##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660673_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 56531656 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.373863008718516 34.0 31.0 34.0 31.0 34.0 2 32.52716730958669 34.0 31.0 34.0 31.0 34.0 3 32.5497687879513 34.0 31.0 34.0 31.0 34.0 4 35.90861918143703 37.0 37.0 37.0 35.0 37.0 5 35.90647015541169 37.0 37.0 37.0 35.0 37.0 6 35.92404100102782 37.0 37.0 37.0 35.0 37.0 7 35.8970270037729 37.0 37.0 37.0 35.0 37.0 8 35.90410933654588 37.0 37.0 37.0 35.0 37.0 9 37.635418428216575 39.0 38.0 39.0 35.0 39.0 10-11 37.58059713481593 39.0 38.0 39.0 35.0 39.0 12-13 37.61521268720662 39.0 38.0 39.0 35.0 39.0 14-15 39.09468700156245 41.0 39.0 41.0 36.0 41.0 16-17 39.049470512592094 41.0 39.0 41.0 36.0 41.0 18-19 39.00586996779291 41.0 39.0 41.0 36.0 41.0 20-21 38.9470719378891 41.0 39.0 41.0 35.0 41.0 22-23 38.832250182092665 40.5 39.0 41.0 35.0 41.0 24-25 38.80864923716369 40.0 39.0 41.0 35.0 41.0 26-27 38.7579328916174 40.0 39.0 41.0 35.0 41.0 28-29 38.67114601242179 40.0 38.5 41.0 35.0 41.0 30-31 38.57381657101996 40.0 38.0 41.0 34.0 41.0 32-33 38.46854894892872 40.0 38.0 41.0 34.0 41.0 34-35 38.36948674385197 40.0 38.0 41.0 34.0 41.0 36-37 38.23102671890595 40.0 38.0 41.0 34.0 41.0 38-39 37.94433847825013 40.0 37.5 41.0 33.0 41.0 40-41 37.80911540075883 40.0 37.0 41.0 33.0 41.0 42-43 37.661324356038676 40.0 37.0 41.0 33.0 41.0 44-45 37.5262960543735 40.0 37.0 41.0 32.5 41.0 46-47 37.35625487426019 40.0 36.0 41.0 32.0 41.0 48-49 37.38767949766057 40.0 36.0 41.0 32.0 41.0 50-51 36.43415483883932 38.5 35.5 40.0 31.5 40.5 52-53 36.659609661885725 39.0 35.5 39.5 31.5 40.5 54-55 37.187368047382165 40.0 36.0 41.0 31.5 41.0 56-57 37.00011028334284 40.0 36.0 41.0 31.0 41.0 58-59 36.801270486397925 39.5 35.5 41.0 31.0 41.0 60-61 36.58474446600326 39.0 35.0 41.0 30.5 41.0 62-63 36.32301684210347 39.0 35.0 41.0 30.0 41.0 64-65 36.03297786818769 38.5 35.0 41.0 30.0 41.0 66-67 35.71230880800661 37.0 35.0 40.0 29.0 41.0 68-69 35.3945400785712 37.0 35.0 40.0 29.0 41.0 70-71 35.049034438333095 36.0 35.0 39.0 29.0 41.0 72-73 34.689335263767966 36.0 34.5 39.0 29.0 41.0 74-75 34.33159850296973 35.0 34.0 38.5 28.0 40.5 76-77 33.97481067952441 35.0 34.0 37.0 28.0 39.5 78-79 33.632337959461154 35.0 34.0 37.0 27.0 39.0 80-81 33.313424322825426 35.0 34.0 36.0 27.0 39.0 82-83 33.04139846000619 35.0 34.0 36.0 26.0 37.0 84-85 32.79808548329099 35.0 33.5 35.5 26.0 37.0 86-87 32.591891028276265 35.0 33.0 35.0 26.0 37.0 88-89 32.40117471527811 35.0 33.0 35.0 25.0 36.0 90-91 32.26660582877671 35.0 33.0 35.0 25.0 36.0 92-93 32.13697152264565 35.0 33.0 35.0 25.0 36.0 94-95 32.02987885230179 35.0 33.0 35.0 25.0 35.5 96-97 31.92896804579721 35.0 33.0 35.0 24.0 35.0 98-99 31.68582082576884 35.0 33.0 35.0 23.5 35.0 100 31.61924842605 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4902844256408514 1101 2 0.47943126573228056 1101 3 0.4732730601591655 1101 4 0.40574126606404803 1101 5 0.3944753458180088 1101 6 0.38674479699685094 1101 7 0.39022469165642804 1101 8 0.39624353739686313 1101 9 0.5118581561902218 1101 10-11 0.4803345563080512 1101 12-13 0.47513063540095146 1101 14-15 0.6234607699698387 1101 16-17 0.6320368615720469 1101 18-19 0.5986844085196807 1101 20-21 0.6235530584166327 1101 22-23 0.6396694375644714 1101 24-25 0.6497904651762312 1101 26-27 0.6109920427476041 1101 28-29 0.6107589421493387 1101 30-31 0.6224812021232751 1101 32-33 0.6434532701691822 1101 34-35 0.6274507962185965 1101 36-37 0.6337012599579808 1101 38-39 0.6641443557968998 1101 40-41 0.674146920821812 1101 42-43 0.6628238025948434 1101 44-45 0.6636974759885064 1101 46-47 0.6488123616308883 1101 48-49 