##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660679_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28347502 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67733107488625 34.0 31.0 34.0 31.0 34.0 2 32.89301004370685 34.0 31.0 34.0 31.0 34.0 3 32.94977733840534 34.0 31.0 34.0 31.0 34.0 4 36.33193072885223 37.0 37.0 37.0 35.0 37.0 5 36.267833652503136 37.0 37.0 37.0 35.0 37.0 6 36.24752092794631 37.0 37.0 37.0 35.0 37.0 7 36.20001053355601 37.0 37.0 37.0 35.0 37.0 8 36.221062864727905 37.0 37.0 37.0 35.0 37.0 9 37.986878244157104 39.0 38.0 39.0 35.0 39.0 10-11 37.922285515669074 39.0 38.0 39.0 35.0 39.0 12-13 37.90937520702883 39.0 38.0 39.0 35.0 39.0 14-15 39.45211403459818 41.0 39.0 41.0 36.0 41.0 16-17 39.4255798094661 41.0 39.0 41.0 36.0 41.0 18-19 39.40079951312818 41.0 39.0 41.0 36.0 41.0 20-21 39.36193672373672 41.0 39.0 41.0 36.0 41.0 22-23 39.24792283284785 40.5 39.0 41.0 36.0 41.0 24-25 39.23975929166528 40.0 39.0 41.0 36.0 41.0 26-27 39.17989641556424 40.0 39.0 41.0 36.0 41.0 28-29 39.11169313966359 40.0 39.0 41.0 36.0 41.0 30-31 38.98555946834398 40.0 39.0 41.0 35.0 41.0 32-33 38.913347585265186 40.0 38.0 41.0 35.0 41.0 34-35 38.839537095720104 40.0 38.0 41.0 35.0 41.0 36-37 38.70235906500686 40.0 38.0 41.0 35.0 41.0 38-39 38.42958804624125 40.0 38.0 41.0 34.5 41.0 40-41 38.3107713864876 40.0 38.0 41.0 34.0 41.0 42-43 38.16291897607063 40.0 37.5 41.0 34.0 41.0 44-45 38.04120477705584 40.0 37.0 41.0 33.5 41.0 46-47 37.892896647471794 40.0 37.0 41.0 33.0 41.0 48-49 37.685341057564784 40.0 36.5 41.0 33.0 41.0 50-51 37.71263194548853 40.0 36.5 41.0 33.0 41.0 52-53 37.61722844220983 40.0 36.0 41.0 33.0 41.0 54-55 37.225793563750344 39.5 36.0 41.0 32.0 41.0 56-57 36.84131601789815 39.0 35.5 41.0 31.0 41.0 58-59 36.58752737719182 39.0 35.0 41.0 31.0 41.0 60-61 36.340486685564045 39.0 35.0 41.0 30.5 41.0 62-63 36.07130582440739 38.0 35.0 40.0 30.0 41.0 64-65 35.86320794685895 37.5 35.0 40.0 30.0 41.0 66-67 35.52079107358384 37.0 35.0 40.0 30.0 41.0 68-69 35.29118632745841 36.5 35.0 39.0 30.0 41.0 70-71 34.97511770172906 36.0 34.0 39.0 29.0 41.0 72-73 34.61881921729823 35.5 34.0 38.5 29.0 40.0 74-75 34.24113734959786 35.0 34.0 37.0 29.0 39.5 76-77 32.80912757321615 34.5 32.0 36.0 26.5 39.0 78-79 33.517217584110234 35.0 34.0 36.0 28.0 39.0 80-81 33.40926944815102 35.0 34.0 36.0 28.5 38.0 82-83 33.20618538099053 35.0 34.0 36.0 28.0 37.0 84-85 32.990578870053525 35.0 34.0 35.0 27.5 37.0 86-87 32.80260243036582 35.0 34.0 35.0 27.0 36.0 88-89 32.6361501447288 35.0 33.5 35.0 27.0 36.0 90-91 32.50555114168437 35.0 33.0 35.0 26.5 36.0 92-93 32.39476584215427 35.0 33.0 35.0 26.0 35.5 94-95 32.28907099115823 35.0 33.0 35.0 25.5 35.0 96-97 32.20717493908282 35.0 33.0 35.0 25.0 35.0 98-99 32.12708574815517 35.0 33.0 35.0 25.0 35.0 100 32.00791988655649 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06759873207629852 1101 2 0.12241916383843687 1101 3 0.16188980712867362 1101 4 0.1159441700549948 1101 5 0.1392064681744145 1101 6 0.13361242461764533 1101 7 0.16840053722891923 1101 8 0.13544182725463827 1101 9 0.18440764594792824 1101 10-11 0.16276858673240469 1101 12-13 0.20866331460079124 1101 14-15 0.23359386124280235 1101 16-17 0.22305721599730788 1101 18-19 0.23306831806811346 1101 20-21 0.21936807756472376 1101 22-23 0.24711519707105367 1101 24-25 0.26172295528596123 1101 26-27 0.2547727430400286 1101 28-29 0.28062504651200726 1101 30-31 0.3350762635342903 1101 32-33 0.3084678008016013 1101 34-35 0.329516419194789 1101 36-37 0.3334332279530443 1101 38-39 0.32038923786090834 1101 40-41 0.3765032751515278 1101 42-43 