##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660677_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25539141
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45797953815283	34.0	31.0	34.0	31.0	34.0
2	32.58573375666786	34.0	31.0	34.0	31.0	34.0
3	32.57987631612199	34.0	31.0	34.0	31.0	34.0
4	35.87233027923688	37.0	37.0	37.0	35.0	37.0
5	35.8534785488674	37.0	37.0	37.0	35.0	37.0
6	35.83895754363861	37.0	37.0	37.0	35.0	37.0
7	35.804459985557074	37.0	37.0	37.0	35.0	37.0
8	35.784596005010506	37.0	37.0	37.0	35.0	37.0
9	37.48147586483038	39.0	38.0	39.0	35.0	39.0
10-11	37.49820640012912	39.0	38.0	39.0	35.0	39.0
12-13	37.54229552983007	39.0	38.0	39.0	35.0	39.0
14-15	39.05503904771112	41.0	40.0	41.0	36.0	41.0
16-17	39.04242437128171	41.0	40.0	41.0	36.0	41.0
18-19	39.005493234874265	41.0	39.5	41.0	36.0	41.0
20-21	38.9008110139648	41.0	39.0	41.0	35.5	41.0
22-23	38.843542917124736	41.0	39.0	41.0	35.0	41.0
24-25	38.86606262520732	41.0	39.0	41.0	35.0	41.0
26-27	38.85012273905375	41.0	39.0	41.0	35.0	41.0
28-29	38.800137228577896	41.0	39.0	41.0	35.0	41.0
30-31	38.71077269200244	41.0	39.0	41.0	35.0	41.0
32-33	38.63623980148745	40.5	39.0	41.0	35.0	41.0
34-35	38.561164194989956	40.0	38.5	41.0	34.0	41.0
36-37	38.445244184211205	40.0	38.0	41.0	34.0	41.0
38-39	38.17798055541492	40.0	38.0	41.0	34.0	41.0
40-41	38.07367309260715	40.0	38.0	41.0	33.0	41.0
42-43	37.9073389743218	40.0	37.5	41.0	33.0	41.0
44-45	37.76519948341254	40.0	37.0	41.0	33.0	41.0
46-47	37.69104258440016	40.0	37.0	41.0	33.0	41.0
48-49	37.730072205639175	40.0	37.0	41.0	33.0	41.0
50-51	37.029064661963375	39.0	36.0	40.5	32.0	41.0
52-53	37.022577756236984	39.5	36.0	40.0	32.0	41.0
54-55	37.177282215560815	40.0	36.0	41.0	31.0	41.0
56-57	36.8892100952025	40.0	36.0	41.0	30.5	41.0
58-59	36.66095414093998	39.0	35.0	41.0	30.0	41.0
60-61	36.42461903867479	39.0	35.0	41.0	30.0	41.0
62-63	36.136283890675884	39.0	35.0	41.0	29.5	41.0
64-65	35.82441825275173	37.5	35.0	40.5	29.0	41.0
66-67	35.479644342775664	37.0	35.0	40.0	29.0	41.0
68-69	35.127781627424355	36.5	35.0	39.5	28.5	41.0
70-71	34.7778250450945	36.0	34.5	39.0	28.0	41.0
72-73	34.42431648347139	35.5	34.0	39.0	28.0	41.0
74-75	34.161567434864	35.0	34.0	37.5	28.0	40.0
76-77	33.83145032168467	35.0	34.0	37.0	27.0	39.0
78-79	33.51072620257666	35.0	34.0	37.0	27.0	39.0
80-81	33.219682701935824	35.0	34.0	36.0	26.5	38.5
82-83	32.93920854659912	35.0	34.0	36.0	26.0	37.0
84-85	32.71208358182446	35.0	33.5	35.0	26.0	37.0
86-87	32.51907534791401	35.0	33.0	35.0	25.5	36.5
88-89	32.352525364889914	35.0	33.0	35.0	25.0	36.0
90-91	32.21980161744673	35.0	33.0	35.0	25.0	36.0
92-93	32.103466968603215	35.0	33.0	35.0	25.0	36.0
94-95	32.01625332660954	35.0	33.0	35.0	25.0	35.5
96-97	32.02581919258756	35.0	33.0	35.0	25.0	35.0
98-99	31.841141681311836	35.0	33.0	35.0	24.0	35.0
100	31.783816887185047	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.051630141780471206
1101	2	0.05002671392046665
1101	3	0.06712932205160627
1101	4	0.025038987540263236
1101	5	0.0672876409660148
1101	6	0.028691121572421707
1101	7	0.024944381704983698
1101	8	0.06631872803379224
1101	9	0.06420873486666068
1101	10-11	0.04967177900999786
1101	12-13	0.010255337527631525
1101	14-15	0.033338563102226715
1101	16-17	0.016760605617015756
1101	18-19	0.034599802443842975
1101	20-21	-0.004442839336967097
1101	22-23	0.05178649774398281
1101	24-25	0.0785569484541071
1101	26-27	0.10050781161582023
1101	28-29	0.054040856882146215
1101	30-31	0.11125506083000403
1101	32-33	0.1195091187344488
1101	34-35	0.09232249411035554