0.6496088415454224 1101 50-51 0.5951033401308194 1101 52-53 0.6523619103105034 1101 54-55 0.7219775505603323 1101 56-57 0.6954889181475465 1101 58-59 0.6599181535260854 1101 60-61 0.6495587865257093 1101 62-63 0.6252053130251269 1101 64-65 0.6380544193006585 1101 66-67 0.6083985794518227 1101 68-69 0.5799112593445486 1101 70-71 0.5639769273228552 1101 72-73 0.5164367368089486 1101 74-75 0.5197158838642579 1101 76-77 0.5225156355290395 1101 78-79 0.5182306961244407 1101 80-81 0.5293554332507426 1101 82-83 0.4957577569524432 1101 84-85 0.5097220211299813 1101 86-87 0.5126240718670019 1101 88-89 0.4927425437625743 1101 90-91 0.4952955579144387 1101 92-93 0.4940576883759462 1101 94-95 0.4726219423928839 1101 96-97 0.45959278527518777 1101 98-99 0.28825268383909375 1101 100 0.3396893974724762 1102 1 0.4737607619099222 1102 2 0.4448177352823208 1102 3 0.46205488296025266 1102 4 0.37842491186780336 1102 5 0.39756469448990117 1102 6 0.3568476624654835 1102 7 0.36276459261883787 1102 8 0.3971549158741894 1102 9 0.447969544237111 1102 10-11 0.4858091365431605 1102 12-13 0.45876370094626395 1102 14-15 0.5509759761698945 1102 16-17 0.5885688203171426 1102 18-19 0.5855077844129752 1102 20-21 0.5507320280057897 1102 22-23 0.5780103002963344 1102 24-25 0.5998398485452086 1102 26-27 0.5764921068011901 1102 28-29 0.5761951153754339 1102 30-31 0.5720293696113004 1102 32-33 0.6095512126746172 1102 34-35 0.5815491914629476 1102 36-37 0.6156527052870402 1102 38-39 0.6334404939948612 1102 40-41 0.6171669985507364 1102 42-43 0.6360150263396491 1102 44-45 0.5976307261230289 1102 46-47 0.6228886864137735 1102 48-49 0.6060052874452779 1102 50-51 0.5991564802635523 1102 52-53 0.6350063076470889 1102 54-55 0.6813423441795763 1102 56-57 0.6787679030044984 1102 58-59 0.6539454540595173 1102 60-61 0.6512468663548745 1102 62-63 0.6406135644994393 1102 64-65 0.6236896810569732 1102 66-67 0.5886505088415603 1102 68-69 0.5967417829892767 1102 70-71 0.5735087749047096 1102 72-73 0.5356434832500625 1102 74-75 0.5349337954691293 1102 76-77 0.5395307888132166 1102 78-79 0.49975887678501607 1102 80-81 0.4936641686198371 1102 82-83 0.47275354610906817 1102 84-85 0.4884338684690377 1102 86-87 0.5018969953142971 1102 88-89 0.49738716272661776 1102 90-91 0.5113349460438386 1102 92-93 0.5127400196269463 1102 94-95 0.4993119968838471 1102 96-97 0.46779067571089783 1102 98-99 0.3349669277219327 1102 100 0.3654984768351994 1103 1 0.3902153384406546 1103 2 0.3840320190309825 1103 3 0.3669373242683278 1103 4 0.33425157803522865 1103 5 0.3559254991710503 1103 6 0.2965066727732406 1103 7 0.3318534950564569 1103 8 0.34003170233204116 1103 9 0.42952868625101814 1103 10-11 0.42785573422711565 1103 12-13 0.39579973516408984 1103 14-15 0.4899580942234394 1103 16-17 0.5228574576383167 1103 18-19 0.5051718028258492 1103 20-21 0.5091604205827807 1103 22-23 0.5224407567616609 1103 24-25 0.5080890338094903 1103 26-27 0.5048503979660026 1103 28-29 0.5228678480866904 1103 30-31 0.5245910524317026 1103 32-33 0.5092499126824706 1103 34-35 0.5046440005510888 1103 36-37 0.5036226369400296 1103 38-39 0.5289914493376315 1103 40-41 0.5577496616001127 1103 42-43 0.5614378057708649 1103 44-45 0.5511841661116677 1103 46-47 0.5476361851000888 1103 48-49 0.5030041621112034 1103 50-51 0.4467798878245688 1103 52-53 0.5339189226068157 1103 54-55 0.5718494104152327 1103 56-57 0.5739851225568486 1103 58-59 0.5706914094290596 1103 60-61 0.5611972510529384 1103 62-63 0.5334275469126695 1103 64-65 0.518001387408944 1103 66-67 0.5093476168858686 1103 68-69 0.47836007608698594 1103 70-71 0.49037090849782317 1103 72-73 0.4451884384495344 1103 74-75 0.47507166599397976 1103 76-77 0.4692377067993476 1103 78-79 