0.13280560701770838
1103	18-19	0.16890356446843668
1103	20-21	0.1794650827614035
1103	22-23	0.20637397894464016
1103	24-25	0.18750283276654045
1103	26-27	0.22313083438748293
1103	28-29	0.21596947752571793
1103	30-31	0.24386594827145558
1103	32-33	0.2518191139070467
1103	34-35	0.24691886028155352
1103	36-37	0.2655958899743851
1103	38-39	0.30194105830227613
1103	40-41	0.3091933661935329
1103	42-43	0.3225292543416387
1103	44-45	0.35366337984239493
1103	46-47	0.3348745387417864
1103	48-49	0.35829112988698597
1103	50-51	0.4552141652378978
1103	52-53	0.45017173544827216
1103	54-55	0.3814454338175395
1103	56-57	0.381763229343008
1103	58-59	0.3805240499444409
1103	60-61	0.35351288086436483
1103	62-63	0.2672592234554614
1103	64-65	0.2383646239914583
1103	66-67	0.25878119406531397
1103	68-69	0.2247415561885049
1103	70-71	0.2493452690911937
1103	72-73	0.23904104658333836
1103	74-75	0.28872773410309804
1103	76-77	0.308112159874284
1103	78-79	0.2672686194056695
1103	80-81	0.2511274592173365
1103	82-83	0.28267931119351886
1103	84-85	0.2716536147049524
1103	86-87	0.31136278278408724
1103	88-89	0.31367665529950983
1103	90-91	0.30883279660355356
1103	92-93	0.34219772973201046
1103	94-95	0.31214338280966913
1103	96-97	0.3749109685809877
1103	98-99	0.3033500264594693
1103	100	0.30930938181800727
1104	1	-0.05299334348953266
1104	2	0.015181230034208681
1104	3	0.04041099102237666
1104	4	0.005953244148429349
1104	5	0.032458748577234076
1104	6	0.01569771506932227
1104	7	0.030634859830108496
1104	8	0.028633242664930947
1104	9	0.05309241011849508
1104	10-11	0.039053824257806014
1104	12-13	0.031893083090061225
1104	14-15	0.062307838204908705
1104	16-17	0.06422416215171012
1104	18-19	0.03334811264856796
1104	20-21	0.11014738587405759
1104	22-23	0.14699668094193896
1104	24-25	0.14143976219769883
1104	26-27	0.14339309728270422
1104	28-29	0.17706258372233918
1104	30-31	0.19545170595114314
1104	32-33	0.2202514409174512
1104	34-35	0.20312974727443134
1104	36-37	0.19622269967256756
1104	38-39	0.2383062343796567
1104	40-41	0.20892847732381625
1104	42-43	0.24297523961057976
1104	44-45	0.2937121500142581
1104	46-47	0.29865519687673014
1104	48-49	0.3214678727782214
1104	50-51	0.4234887949802584
1104	52-53	0.35887958329207237
1104	54-55	0.28960723625596785
1104	56-57	0.2836822518092532
1104	58-59	0.3317114563091792
1104	60-61	0.28395926710912534
1104	62-63	0.23916546497879665
1104	64-65	0.25704698467624354
1104	66-67	0.22505812356183696
1104	68-69	0.2233504198544125
1104	70-71	0.208358296047507
1104	72-73	0.2529716034641467
1104	74-75	0.2640673113961327
1104	76-77	0.24781368424874017
1104	78-79	0.2541062905044882
1104	80-81	0.24940534971355532
1104	82-83	0.26136558336752813
1104	84-85	0.2578569836720135
1104	86-87	0.25626842668513206
1104	88-89	0.23235401387320564
1104	90-91	0.23033162447622146
1104	92-93	0.2740567915900449
1104	94-95	0.2681951016325961
1104	96-97	0.3360322993810705
1104	98-99	0.2701167310391064
1104	100	0.2927411217537852
1105	1	-0.0954565086902548
1105	2	0.0026811313453123375
1105	3	0.02772593558376002
1105	4	0.03838982819827663