1101	36-37	0.09912387204795436
1101	38-39	0.15696838680803182
1101	40-41	0.1731717189775921
1101	42-43	0.15257660611045054
1101	44-45	0.13356750066689216
1101	46-47	0.12419022952900605
1101	48-49	0.08846014492547027
1101	50-51	0.17977830586603716
1101	52-53	0.1902819986045401
1101	54-55	0.14710555267402725
1101	56-57	0.12473748609642143
1101	58-59	0.17048084423893783
1101	60-61	0.1560998608608415
1101	62-63	0.13616320480522148
1101	64-65	0.12397472400310505
1101	66-67	0.10473915454916494
1101	68-69	0.09700777378017733
1101	70-71	0.09025398416271457
1101	72-73	0.07241015102950144
1101	74-75	0.11518117893248103
1101	76-77	0.13297307391301416
1101	78-79	0.14835791878315518
1101	80-81	0.12172391438398478
1101	82-83	0.16114501318746477
1101	84-85	0.14661610906214406
1101	86-87	0.15704113310236067
1101	88-89	0.12025040114961172
1101	90-91	0.143449223385403
1101	92-93	0.12614948629821043
1101	94-95	0.05670785816428037
1101	96-97	0.12041344949506083
1101	98-99	0.1522154822760875
1101	100	0.1839684848200278
1102	1	0.017949632597179743
1102	2	0.025914834901229256
1102	3	0.02573865479484283
1102	4	0.02822086188200501
1102	5	0.016305626461821987
1102	6	0.0011114869168906694
1102	7	-0.0063676849144584935
1102	8	0.0383049289685502
1102	9	0.07033124281888092
1102	10-11	0.013360682662828083
1102	12-13	-0.01326909971261614
1102	14-15	-3.3204444222434404E-4
1102	16-17	-0.002567104820272448
1102	18-19	0.044546498528156064
1102	20-21	0.0050950177114472694
1102	22-23	0.041153536371773214
1102	24-25	0.037371891488582776
1102	26-27	0.046221970652233324
1102	28-29	0.04351418536985818
1102	30-31	0.07046606058400329
1102	32-33	0.0709220500580443
1102	34-35	0.08838608396920478
1102	36-37	0.10122405749821439
1102	38-39	0.16074243878323813
1102	40-41	0.163015601391308
1102	42-43	0.12008645133571605
1102	44-45	0.09503285917310933
1102	46-47	0.08191477832781402
1102	48-49	0.03720385281680194
1102	50-51	0.037700759051674027
1102	52-53	0.13195366377063777
1102	54-55	0.14715302164596977
1102	56-57	0.15561538830615262
1102	58-59	0.11135835674371464
1102	60-61	0.13293906848485904
1102	62-63	0.13299494245620025
1102	64-65	0.09332553566319035
1102	66-67	0.1024584147391181
1102	68-69	0.09151029260839039
1102	70-71	0.06979998991390346
1102	72-73	0.06163163114921133
1102	74-75	0.09436516972486686
1102	76-77	0.12200960761513358
1102	78-79	0.07523698090840725
1102	80-81	0.12755048701328064
1102	82-83	0.15557459705928522
1102	84-85	0.1298260097680881
1102	86-87	0.15716901000732975
1102	88-89	0.1431402302094611
1102	90-91	0.14455082524559515
1102	92-93	0.14151329243034638
1102	94-95	0.11000643064478055
1102	96-97	0.1572891969197201
1102	98-99	0.1458987211019469
1102	100	0.1924161405122824
1103	1	0.019938468294725453
1103	2	0.02526634458010335
1103	3	0.002799787885592764
1103	4	0.01768744097228847
1103	5	0.03524948779083559
1103	6	0.00829597420056416
1103	7	-0.009324684195398447
1103	8	0.023819769641754363
1103	9	0.06560707242670105
1103	10-11	0.02341407585591071
1103	12-13	-0.003995787364125647
1103	14-15	0.024153700561463154
1103	16-17	0.01042315805943872
1103	18-19	0.06387613189667718
1103	20-21	0.029645765212499953
1103	22-23	0.06173333860432706
1103	24-25	0.03155205382057602
1103	26-27	0.050154944075138985
1103	28-29	0.07455111356535582
1103	30-31	0.04672391044626778
1103	32-33	0.03670333363248801
1103	34-35	0.07714051044838044
1103	36-37	0.09337033351099677
1103	38-39	0.12570057297660497
1103	40-41	0.10140123611216723
1103	42-43	0.11233712901579196
1103	44-45	0.11448244911717609
1103	46-47	0.07525079330251572
