##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660677_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25539141
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.978108582430394	34.0	33.0	34.0	31.0	34.0
2	33.14500859680441	34.0	33.0	34.0	31.0	34.0
3	33.14796112367288	34.0	33.0	34.0	31.0	34.0
4	36.49073741360369	37.0	37.0	37.0	35.0	37.0
5	36.4319483572294	37.0	37.0	37.0	35.0	37.0
6	36.42618230581835	37.0	37.0	37.0	35.0	37.0
7	36.369702841610845	37.0	37.0	37.0	35.0	37.0
8	36.400856982621306	37.0	37.0	37.0	35.0	37.0
9	38.225139248027176	39.0	39.0	39.0	37.0	39.0
10-11	38.15892993425268	39.0	39.0	39.0	37.0	39.0
12-13	38.21966780323582	39.0	39.0	39.0	37.0	39.0
14-15	39.809711141028586	41.0	40.0	41.0	38.0	41.0
16-17	39.772543367844676	41.0	40.0	41.0	37.5	41.0
18-19	39.74854980439632	41.0	40.0	41.0	37.0	41.0
20-21	39.710860204734374	41.0	40.0	41.0	37.0	41.0
22-23	39.59608739385557	41.0	39.5	41.0	37.0	41.0
24-25	39.60505245654112	41.0	39.5	41.0	37.0	41.0
26-27	39.579654499734346	41.0	40.0	41.0	37.0	41.0
28-29	39.50998637738051	41.0	40.0	41.0	37.0	41.0
30-31	39.44981156570614	41.0	39.5	41.0	36.5	41.0
32-33	39.36756561232815	41.0	39.0	41.0	36.0	41.0
34-35	39.2948485033228	41.0	39.0	41.0	36.0	41.0
36-37	39.15180536025076	40.5	39.0	41.0	35.5	41.0
38-39	38.839764677284954	40.0	38.0	41.0	35.0	41.0
40-41	38.74915358351325	40.0	38.0	41.0	35.0	41.0
42-43	38.63369977870438	40.0	38.0	41.0	35.0	41.0
44-45	38.540671160396506	40.0	38.0	41.0	35.0	41.0
46-47	38.47036513483363	40.0	38.0	41.0	35.0	41.0
48-49	38.43752419080971	40.0	38.0	41.0	35.0	41.0
50-51	38.39285465787593	40.0	37.5	41.0	35.0	41.0
52-53	38.16248512430391	40.0	37.0	41.0	34.0	41.0
54-55	37.61179823941612	40.0	37.0	41.0	33.0	41.0
56-57	37.23648489195467	40.0	36.0	41.0	33.0	41.0
58-59	37.002922083401316	39.5	36.0	41.0	32.0	41.0
60-61	36.726437059883885	39.0	35.0	41.0	32.0	41.0
62-63	36.51065431292305	39.0	35.0	41.0	31.5	41.0
64-65	36.2122902450008	38.0	35.0	40.0	31.0	41.0
66-67	35.942252873736045	37.0	35.0	40.0	31.0	41.0
68-69	35.65038514803611	37.0	35.0	39.5	31.0	41.0
70-71	35.33352503907629	36.0	35.0	39.0	31.0	41.0
72-73	34.97560207682788	36.0	35.0	39.0	30.0	40.5
74-75	34.57182351982786	35.0	35.0	37.5	30.0	40.0
76-77	33.2451299556238	34.5	32.5	36.5	28.0	39.0
78-79	33.92967353522188	35.0	34.0	37.0	30.0	39.0
80-81	33.756428280027116	35.0	34.0	36.0	30.0	39.0
82-83	33.54382402681437	35.0	34.0	36.0	29.5	37.0
84-85	33.34031894416496	35.0	34.0	35.5	29.0	37.0
86-87	33.17102415464952	35.0	34.0	35.0	29.0	36.5
88-89	33.01972466497601	35.0	34.0	35.0	29.0	36.0
90-91	32.89723693134393	35.0	34.0	35.0	29.0	36.0
92-93	32.772521205783704	35.0	34.0	35.0	29.0	36.0
94-95	32.69732821084311	35.0	34.0	35.0	29.0	35.5
96-97	32.61470736231888	35.0	34.0	35.0	29.0	35.0
98-99	32.52482370100075	35.0	34.0	35.0	27.0	35.0
100	32.498863763663785	35.0	34.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.08173741080700125
1101	2	0.07101207451012215
1101	3	0.07859632012713291
1101	4	0.05468802790731786
1101	5	0.031089281187327344
1101	6	0.05184884692476288
1101	7	0.061557878315809944
1101	8	0.04782154553271312
1101	9	0.0599046558744476
1101	10-11	0.06090458559049239
1101	12-13	0.0669931536752344
1101	14-15	0.04560200831711825
1101	16-17	0.05540144113609102
1101	18-19	0.04026399320787277
1101	20-21	0.042511730824493554
1101	22-23	0.07949324061500107
1101	24-25	0.08417224618870733
1101	26-27	0.09128777952481215
1101	28-29	0.10644753924771067
