FastQCFastQC Report
Thu 26 May 2016
SRR1660677_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660677_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25539141
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9971943.904571418435726No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTCATCTCGTATG733120.2870574229571778TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460130.18016659213401107No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA387150.1515908463796805No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT307540.12041908535608148No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1311100.082.388288
GTGGTAT1324450.081.6619956
TGGTATC1326650.081.3198857
GTATCAA1327500.081.143949
AGTGGTA1334900.081.124795
AAGCAGT1382000.079.0108261
GCAGTGG1424850.076.4266743
CAGTGGT1423100.076.384124
AGCAGTG1501400.072.8551942
CAACGCA1275500.044.27440612-13
ATCAACG1294450.043.7148410-11
ACGCAGA1314050.043.04057314-15
AGAGTAC1328200.042.38720318-19
TCAACGC1273150.042.10132612-13
AACGCAG1296250.041.23571414-15
AGTACTT1347000.040.9470120-21
GAGTACT1272300.040.84895320-21
TATCAAC1344350.040.00403210-11
CGCAGAG1338350.039.86719516-17
GCAGAGT1452250.039.00837716-17
Produced by FastQC (version 0.11.5)