0.46502095852748937 1103 80-81 0.438400461069989 1103 82-83 0.43900465614654394 1103 84-85 0.4688920988617298 1103 86-87 0.43851320004168315 1103 88-89 0.4314322754386737 1103 90-91 0.44521374775658984 1103 92-93 0.4430700480362546 1103 94-95 0.4324938959548277 1103 96-97 0.4467111606753029 1103 98-99 0.2916963465435849 1103 100 0.32657823659539886 1104 1 0.35246311300793565 1104 2 0.351937375395849 1104 3 0.33673370398473423 1104 4 0.2977291290851696 1104 5 0.3136435049346389 1104 6 0.2922848324788845 1104 7 0.3041294540667039 1104 8 0.3318596835269929 1104 9 0.3826934419970627 1104 10-11 0.38953925950600876 1104 12-13 0.35643581746951725 1104 14-15 0.4521127363142483 1104 16-17 0.44300530807708327 1104 18-19 0.4453482297252762 1104 20-21 0.46670847204541843 1104 22-23 0.4600956646172065 1104 24-25 0.4648660498732653 1104 26-27 0.46019760721215874 1104 28-29 0.4636066832930439 1104 30-31 0.46544894959010463 1104 32-33 0.455521654089452 1104 34-35 0.46491507891938255 1104 36-37 0.4380557428546936 1104 38-39 0.48460890983287896 1104 40-41 0.45300587322097385 1104 42-43 0.49722517997150106 1104 44-45 0.4810522874920693 1104 46-47 0.46862999609889044 1104 48-49 0.45269635462290836 1104 50-51 0.36771781424781835 1104 52-53 0.4369874221241119 1104 54-55 0.5055858555813231 1104 56-57 0.4998059464770108 1104 58-59 0.4912956303399838 1104 60-61 0.4696599600033622 1104 62-63 0.4932793748154509 1104 64-65 0.4537719111045817 1104 66-67 0.43837576046096416 1104 68-69 0.44158336223649997 1104 70-71 0.4397799165612071 1104 72-73 0.4099753993054662 1104 74-75 0.40407286998976133 1104 76-77 0.38925656325201885 1104 78-79 0.3787309618492003 1104 80-81 0.3937895913605658 1104 82-83 0.39378744188100256 1104 84-85 0.3866810230243303 1104 86-87 0.3852285725205604 1104 88-89 0.3857168357787728 1104 90-91 0.40806457621264514 1104 92-93 0.4076394750954222 1104 94-95 0.4088725167234841 1104 96-97 0.387375519872986 1104 98-99 0.23084994467872022 1104 100 0.2959059081752926 1105 1 0.32159012875657567 1105 2 0.3088207932416651 1105 3 0.3086308541874132 1105 4 0.29961546062690303 1105 5 0.30434406378104484 1105 6 0.2692938066794426 1105 7 0.2635747126649548 1105 8 0.27295714441205376 1105 9 0.34251532173793464 1105 10-11 0.3132227679566526 1105 12-13 0.3367890544427894 1105 14-15 0.42741755920137336 1105 16-17 0.42224986279311594 1105 18-19 0.4347330342413187 1105 20-21 0.4168522024340646 1105 22-23 0.4341266473402854 1105 24-25 0.4242754116629399 1105 26-27 0.4203153053896713 1105 28-29 0.4354003990467987 1105 30-31 0.44142392162697064 1105 32-33 0.42477231973088436 1105 34-35 0.42868107213731577 1105 36-37 0.4388197090280457 1105 38-39 0.4526947793788665 1105 40-41 0.4611658880318217 1105 42-43 0.4469047311815686 1105 44-45 0.4709273553234752 1105 46-47 0.4691037306599952 1105 48-49 0.4307700167960533 1105 50-51 0.3675631976687228 1105 52-53 0.43677649685676556 1105 54-55 0.4849790568932377 1105 56-57 0.4381664731347854 1105 58-59 0.46202856166321027 1105 60-61 0.41185790378462883 1105 62-63 0.4237261000053607 1105 64-65 0.44053043976377637 1105 66-67 0.4180641072533646 1105 68-69 0.4145975500564276 1105 70-71 0.39045464383454487 1105 72-73 0.3970525870678827 1105 74-75 0.395438969772556 1105 76-77 0.38811620864606766 1105 78-79 0.38700372783490167 1105 80-81 0.37671438859477746 1105 82-83 0.3809520237516111 1105 84-85 0.38390322797059895 1105 86-87 0.3686552003833867 1105 88-89 0.375827533008227 1105 90-91 0.3677598206726742 1105 92-93 0.37034674735070183 1105 94-95 0.3705872938379642 1105 96-97 0.3792852881360993 1105 98-99 0.2570446260526644 1105 100 0.24848083286014244 1106 1 0.35193679344995843 1106 2 0.34294946190375697 1106 3 0.3570476049664677 