1103	48-49	0.07273474796178903
1103	50-51	0.12759873270857014
1103	52-53	0.16220092128607888
1103	54-55	0.13148281094688485
1103	56-57	0.11170615220710545
1103	58-59	0.11399441503632346
1103	60-61	0.1296740856444316
1103	62-63	0.09728191291876698
1103	64-65	0.11260046375956279
1103	66-67	0.08873688927094747
1103	68-69	0.10115361370434073
1103	70-71	0.0746645022513519
1103	72-73	0.08782478711724906
1103	74-75	0.1497084467920473
1103	76-77	0.12046897476164986
1103	78-79	0.09818885124425947
1103	80-81	0.08346631098918778
1103	82-83	0.14064878200530018
1103	84-85	0.12296127659122646
1103	86-87	0.09482861961165412
1103	88-89	0.09830932380561563
1103	90-91	0.13632881324519985
1103	92-93	0.10477053828073224
1103	94-95	0.0947201743768531
1103	96-97	0.17621098141604108
1103	98-99	0.16595157023913387
1103	100	0.16336741798444265
1104	1	-0.1374479296528932
1104	2	-0.14036078071428193
1104	3	-0.11963689007909295
1104	4	-0.10674609400492585
1104	5	-0.10448804946896217
1104	6	-0.11207098915820524
1104	7	-0.15750192000648866
1104	8	-0.09927613658741308
1104	9	-0.05400749097740487
1104	10-11	-0.10234790477547762
1104	12-13	-0.13347671292047636
1104	14-15	-0.14347763217167397
1104	16-17	-0.1619108393501989
1104	18-19	-0.1090413999283939
1104	20-21	-0.1554865596536814
1104	22-23	-0.0911541526701356
1104	24-25	-0.11119085686156893
1104	26-27	-0.08211258259113663
1104	28-29	-0.07346912788851512
1104	30-31	-0.05408852211602522
1104	32-33	-0.07160880756331522
1104	34-35	-0.10531390110114813
1104	36-37	-0.0755486013459361
1104	38-39	-0.027006143382315884
1104	40-41	-0.04365926093149852
1104	42-43	-0.06143438839390569
1104	44-45	-0.08292937190149985
1104	46-47	-0.044297956721550236
1104	48-49	-0.090951671201978
1104	50-51	0.0178989427256937
1104	52-53	-0.009322682082988365
1104	54-55	-0.04670919037039312
1104	56-57	-0.0347443247926833
1104	58-59	-0.03941721984180191
1104	60-61	-0.034852320894827926
1104	62-63	-0.05503893767208723
1104	64-65	-0.048263844136222644
1104	66-67	-0.040269784967435385
1104	68-69	-0.062339141944868004
1104	70-71	-0.07037373410352643
1104	72-73	-0.06555891785335888
1104	74-75	-0.0023087130565500047
1104	76-77	-0.05421180327989816
1104	78-79	0.0032450406036304003
1104	80-81	0.04277062940463594
1104	82-83	0.0020461480532247833
1104	84-85	0.0025570283567404317
1104	86-87	-0.005619524325183534
1104	88-89	0.002701841850345943
1104	90-91	-0.00719208954486561
1104	92-93	-0.028164298694562717
1104	94-95	-0.04555873145515932
1104	96-97	-0.005460399012719108
1104	98-99	0.030878750965168678
1104	100	0.06694701589695029
1105	1	-0.19983856365339392
1105	2	-0.202638535465141
1105	3	-0.20039817075414135
1105	4	-0.20645938019902843
1105	5	-0.20383814969434866
1105	6	-0.1924521778371755
1105	7	-0.23583404079164438
1105	8	-0.172247760427922
1105	9	-0.17313374669962656
1105	10-11	-0.23880472417874188
1105	12-13	-0.25811085794232724
1105	14-15	-0.2549158712757489
1105	16-17	-0.22496475814114092
1105	18-19	-0.20393981935854555
1105	20-21	-0.22907715326612532
1105	22-23	-0.1671369498283113
1105	24-25	-0.16149948163049999
1105	26-27	-0.16978959283594008
1105	28-29	-0.14255614208403244
1105	30-31	-0.13374277922928002
1105	32-33	-0.136304204366823
1105	34-35	-0.18160459483803493
1105	36-37	-0.12372161382641167
1105	38-39	-0.053906996752438374
1105	40-41	-0.07365959979283332
1105	42-43	-0.0879442993093349
1105	44-45	-0.14283636138240752
1105	46-47	-0.13326056919691354
1105	48-49	-0.1616993968446394
1105	50-51	-0.03362167059265175
1105	52-53	-0.027812068951895697