0.39219097759207955 1101 44-45 0.33180541948712516 1101 46-47 0.40117820789603087 1101 48-49 0.4797148073795796 1101 50-51 0.24191458209323713 1101 52-53 0.3347826721530467 1101 54-55 0.3627311354899021 1101 56-57 0.3886761805015695 1101 58-59 0.36532774076570007 1101 60-61 0.31926495460611193 1101 62-63 0.34079315229346463 1101 64-65 0.3019262561655296 1101 66-67 0.4110986852166292 1101 68-69 0.3543992687570636 1101 70-71 0.358628523345196 1101 72-73 0.3464149334703208 1101 74-75 0.3523043005519355 1101 76-77 0.6261322569551737 1101 78-79 0.4253124070443448 1101 80-81 0.3657256658155106 1101 82-83 0.3248986444599282 1101 84-85 0.34017084315328106 1101 86-87 0.3191586476541133 1101 88-89 0.3740668988065252 1101 90-91 0.38074584154018964 1101 92-93 0.41658500958010336 1101 94-95 0.4000151838740891 1101 96-97 0.3759524179148386 1101 98-99 0.4017856187411155 1101 100 0.4051167637951494 1102 1 -0.008929727916147101 1102 2 0.07962610822729488 1102 3 0.12276562432145255 1102 4 0.12008294299474898 1102 5 0.11777954723766015 1102 6 0.14260994836703844 1102 7 0.1117999996587642 1102 8 0.1312498005676659 1102 9 0.15930902696140237 1102 10-11 0.16534032313625602 1102 12-13 0.23148736529585534 1102 14-15 0.2129406884847853 1102 16-17 0.22376827304658065 1102 18-19 0.23308308981516745 1102 20-21 0.1981677510718285 1102 22-23 0.22059312505231077 1102 24-25 0.202175511654616 1102 26-27 0.24653687727412432 1102 28-29 0.28585772826269107 1102 30-31 0.29450249896057556 1102 32-33 0.27318934131393036 1102 34-35 0.2867023466726337 1102 36-37 0.30535115230042464 1102 38-39 0.3290608016341281 1102 40-41 0.3057332564446824 1102 42-43 0.3243023581507174 1102 44-45 0.39089532509122193 1102 46-47 0.38704787564720533 1102 48-49 0.4199624295225348 1102 50-51 0.3364177019796557 1102 52-53 0.33431156056595057 1102 54-55 0.23703392501916198 1102 56-57 0.2157510035432466 1102 58-59 0.22129887030202866 1102 60-61 0.22134835264475328 1102 62-63 0.23946894202726554 1102 64-65 0.19967620163475175 1102 66-67 0.2731032598983276 1102 68-69 0.26792023394263254 1102 70-71 0.2399154724063024 1102 72-73 0.22560023490788694 1102 74-75 0.2390543193301653 1102 76-77 0.5002664432696804 1102 78-79 0.3528243647389573 1102 80-81 0.2844241592956749 1102 82-83 0.27940293022817997 1102 84-85 0.28975725943727326 1102 86-87 0.2756979490153597 1102 88-89 0.3122389416350302 1102 90-91 0.3594218917507561 1102 92-93 0.31975736082759454 1102 94-95 0.33417955209690575 1102 96-97 0.33974663227175483 1102 98-99 0.3436917036724978 1102 100 0.377299325398603 1103 1 -0.09408580698563185 1103 2 -0.0017148733505294445 1103 3 -0.005166438050061117 1103 4 0.04905332907565452 1103 5 0.05245876814970529 1103 6 0.10041668250080704 1103 7 0.10020016005262988 1103 8 0.10259697853827987 1103 9 0.1224203547185354 1103 10-11 0.12446246911738257 1103 12-13 0.16705713302060587 1103 14-15 0.16431264732066353 1103 16-17 0.1386468464058268 1103 18-19 0.17147190411086655 1103 20-21 0.17781476700039178 1103 22-23 0.16654715319589997 1103 24-25 0.19022333430074667 1103 26-27 0.17859564696902197 1103 28-29 0.20246811484069127 1103 30-31 0.1985750439906795 1103 32-33 0.20464148327189946 1103 34-35 0.25723100459319426 1103 36-37 0.24327635304830864 1103 38-39 0.28362390749106936 1103 40-41 0.27605723544892413 1103 42-43 0.3299890989085057 1103 44-45 0.33137161998833164 1103 46-47 0.2962692468371202 1103 48-49 0.376182732196888 1103 50-51 0.3435105267460443 1103 52-53 0.31548930635714356 1103 54-55 0.33148060230763576 1103 56-57 0.34128947470471616 1103 58-59 0.3392164162728548 1103 60-61 0.3294130195261502 1103 62-63 