FastQCFastQC Report
Thu 26 May 2016
SRR1660679_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660679_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28347502
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8927143.1491804815817632No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTGCATCTCGTATG415520.14658081689173177TruSeq Adapter, Index 14 (97% over 37bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT341320.12040567101820825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1213500.080.3031858
GTGGTAT1229750.079.410716
GTATCAA1225500.079.286829
AGTGGTA1239750.079.0600365
TGGTATC1234400.078.909297
AAGCAGT1297650.076.319581
GCAGTGG1357350.072.815173
CAGTGGT1352000.072.783364
AGCAGTG1422750.069.7646262
CAACGCA1182600.043.66003412-13
ATCAACG1199300.042.99918710-11
ACGCAGA1222150.042.35185214-15
AGAGTAC1241200.041.4848218-19
TCAACGC1176500.041.1647212-13
AACGCAG1204350.040.2468214-15
GAGTACT1176850.039.9747620-21
AGTACTT1257400.039.9047220-21
TATCAAC1238900.039.20514710-11
CGCAGAG1244350.038.9043616-17
GTACTTT1232800.038.0786422-23
Produced by FastQC (version 0.11.5)