1105	54-55	-0.11712379710153442
1105	56-57	-0.08392147610221201
1105	58-59	-0.06728923505131945
1105	60-61	-0.07041064487970772
1105	62-63	-0.10483260945377282
1105	64-65	-0.12249678058831392
1105	66-67	-0.10251572508538231
1105	68-69	-0.10133110136847989
1105	70-71	-0.11028535474764567
1105	72-73	-0.10496153736929159
1105	74-75	-0.04562099349664095
1105	76-77	-0.05116998509387116
1105	78-79	-0.028838047558963353
1105	80-81	-0.013609277487141469
1105	82-83	0.011177619120573468
1105	84-85	0.016397123012069414
1105	86-87	0.001725749256330289
1105	88-89	-0.00727477165356305
1105	90-91	-0.008445411795754865
1105	92-93	-0.009723034471591063
1105	94-95	-0.021479825654090234
1105	96-97	0.028821851238305385
1105	98-99	0.02755715634378575
1105	100	0.08538257406631189
1106	1	-0.16202785901680983
1106	2	-0.16572555542798995
1106	3	-0.18249586763965198
1106	4	-0.116057213836541
1106	5	-0.1796548291057931
1106	6	-0.1841340621844978
1106	7	-0.19378318004002182
1106	8	-0.12371336679682088
1106	9	-0.06135649743453797
1106	10-11	-0.158660558623005
1106	12-13	-0.17287951695886505
1106	14-15	-0.16736190315737787
1106	16-17	-0.1522429716323117
1106	18-19	-0.13576181660992148
1106	20-21	-0.18468637414478195
1106	22-23	-0.16113025056176156
1106	24-25	-0.15521354602714155
1106	26-27	-0.15376539554821989
1106	28-29	-0.13267143961481764
1106	30-31	-0.13917289299111957
1106	32-33	-0.12035840622193916
1106	34-35	-0.09612764302973886
1106	36-37	-0.1031252129545166
1106	38-39	-0.06794296333166017
1106	40-41	-0.06458252446115864
1106	42-43	-0.09179363351537972
1106	44-45	-0.1435408377785734
1106	46-47	-0.10264407352080696
1106	48-49	-0.11999094914425257
1106	50-51	-0.004540499320611957
1106	52-53	-0.027391962522500535
1106	54-55	-0.03862885074641298
1106	56-57	-0.07072234912289588
1106	58-59	-0.08074646246603834
1106	60-61	-0.09430435614151378
1106	62-63	-0.11119105497079573
1106	64-65	-0.13657678338206125
1106	66-67	-0.17451401457037719
1106	68-69	-0.17039807669567608
1106	70-71	-0.12941550928100298
1106	72-73	-0.10578224203015196
1106	74-75	-0.05947756183111608
1106	76-77	-0.06033002952940336
1106	78-79	-0.06034527012377566
1106	80-81	-0.0458277081502203
1106	82-83	-0.006765524737616602
1106	84-85	0.01432970860361138
1106	86-87	-0.03505122494235735
1106	88-89	-0.04963307306528719
1106	90-91	-0.045060877672298716
1106	92-93	-0.04808866814268242
1106	94-95	-0.09468291539437246
1106	96-97	0.003262086806557818
1106	98-99	-0.03492339665710986
1106	100	0.006656623117528682
1107	1	-0.13204107820366318
1107	2	-0.14598576920565165
1107	3	-0.1444321160124744
1107	4	-0.11297131002331184
1107	5	-0.13337933550151604
1107	6	-0.13319472121597187
1107	7	-0.11132915018457368
1107	8	-0.1062898159812633
1107	9	-0.06694081157700538
1107	10-11	-0.14849474560887188
1107	12-13	-0.19010977020838027
1107	14-15	-0.14885577397149774
1107	16-17	-0.1581359573124672
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2316	90-91	-0.1824035636732546
2316	92-93	0.03271522268825322
2316	94-95	0.00465575598529
2316	96-97	-0.04232917621418153
2316	98-99	0.11966177251365195
2316	100	-0.20176922497156724
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1507.0
3	221.0
4	183.0
5	181.0
6	729.0
7	6100.0
8	13521.0
9	18048.0
10	22402.0
11	25301.0
12	27195.0
13	28225.0
14	32267.0
15	40185.0
16	50175.0
17	59895.0
18	64328.0
19	70891.0
20	80034.0
21	97118.0
22	121535.0
23	149636.0
24	220906.0
25	305552.0
26	340483.0
27	335062.0
28	263644.0
29	255654.0
30	280745.0
31	328831.0
32	416680.0
33	553975.0
34	801541.0
35	1382609.0
36	2693877.0