1106 4 0.3171579731586647 1106 5 0.30470524423922996 1106 6 0.3037486562051157 1106 7 0.30944203958517136 1106 8 0.3305973380083316 1106 9 0.38872152491536127 1106 10-11 0.38090804632302167 1106 12-13 0.37285015714958547 1106 14-15 0.42863898804182554 1106 16-17 0.4691833667546703 1106 18-19 0.4571536811332919 1106 20-21 0.47283964875413886 1106 22-23 0.48796319595906823 1106 24-25 0.45687326650377 1106 26-27 0.44152677836881793 1106 28-29 0.4657405116006572 1106 30-31 0.4866721727525558 1106 32-33 0.4832273795832336 1106 34-35 0.4873720688493819 1106 36-37 0.48497779669686736 1106 38-39 0.4751909936151435 1106 40-41 0.4897149187290495 1106 42-43 0.5049219395732081 1106 44-45 0.49746546342792186 1106 46-47 0.5060720321004339 1106 48-49 0.4825756564695354 1106 50-51 0.4527458050658808 1106 52-53 0.44632623023854023 1106 54-55 0.4600625734022472 1106 56-57 0.47283296491151816 1106 58-59 0.43861271093072673 1106 60-61 0.4354391253992915 1106 62-63 0.422944724124541 1106 64-65 0.4005228448002214 1106 66-67 0.3885101226267267 1106 68-69 0.3961252403920099 1106 70-71 0.37257730234637165 1106 72-73 0.39081958261471783 1106 74-75 0.3883449714691949 1106 76-77 0.36191889157932877 1106 78-79 0.3692705708142867 1106 80-81 0.370678707092317 1106 82-83 0.3681118015799001 1106 84-85 0.3977415851594017 1106 86-87 0.39459778266500223 1106 88-89 0.42039125342939343 1106 90-91 0.39791437487337333 1106 92-93 0.37787778266819316 1106 94-95 0.41542189752510694 1106 96-97 0.42177598275164385 1106 98-99 0.3012731717931594 1106 100 0.33930845085624384 1107 1 0.3000043236811152 1107 2 0.26623943684118956 1107 3 0.2508990200623771 1107 4 0.26447879629386506 1107 5 0.27721950994887834 1107 6 0.2321681476627191 1107 7 0.212328288211026 1107 8 0.25444419580900757 1107 9 0.3200489750491897 1107 10-11 0.33481731747163934 1107 12-13 0.2954308514542632 1107 14-15 0.34714157002332513 1107 16-17 0.3750208288157708 1107 18-19 0.3941552638286012 1107 20-21 0.3689540662168085 1107 22-23 0.36992791699510263 1107 24-25 0.386723700253917 1107 26-27 0.3596735594458167 1107 28-29 0.3702805835911178 1107 30-31 0.39565232547274576 1107 32-33 0.39675110331685204 1107 34-35 0.3895472517484464 1107 36-37 0.3989039042354321 1107 38-39 0.41045094924526637 1107 40-41 0.42403963721302773 1107 42-43 0.41521470232554236 1107 44-45 0.45247382878172715 1107 46-47 0.43449517386014946 1107 48-49 0.3764093944895208 1107 50-51 0.28343952678658724 1107 52-53 0.3583471191371288 1107 54-55 0.4445001601992047 1107 56-57 0.44739137040159704 1107 58-59 0.4501581341294312 1107 60-61 0.43544328365846496 1107 62-63 0.42266917532084136 1107 64-65 0.3979404742372594 1107 66-67 0.40067481621262857 1107 68-69 0.4122790464298305 1107 70-71 0.38261236808769183 1107 72-73 0.40053301864299584 1107 74-75 0.39607239513470915 1107 76-77 0.3703323730356587 1107 78-79 0.3681246205538855 1107 80-81 0.3483481846194536 1107 82-83 0.33271174738872134 1107 84-85 0.37876117752318805 1107 86-87 0.38115878420767757 1107 88-89 0.3898077660597892 1107 90-91 0.3820155585888827 1107 92-93 0.3663494898097497 1107 94-95 0.35612515503152764 1107 96-97 0.3691081023914897 1107 98-99 0.22817599766563745 1107 100 0.27468676133210934 1108 1 0.1354901774494408 1108 2 0.1330821558998707 1108 3 0.13823246640496478 1108 4 0.15001608730570837 1108 5 0.13171845604286148 1108 6 0.1301305292622601 1108 7 0.10738092087235174 1108 8 0.11285513501557887 1108 9 0.15673370090689076 1108 10-11 0.17648984685544633 1108 12-13 0.15235226428622184 1108 14-15 0.14410288183465525 1108 16-17 0.19387237569259952 1108 18-19 0.16942742233077723 1108 20-21 0.190229541779523 1108 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sequence quality scores pass #Quality Count 2 1524.0 3 555.0 4 484.0 5 503.0 6 1489.0 7 11852.0 8 28596.0 9 39446.0 10 44407.0 11 44358.0 12 45826.0 13 49428.0 14 59293.0 15 77274.0 16 99065.0 17 121979.0 18 134750.0 19 149212.0 20 170821.0 21 203051.0 22 257654.0 23 358751.0 24 495879.0 25 626469.0 26 674874.0 27 640394.0 28 584006.0 29 610955.0 30 708344.0 31 865636.0 32 1089943.0 33 1424029.0 34 2022894.0 35 3336718.0 36 6179778.0 37 1.3170651E7 38 1.7084676E7 39 5105408.0 40 10684.