0.35887748152570254 1103 64-65 0.3303139999615752 1103 66-67 0.3812067261110599 1103 68-69 0.30138232968091927 1103 70-71 0.289875350827721 1103 72-73 0.2707494591759101 1103 74-75 0.2649940687907488 1103 76-77 0.643661533443364 1103 78-79 0.44716101440536704 1103 80-81 0.4036784015770465 1103 82-83 0.36848369653969826 1103 84-85 0.34598818255405916 1103 86-87 0.3692475142001186 1103 88-89 0.4254925740759816 1103 90-91 0.42002536619882136 1103 92-93 0.4283578434465909 1103 94-95 0.41532170998719664 1103 96-97 0.448664658893442 1103 98-99 0.46965327654742595 1103 100 0.4537428505008947 1104 1 -0.0334488489521263 1104 2 0.04563815600056387 1104 3 0.06480496016099835 1104 4 0.06909287587642154 1104 5 0.0650046331140075 1104 6 0.07167844113586597 1104 7 0.07912741988689476 1104 8 0.08091319842726818 1104 9 0.06884250763422273 1104 10-11 0.07615870830948523 1104 12-13 0.13159591215224964 1104 14-15 0.12296165575825313 1104 16-17 0.07531904600094208 1104 18-19 0.09602971179069897 1104 20-21 0.13173335226731808 1104 22-23 0.11407228538313774 1104 24-25 0.11456702162015375 1104 26-27 0.12993988621027341 1104 28-29 0.13638788743165975 1104 30-31 0.18258739454756068 1104 32-33 0.1953335467490902 1104 34-35 0.1857979921059325 1104 36-37 0.17053000534117757 1104 38-39 0.15191548962282297 1104 40-41 0.18891681069154487 1104 42-43 0.24390235764331436 1104 44-45 0.23055791495968947 1104 46-47 0.22131351958110912 1104 48-49 0.32480177146170774 1104 50-51 0.241318214490569 1104 52-53 0.22497856349867362 1104 54-55 0.18340828899331996 1104 56-57 0.17063915844010324 1104 58-59 0.1647410159870688 1104 60-61 0.1937190711596415 1104 62-63 0.1755033466267477 1104 64-65 0.15842469724807273 1104 66-67 0.22083598186886633 1104 68-69 0.15420835159433466 1104 70-71 0.16282196604157662 1104 72-73 0.12891953346269958 1104 74-75 0.17849846771650135 1104 76-77 0.38963673547859656 1104 78-79 0.2988990709537376 1104 80-81 0.2465630302943893 1104 82-83 0.20659472559846392 1104 84-85 0.19474311748711415 1104 86-87 0.21629262456562515 1104 88-89 0.2323586818022676 1104 90-91 0.2696850337709904 1104 92-93 0.28098010268217877 1104 94-95 0.2703382495643467 1104 96-97 0.2691814049250283 1104 98-99 0.3207724013341178 1104 100 0.3295440267160643 1105 1 -0.07105293943626378 1105 2 -0.012291652258440422 1105 3 -0.009266691235637836 1105 4 0.03590504102488268 1105 5 0.035960656713797334 1105 6 0.02544809004628945 1105 7 0.02721144261665387 1105 8 0.005553841412954341 1105 9 0.06335877056334027 1105 10-11 0.057160673982856736 1105 12-13 0.1040467011444619 1105 14-15 0.09180574246988726 1105 16-17 0.0999759423963198 1105 18-19 0.09801114616045226 1105 20-21 0.07570342396900998 1105 22-23 0.10671754078948936 1105 24-25 0.0814826548612686 1105 26-27 0.09901174701488458 1105 28-29 0.12160700921771905 1105 30-31 0.133961082241548 1105 32-33 0.15516578663380898 1105 34-35 0.1512513950230172 1105 36-37 0.17350842708049896 1105 38-39 0.1877139702633741 1105 40-41 0.1885644286260444 1105 42-43 0.18894404707173607 1105 44-45 0.2256572198299267 1105 46-47 0.26511459753805866 1105 48-49 0.3236539489824537 1105 50-51 0.27100731794920563 1105 52-53 0.2679617108105603 1105 54-55 0.24482650010041596 1105 56-57 0.15889098207573937 1105 58-59 0.1658772685479235 1105 60-61 0.20987046599427828 1105 62-63 0.19282735610109825 1105 64-65 0.20253463187552256 1105 66-67 0.23752710085550888 1105 68-69 0.20414343634007537 1105 70-71 0.20458381056837993 1105 72-73 0.16969298586872839 1105 74-75 0.15535269191708778 1105 76-77 0.5510155835364117 1105 78-79 0.3792604414650498 1105 80-81 0.30632352913570315 1105 