1105	5	0.031500128114004156
1105	6	0.03702601948440787
1105	7	0.032500644938643575
1105	8	0.050939465758027325
1105	9	0.06611920066759325
1105	10-11	0.00790940539478413
1105	12-13	0.05490570741743994
1105	14-15	0.06324813821009201
1105	16-17	0.05444359518831021
1105	18-19	0.054579306216211876
1105	20-21	0.08890476545951742
1105	22-23	0.107466405431623
1105	24-25	0.13031813855791086
1105	26-27	0.14690477573332572
1105	28-29	0.1461226998786742
1105	30-31	0.13348618364072706
1105	32-33	0.1769919348657396
1105	34-35	0.17702557000495744
1105	36-37	0.17425987923398623
1105	38-39	0.2329629898709058
1105	40-41	0.25466801218893664
1105	42-43	0.23896802296712139
1105	44-45	0.20913028282288337
1105	46-47	0.20783060194892755
1105	48-49	0.19505228680792897
1105	50-51	0.2946420260249951
1105	52-53	0.29932675304042533
1105	54-55	0.24472376759258196
1105	56-57	0.25693330850857876
1105	58-59	0.2900461552143412
1105	60-61	0.2610923181851561
1105	62-63	0.24428908072914624
1105	64-65	0.23585608430528993
1105	66-67	0.2247497271553982
1105	68-69	0.26866715468716507
1105	70-71	0.24599031408884287
1105	72-73	0.22014287625633244
1105	74-75	0.20472346173023936
1105	76-77	0.20139403738564
1105	78-79	0.2344532547505267
1105	80-81	0.24697053636531763
1105	82-83	0.25731707628857947
1105	84-85	0.2269174103239422
1105	86-87	0.2678166262273223
1105	88-89	0.23866329299305988
1105	90-91	0.2530774588075815
1105	92-93	0.2789076759197435
1105	94-95	0.2686108631558817
1105	96-97	0.3038836933034581
1105	98-99	0.2674811390594698
1105	100	0.2541082643709984
1106	1	-0.07543439520736683
1106	2	-0.013447406195567169
1106	3	0.015869379155162733
1106	4	-0.0028431285259031824
1106	5	-0.0037345253131491063
1106	6	-0.014298723287268444
1106	7	-0.012895052578130617
1106	8	0.015883214526411393
1106	9	0.014591199005550948
1106	10-11	0.0020499295776090776
1106	12-13	-0.001003261522832588
1106	14-15	0.032030785518792015
1106	16-17	0.042651646475086125
1106	18-19	0.0368311223760216
1106	20-21	0.09350797760025387
1106	22-23	0.10438416153407104
1106	24-25	0.07764288457175894
1106	26-27	0.031425185222921925
1106	28-29	0.08834750054793972
1106	30-31	0.1279977837491515
1106	32-33	0.15971612668337087
1106	34-35	0.143056116849678
1106	36-37	0.1480363432337768
1106	38-39	0.18166743127687113
1106	40-41	0.13396262901282086
1106	42-43	0.21263844074166371
1106	44-45	0.15446162540422392
1106	46-47	0.19821637080057286
1106	48-49	0.1813383083625908
1106	50-51	0.27473429088899337
1106	52-53	0.21568166693056412
1106	54-55	0.21913246995990932
1106	56-57	0.2362393498870432
1106	58-59	0.23319674641351185
1106	60-61	0.20327397453785068
1106	62-63	0.16728618156295738
1106	64-65	0.13953000134196003
1106	66-67	0.14660261635143712
1106	68-69	0.1597018476725367
1106	70-71	0.13526038323222167
1106	72-73	0.16089888118136741
1106	74-75	0.1501271007503604
1106	76-77	0.130302097343872
1106	78-79	0.16588420970298046
1106	80-81	0.17366858291484988
1106	82-83	0.20504815690929945
1106	84-85	0.17526087326415052
1106	86-87	0.1874324403643115
1106	88-89	0.22006036947939833
1106	90-91	0.23390078434466943