37	6081176.0
38	7985867.0
39	2377362.0
40	5500.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	17.595670989566443	30.509538895913536	28.315126702171163	23.579663412348857
2	23.11895701168212	31.787946956184033	25.00754669648209	20.085549335651752
3	24.778588195680843	25.377794519867177	26.765252753355956	23.078364531096028
4	20.886221902265305	27.561495441338614	24.355443304264966	27.196839352131114
5	23.017190206838745	29.509659831280437	25.931972838155996	21.541177123724818
6	27.572417861790765	27.44029792766972	24.769925331198017	20.217358879341496
7	22.942052969366465	25.728146440396245	29.06401580881382	22.26578478142347
8	26.06085855625318	27.481013747565402	24.665776424844886	21.792351271336532
9	25.09569857640155	26.06561517788528	26.462587775672315	22.376098470040855
10-11	22.23601506091301	28.80867824035354	27.068393493865422	21.886913204868026
12-13	22.02828910015451	26.914646782172035	27.18822005978726	23.8688440578862
14-15	22.041808525552614	30.215004812824592	25.84511085160758	21.898075810015218
16-17	23.852264262204248	26.57588511416449	25.747686063006224	23.82416456062504
18-19	22.099439659628864	28.260645997098827	26.015148928037696	23.624765415234616
20-21	23.611161271155677	28.598674822218843	25.95472971360307	21.83543419302241
22-23	24.02583329015477	26.657553031006497	27.413187990208694	21.90342568863004
24-25	22.215881240486688	28.296640802483473	25.662437582309593	23.825040374720245
26-27	22.069274004490012	26.872346072733365	29.13148812876528	21.92689179401134
28-29	22.30715039933821	26.709897864886965	29.084866633569007	21.89808510220582
30-31	22.23849376651958	26.408313697261388	29.342520219656237	22.010672316562793
32-33	22.342484599909458	26.560048807278026	29.114804276206286	21.98266231660623
34-35	22.358379796784554	26.912985020956448	28.801473365519197	21.9271618167398
36-37	22.06390268124209	26.853936036689248	29.127732909041793	21.95442837302687
38-39	22.130133284303017	26.646979107587356	29.148742932102493	22.074144676007133
40-41	22.387519063705433	26.66801600640372	28.882907308498464	22.06155762139238
42-43	22.420852194847196	26.68582051847883	29.02103892530429	21.872288361369684
44-45	22.68271182232499	26.769346289586494	28.770283854047474	21.777658034041043
46-47	22.329952103686445	26.842077160606998	28.87137122667276	21.956599509033797
48-49	22.58701344685171	26.840885808394454	28.60244629572655	21.96965444902728
50-51	22.687944417793474	26.884542180023203	28.52400912211386	21.903504280069466
52-53	22.631547396313177	27.18092188231559	28.5096988515889	21.677831869782334
54-55	22.863826207704278	27.11912085415498	28.064120379210966	21.95293255892978
56-57	23.192067205125838	27.65247711816198	27.45771598503371	21.697739691678475
58-59	23.3703918427145	27.91640388434705	27.080961763703527	21.63224250923492
60-61	23.505166669101797	28.23766877640147	26.71024226964058	21.54692228485615
62-63	23.61964322722479	28.346063680718576	26.596004328404945	21.438288763651695
64-65	23.77519294258082	28.391842129596363	26.528026954289924	21.304937973532894
66-67	23.86893292656713	28.25490376613634	26.65909008693148	21.217073220365048
68-69	24.01594809313195	28.162929830442902	26.756121206385288	21.065000870039864
70-71	24.18608916655693	28.00785273599121	26.834964540678268	20.97109355677359
72-73	24.356115808509937	27.90431980589162	26.823701843489385	20.91586254210906
74-75	24.586692037776942	27.908062634047003	26.742821133476685	20.76242419469937
76-77	24.825925584714184	27.929849205080426	26.544454807254702	20.699770402950687