1101	30-31	0.0796917080028905
1101	32-33	0.11870260019226464
1101	34-35	0.12536192832310178
1101	36-37	0.11138621195159715
1101	38-39	0.11436992130850143
1101	40-41	0.08951983454962686
1101	42-43	0.12274825111811793
1101	44-45	0.13944504824961967
1101	46-47	0.09813174509974942
1101	48-49	0.12246177064881891
1101	50-51	0.06671374929940299
1101	52-53	0.07207146770539907
1101	54-55	0.09861310615617214
1101	56-57	0.037073495812627755
1101	58-59	0.005650445931962622
1101	60-61	0.0069136419265731774
1101	62-63	-0.012663910032337355
1101	64-65	-0.013760260444534822
1101	66-67	-0.0137996408566039
1101	68-69	0.006329356461726832
1101	70-71	0.01812840125312931
1101	72-73	-0.005468271760456389
1101	74-75	-0.0021761628781220566
1101	76-77	0.1706728718260564
1101	78-79	0.012336383420688435
1101	80-81	0.04129383010592136
1101	82-83	0.02589375748358691
1101	84-85	-0.00547437111975313
1101	86-87	-0.012658810829663025
1101	88-89	0.04439424464871422
1101	90-91	0.0238107647945327
1101	92-93	0.037656215268278004
1101	94-95	0.055109030975799556
1101	96-97	0.0419689592324346
1101	98-99	0.03553215639878715
1101	100	0.037525026939455586
1102	1	0.07467440765227451
1102	2	0.06543522268479762
1102	3	0.05988068409602221
1102	4	0.049307159330020056
1102	5	0.046838657135985784
1102	6	0.04339410475105865
1102	7	0.056315171832800104
1102	8	0.05121305486900951
1102	9	0.04381363289575546
1102	10-11	0.033655344697244516
1102	12-13	0.022253088126412024
1102	14-15	0.041551227989792494
1102	16-17	0.07427551148668243
1102	18-19	0.046904010866327894
1102	20-21	0.049220716894346594
1102	22-23	0.06645003114994097
1102	24-25	0.0695962343197607
1102	26-27	0.08090701694290914
1102	28-29	0.06939358367314696
1102	30-31	0.0977556446679202
1102	32-33	0.04659024840326964
1102	34-35	0.07633580699298648
1102	36-37	0.12283036195721309
1102	38-39	0.1368801406758493
1102	40-41	0.13021511007858777
1102	42-43	0.11123147829700031
1102	44-45	0.10645002252967117
1102	46-47	0.1186778709692291
1102	48-49	0.08712537837802614
1102	50-51	0.07046531535217326
1102	52-53	0.08498536323954653
1102	54-55	0.12809734717748
1102	56-57	0.09006556361490681
1102	58-59	0.06474833466433694
1102	60-61	0.07788832927691658
1102	62-63	0.018125587842796165
1102	64-65	0.02214718252800907
1102	66-67	0.03073157078604538
1102	68-69	0.0098383088050511
1102	70-71	0.05307443558404401
1102	72-73	0.04160542822673108
1102	74-75	0.04804839981242992
1102	76-77	0.1740734347203059
1102	78-79	0.09319069116613576
1102	80-81	0.12667305502588277
1102	82-83	0.06891705185974928
1102	84-85	0.07485858626148456
1102	86-87	0.04237365345642985
1102	88-89	0.0430397711284769
1102	90-91	0.03593392060399481
1102	92-93	0.06195010039687787
1102	94-95	0.07532386715032402
1102	96-97	0.10138538184590828
1102	98-99	0.0821285672103329
1102	100	0.048523882209885016
1103	1	0.026439336163775806
1103	2	0.006250141143553378
1103	3	0.004969663983715122
1103	4	5.206430311943677E-4
1103	5	0.016715502266677618
1103	6	0.0035436103974078037
1103	7	-0.006548594287217213
1103	8	-0.00739336373442967
1103	9	0.006929061387957347
1103	10-11	-0.0051977635584208315
1103	12-13	0.027632049138603065
1103	14-15	0.027795789629649903
1103	16-17	0.023927886363011908
1103	18-19	0.0060600799493997215
1103	20-21	0.04182749527272733
1103	22-23	0.03339439450126491
1103	24-25	-0.004852087884295031
1103	26-27	-0.02630199035130687
1103	28-29	0.02689111231818231
1103	30-31	0.03456154286611479