1106	92-93	0.2454482956057582
1106	94-95	0.22114382630213925
1106	96-97	0.2770322693152458
1106	98-99	0.248336838289287
1106	100	0.25704104296591623
1107	1	-0.08575585123374196
1107	2	-0.01507441032946133
1107	3	8.259453319823251E-4
1107	4	-0.01974912251875338
1107	5	-0.015396004605008784
1107	6	-0.009784926347535361
1107	7	0.0012296419428352579
1107	8	0.0021972798859195564
1107	9	-0.0074613017379618896
1107	10-11	0.014618322532591321
1107	12-13	0.0037298434132750913
1107	14-15	-0.0012256905739889135
1107	16-17	8.758439062717116E-5
1107	18-19	0.03165678623387436
1107	20-21	0.056147660593147464
1107	22-23	0.0809331836691527
1107	24-25	0.06303987124385912
1107	26-27	0.1269038098020232
1107	28-29	0.09714364509795814
1107	30-31	0.10863570394976563
1107	32-33	0.11021237304319698
1107	34-35	0.12687586038434517
1107	36-37	0.17696921137105193
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2316	54-55	-0.44280119854057176
2316	56-57	-0.4688905613890313
2316	58-59	-0.41019885990270666
2316	60-61	-0.433563387952546
2316	62-63	-0.4866973452498655
2316	64-65	-0.4975353428095346
2316	66-67	-0.4639064626653777
2316	68-69	-0.4614614302467288
2316	70-71	-0.4207366135033084
2316	72-73	-0.34914758753728137
2316	74-75	-0.40048918714663984
2316	76-77	-0.3432346354451923
2316	78-79	-0.39396367862528336
2316	80-81	-0.3155693460050486
2316	82-83	-0.3195619985573188
2316	84-85	-0.37974532174357734
2316	86-87	-0.3468029535384147
2316	88-89	-0.33250618652941455
2316	90-91	-0.3477865457892051
2316	92-93	-0.3258603383576997
2316	94-95	-0.33593998974048134
2316	96-97	-0.3668758523578113
2316	98-99	-0.4056929121226922
2316	100	-0.37357735523627866
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	787.0
3	591.0
4	248.0
5	209.0
6	606.0
7	6644.0
8	16899.0
9	22931.0
10	24409.0
11	25024.0
12	26888.0
13	29820.0
14	35981.0
15	46222.0
16	59047.0
17	69998.0
18	76747.0
19	85473.0
20	96990.0
21	112962.0
22	140245.0
23	191100.0
24	264285.0
25	337156.0
26	360785.0
27	342449.0
28	316209.0
29	341479.0
30	397676.0
31	486482.0
32	622049.0
33	828082.0
34	1181944.0
35	1894022.0
36	3476013.0
37	6799982.0
38	7637141.0
39	1988387.0
40	3540.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.74988548315325	26.98000644582668	29.46477893165815	26.805329139361923
2	23.516149762966943	31.237195044698012	24.87977184972602	20.366883342609025
3	24.130333341742634	24.700448650898227	27.130026196442092	24.03919181091705
4	20.269705585608925	27.982975437142553	23.404774917977036	28.342544059271486
5	23.925068243276773	28.73355894856048	26.0502963414573	21.291076466705448
6	28.26074750447816	28.449776549902833	23.949937353227043	19.339538592391964
7	23.53249551529324	25.3410993562749	28.307246070185172	22.819159058246687
8	26.072865682578154	27.549256030371126	24.237262951389745	22.14061533566097
9	24.415044236610388	25.360161797183256	27.159039199746605	23.065754766459754
10-11	22.808487958324942	28.32695530343397	26.473741566996022	22.390815171245066
12-13	22.499588194051785	26.922814494059395	26.702564902787163	23.875032409101664
14-15	22.468510803951684	29.40701095244785	25.74592775105819	22.378550492542274