78-79	25.033833758715573	27.953868368036318	26.358563038208366	20.65373483503975
80-81	25.256011089011665	28.006568930184834	26.16456384144011	20.572856139363395
82-83	25.452108928829965	28.006634882016385	25.979517680348025	20.561738508805625
84-85	25.591892030600576	27.9620928649979	25.912939896987307	20.53307520741422
86-87	25.725524173483155	27.94942562400262	25.843783816522492	20.481266385991734
88-89	25.836409066466594	27.950802635587085	25.77506222152135	20.437726076424973
90-91	25.934867691771498	27.893719714779742	25.749392326891424	20.422020266557343
92-93	26.02870900546111	27.946283571788378	25.699436576024244	20.325570846726272
94-95	26.073768907381755	28.00343048683438	25.640714321512547	20.282086284271315
96-97	26.078128625279728	28.08716077175677	25.61207919087356	20.222631412089946
98-99	26.147492865022524	28.205280516200794	25.515497768623916	20.131728850152765
100	26.08489537036921	28.34136553233796	25.448516247650833	20.125222849642
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	88495.0
1	53761.0
2	15462.5
3	10964.0
4	9632.5
5	8863.5
6	8337.0
7	8091.0
8	7892.0
9	7875.0
10	8101.0
11	11265.5
12	88490.0
13	101968.5
14	39411.5
15	39788.0
16	44354.0
17	48786.0
18	52549.0
19	56956.0
20	63473.0
21	73965.5
22	89521.5
23	107633.5
24	126779.0
25	148370.0
26	171303.5
27	199409.0
28	236094.5
29	272061.5
30	304259.0
31	339601.5
32	376677.5
33	408999.0
34	440049.5
35	480040.5
36	525271.0
37	562864.5
38	600550.0
39	637388.0
40	665499.0
41	698726.0
42	740014.5
43	767257.0
44	787459.5
45	818397.5
46	853034.5
47	885895.5
48	913764.5
49	935328.0
50	948892.5
51	954949.5
52	952375.0
53	938705.5
54	911208.5
55	870382.0
56	819998.0
57	758503.0
58	683597.0
59	592938.0
60	491942.5
61	398213.0
62	316830.5
63	242861.0
64	181956.0
65	135857.5
66	99781.5
67	74523.5
68	56593.5
69	43334.0
70	34512.0
71	29300.5
72	25495.0
73	20310.5
74	15489.0
75	12441.5
76	9481.5
77	6205.0
78	4128.5
79	2902.0
80	2027.0
81	1505.5
82	1115.5
83	816.5
84	638.5
85	503.5
86	419.0
87	353.5
88	280.0
89	230.0
90	190.0
91	164.0
92	147.5
93	127.0
94	99.0
95	82.5
96	74.5
97	57.5
98	39.0
99	31.5
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.039081189144145455
2	0.04469609999803831
3	0.01577970065633766
4	0.04191213792194498
5	0.05970443563469892
6	0.060150809300907965
7	0.043474445753676684
8	0.03816886401934975
9	0.02800407421690495
10-11	0.022561447935934887
12-13	0.015407722601163446
14-15	0.012533702680133213
16-17	0.019820557003072264
18-19	0.018250418054389535
20-21	0.25500074571811165
22-23	0.21099182623252674
24-25	0.19135921603627937
26-27	0.17790927267287493
28-29	0.17972609180551533
30-31	0.17383121852062292
32-33	0.1681477853934085
34-35	0.1596921368655273
36-37	0.15665170570928758
38-39	0.16441430038700205
40-41	0.16727461585336798
42-43	0.10349212606641704
44-45	0.077641609011047
46-47	0.048290582678563856
48-49	0.03087809413793518
50-51	0.03732310338863786
52-53	0.032332724111590126
54-55	0.038435121995684975
56-57	0.04085297935431736
58-59	0.04323168112819456
60-61	0.039249558158592726
62-63	0.044999555779890954
64-65	0.042622811785251506
66-67	0.04100177057638704
68-69	0.045322589354121194
70-71	0.04893077648931106
72-73	0.05328096195561158
74-75	0.05499793434712624
76-77	0.05732573386082171
78-79	0.048911198696933465
80-81	0.049375192376282344
82-83	0.05617455966901941
84-85	0.05533075681754527
86-87	0.052965759498332377
88-89	0.06036224945858594
90-91	0.05766442966895402
92-93	0.03728003224540716
94-95	0.07497511368921922
96-97	0.05126836489919532