82-83 0.2865755380905881 1105 84-85 0.25088049004821045 1105 86-87 0.2881361540557563 1105 88-89 0.2598362139875263 1105 90-91 0.2819109039634071 1105 92-93 0.30360279567612736 1105 94-95 0.31904591841455954 1105 96-97 0.2839788173340878 1105 98-99 0.31291635367932 1105 100 0.35873346847853327 1106 1 -0.08083508836548958 1106 2 -0.03536282769142929 1106 3 -0.015761610963167527 1106 4 0.03244167640435336 1106 5 0.016067112973765063 1106 6 0.0435559128932681 1106 7 0.03658293403555746 1106 8 0.01055705721350364 1106 9 0.020704622695348007 1106 10-11 0.03236138355082829 1106 12-13 0.06684203558863544 1106 14-15 0.030737351627827536 1106 16-17 0.04193384270202927 1106 18-19 0.006196411349883135 1106 20-21 0.03237217387215452 1106 22-23 0.06294651311554134 1106 24-25 0.0847943423546269 1106 26-27 0.09318576912009036 1106 28-29 0.0501064975581329 1106 30-31 0.11011026384449707 1106 32-33 0.10432449924972076 1106 34-35 0.11424239809011283 1106 36-37 0.09929472795699468 1106 38-39 0.1044015668349374 1106 40-41 0.13865832172262316 1106 42-43 0.14344319859203125 1106 44-45 0.17641929687732727 1106 46-47 0.20492388889731927 1106 48-49 0.21604386761990924 1106 50-51 0.1751217567068295 1106 52-53 0.14394847763377072 1106 54-55 0.09672264873780989 1106 56-57 0.13395191852873012 1106 58-59 0.15713445963370276 1106 60-61 0.13822402199816963 1106 62-63 0.13907985720297233 1106 64-65 0.13766183871315008 1106 66-67 0.18170531581071003 1106 68-69 0.15316905710810147 1106 70-71 0.130110256406482 1106 72-73 0.14015750135239813 1106 74-75 0.09978055912584693 1106 76-77 0.44506548565012594 1106 78-79 0.24921086893608901 1106 80-81 0.20723105814613518 1106 82-83 0.2061960643755114 1106 84-85 0.18940257966353613 1106 86-87 0.21293151632404062 1106 88-89 0.22497636636401808 1106 90-91 0.2061696377578741 1106 92-93 0.22305155294945678 1106 94-95 0.22120510885784483 1106 96-97 0.22273318058157798 1106 98-99 0.2760262686467101 1106 100 0.28068552572080563 1107 1 -0.0838848179256928 1107 2 -0.025233160058093063 1107 3 0.01351116242157957 1107 4 0.05080201676421581 1107 5 0.017802478532239263 1107 6 0.04712493329327572 1107 7 0.038066649607593206 1107 8 0.022004693558493216 1107 9 0.028862240182114363 1107 10-11 0.02353687378857927 1107 12-13 0.0761531795804089 1107 14-15 0.021978251727141185 1107 16-17 0.07471120377328333 1107 18-19 0.06914437682410579 1107 20-21 0.0500581768399897 1107 22-23 0.02811560882339137 1107 24-25 0.06045999365206711 1107 26-27 0.0627333911888357 1107 28-29 0.06728138336860923 1107 30-31 0.11951396308565876 1107 32-33 0.09212526424111189 1107 34-35 0.06203806492425201 1107 36-37 0.0785185409624205 1107 38-39 0.08119750945246551 1107 40-41 0.10041004074511051 1107 42-43 0.12194446805554549 1107 44-45 0.14976712130206238 1107 46-47 0.16530396317671858 1107 48-49 0.24204588592245102 1107 50-51 0.1704431975713092 1107 52-53 0.1442418174810811 1107 54-55 0.08092365235863497 1107 56-57 0.06299870677448638 1107 58-59 0.08635041744252447 1107 60-61 0.11596028278319181 1107 62-63 0.15452160610420407 1107 64-65 0.1455464372739712 1107 66-67 0.15884101978798526 1107 68-69 0.11726894613499894 1107 70-71 0.07053225016580456 1107 72-73 0.1114986609659141 1107 74-75 0.1485057432052912 1107 76-77 0.27641606441255817 1107 78-79 0.22993675769605204 1107 80-81 0.1925320427059134 1107 82-83 0.2066761119828513 1107 84-85 0.1913513715387225 1107 86-87 0.15366258201443372 1107 88-89 0.16054908194712425 1107 90-91 0.19804961362665097 1107 92-93 0.2257005551644582 1107 94-95 0.23365778385928593 1107 96-97 0.23387403216624847 1107 98-99 0.268031017195419 1107 100 