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.7109828833955 31.31252430367174 27.860871076541795 23.115621736390967 2 22.00059692642358 30.618405368260138 27.12383724297015 20.257160462346132 3 23.619333506342443 26.133569426841387 27.036336584201425 23.21076048261474 4 20.278139785749897 27.526235492424057 26.157730291033637 26.037894430792406 5 22.136832522977404 29.767029021433235 26.78658436724107 21.309554088348296 6 25.793252267732463 26.859136981698455 26.463629710331453 20.88398104023762 7 21.963342197771986 26.419763167570736 29.884544969434607 21.73234966522267 8 24.976506480930198 26.829624438703505 26.485184965018497 21.708684115347804 9 24.548701097448273 26.05710211999142 27.48070645842626 21.913490324134045 10-11 21.57542611787494 28.57516778754452 28.27420998898568 21.575196105594852 12-13 21.522714780468274 26.900337207640217 28.1742816848141 23.402666327077405 14-15 21.52493365223944 30.279724373467182 26.57465758625421 21.620684388039166 16-17 23.35345004744651 26.709506333459597 26.54656025030081 23.39048336879308 18-19 21.630350593209936 28.434422840600842 26.584796884336175 23.35042968185304 20-21 23.313427834176004 28.529289056793928 26.544353799944513 21.61292930908556 22-23 23.3865922553831 26.81718507448947 28.162363591091356 21.633859079036075 24-25 21.717900904084065 28.385824955764623 26.3976059120414 23.498668228109917 26-27 21.71755164215267 26.788895402954417 29.826856727981692 21.66669622691122 28-29 21.804498142025235 26.80147785214375 29.771651754229566 21.622372251601448 30-31 21.784138671555855 26.70150570694267 29.808336360415154 21.706019261086322 32-33 21.846835138264858 26.795597335151793 29.689536456885268 21.66803106969808 34-35 21.86017714473289 26.95263311573177 29.558607400705817 21.628582338829528 36-37 21.79059132766505 26.9244010268764 29.61983614002344 21.665171505435108 38-39 21.854938183635774 26.871866802520938 29.608208302661826 21.66498671118146 40-41 21.946818838562823 26.940845412732667 29.512774565772894 21.599561182931616 42-43 21.983448886992274 26.958985894987137 29.483873692009936 21.573691526010652 44-45 22.100624899147032 27.024540181238095 29.373147490211952 21.50168742940292 46-47 22.061303753047028 27.12884258303832 29.31752778681172 21.49232587710293 48-49 22.20616659347712 27.12137587345203 29.15793015218209 21.514527380888758 50-51 22.28600372195134 27.161228293482836 29.094792275977845 21.457975708587984 52-53 22.37778070217777 27.308243597345083 28.952234880101095 21.361740820376056 54-55 22.648542771385745 27.410948260831702 28.470422271851458 21.470086695931094 56-57 22.98012100152236 27.773939507024476 27.872911154221246 21.373028337231915 58-59 23.181246302908303 28.01117975315669 27.532258279262667 21.27531566467234 60-61 23.36179268329008 28.154694866496648 27.28568925090558 21.197823199307688 62-63 23.503232217781356 28.232528543028334 27.175617698433317 21.088621540756993 64-65 23.681319550987205 28.29143128979945 27.105756415761185 20.92149274345216 66-67 23.841231258102646 28.180971221999755 27.163100701180447 20.814696818717152 68-69 24.006255479633467 28.080097090105678 27.246212138604065 20.667435291656787 70-71 24.185806623361298 27.997611365043934 