1103	32-33	-0.010050882566609687
1103	34-35	0.025855511792428842
1103	36-37	0.029809475805734564
1103	38-39	0.05911143969591848
1103	40-41	0.09081379427900771
1103	42-43	0.04076388355115057
1103	44-45	0.041817416834298626
1103	46-47	0.04405274593094077
1103	48-49	0.06862912331610715
1103	50-51	-0.002423254263966612
1103	52-53	0.024728238452468077
1103	54-55	-0.008466777887569776
1103	56-57	-0.010510024163743026
1103	58-59	-0.03699146401786635
1103	60-61	0.009317567744652422
1103	62-63	-0.04157181741619098
1103	64-65	-0.020935360968778127
1103	66-67	-0.056451688413261536
1103	68-69	-0.06135235883765233
1103	70-71	-0.08149669934964976
1103	72-73	-0.06583909385728504
1103	74-75	-0.03724622353118434
1103	76-77	0.13186707462490688
1103	78-79	0.09727403892394904
1103	80-81	0.07185945467886512
1103	82-83	0.0372071395373581
1103	84-85	0.017744163458665696
1103	86-87	0.04748714398785836
1103	88-89	0.023165674851640006
1103	90-91	0.015571972113235688
1103	92-93	0.07575154208981161
1103	94-95	0.05611787366261467
1103	96-97	0.03032685251815792
1103	98-99	0.06395464352349478
1103	100	-0.018424612994529355
1104	1	-0.023698764068349476
1104	2	-0.014246349210992548
1104	3	-0.028755855088185456
1104	4	-0.01707823054371005
1104	5	-0.0015541882486544978
1104	6	-0.0031219539292166587
1104	7	-0.01936564748469749
1104	8	-0.028168711954215553
1104	9	-0.03723306066136445
1104	10-11	-0.03775414669785704
1104	12-13	-0.0359124067035026
1104	14-15	-0.05730604789169291
1104	16-17	-0.02187360331642907
1104	18-19	-0.024145246114130714
1104	20-21	-0.0410046521916243
1104	22-23	-0.019888926247510597
1104	24-25	-0.027705726646296114
1104	26-27	-0.022347736959737574
1104	28-29	0.014834718920937462
1104	30-31	0.02292262957688962
1104	32-33	-0.006274160868123602
1104	34-35	-0.021842298954176442
1104	36-37	-0.023415119897350678
1104	38-39	0.001462225882086443
1104	40-41	0.004094729306743261
1104	42-43	-0.030452109155646667
1104	44-45	-0.022555547680589427
1104	46-47	-0.019514882653382415
1104	48-49	-0.02579256670145469
1104	50-51	-0.07550535371643008
1104	52-53	-0.1046256588052259
1104	54-55	-0.05779443403142892
1104	56-57	-0.06251081867883101
1104	58-59	-0.09253415446170266
1104	60-61	-0.056634695310592065
1104	62-63	-0.1186983259181531
1104	64-65	-0.0820804070744785
1104	66-67	-0.1018589688467344
1104	68-69	-0.12500988691470383
1104	70-71	-0.11536917031324379
1104	72-73	-0.13041896016110144
1104	74-75	-0.1277659270743925
1104	76-77	-0.005070839245924219
1104	78-79	-0.07160598450711575
1104	80-81	-0.037642237958614544
1104	82-83	-0.05210555974689157
1104	84-85	-0.07552166972919849
1104	86-87	-0.04695630887561464
1104	88-89	-0.05140470877016412
1104	90-91	-0.07601030923034813
1104	92-93	-0.044183656494574564
1104	94-95	-0.07903198548005008
1104	96-97	-0.05435260417200283
1104	98-99	-0.015123641527949871
1104	100	-0.08027245454344722
1105	1	-0.03957399382589699
1105	2	-0.06033407891047915
1105	3	-0.03870542258487575
1105	4	-0.03215046844838554
1105	5	-0.05723160606448374
1105	6	-0.02779181845608747
1105	7	-0.037597208681553695
1105	8	-0.015177312129267762
1105	9	-0.024533404293208605
1105	10-11	-0.05107425103783925
1105	12-13	-0.06334667271335803
1105	14-15	-0.06519555291989576
1105	16-17	-0.05087644144271053
1105	18-19	-0.08287084679352574
1105	20-21	-0.07288864804222328
1105	22-23	-0.07878012370076704
1105	24-25	-0.07180233590801066
1105	26-27	-0.06900462277842223
1105	28-29	-0.030094003741666597
