FastQCFastQC Report
Thu 26 May 2016
SRR1660674_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660674_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22152069
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7374273.3289305843169776No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT249740.11273890488513735No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1006450.080.3260968
GTGGTAT1019450.079.449266
GTATCAA1022650.078.8468869
AGTGGTA1034900.078.780715
TGGTATC1032350.078.283537
AAGCAGT1065700.077.323181
GCAGTGG1098750.074.660183
CAGTGGT1127150.072.55814
AGCAGTG1145450.071.961522
CAACGCA982850.043.6394912-13
ATCAACG992900.043.0652810-11
ACGCAGA1012050.042.41185814-15
AGAGTAC1026300.041.57426518-19
TCAACGC975450.041.25399812-13
AACGCAG999150.040.3260514-15
GAGTACT980200.040.07137720-21
AGTACTT1056650.039.5757520-21
CGCAGAG1024450.039.30788416-17
TATCAAC1034450.038.9737410-11
GTACTTT1026250.038.1660822-23
Produced by FastQC (version 0.11.5)