0.3042861253555529 1108 1 -0.15215757800450547 1108 2 -0.08599310701991669 1108 3 -0.06860754635466293 1108 4 -0.020540927599824954 1108 5 -0.03981138850704724 1108 6 -0.004769577247841994 1108 7 -0.010880346474088753 1108 8 -0.05480280374829505 1108 9 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-0.18582949998096154 2316 72-73 -0.18732981460075848 2316 74-75 -0.14767970052682244 2316 76-77 -0.5275443015585282 2316 78-79 -0.25171977770592946 2316 80-81 -0.16685657649596664 2316 82-83 -0.2687372987803869 2316 84-85 -0.26360907357463503 2316 86-87 -0.2778156103080036 2316 88-89 -0.3139128301428826 2316 90-91 -0.23693227601860656 2316 92-93 -0.20246747123526632 2316 94-95 -0.13674486574531386 2316 96-97 -0.16486332891687994 2316 98-99 -0.03821031799662222 2316 100 -0.1283517423733791 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 18.0 7 254.0 8 431.0 9 140.0 10 47.0 11 37.0 12 122.0 13 369.0 14 1403.0 15 4123.0 16 9227.0 17 15183.0 18 20672.0 19 27011.0 20 36318.0 21 52770.0 22 80329.0 23 133807.0 24 224927.0 25 317751.0 26 349747.0 27 325323.0 28 312777.0 29 349350.0 30 418613.0 31 494477.0 32 616078.0 33 813014.0 34 1159362.0 35 1857805.0 36 3414330.0 37 7047722.0 38 8349718.0 39 1911848.0 40 2399.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.418839372412464 27.59759419235104 29.300351099035332 26.683215336201165 2 23.22168332341071 32.01640465570181 24.575539555144534 20.186372465742945 3 24.839571402093913 24.38614167837434 26.931960354037543 23.8423265654942 4 20.20723124413437 27.590616458345423 23.354301707670842 28.847850589849365 5 23.846360625919637 29.248379974155785 25.915495839561263 20.989763560363315 6 29.109303974246124 27.835322342537044 23.912474752278115 19.142898930938713 7 23.381682802244796 24.96983332076315 29.11106594154222 22.53741793544983 8 26.893946422510172 27.144998525796026 24.058663087844565 21.90239196384923 9 25.16455594570555 24.685116875553973 27.35944070133587 22.790886477404605 10-11 22.60176928464455 28.152198384182142 26.971545852611634 22.274486478561673 12-13 22.36084682170584 26.343806237318546 27.08457344848234 24.21077349249327 14-15 22.201072602446594 29.912753864520408 25.485233231485445 22.40094030154756 16-17 24.13181944567814 25.996048964032177 25.629371152350565 24.24276043793912 18-19 22.233974972468474 27.723680908462413 25.917911567657708 24.12443255141141 20-21 23.93919421830242 27.988870257553973 25.824725253268348 22.24721027087526 22-23 24.083208245254877 26.250784067750367 27.39623116213401 22.269776524860745 24-25 22.359456466529398 27.68679551277222 25.566072811066103 24.387675209632278 26-27 22.393816216610578 25.791507131831352 29.438005977620584 22.376670673937483 28-29 22.384297662140916 25.990162463761806 29.25524614240577 22.3702937316915 30-31 22.407991058438505 25.727450864733836 29.50572415725882 22.358833919568845 32-33 22.454724855182736 26.1147662904328 29.061326466699388 22.369182387685075 34-35 22.486344064452616 26.136993909818578 28.995453442536135 22.381208583192674 36-37 22.46583751736263 26.08798902395008 29.1993062462497 22.246867212437586 38-39 22.543496756361858 26.092567905008135 29.24117981754456 22.122755521085445 40-41 22.457575305354432 26.25643457224978 29.019337849837694 22.26665227255809 42-43 22.433871829647913 26.156817487821694 29.134798953944856 22.274511728585537 44-45 22.512675184212654 26.293457412208326 29.03297928315091 22.16088812042811 46-47 22.70164113836703 26.36563797581498 28.867314024325434 22.06540686149255 48-49 22.588744054811944 26.559585661863856 28.794230555109067 22.057439728215137 50-51 22.7780293451225 