FastQCFastQC Report
Thu 26 May 2016
SRR1660674_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660674_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22152069
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7943903.5860758649677376No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT244990.11059463565231763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1058550.081.69638
GTGGTAT1075800.080.5475856
GTATCAA1076050.080.189939
AGTGGTA1086050.079.946295
TGGTATC1083150.079.721717
AAGCAGT1126050.077.9563451
GCAGTGG1160850.075.509613
CAGTGGT1179850.073.8194054
AGCAGTG1209700.072.685052
CAACGCA1037900.043.88627212-13
ATCAACG1052400.043.38205310-11
ACGCAGA1069900.042.67468314-15
AGAGTAC1067450.042.22984318-19
TCAACGC1033700.041.6848712-13
AACGCAG1052600.040.88282414-15
GAGTACT1021700.040.78698320-21
AGTACTT1097450.040.16592820-21
CGCAGAG1078950.039.81688716-17
TATCAAC1087350.039.61743510-11
GTACTTT1067800.038.97852322-23
Produced by FastQC (version 0.11.5)