FastQCFastQC Report
Thu 26 May 2016
SRR1660675_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660675_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22862119
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8626843.773421002663839No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA293400.1283345607640307No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT253570.11091272860577796No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1152000.081.823088
GTGGTAT1157050.081.541736
AGTGGTA1163000.081.1648255
TGGTATC1167900.080.543847
GTATCAA1167800.080.498699
AAGCAGT1222250.078.114711
CAGTGGT1245000.076.04814
GCAGTGG1256900.075.739563
AGCAGTG1334350.071.633352
CAACGCA1116700.044.24672312-13
ATCAACG1133100.043.7232110-11
ACGCAGA1143700.043.19614-15
AGAGTAC1156350.042.35085718-19
TCAACGC1114550.042.0639312-13
AACGCAG1131500.041.29884714-15
AGTACTT1157850.041.2272620-21
GAGTACT1103150.040.82034320-21
CGCAGAG1160900.040.163916-17
TATCAAC1172250.039.9842810-11
GTACTTT1150100.039.11011522-23
Produced by FastQC (version 0.11.5)