27.286384362253568 20.5301976493412 72-73 24.35464156078309 27.955312053405112 27.233042904210414 20.45700348160138 74-75 24.583179433476754 27.975560820751415 27.13123547477935 20.310024270992482 76-77 24.83173587488302 28.031256557713025 26.926093112030415 20.210914455373537 78-79 25.08534999039216 28.051191927000158 26.705453652436688 20.158004430170994 80-81 25.320221507684476 28.087866083243835 26.510873530210375 20.081038878861314 82-83 25.50284889400512 28.136158118049963 26.332640124931817 20.028352863013104 84-85 25.65534717968817 28.07568485420245 26.253971047672813 20.01499691843657 86-87 25.829160939099268 28.01283464683973 26.188055562240663 19.96994885182034 88-89 25.980034742199038 27.990476442513913 26.12186246011615 19.907626355170898 90-91 26.11091859553471 27.91741675186823 26.08140389900847 19.89026075358859 92-93 26.219137231186334 27.92370826322526 26.050831854729 19.806322650859403 94-95 26.294532364749056 27.963483496738327 26.00221659286429 19.739767545648327 96-97 26.343482395098395 27.989146853236935 25.93592712974638 19.73144362191829 98-99 26.400446767237856 28.08506368569627 25.867798583945252 19.646690963120623 100 26.401974004796084 28.202817750368247 25.776104093913155 19.61910415092251 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 197611.0 1 116835.0 2 27580.0 3 17342.0 4 15367.0 5 14906.5 6 14937.0 7 15092.5 8 14840.5 9 15091.0 10 16304.5 11 23485.5 12 195646.5 13 224964.5 14 84585.5 15 85129.5 16 94476.0 17 103984.5 18 113152.5 19 123611.0 20 138886.5 21 164680.5 22 202641.0 23 245042.5 24 289298.0 25 340990.5 26 397317.5 27 463268.5 28 552035.0 29 642820.0 30 720612.0 31 808208.5 32 901645.0 33 979151.0 34 1053289.0 35 1152807.5 36 1260959.5 37 1342459.0 38 1428409.5 39 1516150.5 40 1576521.5 41 1651783.5 42 1748221.5 43 1796838.5 44 1819870.5 45 1878099.5 46 1950879.5 47 2013661.5 48 2062269.5 49 2095561.5 50 2108345.5 51 2105417.5 52 2082878.0 53 2025620.0 54 1938198.0 55 1828109.0 56 1697482.0 57 1545365.5 58 1370319.0 59 1172094.5 60 961256.5 61 767632.0 62 599219.5 63 450712.0 64 330532.0 65 239228.0 66 171226.5 67 125267.5 68 92325.5 69 68941.0 70 53010.0 71 41907.0 72 34205.5 73 26898.0 74 21518.5 75 18731.0 76 14312.0 77 8319.5 78 4928.0 79 3575.5 80 2574.5 81 1969.0 82 1533.5 83 1162.0 84 930.5 85 811.5 86 731.5 87 634.5 88 549.0 89 464.0 90 394.5 91 372.0 92 313.5 93 249.5 94 204.5 95 183.5 96 165.0 97 126.0 98 90.0 99 59.5 100 34.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13569211558210856 2 0.05108642138486091 3 0.018658572464249058 4 0.07557181767326965 5 0.07915741934041345 6 0.08086796537501041 7 0.04817124055237299 8 0.045367501705593054 9 0.03331407804505143 10-11 0.022880101018091528 12-13 0.01498364031649807 14-15 0.011964977640138475 16-17 0.01695598657148837 18-19 0.016371358376623533 20-21 0.02288275439870362 22-23 0.019487311675426597 24-25 0.016962177792916592 26-27 0.015333886557294552 28-29 0.017270854404123594 30-31 0.015163185737916468 32-33 0.012268347490121286 34-35 0.009836966389238623 36-37 0.00761962465773159 38-39 0.016495182805187948 40-41 0.025216844877142815 42-43 0.025738676397521417 44-45 0.024297890725154064 46-47 0.026306499848509655 48-49 0.022788117156872248 50-51 0.02284737599054236 52-53 0.01952888130501608 54-55 0.02457738014962802 56-57 0.03094018685743082 58-59 0.023727413893553727 60-61 0.023361247369084674 62-63 0.03057844263398192 64-65 0.031425755509444125 66-67 0.023819397754773007 68-69 0.02449512535065309 70-71 0.035168791092905535 72-73 0.03972729898448402 74-75 0.040770077565037185 76-77 0.04204723809965871 78-79 0.03629647785304573 80-81 0.0319130930818655 82-83 0.03458239397763264 84-85 0.03781951832438802 