FastQCFastQC Report
Thu 26 May 2016
SRR1660677_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660677_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25539141
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7496712.9353806379000766No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC954350.3736813231110631Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA818270.3203984033762138No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1013150.081.390018
GTGGTAT1037200.079.8462756
TGGTATC1034100.079.8317647
GTATCAA1034050.079.5478449
AGTGGTA1046150.079.255345
AAGCAGT1109450.075.382161
GCAGTGG1142950.073.1841663
CAGTGGT1139300.073.059884
AGCAGTG1212550.069.329322
CAACGCA985000.043.82571812-13
ATCAACG1000600.043.3191810-11
ACGCAGA1021100.042.49928714-15
TCAACGC979350.041.81086312-13
AGAGTAC1034000.041.7095418-19
AACGCAG1005450.040.7754614-15
GAGTACT975300.040.4317420-21
AGTACTT1043650.039.90546420-21
CGCAGAG1044150.039.28235616-17
TATCAAC1050200.039.20545210-11
GTACTTT1031350.038.1254322-23
Produced by FastQC (version 0.11.5)