1105	30-31	-0.04482732958337721
1105	32-33	-0.03518438152158154
1105	34-35	-0.054027040128517
1105	36-37	-0.07378774836452351
1105	38-39	-0.06859899342632758
1105	40-41	-0.1200558301968968
1105	42-43	-0.09548470726144842
1105	44-45	-0.10060217991582476
1105	46-47	-0.06649354868127233
1105	48-49	-0.07238966253337509
1105	50-51	-0.1421491944177049
1105	52-53	-0.13707853466479492
1105	54-55	-0.09934626123417445
1105	56-57	-0.14391282863227417
1105	58-59	-0.1878141615972524
1105	60-61	-0.15705167401717546
1105	62-63	-0.16785555015032116
1105	64-65	-0.1478526721619957
1105	66-67	-0.13242488382242357
1105	68-69	-0.12872829166987998
1105	70-71	-0.17692280780241987
1105	72-73	-0.17623963335213233
1105	74-75	-0.1672899239741099
1105	76-77	-0.004860056696152526
1105	78-79	-0.098811538311665
1105	80-81	-0.06156764196077802
1105	82-83	-0.10491012536881783
1105	84-85	-0.1301273562581784
1105	86-87	-0.13821529781083797
1105	88-89	-0.12948286403897669
1105	90-91	-0.15362992621947313
1105	92-93	-0.09502802602452931
1105	94-95	-0.11274256902909485
1105	96-97	-0.1126539897670753
1105	98-99	-0.07912601848953216
1105	100	-0.08760301279657057
1106	1	-0.08527344923833624
1106	2	-0.10618027950245335
1106	3	-0.08315990774416804
1106	4	-0.04212193215977322
1106	5	-0.048502210081622366
1106	6	-0.0547076982836856
1106	7	-0.04380287769410529
1106	8	-0.044543439933953266
1106	9	-0.05995790101668774
1106	10-11	-0.06513454696898435
1106	12-13	-0.07565412123384618
1106	14-15	-0.12046826115557963
1106	16-17	-0.1059234053213487
1106	18-19	-0.0878782559960456
1106	20-21	-0.08723917825205518
1106	22-23	-0.07093954877256436
1106	24-25	-0.09256249236995728
1106	26-27	-0.06612070260491976
1106	28-29	-0.09725546694470921
1106	30-31	-0.09599927831080635
1106	32-33	-0.07241521898234993
1106	34-35	-0.06436664699741357
1106	36-37	-0.08495413027871734
1106	38-39	-0.061789174353371834
1106	40-41	-0.05801507769265157
1106	42-43	-0.08330430850636361
1106	44-45	-0.10353731786360498
1106	46-47	-0.09616216601822458
1106	48-49	-0.10019233838280428
1106	50-51	-0.12289931040724156
1106	52-53	-0.17089157867964389
1106	54-55	-0.14915756770485444
1106	56-57	-0.17656311791895263
1106	58-59	-0.19632995197159886
1106	60-61	-0.16014509451148484
1106	62-63	-0.17360182561491655
1106	64-65	-0.14485019766346596
1106	66-67	-0.2235635102473239
1106	68-69	-0.1792355656275575
1106	70-71	-0.16213881820308274
1106	72-73	-0.19425899440889793
1106	74-75	-0.21117358288402954
1106	76-77	0.019384228357800737
1106	78-79	-0.09982474231097882
1106	80-81	-0.0881399012599573
1106	82-83	-0.11979847116639064
1106	84-85	-0.12487773979448491
1106	86-87	-0.13851565388649334
1106	88-89	-0.11531609259520792
1106	90-91	-0.18849291777089405
1106	92-93	-0.13003503532515737
1106	94-95	-0.09788611126935365
1106	96-97	-0.11774670356196282
1106	98-99	-0.14563985571938076
1106	100	-0.16610599597971998
1107	1	-0.09867492476818995
1107	2	-0.08171345067653846
1107	3	-0.062380623458160755
1107	4	-0.03631956541532588
1107	5	-0.07253207991987409
1107	6	-0.08082392461525956
1107	7	-0.09961555616355611
1107	8	-0.0703994944499371
1107	9	-0.09847447113271102
1107	10-11	-0.10356518164542905
1107	12-13	-0.08551322477088519
1107	14-15	-0.13429317059009094
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2316	96-97	0.1549625041831959
2316	98-99	0.17543531984727423
2316	100	0.17246160787961884
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	6.0
7	44.0
8	97.0
9	67.0
10	38.0