16-17	23.912729096802117	26.459956508811068	25.75452830942221	23.872786084964606
18-19	22.393832521795964	27.762967876174145	26.04800249789747	23.795197104132424
20-21	23.62577307155573	28.135179270889804	25.96166160629252	22.277386051261946
22-23	23.92698824122486	26.598866886896115	27.041076254060638	22.433068617818382
24-25	22.627156504625727	27.72700666561508	25.67315628003126	23.972680549727933
26-27	22.561955525440457	26.6070171812173	28.425156246325905	22.405871047016333
28-29	22.722284723419587	26.51451444331579	28.358933068750225	22.404267764514397
30-31	22.540147644919113	26.270247848406868	28.635630045991718	22.553974460682298
32-33	22.7561482522153	26.5264153388133	28.301212384593338	22.416224024378057
34-35	22.801493695686332	26.794567908482403	27.99010271195317	22.41383568387809
36-37	22.522398548265617	26.725946890880557	28.335894723961026	22.4157598368928
38-39	22.609073162344735	26.609483430255366	28.37590059901479	22.40554280838511
40-41	22.797460509157442	26.63727895595973	28.15070448135802	22.4145560535248
42-43	22.799664570334162	26.656924023169594	28.248320271646165	22.295091134850082
44-45	23.028703228851057	26.776394840783208	28.046176528922796	22.148725401442938
46-47	22.868080209241377	26.798400286244583	28.04227710587911	22.291242398634925
48-49	23.020244649412135	26.811410118277756	27.881160749074006	22.287184483236093
50-51	23.152692457314718	26.876529779231955	27.813864108643987	22.156913654809337
52-53	23.1946963958028	27.034073154002048	27.744689036683628	22.026541413511527
54-55	23.368347758611797	27.05309150876385	27.394901107860893	22.183659624763457
56-57	23.74916272589685	27.469689782127105	26.849607232065274	21.93154025991077
58-59	24.011109714062982	27.64993572059436	26.480011901979722	21.85894266336294
60-61	24.082611373802585	27.888225062396625	26.28160503968693	21.747558524113863
62-63	24.24434882635588	28.00778895620898	26.163633322388506	21.584228895046635
64-65	24.442412468297224	28.02667584119834	26.053067110074917	21.477844580429515
66-67	24.512405653740792	27.92530012397935	26.185194545261286	21.377099677018567
68-69	24.707124225058163	27.887043615369855	26.21855058803803	21.18728157153395
70-71	24.897653744771404	27.73472145634709	26.239196455815595	21.128428343065913
72-73	25.038725175958888	27.68506374833025	26.247182508783034	21.029028566927824
74-75	25.312770895772076	27.718642502386658	26.148561021243548	20.82002558059772
76-77	25.564703644237508	27.69408386650296	25.954801123879363	20.78641136538017
78-79	25.759914159438637	27.68729688246593	25.848827966472086	20.703960991623347
80-81	26.00491134786153	27.736877716838837	25.655566981448114	20.60264395385152
82-83	26.1842951927174	27.696333027803778	25.504117591533156	20.615254187945666
84-85	26.295640335028615	27.650121179980967	25.4666212254604	20.587617259530013
86-87	26.44682570490878	27.669306913222997	25.399123054663512	20.484744327204716
88-89	26.576508564884392	27.599782934528612	25.337986131065588	20.48572236952141
90-91	26.639100953498453	27.577588166791255	25.349948661467852	20.43336221824244
