FastQCFastQC Report
Thu 26 May 2016
SRR1660675_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660675_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22862119
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7411363.2417642476622572No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT263630.11531302063470143No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA231130.10109736547167829No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1011150.080.4866948
GTGGTAT1025300.079.540886
AGTGGTA1035200.079.175065
TGGTATC1031350.078.983187
GTATCAA1028400.078.830559
AAGCAGT1083750.076.165131
GCAGTGG1114950.073.8447343
CAGTGGT1114500.073.735434
AGCAGTG1200650.068.9158252
CAACGCA982350.043.6543212-13
ATCAACG996350.042.99376710-11
ACGCAGA1013800.042.4107514-15
AGAGTAC1025750.041.75762618-19
TCAACGC977950.041.27805712-13
AACGCAG998300.040.43359414-15
GAGTACT973600.040.39517220-21
AGTACTT1039150.040.3092720-21
CGCAGAG1026200.039.38319416-17
TATCAAC1032250.039.2227510-11
GTACTTT1027300.038.19240622-23
Produced by FastQC (version 0.11.5)