11	61.0
12	98.0
13	339.0
14	1099.0
15	3207.0
16	7553.0
17	12276.0
18	16311.0
19	21519.0
20	27585.0
21	38342.0
22	58076.0
23	100067.0
24	188303.0
25	286682.0
26	320263.0
27	274890.0
28	233956.0
29	240902.0
30	302430.0
31	328373.0
32	390746.0
33	507704.0
34	728313.0
35	1268301.0
36	2601635.0
37	6341857.0
38	8876642.0
39	2357848.0
40	3511.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	16.926554443699676	30.706905621974993	28.189914793479687	24.176625140845644
2	22.756381375440025	32.15485222420217	25.467738212342	19.621028188015803
3	25.488946666334055	25.040012497284625	26.431647329071573	23.03939350730975
4	20.53675963494622	27.230759249107084	24.32284625391277	27.909634862033926
5	22.90104040695809	30.076904309350105	25.82789687405696	21.19415840963484
6	28.32680099053907	26.869478430503897	24.842994905677127	19.960725673279907
7	22.752143464809567	25.21249637957674	30.033363299102344	22.001996856511344
8	26.75168675406898	26.770003736617454	24.845209163456204	21.633100345857365
9	25.726519149567324	25.311172368718278	26.792549522319486	22.169758959394915
10-11	22.00194986980964	28.649005853407523	27.655975195093678	21.693069081689163
12-13	21.8388453237327	26.243637168532803	27.681643638679937	24.23587386905456
14-15	21.623953601258556	30.835919657595372	25.55285630006115	21.98727044108492
16-17	24.0045035187362	26.120827634727416	25.658644901173457	24.216023945362924
18-19	21.87908487614848	28.18184461203814	25.94723803711958	23.991832474693798
20-21	23.939724076791165	28.298822032782194	25.874112265406644	21.88734162501999
22-23	24.08595374049932	26.296896468416698	27.87985576552226	21.73729402556172
24-25	21.83288755880372	28.18426569760834	25.59186565817827	24.39098108540967
26-27	21.836879122269046	25.81346092867472	30.3690187358667	21.98064121318954
28-29	21.87520662378714	26.088492397468343	30.110146815103274	21.926154163641243
30-31	21.997294164341856	25.77839375522406	30.3769946834678	21.847317396966282
32-33	21.933019520134874	26.161023564302265	29.892198154804085	22.01375876075878
34-35	21.936808706761312	26.06760138605267	29.807807774527305	22.187782132658715
36-37	21.852110026115618	26.23192512453485	29.919032398221567	21.99693245112796
38-39	21.909432715017413	26.027744131794613	30.14228628469632	21.920536868491656
40-41	21.93398319910643	26.29792202648557	29.898872439953887	21.86922233445411
42-43	21.948336149303604	26.099614850463755	30.021090403220313	21.930958597012328
44-45	22.014677782062773	26.17757631456849	29.971586873274482	21.836159030094258
46-47	22.24718235591314	26.287673265564425	29.85854475234288	21.606599626179555
48-49	22.0599132980103	26.530378400972538	29.76616344439756	21.643544856619606
50-51	22.320362229674796	26.418408310877904	29.427004103048937	21.83422535639836
52-53	22.44341173960117	26.527536959783877	29.137874660638456	21.891176639976496
54-55	22.464943618218356	26.784705358527255	28.666692737324656	22.083658285929733
56-57	22.778303912187102	27.230100394438207	28.021533361629793	21.9700623317449
58-59	22.830503427509992	27.554480137950964	27.398909773321854	22.216106661217186
60-61	23.050635461195878	27.68672073356874	27.016492053214996	22.246151752020385
62-63	22.741356287553174	27.8247858477306	27.339777481083104	22.094080383633123
64-65	22.96311360327401	28.0856389036762	26.821583325966337	22.129664167083455
66-67	22.60928221382674	28.164199044314962	26.97128107117262	22.255237670685673