92-93	26.716135770773068	27.680689433365767	25.3194970312115	20.28367776464966
94-95	26.758490555517778	27.740598631463786	25.217580757395698	20.28333005562274
96-97	26.710558938441054	27.85604359145496	25.217334092068295	20.216063378035695
98-99	26.758331532926256	28.051145503517127	25.12066852419295	20.069854439363667
100	26.708654520986492	28.15169496827942	25.016680548291458	20.122969962442635
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	47061.0
1	32556.5
2	14958.5
3	10915.5
4	9352.0
5	8388.0
6	8052.5
7	8052.5
8	7818.0
9	7660.0
10	8001.5
11	10757.5
12	70062.5
13	81460.5
14	34791.0
15	35745.0
16	39981.0
17	44177.0
18	48693.5
19	53323.5
20	59262.0
21	69061.0
22	83958.5
23	101442.5
24	120213.0
25	141874.0
26	164876.0
27	192552.5
28	229388.0
29	267628.0
30	302146.0
31	340972.5
32	382164.0
33	417673.5
34	451728.0
35	494378.0
36	543851.0
37	587154.5
38	631994.0
39	679014.5
40	716439.5
41	757701.5
42	809203.5
43	844813.0
44	871989.0
45	912585.5
46	960286.5
47	1010376.0
48	1056199.5
49	1090450.0
50	1112728.5
51	1126573.5
52	1127412.5
53	1113051.5
54	1082177.5
55	1034907.5
56	975839.0
57	902422.5
58	809703.0
59	698292.0
60	577838.0
61	465271.5
62	366827.5
63	279353.5
64	207358.0
65	152565.0
66	111071.0
67	82111.5
68	61524.0
69	46512.0
70	35920.5
71	28330.0
72	23182.5
73	18363.0
74	14465.5
75	12120.0
76	9313.5
77	5876.0
78	3721.0
79	2677.0
80	1973.5
81	1502.5
82	1148.5
83	930.5
84	782.0
85	649.5
86	549.0
87	464.5
88	403.5
89	348.0
90	303.5
91	257.0
92	200.5
93	173.5
94	143.5
95	118.5
96	113.0
97	93.5
98	69.0
99	52.0
100	43.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.23154421154992777
2	0.04159802158228969
3	0.020696709008081206
4	0.0546679562805922
5	0.05047358317498311
6	0.0410794573715878
7	0.025748300502809737
8	0.0252755957121019
9	0.010159625352526652
10-11	0.010812240175518817
12-13	0.010022047092544522
14-15	0.01176823270001004
16-17	0.011888172721532924
18-19	0.01292706496678261
20-21	0.013221623549052047
22-23	0.009420583161084177
24-25	0.01086868253858841
26-27	0.010284856845587312
28-29	0.01426933491353136
30-31	0.01420760107892399
32-33	0.014038273989715213
34-35	0.012614868146053929
36-37	0.011283181142380729
38-39	0.017802274076918666
40-41	0.020589115753479797
42-43	0.016754562712439355
44-45	0.015526941315675715
46-47	0.018719462476799544
48-49	0.018163857965333242
50-51	0.01538054393646396
52-53	0.018045681767656283
54-55	0.02113942879340832
56-57	0.022137753090201738
58-59	0.02116059467955942
60-61	0.01913396108059186
62-63	0.024208482285317415
64-65	0.023732249846917727
66-67	0.021481610619517727
68-69	0.02465120207064453
70-71	0.026649614488077292
72-73	0.032976450623409426
74-75	0.03255136907654156
76-77	0.03381250312637777
78-79	0.033101682116470084
80-81	0.031203807658255034
82-83	0.03078578140677087
84-85	0.037109089894411154
86-87	0.03704735605980379
88-89	0.036791601602144695
90-91	0.04254343116370536
92-93	0.034147629657103475
94-95	0.033981830215586546
96-97	0.03211570458659814
98-99	0.22769731174196584
100	0.09556397597220383
