FastQCFastQC Report
Thu 26 May 2016
SRR1660676_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660676_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24244834
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6990072.883117285933985No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAACATCTCGTATG296000.12208786416108273TruSeq Adapter, Index 5 (97% over 36bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT291120.12007506423842704No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA274510.1132241202393879No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA991050.078.781928
GTGGTAT1015900.077.132326
TGGTATC1014050.076.995047
AGTGGTA1025800.076.80475
GTATCAA1013300.076.801659
AAGCAGT1042600.076.232871
GCAGTGG1092500.072.610143
CAGTGGT1110250.071.186924
AGCAGTG1156800.068.7779542
CAACGCA966500.043.20128612-13
ATCAACG975150.042.70966710-11
ACGCAGA1001200.041.83464414-15
AGAGTAC1018550.040.94138318-19
TCAACGC957900.040.47213412-13
AACGCAG984650.039.46240614-15
AGTACTT1024650.039.36091220-21
GAGTACT955600.039.23010620-21
CGCAGAG1017200.038.22593716-17
TATCAAC1026250.037.95287710-11
GTACTTT1011150.037.0049722-23
Produced by FastQC (version 0.11.5)