68-69	22.51321085115584	28.094867560897686	27.103222595940796	22.288698992005678
70-71	22.4227795092317	28.012178938354488	27.184809963587643	22.38023158882617
72-73	22.333242355513647	27.948047488072376	27.166747071741167	22.551963084672806
74-75	22.31798222900784	27.9720636385013	27.04496903301924	22.664985099471625
76-77	22.328824163685095	27.958658259253948	26.849115035532765	22.863402541528185
78-79	22.330443427564113	27.990561063820497	26.615977316168145	23.063018192447245
80-81	22.353239455922157	28.047498641763212	26.391129123044017	23.208132779270617
82-83	22.300048261328428	28.08158351348697	26.182129925071894	23.43623830011271
84-85	22.223829559448443	28.022816435804295	26.07728253583858	23.676071468908685
86-87	22.16221393235085	28.03174579929789	25.97466571210284	23.83137455624842
88-89	22.103003859117287	27.961672383482277	25.895738697916908	24.039585059483525
90-91	22.0460920705898	27.840082461793873	25.86841548840797	24.24540997920835
92-93	22.052717374588624	27.77211986477994	25.816803474957272	24.358359285674165
94-95	22.006618381992435	27.69563822169146	25.789153668240576	24.508589728075524
96-97	21.918213025897046	27.596845192822496	25.806118788510595	24.67882299276986
98-99	21.86066229858059	27.57844374814645	25.814777503503088	24.74611644976987
100	21.756376076279548	27.58199803719063	25.78833453463782	24.873291351892
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	70702.0
1	44291.0
2	13018.0
3	7200.0
4	5970.0
5	5587.5
6	5534.0
7	5508.0
8	5385.5
9	5480.0
10	5852.5
11	9820.0
12	108027.5
13	124520.0
14	43457.0
15	42575.0
16	47561.0
17	52823.0
18	58164.5
19	64072.5
20	71643.5
21	83378.0
22	99378.5
23	117289.5
24	136779.5
25	158564.0
26	180716.0
27	207804.0
28	243727.0
29	279544.0
30	310328.5
31	343990.0
32	380287.5
33	410746.0
34	439106.0
35	476276.0
36	520164.5
37	555958.5
38	590792.0
39	627304.5
40	657110.5
41	691996.5
42	735273.5
43	764066.5
44	785024.5
45	815714.5
46	845585.5
47	870148.0
48	890212.0
49	910402.0
50	930568.5
51	949508.5
52	961318.0
53	955254.0
54	929676.5
55	889504.0
56	837619.0
57	771457.0
58	690920.0
59	595167.5
60	490148.0
61	393728.5
62	310839.5
63	235779.5
64	175186.5
65	129911.0
66	94679.0
67	70676.5
68	53095.5
69	39968.5
70	31678.0
71	26735.0
72	23148.5
73	18175.5
74	13493.0
75	10650.0
76	7915.0
77	4960.0
78	2963.5
79	1866.0
80	1152.5
81	757.0
82	465.5
83	260.5
84	159.5
85	100.5
86	66.5
87	34.5
88	13.5
89	6.5
90	6.0
91	5.0
92	3.0
93	2.5
94	2.5
95	1.0
96	1.5
97	1.5
98	1.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08553537489769135
2	0.05635663313813099
3	0.01563874055121901
4	0.0
5	0.0
6	0.0012529787121657694
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	7.576605650127387E-4
20-21	7.341672141596305E-4
22-23	6.69560499313583E-4
24-25	0.0010121718659214106
26-27	0.008451732969405667
28-29	0.008177643876119405
30-31	0.011169130551415178
32-33	0.010225480958815335
34-35	0.012414278146629914
36-37	0.017312641799502967
38-39	0.014295703994116326
40-41	0.02036677741040703
42-43	0.0242803781066873
44-45	0.01679187252225907
46-47	0.017314599578740725
48-49	0.029550719814734568
50-51	0.01464418869843743
52-53	0.02382421554428945
54-55	0.07342455253291409
56-57	0.0646537015477537
58-59	0.058731419353532675
60-61	0.09648131861600201
62-63	0.05124095598986669
64-65	0.03374428294201438
66-67	0.042507302810223725
68-69	0.03557872208779458
70-71	0.017240203967705883
72-73	0.0069736096448976105
74-75	0.00570105314035425
