FastQCFastQC Report
Thu 26 May 2016
SRR1660673_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660673_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56531656
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16497092.9182039174652874No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1417850.2508063800572196No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT942270.16668006329055707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2198400.081.91228
GTGGTAT2242250.080.322326
TGGTATC2241400.080.194487
AGTGGTA2251400.080.0952455
GTATCAA2250500.079.8627249
AAGCAGT2420700.075.468661
GCAGTGG2453450.074.2708053
CAGTGGT2452600.073.780824
AGCAGTG2630450.069.5907062
CAACGCA2137100.044.27400612-13
ATCAACG2176300.043.49372510-11
ACGCAGA2204400.042.84852214-15
TCAACGC2126500.042.3592312-13
AGAGTAC2211200.042.1334918-19
AACGCAG2168500.041.4127714-15
GAGTACT2134950.040.4535620-21
CGCAGAG2240250.039.96141416-17
TATCAAC2262250.039.70921710-11
AGTACTT2295200.039.7039720-21
GTACTTT2275100.037.93867522-23
Produced by FastQC (version 0.11.5)