FastQCFastQC Report
Thu 26 May 2016
SRR1660676_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660676_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24244834
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7308673.0145267235073665No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC467210.19270496964425493Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA286720.11826024463603257No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT286330.11809938562582031No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1022700.079.608898
GTGGTAT1034550.078.855736
AGTGGTA1042150.078.394465
TGGTATC1043050.077.998757
GTATCAA1040350.077.937129
AAGCAGT1071950.076.976341
GCAGTGG1111900.074.104543
CAGTGGT1135600.072.139814
AGCAGTG1190100.069.487982
CAACGCA993450.043.555212-13
ATCAACG1006150.043.12534310-11
ACGCAGA1027150.042.20166814-15
AGAGTAC1035850.041.47456418-19
TCAACGC990750.040.83771512-13
AACGCAG1011200.039.9700114-15
GAGTACT979650.039.550120-21
AGTACTT1052400.039.46813620-21
CGCAGAG1045500.038.65870316-17
TATCAAC1050250.038.5921210-11
GTACTTT1032250.037.51978722-23
Produced by FastQC (version 0.11.5)