98-99	0.23457523493057184
100	0.05953606662025164
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.5539141E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.09286648554115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.79158472523558	37.69919016129112
2	11.409995092678857	11.202987313711258
3	4.278731895016217	6.301656409483729
4	2.1786922035483167	4.278329818475479
5	1.303415307604247	3.199419683571292
6	0.861484109841415	2.5375634630315913
7	0.5537573456249354	1.9029874803906832
8	0.3940737755708936	1.547696899964399
9	0.30119029768340555	1.3307665563820639
>10	1.6900900235836858	15.329117576063075
>50	0.1715466624934681	5.881056055592843
>100	0.06457520075821788	4.5246319995145
>500	5.068435998966831E-4	0.17393812196897254
>1k	3.161805291423328E-4	0.26385318549333436
>5k	0.0	0.0
>10k+	4.033623181315577E-5	3.8268052750656043
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	749671	2.9353806379000766	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	95435	0.3736813231110631	Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	81827	0.3203984033762138	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.41560129214995917	0.0	0.0	0.0	0.0
2	0.4156404477347143	1.1746675426554088E-5	3.91555847551803E-6	0.0	1.566223390207212E-5
3	0.4156482788516654	2.3493350853108177E-5	3.91555847551803E-6	3.91555847551803E-6	1.9577792377590146E-5
4	0.4156952655533716	3.5240026279662265E-5	3.91555847551803E-6	7.83111695103606E-6	2.7408909328626204E-5
5	0.4157030966703226	5.873337713277044E-5	3.91555847551803E-6	7.83111695103606E-6	2.7408909328626204E-5
6	0.41570701222879813	6.264893560828848E-5	3.91555847551803E-6	7.83111695103606E-6	3.132446780414424E-5
7	0.4157187589042247	6.65644940838065E-5	3.91555847551803E-6	7.83111695103606E-6	3.5240026279662265E-5
8	0.41572659002117573	7.831116951036058E-5	3.91555847551803E-6	7.83111695103606E-6	4.6986701706216354E-5
9	0.41576183004745537	9.788896188795073E-5	3.91555847551803E-6	7.83111695103606E-6	5.481781865725241E-5
10-11	0.4157951122944973	1.1355119579002285E-4	3.91555847551803E-6	1.1746675426554088E-5	5.873337713277044E-5
12-13	0.4158186056453504	1.2334009197881792E-4	3.91555847551803E-6	1.7620013139831133E-5	6.069115637052946E-5
14-15	0.4158597190093434	1.448756635941671E-4	3.91555847551803E-6	2.3493350853108177E-5	6.852227332156551E-5
16-17	0.41590279015257403	1.5858011825848017E-4	3.91555847551803E-6	2.3493350853108177E-5	7.048005255932453E-5
18-19	0.41593803017885367	1.762001313983113E-4	3.91555847551803E-6	2.545113009086719E-5	8.222672798587862E-5
20-21	0.4160613702708325	2.0948237844021456E-4	3.91555847551803E-6	2.7408909328626204E-5	1.0180452036346876E-4
22-23	0.4164862083654262	2.3493350853108177E-4	3.91555847551803E-6	2.7408909328626204E-5	1.0767785807674581E-4
24-25	0.4181659829514235	2.5842685938418997E-4	3.91555847551803E-6	2.936668856638522E-5	1.2138231274105892E-4
26-27	0.4239649250536657	2.7408909328626205E-4	3.91555847551803E-6	3.132446780414424E-5	1.2529787121657695E-4
28-29	0.43914554526324906	3.1911801575471943E-4	3.91555847551803E-6	3.328224704190325E-5	1.2529787121657695E-4
30-31	0.4734419219503115	3.524002627966227E-4	3.91555847551803E-6	3.5240026279662265E-5	1.3117120892985396E-4
32-33	0.5371911294902205	3.700202759364538E-4	3.91555847551803E-6	3.915558475518029E-5	1.3704454664313103E-4
34-35	0.6380774513911803	3.837247306007669E-4	3.91555847551803E-6	4.307114323069832E-5	1.4096010511864906E-4
36-37	0.779836330438835	4.05260302216116E-4	3.91555847551803E-6	4.502892246845734E-5	1.5858011825848017E-4
