FastQCFastQC Report
Thu 26 May 2016
SRR1660672_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660672_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30188876
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9148273.030344687228501No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA356620.11812960509029882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1238350.080.845458
GTGGTAT1266500.079.227956
GTATCAA1266050.078.927069
TGGTATC1273550.078.487767
AGTGGTA1283100.078.295895
AAGCAGT1340250.075.7799151
GCAGTGG1382000.073.41273
CAGTGGT1403650.071.844854
AGCAGTG1455800.069.911942
CAACGCA1209550.043.75798812-13
ATCAACG1230100.043.0976510-11
ACGCAGA1248300.042.4456214-15
AGAGTAC1251250.041.81080618-19
TCAACGC1203150.041.55582412-13
AACGCAG1232050.040.51318414-15
GAGTACT1201700.039.96470620-21
CGCAGAG1264750.039.3857816-17
TATCAAC1277850.039.06581510-11
AGTACTT1310050.038.9262420-21
GTACTTT1261300.038.039822-23
Produced by FastQC (version 0.11.5)