76-77	0.003461353692357938
78-79	9.632273849774353E-4
80-81	0.002020428173367303
82-83	2.3297572929332277E-4
84-85	2.936668856638522E-5
86-87	3.0149800261488827E-4
88-89	4.111336399293931E-5
90-91	1.605378974962392E-4
92-93	7.028427463554863E-4
94-95	4.6790923782440456E-4
96-97	5.775448751389093E-4
98-99	0.0010454541129633137
100	9.279873586977729E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.5539141E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.97911307299681
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.40477900208691	36.6583353105846
2	11.634925809555305	11.164668420251658
3	4.194689897678749	6.037725027206583
4	2.2181184648727466	4.256934265417264
5	1.364800895249409	3.2740968237649333
6	0.8809471285353403	2.5360237134797368
7	0.5809662062616426	1.9511970311272138
8	0.41682168098784433	1.599898765071391
9	0.31651835698571995	1.3667643035547323
>10	1.745788220138762	15.454735240375959
>50	0.17403621831194935	5.847603384824221
>100	0.06672726319680496	4.60715608039247
>500	3.8343524562943645E-4	0.12378185241921152
>1k	4.3238433677487086E-4	0.35605130756679154
>5k	1.6259139139780892E-5	0.040877191543473794
>10k+	4.877741740912022E-5	4.724151282419737
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	997194	3.904571418435726	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTCATCTCGTATG	73312	0.2870574229571778	TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46013	0.18016659213401107	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38715	0.1515908463796805	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30754	0.12041908535608148	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.41820905409465414	1.9577792377590146E-5	0.0	3.91555847551803E-6	3.91555847551803E-6
2	0.41823254744550725	4.307114323069832E-5	0.0	3.91555847551803E-6	7.83111695103606E-6
3	0.4182403785624583	5.481781865725241E-5	0.0	7.83111695103606E-6	7.83111695103606E-6
4	0.418283449705689	7.048005255932453E-5	0.0	7.83111695103606E-6	1.1746675426554088E-5
5	0.41828736526416455	8.222672798587862E-5	0.0	7.83111695103606E-6	1.566223390207212E-5
6	0.4182991119395911	8.222672798587862E-5	0.0	7.83111695103606E-6	1.9577792377590146E-5
7	0.4183030274980666	9.005784493691467E-5	0.0	7.83111695103606E-6	2.3493350853108177E-5
8	0.4183030274980666	9.397340341243271E-5	0.0	7.83111695103606E-6	2.7408909328626204E-5
9	0.4183186897319687	9.788896188795073E-5	0.0	7.83111695103606E-6	3.5240026279662265E-5
10-11	0.4183480564205351	1.0767785807674581E-4	0.0	7.83111695103606E-6	3.5240026279662265E-5
12-13	0.4183872120052902	1.1355119579002285E-4	0.0	7.83111695103606E-6	3.915558475518029E-5
14-15	0.4184753120709894	1.33128988167613E-4	0.0	1.1746675426554088E-5	4.6986701706216354E-5
16-17	0.41853404544812217	1.429178843564081E-4	0.0	1.1746675426554088E-5	5.481781865725241E-5
18-19	0.4185751588121151	1.7032679368503428E-4	0.0	1.1746675426554088E-5	6.460671484604749E-5
20-21	0.41868479444942963	2.1144015767797356E-4	0.0	1.566223390207212E-5	7.243783179708355E-5
22-23	0.419088096972408	2.3101795005556373E-4	0.0	1.566223390207212E-5	8.22267279858786E-5
24-25	0.4208050693639226	2.4668018395763584E-4	0.0	1.566223390207212E-5	9.005784493691467E-5
26-27	0.42666078706405985	2.62342417859708E-4	0.0	1.566223390207212E-5	9.788896188795073E-5
28-29	0.44182574503974115	2.917091064260932E-4	0.0	1.566223390207212E-5	1.1159341655226383E-4
30-31	0.47608492392128615	3.230335742302374E-4	0.0	1.9577792377590146E-5	1.2529787121657695E-4