26.551168471562647 28.5251666299709 22.14563555334395 52-53 22.8677361796224 26.64283707999458 28.258160367105074 22.23126637327794 54-55 22.873245267021517 26.868481567259412 27.85791057080409 22.400362594914984 56-57 23.197621311715466 27.278548341140844 27.216214219732553 22.307616127411134 58-59 23.265061618338493 27.5179300211127 26.699784072775635 22.51722428777317 60-61 23.31333769665531 27.6592342740624 26.477183308206985 22.55024472107531 62-63 23.16573654663697 27.7923330852323 26.614660864905037 22.427269503225688 64-65 23.32127989301693 27.930848703982697 26.245429186048437 22.502442216951938 66-67 23.114611242999906 27.93015128182533 26.406841290638322 22.548396184536436 68-69 23.017330122049216 27.908487718644988 26.494011448209996 22.5801707110958 70-71 22.989524058780862 27.75549380247795 26.58676402964677 22.66821810909442 72-73 22.867637676766858 27.6846581219175 26.666469442423246 22.781234758892392 74-75 22.92147803051119 27.698171097135866 26.57860110921206 22.801749763140887 76-77 22.960938678647302 27.64466923972295 26.461727047481325 22.93266503414842 78-79 22.9937183931935 27.63625824977393 26.333104640264395 23.036918716768177 80-81 22.996967595468252 27.70712248623764 26.188852980493348 23.107056937800756 82-83 22.981012081072457 27.663329421723144 26.06192632191464 23.293732175289758 84-85 22.94393048148669 27.56527392473859 26.058145084217543 23.432650509557174 86-87 22.940880445858582 27.51511794227115 26.035617034341797 23.508384577528474 88-89 22.93257699627652 27.361473728399506 26.03985409467971 23.666095180644263 90-91 22.907221877292997 27.209772040977594 26.12635992267314 23.756646159056274 92-93 22.921985093057838 27.134685896110422 26.18264255766839 23.760686453163345 94-95 22.924990318347685 26.980389124461844 26.232189128652706 23.862431428537764 96-97 22.84383632129386 26.886845342773302 26.329464448443364 23.939853887489477 98-99 22.828925277667143 26.881674172361485 26.331330632951776 23.958069917019596 100 22.76835916037637 26.82972671928205 26.313626818850878 24.08828730149071 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 35330.0 1 24579.5 2 10620.5 3 6625.0 4 5616.0 5 5244.0 6 5168.0 7 5263.0 8 5136.5 9 5279.0 10 5671.5 11 9169.0 12 88918.5 13 103804.0 14 39360.0 15 38602.5 16 43485.5 17 48647.0 18 54308.0 19 60605.5 20 67785.0 21 78362.0 22 93394.0 23 110779.5 24 129765.5 25 150822.5 26 174323.5 27 202707.0 28 239051.0 29 278708.0 30 314006.5 31 350911.0 32 390478.0 33 424741.5 34 456402.5 35 495446.0 36 543530.0 37 585244.5 38 628940.0 39 677452.5 40 716001.0 41 758683.0 42 812060.0 43 851054.5 44 881247.0 45 922115.0 46 963062.5 47 999627.0 48 1035241.5 49 1070053.5 50 1104791.5 51 1134136.5 52 1142514.5 53 1127969.0 54 1091929.0 55 1037391.5 56 972361.5 57 891698.5 58 792406.0 59 679237.5 60 559028.5 61 448449.0 62 352840.5 63 267017.5 64 197553.5 65 145174.5 66 104965.5 67 76856.0 68 56818.5 69 42801.0 70 32747.0 71 25388.0 72 20515.0 73 15821.5 74 12224.0 75 10252.5 76 7791.0 77 4553.0 78 2513.0 79 1548.5 80 925.0 81 573.5 82 352.5 83 197.5 84 126.0 85 89.0 86 65.5 87 38.0 88 21.0 89 14.0 90 10.5 91 8.5 92 7.5 93 6.5 94 4.0 95 2.0 96 1.