38-39	0.9625010488802266	4.3071143230698325E-4	3.91555847551803E-6	4.6986701706216354E-5	1.6249567673399823E-4
40-41	1.189192306820343	4.463736662090554E-4	3.91555847551803E-6	4.6986701706216354E-5	1.7032679368503428E-4
42-43	1.4650218658489726	4.5812034163560946E-4	3.91555847551803E-6	4.6986701706216354E-5	1.742423521605523E-4
44-45	1.7899916837453538	4.7769813401319955E-4	3.91555847551803E-6	4.6986701706216354E-5	1.9186236530038342E-4
46-47	2.1624983393137613	4.953181471530307E-4	3.91555847551803E-6	5.090226018173438E-5	2.0752459920245556E-4
48-49	2.5800025928828223	5.22727056481657E-4	3.91555847551803E-6	5.090226018173438E-5	2.271023915800457E-4
50-51	3.041455074781098	5.403470696214881E-4	3.91555847551803E-6	5.090226018173438E-5	2.3101795005556373E-4
52-53	3.5468029249691675	5.520937450480422E-4	3.91555847551803E-6	5.090226018173438E-5	2.3689128776884077E-4
54-55	4.093360070332828	5.814604336144274E-4	3.91555847551803E-6	5.090226018173438E-5	2.4668018395763584E-4
56-57	4.684116039768135	6.029960052297765E-4	3.91555847551803E-6	5.090226018173438E-5	2.545113009086719E-4
58-59	5.309434643866839	6.127849014185716E-4	3.91555847551803E-6	5.090226018173438E-5	2.68215755572985E-4
60-61	5.965089037254621	6.323626937961618E-4	3.91555847551803E-6	5.090226018173438E-5	2.9758244413937026E-4
62-63	6.651907360549049	6.499827069359929E-4	7.83111695103606E-6	5.090226018173438E-5	3.1520245727920137E-4
64-65	7.371019252370313	6.71518278551342E-4	7.83111695103606E-6	5.090226018173438E-5	3.230335742302374E-4
66-67	8.112810058881777	6.910960709289322E-4	7.83111695103606E-6	5.090226018173438E-5	3.308646911812735E-4
68-69	8.876982197639302	7.165472010197994E-4	7.83111695103606E-6	5.481781865725241E-5	3.4065358737006855E-4
70-71	9.659580954582616	7.380827726351485E-4	7.83111695103606E-6	5.481781865725241E-5	3.426113666078276E-4
72-73	10.461493203706421	7.439561103484255E-4	7.83111695103606E-6	5.6775597895011425E-5	3.5435804203438166E-4
74-75	11.280312834327514	7.654916819637747E-4	7.83111695103606E-6	5.873337713277044E-5	3.582736005098997E-4
76-77	12.11724779623559	7.88985032816883E-4	7.83111695103606E-6	6.852227332156551E-5	3.6414693822317675E-4
78-79	12.968243528629252	8.10520604432232E-4	7.83111695103606E-6	7.048005255932453E-5	3.6806249669869476E-4
80-81	13.830052075753057	8.340139552853402E-4	7.83111695103606E-6	7.831116951036058E-5	3.7589361364973083E-4
82-83	14.69800413412495	8.594650853762074E-4	7.83111695103606E-6	7.831116951036058E-5	3.9547140602732097E-4
84-85	15.581119192693286	8.888317739425926E-4	7.83111695103606E-6	8.02689487481196E-5	4.111336399293931E-4
86-87	16.47430702543989	9.025362286069058E-4	7.83111695103606E-6	8.222672798587862E-5	4.189647568804292E-4
88	17.147456134096288	9.084095663201828E-4	7.83111695103606E-6	8.222672798587862E-5	4.267958738314652E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	101315	0.0	81.39001	8
GTGGTAT	103720	0.0	79.846275	6
TGGTATC	103410	0.0	79.831764	7
GTATCAA	103405	0.0	79.547844	9
AGTGGTA	104615	0.0	79.25534	5
AAGCAGT	110945	0.0	75.38216	1
GCAGTGG	114295	0.0	73.184166	3
CAGTGGT	113930	0.0	73.05988	4
AGCAGTG	121255	0.0	69.32932	2
CAACGCA	98500	0.0	43.825718	12-13
ATCAACG	100060	0.0	43.31918	10-11
ACGCAGA	102110	0.0	42.499287	14-15
TCAACGC	97935	0.0	41.810863	12-13
AGAGTAC	103400	0.0	41.70954	18-19
AACGCAG	100545	0.0	40.77546	14-15
GAGTACT	97530	0.0	40.43174	20-21
AGTACTT	104365	0.0	39.905464	20-21
CGCAGAG	104415	0.0	39.282356	16-17
TATCAAC	105020	0.0	39.205452	10-11
GTACTTT	103135	0.0	38.12543	22-23
>>END_MODULE