32-33	0.5397675669671114	3.347802496567915E-4	0.0	1.9577792377590146E-5	1.3704454664313103E-4
34-35	0.6408007223109031	3.700202759364538E-4	0.0	1.9577792377590146E-5	1.4096010511864906E-4
36-37	0.7823520767593555	3.9351362678956194E-4	0.0	2.3493350853108177E-5	1.4096010511864906E-4
38-39	0.9644686170141745	4.0330252297835704E-4	0.0	2.545113009086719E-5	1.4096010511864906E-4
40-41	1.1907095857296062	4.1113363992939305E-4	0.0	2.7408909328626204E-5	1.429178843564081E-4
42-43	1.4662924645742783	4.3462699078250126E-4	0.0	2.7408909328626204E-5	1.6445345597175724E-4
44-45	1.7899309925889832	4.444158869712963E-4	0.0	3.132446780414423E-5	1.8403124834934738E-4
46-47	2.16054251785524	4.6203590011112747E-4	0.0	3.719780551742128E-5	2.0165126148917852E-4
48-49	2.5757855364046893	4.7965591325095864E-4	0.0	3.915558475518029E-5	2.114401576779736E-4
50-51	3.0342915605501375	4.992337056285487E-4	0.0	3.915558475518029E-5	2.2122905386676866E-4
52-53	3.5365382884255974	5.305581734326929E-4	0.0	3.915558475518029E-5	2.4276462548211784E-4
54-55	4.079604713408333	5.579670827613192E-4	0.0	3.915558475518029E-5	2.66257976335226E-4
56-57	4.667030500360212	5.853759920899454E-4	0.0	3.915558475518029E-5	2.72131314048503E-4
58-59	5.289018921975488	6.010382259920176E-4	0.0	3.915558475518029E-5	2.858357687128162E-4
60-61	5.941302019515849	6.245315768451257E-4	0.0	4.11133639929393E-5	2.917091064260932E-4
62-63	6.6259667856487425	6.323626937961618E-4	0.0	4.307114323069832E-5	3.0149800261488827E-4
64-65	7.342568804487199	6.499827069359929E-4	0.0	4.307114323069832E-5	3.0541356109040627E-4
66-67	8.082856036544063	6.63687161600306E-4	0.0	4.307114323069832E-5	3.1324467804144234E-4
68-69	8.845285751779983	6.754338370268601E-4	0.0	4.6986701706216354E-5	3.230335742302374E-4
70-71	9.625445115793049	6.930538501666912E-4	0.0	5.090226018173438E-5	3.2694913270575545E-4
72-73	10.425356514535865	7.126316425442814E-4	0.0	5.090226018173438E-5	3.328224704190325E-4
74-75	11.24308174656305	7.302516556841125E-4	0.0	5.6775597895011425E-5	3.426113666078276E-4
76-77	12.07861493853689	7.361249933973895E-4	0.0	5.873337713277044E-5	3.484847043211046E-4
78-79	12.929111437224925	7.459138895861846E-4	0.0	5.873337713277044E-5	3.621891589854177E-4
80-81	13.790589119657547	7.694072404392928E-4	0.0	6.069115637052946E-5	3.6806249669869476E-4
82-83	14.658670391459133	7.870272535791239E-4	0.0	6.264893560828848E-5	3.7589361364973083E-4
84-85	15.541961650158868	8.00731708243437E-4	0.0	6.264893560828848E-5	3.876402890762849E-4
86-87	16.43571528110519	8.124783836699911E-4	0.0	6.65644940838065E-5	4.05260302216116E-4
88	17.10986285717284	8.183517213832682E-4	0.0	7.048005255932453E-5	4.111336399293931E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	131110	0.0	82.38828	8
GTGGTAT	132445	0.0	81.661995	6
TGGTATC	132665	0.0	81.319885	7
GTATCAA	132750	0.0	81.14394	9
AGTGGTA	133490	0.0	81.12479	5
AAGCAGT	138200	0.0	79.010826	1
GCAGTGG	142485	0.0	76.426674	3
CAGTGGT	142310	0.0	76.38412	4
AGCAGTG	150140	0.0	72.855194	2
CAACGCA	127550	0.0	44.274406	12-13
ATCAACG	129445	0.0	43.71484	10-11
ACGCAGA	131405	0.0	43.040573	14-15
AGAGTAC	132820	0.0	42.387203	18-19
TCAACGC	127315	0.0	42.101326	12-13
AACGCAG	129625	0.0	41.235714	14-15
AGTACTT	134700	0.0	40.94701	20-21
GAGTACT	127230	0.0	40.848953	20-21
TATCAAC	134435	0.0	40.004032	10-11
CGCAGAG	133835	0.0	39.867195	16-17
GCAGAGT	145225	0.0	39.008377	16-17
>>END_MODULE