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29419523455717544 2 0.02903254050392165 3 0.0 4 0.06073903795826525 5 0.0025822381104338575 6 0.0013757825998213175 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0013934208382805652 22-23 5.044536199344831E-4 24-25 2.345885715079939E-4 26-27 0.006852455641417717 28-29 0.006661962666057841 30-31 0.005891171645388719 32-33 0.0047535052647672445 34-35 0.004303730184056429 36-37 0.0050127873701181856 38-39 0.007654995491313485 40-41 0.009073109863436997 42-43 0.019054589007525248 44-45 0.005861186640007998 46-47 0.01165711179771678 48-49 0.009630478198749223 50-51 0.014415732292743115 52-53 0.01568039399027117 54-55 0.05701207817182622 56-57 0.049044885859784046 58-59 0.05387070790223421 60-61 0.07756944509607937 62-63 0.05276655417468531 64-65 0.0389999090572425 66-67 0.038518385147305045 68-69 0.032094538700447046 70-71 0.021640354765650953 72-73 0.014570948791184493 74-75 0.007286356307515209 76-77 0.0046406205386280595 78-79 0.005818854867705804 80-81 0.0018537788620669293 82-83 9.41881933723825E-4 84-85 0.0010247816544822891 86-87 0.001084751665243731 88-89 0.0012417319875310353 90-91 0.0010653496029385586 92-93 3.7216683149012567E-4 94-95 2.398800430457682E-4 96-97 3.54528593030878E-4 98-99 9.34826638340126E-5 100 9.771584106423204E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.8347502E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.56329248562003 #Duplication Level Percentage of deduplicated Percentage of total 1 76.39000370109342 36.33360089012703 2 11.341861230192885 10.789125260459567 3 4.2848249453738 6.114011463794848 4 2.2586048998849666 4.297067418507329 5 1.3552668017714564 3.2230475644353302 6 0.8810970742805384 2.5144726711337597 7 0.6268283172978686 2.0869813015735295 8 0.42703971541848007 1.6249131909940084 9 0.3395443007244014 1.4534860398462026 >10 1.8550237375466274 16.236138803761634 >50 0.1586985857656668 5.254285529344703 >100 0.07992678152210583 5.814735137135452 >500 8.107647187731953E-4 0.25643172865094044 >1k 4.174027262349136E-4 0.3159229334332903 >5k 7.391668955274687E-6 0.019027082727233992 >10k+ 4.435001367734423E-5 3.6667529840751834 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 892714 3.1491804815817632 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTGCATCTCGTATG 41552 0.14658081689173177 TruSeq Adapter, Index 14 (97% over 37bp) AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 34132 0.12040567101820825 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.2562836048128685 1.763823845924766E-5 0.0 0.0 1.0582943075548597E-5 2 0.25631182599440333 2.4693533842946725E-5 0.0 0.0 1.0582943075548597E-5 3 0.25632946423286257 2.822118153479626E-5 0.0 0.0 1.763823845924766E-5 4 0.25634357482362996 3.880412461034485E-5 0.0 0.0 2.822118153479626E-5 5 0.25637885130054844 7.408060152884018E-5 0.0 0.0 3.527647691849532E-5 6 0.25643529366361806 8.466354460438878E-5 0.0 3.5276476918495323E-6 4.585941999404392E-5 7 0.25643529366361806 9.87741353717869E-5 0.0 3.5276476918495323E-6 4.585941999404392E-5 8 0.2564388213113099 1.1288472613918503E-4 0.0 3.5276476918495323E-6 5.291471537774299E-5 9 0.256459987197461 1.2346766921473364E-4 0.0 3.5276476918495323E-6 6.349765845329158E-5 10-11 0.25652524867976023 1.4816120305768035E-4 0.0 8.81911922962383E-6 6.526148229921635E-5 12-13 0.25659932928128903 1.6050796997915372E-4 0.0 1.2346766921473363E-5 7.231677768291541E-5 14-15 0.2567033948881986 1.7991003228432615E-4 0.0 1.763823845924766E-5 8.642736845031354E-5 16-17 0.2568180434381837 1.975482707435738E-4 0.0 1.763823845924766E-5 9.171883998808784E-5 18-19 0.25694503875509034 2.2753327612429485E-4 0.0 1.763823845924766E-5 1.0406560690956121E-4 20-21 0.2571655167358309 2.539906338131663E-4 0.0 1.763823845924766E-5 1.1288472613918503E-4 22-23 0.2578340059734364 2.910309345775864E-4 0.0 1.940206230517243E-5 1.252314930606584E-4 24-25 0.2605802797015413 3.2101593995830744E-4 0.0 2.292970999702196E-5 1.411059076739813E-4 26-27 0.26989326960802407 3.2630741149608175E-4 0.0 