86-87 0.037741685826433245 88-89 0.036865185764238 90-91 0.0398360875895799 92-93 0.03343967139402391 94-95 0.033250396910361156 96-97 0.033307886823623206 98-99 0.31394622510262216 100 0.14997261003640155 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5.6531656E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.89369610961025 #Duplication Level Percentage of deduplicated Percentage of total 1 74.63855166621546 29.77607698233443 2 11.205944293892516 8.940930725635381 3 4.365564080220677 5.224754602900616 4 2.4104159728208256 3.846416092698583 5 1.547118773688546 3.086014310150186 6 1.0652759420562603 2.5498676823162736 7 0.7464778727494703 2.0845832985610975 8 0.5964012757804376 1.9034121004294908 9 0.45691533803431494 1.6405237479024721 >10 2.6039353235604894 18.83475770940492 >50 0.21024781076534166 5.8342292661924535 >100 0.1490280729866335 11.482804261211168 >500 0.003656369946213558 0.8876823395944129 >1k 4.1424950654533405E-4 0.330512945038776 >5k 3.089205536906635E-5 0.09097915170410908 >10k+ 2.2065720917676145E-5 3.486454783925626 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1649709 2.9182039174652874 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 141785 0.2508063800572196 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 94227 0.16668006329055707 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.11251395147525839 1.7689204080630506E-6 0.0 0.0 8.844602040315254E-6 2 0.11253164067933902 1.0613522448378303E-5 0.0 0.0 1.5920283672567454E-5 3 0.11253517852015515 1.9458124488693556E-5 0.0 1.7689204080630506E-6 1.5920283672567454E-5 4 0.11253517852015515 3.360948775319796E-5 0.0 1.7689204080630506E-6 1.9458124488693556E-5 5 0.11254048528137933 4.4223010201576264E-5 0.0 3.5378408161261013E-6 2.6533806120945757E-5 6 0.11260416641606961 5.483653264995457E-5 1.7689204080630506E-6 3.5378408161261013E-6 3.007164693707186E-5 7 0.11261124209770186 6.721897550639592E-5 1.7689204080630506E-6 5.3067612241891515E-6 3.714732856932406E-5 8 0.11261124209770186 7.252573673058507E-5 1.7689204080630506E-6 7.0756816322522025E-6 4.245408979351321E-5 9 0.11262185562015024 7.429465713864812E-5 1.7689204080630506E-6 7.0756816322522025E-6 4.599193060963932E-5 10-11 0.11263777590382282 8.490817958702642E-5 1.7689204080630506E-6 7.0756816322522025E-6 5.7489913262049147E-5 12-13 0.11266165632933166 9.640616223943627E-5 1.7689204080630506E-6 7.0756816322522025E-6 6.456559489430134E-5 14-15 0.11269261243647277 1.0967306529990913E-4 1.7689204080630506E-6 7.0756816322522025E-6 7.341019693461661E-5 16-17 0.11271472394157356 1.2824672958457116E-4 1.7689204080630506E-6 1.0613522448378303E-5 8.7561560199121E-5 18-19 0.11276425371299931 1.441670132571386E-4 1.7689204080630506E-6 1.3266903060472879E-5 9.286832142331016E-5 20-21 0.11280140104156863 1.6185621733776914E-4 1.7689204080630506E-6 1.5920283672567454E-5 1.0613522448378303E-4 22-23 0.11303047623441281 1.8219880203049422E-4 1.7689204080630506E-6 1.5920283672567454E-5 1.1497982652409829E-4 24-25 0.11418735018128603 1.9546570509096708E-4 1.7689204080630506E-6 1.5920283672567454E-5 1.2559334897247658E-4 26-27 0.11879361892388222 2.140393693756291E-4 1.7689204080630506E-6 1.7689204080630507E-5 1.3001564999263421E-4 28-29 0.13316432831898645 2.2465289182400743E-4 1.7689204080630506E-6 1.7689204080630507E-5 1.406291724410125E-4 30-31 0.16580798552938197 2.485333173328586E-4 1.7689204080630506E-6 1.8573664284662034E-5 1.5301161529745387E-4 32-33 0.2277582316003621 2.688759020255837E-4 1.7689204080630506E-6 2.1227044896756606E-5 1.715852795821159E-4 34-35 0.3290103512976871 2.865651061062142E-4 1.7689204080630506E-6 2.1227044896756606E-5 1.8042988162243116E-4 