FastQCFastQC Report
Thu 26 May 2016
SRR1660678_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660678_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30162225
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10250103.398323565320529No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA415470.1377451431384787No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT321240.1065040791917705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1379350.081.8888858
GTGGTAT1396050.081.0001756
GTATCAA1403150.080.322439
TGGTATC1409000.080.082317
AGTGGTA1421300.080.060335
AAGCAGT1463600.078.51561
GCAGTGG1514500.075.6578143
CAGTGGT1532250.074.5544744
AGCAGTG1584350.072.700822
CAACGCA1356000.043.84795812-13
ATCAACG1369450.043.30580510-11
ACGCAGA1400500.042.58679214-15
AGAGTAC1413300.042.02119418-19
TCAACGC1342300.041.81907312-13
AACGCAG1373550.040.97828314-15
GAGTACT1353350.040.3323520-21
AGTACTT1438850.040.2805620-21
TATCAAC1424600.039.53013210-11
CGCAGAG1426900.039.44048316-17
GTACTTT1411050.038.54662322-23
Produced by FastQC (version 0.11.5)