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.8347502E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.56720031715487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.7693042307583	37.284701767838435
2	11.25641032743528	10.933846704492801
3	4.215153591826393	6.1415462658542195
4	2.151245745294898	4.179199321726578
5	1.3339843596907481	3.2393942808526073
6	0.8789701274265381	2.561347095091189
7	0.5839208035008596	1.9851579043086192
8	0.44946798407134597	1.7463521294832667
9	0.31023876364730835	1.3560685362184763
>10	1.8157937690010764	16.254980442282633
>50	0.15746797861190637	5.311265927004311
>100	0.07681579239382892	5.674654285160322
>500	9.54703260772688E-4	0.3212651435051803
>1k	2.353097518723353E-4	0.1972257698925998
>5k	0.0	0.0
>10k+	3.651332894128369E-5	2.812994426288708
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	646384	2.280215025648468	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	62288	0.21973011942992365	Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47603	0.16792661307511328	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.253341546637866	3.527647691849532E-5	0.0	0.0	0.0
2	0.25335918487632525	5.291471537774299E-5	0.0	0.0	3.5276476918495323E-6
3	0.2533697678194008	5.644236306959252E-5	0.0	0.0	7.055295383699065E-6
4	0.2533697678194008	8.819119229623831E-5	0.0	0.0	1.0582943075548597E-5
5	0.2534015166486275	1.1288472613918503E-4	0.0	0.0	2.1165886151097195E-5
6	0.2534403207732378	1.199400215228841E-4	0.0	0.0	2.4693533842946725E-5
7	0.25344384842092965	1.2699531690658317E-4	0.0	3.5276476918495323E-6	3.880412461034485E-5
8	0.25345090371631335	1.3757825998213176E-4	0.0	3.5276476918495323E-6	3.880412461034485E-5
9	0.2534685419547726	1.4816120305768035E-4	0.0	7.055295383699065E-6	5.291471537774299E-5
10-11	0.2535302757893799	1.569803222873042E-4	0.0	1.0582943075548597E-5	5.644236306959252E-5
12-13	0.25360082874321693	1.7814620843840137E-4	0.0	1.763823845924766E-5	6.173383460736682E-5
14-15	0.25368725611166726	1.9402062305172427E-4	0.0	1.940206230517243E-5	6.702530614514111E-5
16-17	0.2537666281847339	2.1165886151097192E-4	0.0	2.1165886151097195E-5	8.2899720758464E-5
18-19	0.2538495279054923	2.3635239535391866E-4	0.0	2.822118153479626E-5	1.0053795921771168E-4
20-21	0.2540558952954656	2.7339269611833877E-4	0.0	3.7040300764420094E-5	1.305229645984327E-4
22-23	0.25470674629461176	3.0337770149905976E-4	0.0	4.233177230219439E-5	1.3757825998213176E-4
24-25	0.2573824670688797	3.227797638042322E-4	0.0	4.233177230219439E-5	1.4816120305768035E-4
26-27	0.2664732151707759	3.3689035457163037E-4	0.0	4.233177230219439E-5	1.4816120305768035E-4
28-29	0.29098507515759237	3.756944791819752E-4	0.0	4.233177230219439E-5	1.5874414613322894E-4
30-31	0.3434200304492438	4.0744330840862097E-4	0.0	4.233177230219439E-5	1.622717938250785E-4
32-33	0.4352782125211597	4.1979007533009434E-4	0.0	4.233177230219439E-5	1.728547369006271E-4
34-35	0.5760613404313368	4.37428313789342E-4	0.0	4.233177230219439E-5	1.8343767997617568E-4
36-37	0.7699390937515411	4.515389045567401E-4	0.0	4.233177230219439E-5	1.9402062305172427E-4
