FastQCFastQC Report
Thu 26 May 2016
SRR1660673_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660673_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56531656
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14852162.6272288927817717No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1338870.23683544667433767No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1003290.17747401562055778No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1995100.080.689598
GTGGTAT2039550.079.083066
TGGTATC2051150.078.4754947
AGTGGTA2060400.078.421875
GTATCAA2049200.078.410339
AAGCAGT2211650.073.6523061
GCAGTGG2258750.071.9512943
CAGTGGT2262250.071.603244
AGCAGTG2436050.067.01972
CAACGCA1925500.043.82710612-13
ATCAACG1962600.043.07263610-11
ACGCAGA2002900.042.18900314-15
TCAACGC1916400.041.77995712-13
AGAGTAC2034000.041.30576718-19
AACGCAG1961650.040.7471114-15
GAGTACT1949950.039.63214520-21
CGCAGAG2035950.039.18960616-17
TATCAAC2060350.038.96335610-11
AGTACTT2105200.038.90110820-21
GTACTTT2083650.037.01550322-23
Produced by FastQC (version 0.11.5)