2.4693533842946725E-5 1.569803222873042E-4 28-29 0.2947120349440314 3.3689035457163037E-4 0.0 2.4693533842946725E-5 1.6932708920877756E-4 30-31 0.34777667534867795 3.5276476918495324E-4 0.0 2.4693533842946725E-5 1.7461856074655187E-4 32-33 0.44086424264120344 3.6158388841457705E-4 0.0 2.822118153479626E-5 1.8872915151395E-4 34-35 0.5837110444511124 3.6687535995235136E-4 0.0 3.174882922664579E-5 2.0636738997319764E-4 36-37 0.7801004829279137 3.756944791819752E-4 0.0 3.704030076442009E-5 2.18714156894671E-4 38-39 1.0282599151064529 3.915688937952981E-4 0.0 3.880412461034485E-5 2.292970999702196E-4 40-41 1.3329446100753428 4.056794845626962E-4 0.0 4.0567948456269625E-5 2.3635239535391866E-4 42-43 1.6928264084786024 4.2508154686786865E-4 0.0 4.7623243839968685E-5 2.4340769073761772E-4 44-45 2.105067317748139 4.409559614811915E-4 0.0 5.1150891531818215E-5 2.698650484264892E-4 46-47 2.5756414092500988 4.762324383996868E-4 0.0 5.644236306959252E-5 2.822118153479626E-4 48-49 3.102587310867815 5.044536199344831E-4 0.0 5.644236306959252E-5 2.875032868857369E-4 50-51 3.679719292373628 5.450215683907528E-4 0.0 5.644236306959252E-5 2.9455858226943595E-4 52-53 4.303469138127232 5.608959830040757E-4 0.0 5.644236306959252E-5 2.980862299612855E-4 54-55 4.9659507917134995 5.80298045309248E-4 0.0 5.997001076144205E-5 3.0337770149905976E-4 56-57 5.667088408707053 6.155745222277434E-4 0.0 5.997001076144205E-5 3.104329968827588E-4 58-59 6.412914266660957 6.33212760686991E-4 0.0 6.349765845329158E-5 3.2277976380423224E-4 60-61 7.19566401300545 6.543786468380882E-4 0.0 6.526148229921635E-5 3.4041800226347987E-4 62-63 8.00716585186236 6.614339422217873E-4 0.0 6.702530614514111E-5 3.5276476918495324E-4 64-65 8.843585230190653 6.773083568351102E-4 0.0 7.055295383699064E-5 3.651115361064266E-4 66-67 9.70987496534968 6.843636522188092E-4 0.0 7.055295383699064E-5 3.739306553360505E-4 68-69 10.60106812938932 6.843636522188092E-4 0.0 7.055295383699064E-5 3.7922212687382473E-4 70-71 11.511624551609522 6.967104191402827E-4 0.0 7.055295383699064E-5 3.8274977456567423E-4 72-73 12.442029283567914 7.196401291373046E-4 0.0 7.584442537476494E-5 3.915688937952981E-4 74-75 13.386666310139073 7.372783675965522E-4 0.0 8.113589691253925E-5 3.950965414871476E-4 76-77 14.342946337917184 7.61971901439499E-4 0.0 8.113589691253925E-5 4.0744330840862097E-4 78-79 15.309248412787834 7.84901611436521E-4 0.0 8.113589691253925E-5 4.127347799463953E-4 80-81 16.292564332476278 8.113589691253924E-4 0.0 8.466354460438878E-5 4.1449860379232003E-4 82-83 17.280251007654925 8.325248552764897E-4 0.0 8.995501614216307E-5 4.1979007533009434E-4 84-85 18.278247233213 8.678013321949849E-4 0.0 9.171883998808784E-5 4.2508154686786865E-4 86-87 19.28708392012813 8.889672183460822E-4 0.0 9.524648767993737E-5 4.286091945597182E-4 88 20.041885877633945 8.960225137297812E-4 0.0 9.524648767993737E-5 4.3390066609749246E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 121350 0.0 80.303185 8 GTGGTAT 122975 0.0 79.41071 6 GTATCAA 122550 0.0 79.28682 9 AGTGGTA 123975 0.0 79.060036 5 TGGTATC 123440 0.0 78.90929 7 AAGCAGT 129765 0.0 76.31958 1 GCAGTGG 135735 0.0 72.81517 3 CAGTGGT 135200 0.0 72.78336 4 AGCAGTG 142275 0.0 69.764626 2 CAACGCA 118260 0.0 43.660034 12-13 ATCAACG 119930 0.0 42.999187 10-11 ACGCAGA 122215 0.0 42.351852 14-15 AGAGTAC 124120 0.0 41.48482 18-19 TCAACGC 117650 0.0 41.16472 12-13 AACGCAG 120435 0.0 40.24682 14-15 GAGTACT 117685 0.0 39.97476 20-21 AGTACTT 125740 0.0 39.90472 20-21 TATCAAC 123890 0.0 39.205147 10-11 CGCAGAG 124435 0.0 38.90436 16-17 GTACTTT 123280 0.0 38.07864 22-23 >>END_MODULE