FastQCFastQC Report
Thu 26 May 2016
SRR1660678_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660678_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30162225
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11634533.857318218400665No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA534940.1773542900101037No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC427430.14171036785250424Illumina Single End PCR Primer 1 (100% over 50bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT307520.10195534314859066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1544250.083.088298
GTGGTAT1560150.082.296366
GTATCAA1563900.081.771769
AGTGGTA1575900.081.650765
TGGTATC1573500.081.4170767
AAGCAGT1628300.079.954771
GCAGTGG1678650.077.391563
CAGTGGT1690950.076.394874
AGCAGTG1753600.074.416322
CAACGCA1517450.044.17248512-13
ATCAACG1534550.043.71766310-11
ACGCAGA1559900.042.95533814-15
AGAGTAC1556500.042.5727818-19
TCAACGC1507400.042.38780612-13
AACGCAG1534050.041.55494314-15
GAGTACT1488750.041.1234620-21
AGTACTT1577450.040.9303620-21
TATCAAC1581550.040.39975710-11
CGCAGAG1588700.040.0530216-17
GTACTTT1556350.039.29363322-23
Produced by FastQC (version 0.11.5)