36-37 0.47876980642491707 3.0690769079893924E-4 1.7689204080630506E-6 2.1227044896756606E-5 1.9546570509096708E-4 38-39 0.685059535492822 3.14867832635223E-4 1.7689204080630506E-6 2.2111505100788132E-5 1.990035459070932E-4 40-41 0.9479856737258855 3.2459689487956977E-4 1.7689204080630506E-6 2.7418266324977284E-5 2.0784814794740845E-4 42-43 1.2614755173632273 3.360948775319796E-4 1.7689204080630506E-6 2.830272652900881E-5 2.1934613059981827E-4 44-45 1.6289041665434318 3.467083999803579E-4 1.7689204080630506E-6 3.007164693707186E-5 2.3615087447641724E-4 46-47 2.056110827533515 3.7147328569324064E-4 1.7689204080630506E-6 3.184056734513491E-5 2.449954765167325E-4 48-49 2.546303437493499 3.8739356936580806E-4 1.7689204080630506E-6 3.184056734513491E-5 2.5384007855704777E-4 50-51 3.0889949517841826 4.02429392834344E-4 1.7689204080630506E-6 3.184056734513491E-5 2.715292826376783E-4 52-53 3.6773759112947264 4.218875173230375E-4 3.5378408161261013E-6 3.360948775319796E-5 2.8214280508605656E-4 54-55 4.308134012561033 4.3426996017947893E-4 3.5378408161261013E-6 3.360948775319796E-5 2.909874071263718E-4 56-57 4.984277304737013 4.4576794283188875E-4 3.5378408161261013E-6 3.891624897738711E-5 2.9806308875862404E-4 58-59 5.707501298033796 4.5991930609639317E-4 3.5378408161261013E-6 3.891624897738711E-5 3.077921510029708E-4 60-61 6.470336726028334 4.873375724213704E-4 3.5378408161261013E-6 3.891624897738711E-5 3.2017459385941217E-4 62-63 7.263200462409946 5.067956969100639E-4 3.5378408161261013E-6 3.891624897738711E-5 3.237124346755383E-4 64-65 8.082841231468613 5.244849009906946E-4 3.5378408161261013E-6 4.0685169385450166E-5 3.290191958997274E-4 66-67 8.92929971837372 5.359828836431043E-4 3.5378408161261013E-6 4.0685169385450166E-5 3.387482581440742E-4 68-69 9.803054416095648 5.430585652753565E-4 3.5378408161261013E-6 4.1569629589481685E-5 3.475928601843894E-4 70-71 10.701450175101893 5.598633091519555E-4 3.5378408161261013E-6 4.4223010201576264E-5 3.5555300202067317E-4 72-73 11.61482780550423 5.740146724164598E-4 3.5378408161261013E-6 4.599193060963932E-5 3.626286836529254E-4 74-75 12.543210303267958 5.917038764970905E-4 3.5378408161261013E-6 4.599193060963932E-5 3.7147328569324064E-4 76-77 13.486067876730871 6.067396999656264E-4 3.5378408161261013E-6 4.8645311221733896E-5 3.8031788773355585E-4 78-79 14.444435344331678 6.226599836381937E-4 3.5378408161261013E-6 5.129869183382847E-5 3.891624897738711E-4 80-81 15.414432579155296 6.394647275147927E-4 3.5378408161261013E-6 5.3067612241891515E-5 4.02429392834344E-4 82-83 16.3970846705782 6.527316305752657E-4 3.5378408161261013E-6 5.395207244592304E-5 4.1038953467062775E-4 84-85 17.385977513200743 6.757275958800854E-4 3.5378408161261013E-6 5.483653264995457E-5 4.218875173230375E-4 86-87 18.38293397950345 6.969546407768419E-4 3.5378408161261013E-6 5.483653264995457E-5 4.271942785472267E-4 88 19.13120146347738 7.128749244494093E-4 3.5378408161261013E-6 5.660545305801762E-5 4.298476591593213E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 219840 0.0 81.9122 8 GTGGTAT 224225 0.0 80.32232 6 TGGTATC 224140 0.0 80.19448 7 AGTGGTA 225140 0.0 80.095245 5 GTATCAA 225050 0.0 79.862724 9 AAGCAGT 242070 0.0 75.46866 1 GCAGTGG 245345 0.0 74.270805 3 CAGTGGT 245260 0.0 73.78082 4 AGCAGTG 263045 0.0 69.590706 2 CAACGCA 213710 0.0 44.274006 12-13 ATCAACG 217630 0.0 43.493725 10-11 ACGCAGA 220440 0.0 42.848522 14-15 TCAACGC 212650 0.0 42.35923 12-13 AGAGTAC 221120 0.0 42.13349 18-19 AACGCAG 216850 0.0 41.41277 14-15 GAGTACT 213495 0.0 40.45356 20-21 CGCAGAG 224025 0.0 39.961414 16-17 TATCAAC 226225 0.0 39.709217 10-11 AGTACTT 229520 0.0 39.70397 20-21 GTACTTT 227510 0.0 37.938675 22-23 >>END_MODULE