38-39	1.0155321622342597	4.638856714782135E-4	0.0	4.585941999404392E-5	2.028397422813481E-4
40-41	1.3172183566650775	4.7799626224561163E-4	0.0	4.585941999404392E-5	2.063673899731976E-4
42-43	1.6730662899327071	5.150365630100317E-4	0.0	4.585941999404392E-5	2.1342268535689673E-4
44-45	2.0818853809411495	5.43257744544828E-4	0.0	4.585941999404392E-5	2.345885715079939E-4
46-47	2.5489053673935715	5.87353340692947E-4	0.0	4.585941999404392E-5	2.451715145835425E-4
48-49	3.0717539062172037	6.138106983818186E-4	0.0	4.585941999404392E-5	2.4869916227539203E-4
50-51	3.6436914264967686	6.49087175300314E-4	0.0	4.585941999404392E-5	2.610459291968654E-4
52-53	4.261243195255794	6.755445329891855E-4	0.0	4.938706768589345E-5	2.751565199642635E-4
54-55	4.91778252630514	7.02001890678057E-4	0.0	5.291471537774299E-5	2.786841676561131E-4
56-57	5.613328821707112	7.549166060558E-4	0.0	5.644236306959252E-5	2.786841676561131E-4
58-59	6.354552863246997	7.796101398987467E-4	0.0	5.644236306959252E-5	2.875032868857369E-4
60-61	7.1334874586127555	7.9195690682022E-4	0.0	5.644236306959252E-5	2.9985005380721026E-4
62-63	7.941433428596284	8.113589691253924E-4	0.0	5.644236306959252E-5	3.1219682072868363E-4
64-65	8.773661961466658	8.431077983520382E-4	0.0	6.526148229921635E-5	3.24543587650157E-4
66-67	9.634464440641015	8.536907414275868E-4	0.0	6.878912999106588E-5	3.333627068797808E-4
68-69	10.518247780703923	8.572183891194363E-4	0.0	7.408060152884018E-5	3.4041800226347987E-4
70-71	11.42232215029035	8.713289798868344E-4	0.0	7.584442537476494E-5	3.421818261094046E-4
72-73	12.345754486585802	8.960225137297812E-4	0.0	7.76082492206897E-5	3.5276476918495324E-4
74-75	13.282960523294081	9.312989906482765E-4	0.0	8.995501614216307E-5	3.580562407227275E-4
76-77	14.231634942648562	9.595201721830729E-4	0.0	9.171883998808784E-5	3.633477122605018E-4
78-79	15.18859051495966	9.842137060260196E-4	0.0	9.171883998808784E-5	3.6687535995235136E-4
80-81	16.161002475632596	0.0010106710637148909	0.0	9.171883998808784E-5	3.7216683149012567E-4
82-83	17.137672307069597	0.0010283093021741386	0.0	9.171883998808784E-5	3.8274977456567423E-4
84-85	18.124690493010636	0.0010618219552467091	0.0	9.524648767993737E-5	3.9333271764122285E-4
86-87	19.120928186194327	0.0011059175513948283	0.0	9.524648767993737E-5	4.056794845626962E-4
88	19.866149052569078	0.0011217919660081513	0.0	9.524648767993737E-5	4.092071322545457E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	92120	0.0	78.1753	8
GTATCAA	92880	0.0	77.31302	9
GTGGTAT	93375	0.0	77.23044	6
TGGTATC	94000	0.0	76.49383	7
AGTGGTA	94945	0.0	76.11613	5
AAGCAGT	102940	0.0	71.10389	1
CAGTGGT	105960	0.0	68.62168	4
GCAGTGG	107160	0.0	68.40604	3
AGCAGTG	113400	0.0	64.79412	2
CAACGCA	89295	0.0	42.89467	12-13
ATCAACG	91430	0.0	41.978027	10-11
ACGCAGA	93010	0.0	41.315002	14-15
TCAACGC	88590	0.0	40.52283	12-13
AGAGTAC	93535	0.0	40.444427	18-19
AACGCAG	91395	0.0	39.27376	14-15
GAGTACT	87665	0.0	39.150215	20-21
AGTACTT	95280	0.0	38.390926	20-21
TATCAAC	94870	0.0	37.848156	10-11
CGCAGAG	95240	0.0	37.668644	16-17
GTACTTT	93610	0.0	36.605583	22-23
>>END_MODULE